##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937870_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1851615 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07595585475382 31.0 31.0 34.0 30.0 34.0 2 32.27621076735714 33.0 31.0 34.0 31.0 34.0 3 32.34191935148505 34.0 31.0 34.0 30.0 34.0 4 36.0091314879173 37.0 35.0 37.0 35.0 37.0 5 35.92410571312071 37.0 35.0 37.0 35.0 37.0 6 35.97129964922514 37.0 35.0 37.0 35.0 37.0 7 35.91491913815777 37.0 35.0 37.0 35.0 37.0 8 35.906450855064364 37.0 35.0 37.0 35.0 37.0 9 37.53271657444987 39.0 37.0 39.0 35.0 39.0 10-11 37.4739111532365 39.0 37.0 39.0 34.5 39.0 12-13 37.36419936109829 39.0 37.0 39.0 34.0 39.0 14-15 38.45586123465191 40.0 38.0 41.0 34.0 41.0 16-17 38.44033586895764 40.0 38.0 41.0 34.0 41.0 18-19 38.534335161467155 40.0 38.0 41.0 34.0 41.0 20-21 38.48450433810484 40.0 38.0 41.0 34.0 41.0 22-23 38.329659783486306 40.0 38.0 41.0 34.0 41.0 24-25 38.38374068043303 40.0 38.0 41.0 34.0 41.0 26-27 38.22084504608139 40.0 38.0 41.0 34.0 41.0 28-29 38.05297429541238 40.0 37.0 41.0 33.0 41.0 30-31 37.9701511923375 40.0 37.0 41.0 33.0 41.0 32-33 37.78067497832973 40.0 36.5 41.0 33.0 41.0 34-35 37.60389173775326 40.0 36.0 41.0 33.0 41.0 36-37 37.384085784571845 40.0 36.0 41.0 33.0 41.0 38-39 37.200187133934435 40.0 35.5 41.0 32.5 41.0 40-41 36.958057695579264 39.5 35.0 41.0 32.0 41.0 42-43 36.65264566338035 39.0 35.0 41.0 31.0 41.0 44-45 36.192194111626875 39.0 35.0 41.0 30.0 41.0 46-47 35.60039425042463 39.0 35.0 41.0 28.0 41.0 48-49 35.0683946176716 39.0 35.0 41.0 24.5 41.0 50-51 34.742940892140105 39.0 35.0 40.0 23.5 41.0 52-53 34.46445022318355 38.5 34.5 40.0 22.5 41.0 54-55 34.24487163908264 38.0 34.0 40.0 21.5 41.0 56-57 33.99824234519595 38.0 34.0 40.0 20.0 41.0 58-59 33.74745883998563 38.0 34.0 40.0 18.5 41.0 60-61 33.41119509185225 38.0 33.0 40.0 17.0 41.0 62-63 33.063138665435304 37.0 33.0 40.0 13.5 41.0 64-65 32.678818490884986 36.5 32.0 39.5 9.5 41.0 66-67 32.26543692938327 36.0 32.0 39.0 7.5 40.5 68-69 31.83627697982572 35.5 31.0 39.0 6.5 40.0 70-71 31.427196798470526 35.0 31.0 38.5 2.0 40.0 72-73 30.928139759075187 35.0 30.5 37.5 2.0 39.5 74-75 30.422033738115104 35.0 30.0 37.0 2.0 39.0 76-77 29.174281640621835 33.5 28.5 35.5 2.0 38.0 78-79 29.749964760492865 34.0 29.5 36.0 2.0 38.5 80-81 29.56192486018962 34.0 29.5 36.0 2.0 37.5 82-83 29.201887541416546 34.0 29.0 35.0 2.0 37.0 84-85 28.797010987705328 34.0 29.0 35.0 2.0 36.5 86-87 28.359681953321832 34.0 29.0 35.0 2.0 36.0 88-89 27.974826840352883 34.0 28.0 35.0 2.0 36.0 90-91 27.42913375620742 33.0 26.5 35.0 2.0 35.0 92-93 26.965411006067676 33.0 26.0 35.0 2.0 35.0 94-95 26.492984772752436 33.0 25.0 35.0 2.0 35.0 96-97 25.940141714125236 32.5 24.5 35.0 2.0 35.0 98-99 25.08228276396551 32.0 19.0 34.0 2.0 35.0 100-101 22.590410533507235 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2501399329113241 1101 2 0.17813946213932752 1101 3 0.19254397253765632 1101 4 0.12821070536790558 1101 5 0.11846903911018813 1101 6 0.13270713605416518 1101 7 0.07020594750648712 1101 8 0.06326731094421234 1101 9 0.12422473140939161 1101 10-11 0.22483622010818038 1101 12-13 0.1987145618489805 1101 14-15 0.2663224736223171 1101 16-17 0.2772467430396972 1101 18-19 0.19722082423466958 1101 20-21 0.2425439044802502 1101 22-23 0.21890148727793957 1101 24-25 0.1331447299084445 1101 26-27 0.06796614304646909 1101 28-29 0.25349624074612365 