FastQCFastQC Report
Thu 26 May 2016
SRR937869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768568
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217351.228960379244677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176310.9969082331015828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124950.7065037928991139No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43590.24647059089613743No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40820.23080820188989057No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36070.20395031460481022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35120.19857873714779414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28860.16318286885208824No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20060.11342509872393935No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.10409551682491146No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18310.10353008761890976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156250.028.8311521
GTATCAA223600.028.1802881
GATCGTC1151.2819719E-424.7830737
TACACCG1801.9513755E-723.7511165
TATCAAC270050.023.2717862
ATCAACG272750.022.9717483
GAGTACT157300.022.82941612-13
TCAACGC276050.022.7315524
AGTACTT159600.022.61947312-13
CAACGCA279950.022.4318475
GTACATG167300.022.0414141
AACGCAG285600.022.0354846
TACATGG164800.021.964022
GTACTTT168000.021.20395714-15
ACATGGG165550.021.0037043
TACTTTT180650.019.9294914-15
CATGGGA128250.019.7416694
ACGCAGA318150.019.7080547
CGCAGAG318300.019.6539978
ATAACGC1707.95407E-519.5597443
Produced by FastQC (version 0.11.5)