FastQCFastQC Report
Thu 26 May 2016
SRR937869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768568
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179211.013305680075632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138260.781762420217939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96220.5440559820148279No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA39850.22532353859167417No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33130.18732669594836046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.18314251982394794No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26070.14740739400464106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25880.14633307851323782No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25120.14203581654762498No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23770.13440252226660213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA202550.029.7411041
GGTATCA139900.028.2760221
GGACCGA2155.820766E-1126.512416
TATCAAC240900.024.9796562
ATCAACG243700.024.7100523
TCAACGC246300.024.4464364
TACATGG157550.024.2564492
ATGGGCG2751.8189894E-1224.1928125
GTACATG160050.024.1516911
CAACGCA249300.024.1515715
TCGACCG601.7127677E-423.74800918-19
AACGCAG253950.023.6618546
GAGTACT130250.022.88260812-13
ACATGGG158500.022.81963
AGTACTT133350.022.04789512-13
GTACTTT138350.021.45706714-15
ACGCAGA281000.021.3164657
CGCAGAG281400.021.2186438
CATGGGA122350.020.5084724
GCAGAGT286250.020.4105059
Produced by FastQC (version 0.11.5)