1101 30-31 0.28312309070965824 1101 32-33 0.3049279944492511 1101 34-35 0.20432469347833404 1101 36-37 0.18878546330677182 1101 38-39 0.13665443708656966 1101 40-41 0.18049573074255676 1101 42-43 0.33718621981775243 1101 44-45 0.5711991108366519 1101 46-47 0.6525189305432448 1101 48-49 0.5669193640228585 1101 50-51 0.3638974732947702 1101 52-53 0.4865633637521327 1101 54-55 0.6735921330564096 1101 56-57 0.5791568557981606 1101 58-59 0.519643664243965 1101 60-61 0.41584755017877484 1101 62-63 0.6195592485744683 1101 64-65 0.6192419916481029 1101 66-67 0.4518792586152216 1101 68-69 0.517188078517318 1101 70-71 0.5476911650365786 1101 72-73 0.4917371282915042 1101 74-75 0.5479891613894345 1101 76-77 0.3574929263534976 1101 78-79 0.48551686250617365 1101 80-81 0.5956967493825687 1101 82-83 0.5513916730649981 1101 84-85 0.5803976948164582 1101 86-87 0.18443576441851306 1101 88-89 0.3464724625746243 1101 90-91 0.3224696635736315 1101 92-93 0.6013190948470211 1101 94-95 0.49025400257935203 1101 96-97 0.7795412597671358 1101 98-99 0.7785212913654007 1101 100-101 1.148889464136893 1102 1 0.24906158147395985 1102 2 0.1941201044159655 1102 3 0.18873818100139772 1102 4 0.16120825935123406 1102 5 0.11236977817544158 1102 6 0.2020267225984398 1102 7 0.1901475587213426 1102 8 0.19625697455116864 1102 9 0.24687011159402772 1102 10-11 0.23744504154896617 1102 12-13 0.2303076288382755 1102 14-15 0.3614896180951064 1102 16-17 0.2957102237477258 1102 18-19 0.3044392035513681 1102 20-21 0.348498508514858 1102 22-23 0.36568352878131805 1102 24-25 0.2977537617592674 1102 26-27 0.46105680347097433 1102 28-29 0.3171097701114718 1102 30-31 0.27415699302679997 1102 32-33 0.32143203169182755 1102 34-35 0.23940662188631734 1102 36-37 0.23513616423520034 1102 38-39 0.195586343138487 1102 40-41 0.20410110671898707 1102 42-43 0.18195358522108762 1102 44-45 0.14894693440783868 1102 46-47 0.24566087841416362 1102 48-49 0.3759222834621099 1102 50-51 0.20329543757766544 1102 52-53 0.2162535349732977 1102 54-55 0.257457628440541 1102 56-57 0.028320475902845033 1102 58-59 0.1538103089929237 1102 60-61 0.11262170130578397 1102 62-63 0.24088562084243392 1102 64-65 0.285651344374223 1102 66-67 0.3591199939982985 1102 68-69 0.3207887202679238 1102 70-71 0.3340776356712283 1102 72-73 0.38396642262884484 1102 74-75 0.3418793799528572 1102 76-77 0.14834008976319168 1102 78-79 0.39638454089443087 1102 80-81 0.3329472162298366 1102 82-83 0.35942013102244275 1102 84-85 0.41670657675012635 1102 86-87 0.37897562396981854 1102 88-89 0.40567395648588445 1102 90-91 0.5507947996826239 1102 92-93 0.5190329897998076 1102 94-95 0.4013399813805414 1102 96-97 0.5930416937378347 1102 98-99 0.5826887251519928 1102 100-101 0.972156868992105 1103 1 0.13873844169434335 1103 2 0.16372267962953657 1103 3 0.18840142230386192 1103 4 0.017486580510080785 1103 5 0.005763364903188517 1103 6 -0.0015959095669657586 1103 7 0.06184249496004668 1103 8 0.08325214602572117 1103 9 0.12950970550265595 1103 10-11 0.11850360289600559 1103 12-13 0.08223408668153809 1103 14-15 0.1662921437853413 1103 16-17 0.26315853376381426 1103 18-19 0.21046990802084764 1103 20-21 0.22727156781905222 1103 22-23 0.17035605570963241 1103 24-25 0.13794442661007622 1103 26-27 0.15999824212394032 1103 28-29 0.01225524491647434 1103 30-31 0.24995741397517435 1103 32-33 0.27714207254857826 1103 34-35 0.15253649683989323 1103 36-37 0.1470452889102063 1103 38-39 0.18850202965575846 1103 40-41 0.2707434244112008 1103 42-43 0.2735643317854013 1103 44-45 0.2875355192784568 1103 46-47 0.4037232778198856 1103 48-49 0.26043509762578765 1103 50-51 0.35162526300789665 1103 52-53 0.3624018573702301 1103 54-55 0.3014677807248063 1103 56-57 0.21942856058775106 1103 58-59 0.25253384495925246 1103 60-61 0.23557205415956872 1103 62-63 0.3398221071616021 1103 64-65 0.2794757841414608 1103 66-67 0.28519279330497227 1103 68-69 0.3490318798568772 1103 70-71 0.44649313647598277 1103 72-73 0.4259201183079355 1103 74-75 0.44988730368691776 1103 76-77 0.3967157242809556 1103 78-79 0.4339207548352526 1103 80-81 0.32625785136664476 1103 82-83 0.3950249347862176 1103 84-85 0.29312232517157 1103 86-87 0.264304335124951 1103 88-89 0.2337314035578153 1103 90-91 0.5304704218195582 1103 92-93 0.5209235432231054 1103 94-95 0.6824579707330223 1103 96-97 0.7684620230680324 1103 98-99 0.8968771614526005 1103 100-101 1.205235982524357 1104 1 0.180157022179813 1104 2 0.22330002437378482 1104 3 0.10902209504960325 1104 4 0.05360352291454262 1104 5 0.05382720686281317 1104 6 0.14052156991323983 1104 7 0.1913681169754824 1104 8 0.16231413157137808 1104 9 0.2841482885567501 1104 10-11 0.2440016238886571 1104 12-13 0.21498905171323912 1104 14-15 0.2682935682036103 1104 16-17 0.26081921865214497 1104 18-19 0.23077172123525003 1104 20-21 0.17702404595657129 1104 22-23 0.29748620317195673 1104 24-25 0.2452137467422375 1104 26-27 0.25696551553051705 1104 28-29 0.15754322259279974 1104 30-31 0.3322201374161935 1104 32-33 0.278893523236853 1104 34-35 0.34062450720778514 1104 36-37 0.32702660801492556 1104 38-39 0.2540280719913355 1104 40-41 0.3373280897312867 1104 42-43 0.509391603557404 1104 44-45 0.5785644863416977 1104 46-47 0.5250219503087621 1104 48-49 0.35797949394098083 1104 50-51 0.5005672238013688 1104 52-53 0.46519371717011637 1104 54-55 0.5073605523858902 1104 56-57 0.5657296265663376 1104 58-59 0.5552889267465417 1104 60-61 0.5704079541700793 1104 62-63 0.6809350214980299 1104 64-65 0.6908420581201469 1104 66-67 0.5625663761839377 1104 68-69 0.5974517096272187 1104 70-71 0.6992688840036827 1104 72-73 0.6808884191637539 1104 74-75 0.41025858648064784 1104 76-77 0.4601144796663057 1104 78-79 0.7252737186760427 1104 80-81 0.8545163249039902 1104 82-83 0.8931730742858015 1104 84-85 0.8498810730845641 1104 86-87 0.8876396922219385 1104 88-89 0.8471029548894116 1104 90-91 0.7807964034422241 1104 92-93 0.7464306711522148 1104 94-95 0.7974998276140148 1104 96-97 1.0287483077882271 1104 98-99 0.9176771707930484 1104 100-101 1.0467418961952681 1105 1 0.1433845608063038 1105 2 0.12937496756896394 1105 3 0.10507367626343012 1105 4 0.03232574629303997 1105 5 -0.10374050629260978 1105 6 -0.04867489564434635 1105 7 0.04155423625697807 1105 8 -0.03986693172886646 1105 9 0.10288043949049808 1105 10-11 0.024549053813338162 1105 12-13 0.124986341553317 1105 14-15 0.014378438954047112 1105 16-17 0.067171578382343 1105 18-19 0.21182488356218698 1105 20-21 0.051937185442973544 1105 22-23 0.11283054230583645 1105 24-25 0.09198620587779516 1105 26-27 -0.0549625898125683 1105 28-29 0.07354180462639448 1105 30-31 0.02563581395692438 1105 32-33 0.08987665333057038 1105 34-35 0.04116179473382431 1105 36-37 0.1437524349437993 1105 38-39 0.10962701397713204 1105 40-41 0.055204022593621005 1105 42-43 0.033178124417013066 1105 44-45 0.13488087099283774 1105 46-47 0.08479843261070386 1105 48-49 0.048660504985051034 1105 50-51 0.17158592390482852 1105 52-53 0.18448101448719 1105 54-55 0.3316734843246181 1105 56-57 0.11159768452303354 1105 58-59 0.2517490013464325 1105 60-61 0.23401399762528285 1105 62-63 0.09623301264513628 1105 64-65 0.026081902797322698 1105 66-67 0.07404181096765683 1105 68-69 -0.019610750047679204 1105 70-71 0.12535481106748492 1105 72-73 0.19422739661213484 1105 74-75 0.15901557196819738 1105 76-77 0.17766009080737177 1105 78-79 0.5117504906361177 1105 80-81 0.3271849999088161 1105 82-83 0.5241015491147714 1105 84-85 0.5145311211669608 1105 86-87 0.35392964442151964 1105 88-89 0.2954443079238871 1105 90-91 0.34155344443098556 1105 92-93 0.3579850832634719 1105 94-95 0.6456470606884359 1105 96-97 0.9495217842967811 1105 98-99 0.8200518840834192 1105 100-101 0.8346233187539589 1106 1 0.024138404314655304 1106 2 -0.0015763578435965542 1106 3 0.06708191945366337 1106 4 0.09194177161371186 1106 5 0.039440591330624386 1106 6 -0.02514230709990528 1106 7 -0.019317209740449925 1106 8 -0.00816033878878386 1106 9 0.0757166020543778 1106 10-11 -0.1002521208681344 1106 12-13 -0.2255950881619313 1106 14-15 0.0425763695586312 1106 16-17 0.10285669281437038 1106 18-19 0.03548462627147586 1106 20-21 0.03676600759104076 1106 22-23 0.016643084959241605 1106 24-25 0.06135602365708337 1106 26-27 0.15900300803366463 1106 28-29 0.12171160413523552 1106 30-31 0.16448339285565083 1106 32-33 0.12373841868446078 1106 34-35 0.09896790511948694 1106 36-37 -0.05962067688738415 1106 38-39 -0.07479018602501952 1106 40-41 -0.04137436831845065 1106 42-43 -0.12152678119469584 1106 44-45 0.0018119887672867208 1106 46-47 0.1510146524577678 1106 48-49 0.15820586671278392 1106 50-51 0.13164185024273678 1106 52-53 0.19023149945976314 1106 54-55 0.18778004126120607 1106 56-57 -0.03291608010003699 1106 58-59 0.032188082880935553 1106 60-61 0.031351887819973 1106 62-63 0.13937541993611546 1106 64-65 0.07941410766263601 1106 66-67 0.15609084021647845 1106 68-69 0.03201968473493011 1106 70-71 0.06879572501910758 1106 72-73 0.14523677095721865 1106 74-75 -0.05512063949596424 1106 76-77 0.020748291330765056 1106 78-79 -0.01277488108662439 1106 80-81 -0.062438064902863744 1106 82-83 -0.1429577303852625 1106 84-85 -0.06601630673198855 1106 86-87 0.019929977588272862 1106 88-89 -0.47698231478626596 1106 90-91 -0.30986412635351 1106 92-93 -0.1488290499924787 1106 94-95 -0.013905219623019605 1106 96-97 0.1542077477666588 1106 98-99 0.45670801838186037 1106 100-101 0.6991194980836504 1107 1 0.1306011043371953 1107 2 0.21396905572412805 1107 3 0.16185316128373017 1107 4 0.09715362647060033 1107 5 0.08598104659319006 1107 6 0.052274866666152775 1107 7 0.15963253107541675 1107 8 0.10388045454004668 1107 9 0.22125412740935246 1107 10-11 0.1761452196772808 1107 12-13 0.22794897314074802 1107 14-15 0.2108347648707749 1107 16-17 0.2650721537113725 1107 18-19 0.32212255730596695 1107 20-21 0.15041261565955466 1107 22-23 0.19867768040661815 1107 24-25 0.18070749987975177 1107 26-27 0.1639894853832331 1107 28-29 0.3101694585413668 1107 30-31 0.21360160335823508 1107 32-33 0.11470923229725827 1107 34-35 0.03269283187180605 1107 36-37 -0.07022750582585502 1107 38-39 0.006571094119230736 1107 40-41 0.1294195239510998 1107 42-43 0.20191811400476212 1107 44-45 0.31648124727528426 1107 46-47 0.2765499240376599 1107 48-49 0.28551598616287066 1107 50-51 0.36796425276696 1107 52-53 0.1271257974088229 1107 54-55 0.32721828782420204 1107 56-57 0.22812741775888412 1107 58-59 0.1701754617405271 1107 60-61 0.3791093816220865 1107 62-63 0.40616604423181 1107 64-65 0.5194918629026617 1107 66-67 0.30807979671861574 1107 68-69 0.4132316522805688 1107 70-71 0.36784350505066143 1107 72-73 0.4947550265353158 1107 74-75 0.43800534653063394 1107 76-77 0.42441349304363385 1107 78-79 0.4652226397607926 1107 80-81 0.32394349921703736 1107 82-83 0.1978834911608267 1107 84-85 0.5037521178792304 1107 86-87 0.5372277012754445 1107 88-89 0.6522575995702837 1107 90-91 0.7191813999029648 1107 92-93 0.8304221332394341 1107 94-95 0.8459240569151838 1107 96-97 0.702804772783562 1107 98-99 0.7161457015010839 1107 100-101 0.9865180782053535 1108 1 0.023848051944078463 1108 2 0.0029965818441795022 1108 3 -0.053101932196106816 1108 4 -0.05139757144091561 1108 5 -0.16956905594631877 1108 6 -0.08991776205871815 1108 7 0.0029742740791220967 1108 8 -0.09260353194228088 1108 9 0.0010092455210539697 1108 10-11 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70-71 -0.40120013290778545 2316 72-73 -0.2489267891546163 2316 74-75 -0.15098374397975434 2316 76-77 -0.31569806882249196 2316 78-79 -0.45478585758035805 2316 80-81 -0.4044568077836743 2316 82-83 -0.2719509477405495 2316 84-85 -0.34632463229893773 2316 86-87 -0.3023233101822598 2316 88-89 -0.2303283332184911 2316 90-91 0.07951663371690643 2316 92-93 -0.11949152281012232 2316 94-95 -0.24691624789266697 2316 96-97 0.05732206898442982 2316 98-99 0.2903261192413389 2316 100-101 0.6166987152318306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 24.0 5 42.0 6 121.0 7 285.0 8 623.0 9 1273.0 10 2229.0 11 3857.0 12 6206.0 13 9100.0 14 11651.0 15 16880.0 16 23983.0 17 34307.0 18 30855.0 19 16378.0 20 11997.0 21 11466.0 22 11671.0 23 12516.0 24 13634.0 25 16398.0 26 19689.0 27 24109.0 28 29289.0 29 36539.0 30 45000.0 31 56105.0 32 70196.0 33 90716.0 34 122161.0 35 172242.0 36 248506.0 37 368455.0 38 303115.0 39 29988.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47797765057126 20.055390110267048 15.626936209542505 19.839696029619176 2 15.282604645134112 21.81808853352344 40.87761224660634 22.02169457473611 3 19.57302138943571 27.432484614782233 30.700550600421796 22.293943395360266 4 12.056156534043346 17.827512273102865 38.06103877166358 32.05529242119021 5 12.083818393480792 39.75118102579755 33.41650708075272 14.748493499968937 6 28.87365894097855 37.95027584027997 17.72263672523716 15.45342849350432 7 26.30595453158459 36.09513856822287 20.200257612948697 17.39864928724384 8 26.557734734272515 34.53363685215339 19.545585880434107 19.36304253313999 9 27.643651623042587 16.544206003947906 20.87977252290568 34.932369850103825 10-11 24.3665124769458 28.51383791986995 26.17415067387119 20.945498929313057 12-13 26.358924310492142 26.231840772374106 27.672669964105516 19.73656495302824 14-15 23.259532894257177 26.33730554137874 28.424861539790943 21.978300024573144 16-17 19.817051600899756 29.967541848602437 28.91894373290344 21.29646281759437 18-19 19.539321079166026 27.33375998790245 33.77913335115561 19.34778558177591 20-21 21.38573623566454 25.800665905169275 33.95452078320817 18.859077075958012 22-23 20.53479736562759 26.214457399916885 33.66661292931486 19.584132305140667 24-25 19.90668143936008 26.357376224910745 34.36735886438565 19.368583471343523 26-27 20.072266955136808 26.373361085802767 34.24509088591694 19.30928107314349 28-29 19.687263463492602 26.57388337216031 34.44957186807775 19.28928129626934 30-31 19.836710115223738 26.655649257540038 34.311398427858926 19.196242199377302 32-33 19.62894554213484 26.65049159787537 34.03296581632791 19.687597043661885 34-35 19.987308376741385 26.50980360388094 34.095829856638666 19.407058162739013 36-37 20.22107727578357 26.350618244073416 33.963890981656554 19.464413498486458 38-39 20.202796211410327 26.554800207926878 33.91152306435607 19.330880516306735 40-41 20.209337078548526 26.660571060705646 33.827588683775765 19.30250317697006 42-43 19.88752298033043 26.84248436851389 33.47802355249238 19.791969098663298 44-45 20.11317690588403 27.270336238995753 32.83766680375424 19.778820051365983 46-47 20.332979588089316 27.502801608325704 31.828295839037814 20.335922964547166 48-49 20.5922397474637 27.66349916154276 31.49712548234919 20.247135608644346 50-51 21.0124944980463 28.13843590595237 30.866945882378356 19.982123713622972 52-53 20.77027351798295 28.397101989344435 30.568287683994782 20.26433680867783 54-55 20.546792934816363 28.39067516735391 30.59561517918142 20.466916718648314 56-57 20.912365691571953 28.839013509827904 29.951447790172363 20.297173008427777 58-59 20.8308692681794 28.368910383638067 30.333897705516538 20.466322642665997 60-61 20.88757652103704 28.159714627500858 30.648352924339022 20.30435592712308 62-63 21.083567588294542 28.036200830086166 30.68278232785973 20.197449253759554 64-65 20.95597626936485 28.069901140355853 30.702764883628614 20.27135770665068 66-67 20.777327030005434 28.322472091247935 30.426338743836794 20.473862134909833 68-69 20.8145220371314 28.633432409085373 30.2340634263036 20.317982127479628 70-71 20.84331786035434 28.511407609033196 29.88582939757995 20.759445133032514 72-73 20.867053896193326 28.383870297010986 29.86970833569614 20.879367471099552 74-75 20.81112974349419 28.665759350620945 29.648982104811207 20.874128801073656 76-77 20.811453784939093 28.389027956675662 30.098967657963456 20.700550600421792 78-79 20.703142931981 28.50265849002087 29.905622929172644 20.888575648825487 80-81 20.494390299490068 29.026568172416066 29.695606103647954 20.783435424445916 82-83 20.63496049825985 28.914933537269032 29.554446943232854 20.89565902123826 84-85 20.697227352154655 28.629154536427983 29.379692380477373 21.293925730939986 86-87 20.582950559376545 28.675669618144163 29.497708756950015 21.24367106552928 88-89 20.85687359413269 28.246962786540397 29.688434150727876 21.207729468599034 90-91 20.562656047661292 28.497121768825984 29.734862448501744 21.205359735010983 92-93 20.345935506240956 28.544783071995006 29.73675156989326 21.372529851870777 94-95 20.268009278386707 28.64188829751325 29.539159058443577 21.550943365656465 96-97 20.208655687062375 28.595307339808762 29.330449364473715 21.865587608655147 98-99 20.341485254819105 28.464971689166273 29.255617340358654 21.937925715655968 100-101 20.54120189061445 28.47654287643484 29.553004726536123 21.429250506414586 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2745.0 1 1842.0 2 1243.5 3 1541.5 4 1222.5 5 1116.0 6 2149.5 7 4581.0 8 6964.5 9 7684.5 10 6605.0 11 5534.5 12 5494.5 13 5656.0 14 5914.5 15 6137.5 16 6333.0 17 6561.0 18 6955.5 19 7397.0 20 7837.5 21 8462.0 22 9410.0 23 10777.5 24 12315.5 25 14467.0 26 17616.0 27 20728.5 28 23106.0 29 25644.0 30 28871.0 31 32062.5 32 35515.5 33 39159.0 34 43502.0 35 47144.0 36 51412.5 37 55562.0 38 56429.5 39 57175.5 40 58718.5 41 61375.5 42 62620.5 43 62427.0 44 63917.0 45 64989.5 46 66035.5 47 68718.5 48 78589.5 49 76546.5 50 64201.0 51 57987.5 52 51435.0 53 47234.0 54 44008.0 55 39499.5 56 33897.5 57 29164.0 58 25905.5 59 23255.5 60 19751.0 61 16243.0 62 12785.0 63 8936.0 64 5958.0 65 4387.0 66 3276.0 67 2410.5 68 1866.0 69 1419.0 70 1059.0 71 787.5 72 605.0 73 557.5 74 484.0 75 357.0 76 249.5 77 153.0 78 82.5 79 66.5 80 55.5 81 34.0 82 19.5 83 16.5 84 12.0 85 4.5 86 2.5 87 3.5 88 2.0 89 0.5 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020630638658684444 2 0.0 3 0.0 4 2.700345374173357E-4 5 0.025383246517229556 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0041585318762269695 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.911139734772078E-4 24-25 2.7003453741733567E-5 26-27 4.3205525986773707E-4 28-29 0.0013231692333449448 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.3501726870866784E-4 40-41 4.3205525986773707E-4 42-43 0.016391096421232276 44-45 2.7003453741733567E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.7003453741733567E-5 68-69 8.101036122520071E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.400690748346713E-5 82-83 0.002754352281656824 84-85 5.130656210929378E-4 86-87 0.0 88-89 0.0 90-91 0.011746502377654103 92-93 0.0030243868190741596 94-95 0.0 96-97 0.0 98-99 0.0014581865020536126 100-101 0.019712521231465503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1851615.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.30237068645211 #Duplication Level Percentage of deduplicated Percentage of total 1 69.07136351251452 33.36310604200159 2 15.831674001289564 15.294147723947098 3 6.301148530909077 9.130812362710902 4 3.0907445158617666 5.97161149209096 5 1.7053307318123951 4.118575857550048 6 1.092259286169926 3.16552277557796 7 0.6720068763664612 2.2721667672268318 8 0.4518263559254619 1.7459427303856405 9 0.3138745881081792 1.364479803347287 >10 1.3435150546328718 11.079947335660698 >50 0.07960332187565582 2.700296090217645 >100 0.04083627477932976 3.5486159224558804 >500 0.003244068132497246 1.0655880671483446 >1k 0.00223728836723948 2.2390803668455073 >5k 0.0 0.0 >10k+ 3.3559325508592203E-4 2.9401066628336734 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22476 1.2138592525984073 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18475 0.9977776157570553 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13462 0.7270409885424346 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4363 0.23563213735036714 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4342 0.2344979922932143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3748 0.2024178892480348 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3671 0.19825935737180786 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2963 0.16002246687351313 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2152 0.11622286490442127 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2090 0.1128744366404463 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1901 0.10266713112607102 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.400690748346713E-5 2 0.0 0.0 0.0 0.0 5.400690748346713E-5 3 0.0 0.0 0.0 0.0 5.400690748346713E-5 4 0.0 0.0 0.0 5.400690748346713E-5 5.400690748346713E-5 5 0.0 0.0 0.0 1.0801381496693427E-4 5.400690748346713E-5 6 0.0 0.0 0.0 1.0801381496693427E-4 5.400690748346713E-5 7 0.0 0.0 0.0 1.0801381496693427E-4 5.400690748346713E-5 8 0.0 0.0 0.0 1.0801381496693427E-4 5.400690748346713E-5 9 0.0 0.0 0.0 1.620207224504014E-4 5.400690748346713E-5 10-11 0.0 0.0 0.0 1.8902417619213498E-4 5.400690748346713E-5 12-13 0.0 0.0 0.0 2.1602762993386853E-4 1.0801381496693427E-4 14-15 0.0 0.0 0.0 2.970379911590692E-4 1.0801381496693427E-4 16-17 0.0 0.0 0.0 3.7804835238426995E-4 1.0801381496693427E-4 18-19 0.0 0.0 0.0 4.5905871360947065E-4 1.3501726870866784E-4 20-21 0.0 0.0 0.0 5.130656210929378E-4 2.1602762993386853E-4 22-23 0.0 0.0 0.0 7.290932510268063E-4 2.1602762993386853E-4 24-25 0.0 0.0 0.0 9.99127788444142E-4 2.1602762993386853E-4 26-27 0.0 0.0 0.0 0.0015121934095370798 2.1602762993386853E-4 28-29 0.0 0.0 0.0 0.0028893695503654916 2.1602762993386853E-4 30-31 0.0 0.0 0.0 0.005508704563313647 2.1602762993386853E-4 32-33 0.0 0.0 0.0 0.00810103612252007 2.700345374173357E-4 34-35 0.0 0.0 0.0 0.010666364227984759 2.700345374173357E-4 36-37 0.0 0.0 0.0 0.014905906465436929 2.700345374173357E-4 38-39 0.0 0.0 0.0 0.022385863151897126 2.700345374173357E-4 40-41 0.0 0.0 0.0 0.030243868190741596 2.700345374173357E-4 42-43 0.0 0.0 0.0 0.03723776270985059 2.700345374173357E-4 44-45 0.0 0.0 0.0 0.04064019788130902 2.700345374173357E-4 46-47 0.0 0.0 0.0 0.04585186445346359 2.700345374173357E-4 48-49 0.0 0.0 0.0 0.054222935113401004 2.700345374173357E-4 50-51 0.0 0.0 0.0 0.06583442022234644 2.700345374173357E-4 52-53 0.0 0.0 0.0 0.08919240770894597 2.700345374173357E-4 54-55 0.0 0.0 0.0 0.10531346959276092 2.700345374173357E-4 56-57 0.0 0.0 0.0 0.1412550665230083 2.700345374173357E-4 58-59 0.0 0.0 0.0 0.20360604121267112 2.700345374173357E-4 60-61 0.0 0.0 0.0 0.26682112642206934 2.700345374173357E-4 62-63 0.0 0.0 0.0 0.30049443323801117 2.700345374173357E-4 64-65 0.0 0.0 0.0 0.3569046481044926 2.700345374173357E-4 66-67 0.0 0.0 0.0 0.44874339428012844 2.700345374173357E-4 68-69 0.0 0.0 0.0 0.5783059653329661 2.700345374173357E-4 70-71 0.0 0.0 0.0 0.7050871806504051 2.700345374173357E-4 72-73 0.0 0.0 0.0 0.8443709950502669 2.700345374173357E-4 74-75 0.0 0.0 0.0 1.0034753444965612 2.700345374173357E-4 76-77 0.0 0.0 0.0 1.127421197171118 2.700345374173357E-4 78-79 0.0 0.0 0.0 1.286255512079995 2.700345374173357E-4 80-81 0.0 0.0 0.0 1.4907526672661433 2.700345374173357E-4 82-83 0.0 0.0 0.0 1.6798848572732452 3.240414449008028E-4 84-85 0.0 0.0 0.0 1.9151929531787115 3.240414449008028E-4 86-87 0.0 0.0 0.0 2.2044269462064197 3.240414449008028E-4 88-89 0.0 0.0 0.0 2.548910005589715 3.240414449008028E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16495 0.0 29.899155 1 GTATCAA 23615 0.0 28.590384 1 TATCAAC 28420 0.0 23.687145 2 ATCAACG 28610 0.0 23.463417 3 GAGTACT 16470 0.0 23.417866 12-13 TCAACGC 28960 0.0 23.163443 4 CAACGCA 29280 0.0 22.910288 5 AACGCAG 29740 0.0 22.551054 6 TACATGG 16375 0.0 22.368664 2 AGTACTT 16740 0.0 22.330791 12-13 GTACATG 16805 0.0 22.166208 1 GTACTTT 17425 0.0 22.10656 14-15 ACATGGG 16505 0.0 21.185038 3 ACGCAGA 32805 0.0 20.401203 7 CGCAGAG 32825 0.0 20.359833 8 TACTTTT 18770 0.0 19.864542 14-15 ACTTTTT 19755 0.0 19.739641 16-17 GCAGAGT 33610 0.0 19.545128 9 CATGGGA 12695 0.0 18.97327 4 CATGGGG 3585 0.0 18.950201 4 >>END_MODULE