##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937869_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1768568 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1393392846642 33.0 31.0 34.0 31.0 34.0 2 32.33280428007292 34.0 31.0 34.0 31.0 34.0 3 32.377157112420896 34.0 31.0 34.0 30.0 34.0 4 36.014068444074525 37.0 35.0 37.0 35.0 37.0 5 35.94354528635597 37.0 35.0 37.0 35.0 37.0 6 36.00553668278517 37.0 35.0 37.0 35.0 37.0 7 35.95580774954652 37.0 35.0 37.0 35.0 37.0 8 35.93502144107549 37.0 35.0 37.0 35.0 37.0 9 37.578927132007365 39.0 37.0 39.0 35.0 39.0 10-11 37.512903659910165 39.0 37.0 39.0 35.0 39.0 12-13 37.39076982055539 39.0 37.0 39.0 34.0 39.0 14-15 38.506025213619154 40.0 38.0 41.0 34.0 41.0 16-17 38.49665718253412 40.0 38.0 41.0 34.0 41.0 18-19 38.57666230532273 40.0 38.0 41.0 34.0 41.0 20-21 38.52656923567542 40.0 38.0 41.0 34.0 41.0 22-23 38.46703321557328 40.0 38.0 41.0 34.0 41.0 24-25 38.46730603516517 40.0 38.0 41.0 34.0 41.0 26-27 38.25122641594781 40.0 38.0 41.0 34.0 41.0 28-29 38.17618943687775 40.0 37.5 41.0 33.5 41.0 30-31 38.06642549226267 40.0 37.0 41.0 34.0 41.0 32-33 37.82841117785689 40.0 36.5 41.0 33.0 41.0 34-35 37.634224129352106 40.0 36.0 41.0 33.0 41.0 36-37 37.41239918397257 40.0 36.0 41.0 33.0 41.0 38-39 37.22570718230794 40.0 35.5 41.0 33.0 41.0 40-41 36.98055206245957 40.0 35.0 41.0 32.0 41.0 42-43 36.666664216473436 39.0 35.0 41.0 31.0 41.0 44-45 36.20175842828775 39.0 35.0 41.0 30.0 41.0 46-47 35.621180808428065 39.0 35.0 41.0 28.0 41.0 48-49 35.068304696228815 39.0 35.0 41.0 24.5 41.0 50-51 34.744888802692344 39.0 35.0 40.0 23.5 41.0 52-53 34.444551467628045 39.0 34.5 40.0 22.5 41.0 54-55 34.22018689696975 38.0 34.0 40.0 20.5 41.0 56-57 33.964697710237886 38.0 34.0 40.0 19.5 41.0 58-59 33.72626526093427 38.0 34.0 40.0 18.0 41.0 60-61 33.39245932302292 38.0 33.0 40.0 15.5 41.0 62-63 32.990141176364155 37.0 33.0 40.0 11.5 41.0 64-65 32.60204922852839 36.5 32.0 39.5 8.0 41.0 66-67 32.19673656879464 36.0 32.0 39.0 7.0 40.5 68-69 31.747474793165996 35.5 31.0 39.0 2.0 40.0 70-71 31.320879943547546 35.0 31.0 38.0 2.0 40.0 72-73 30.843670698553858 35.0 30.5 37.5 2.0 39.5 74-75 30.369910006287572 35.0 30.0 37.0 2.0 39.0 76-77 29.09781331563163 33.5 28.5 35.5 2.0 38.0 78-79 29.673497711142574 34.0 29.5 36.0 2.0 38.5 80-81 29.502420602430895 34.0 29.5 36.0 2.0 37.5 82-83 29.1107364828494 34.0 29.0 35.0 2.0 37.0 84-85 28.69685050277965 34.0 29.0 35.0 2.0 36.5 86-87 28.34991275427351 34.0 29.0 35.0 2.0 36.0 88-89 27.929733547140962 34.0 28.0 35.0 2.0 36.0 90-91 27.409318160229066 33.5 26.5 35.0 2.0 35.0 92-93 26.96940575652166 33.0 26.0 35.0 2.0 35.0 94-95 26.484679130234177 33.0 25.0 35.0 2.0 35.0 96-97 25.96917279968879 33.0 24.5 35.0 2.0 35.0 98-99 25.185604964016086 32.0 20.0 34.0 2.0 35.0 100-101 22.89075794654206 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2763629144250217 1101 2 0.26353483125548394 1101 3 0.23820366080619237 1101 4 0.12365052589405678 1101 5 0.07445592587847472 1101 6 0.0651114504391046 1101 7 0.1437399312598444 1101 8 0.12954623415379274 1101 9 0.1937734899323047 1101 10-11 0.30132197116107307 1101 12-13 0.29976858488623037 1101 14-15 0.37748899475808173 1101 16-17 0.24860817197271956 1101 18-19 0.3723584772086923 1101 20-21 0.2571257692185682 1101 22-23 0.2845891409233161 1101 24-25 0.28190013513886925 1101 26-27 0.14460386225362498 1101 28-29 0.25923652880191383 1101 30-31 0.34070319708241215 1101 32-33 0.36850609309367854 1101 34-35 0.33585803769302913 1101 36-37 0.22720312364390338 1101 38-39 0.3417997670380686 1101 40-41 0.3769065097549458 1101 42-43 0.4832340012757541 1101 44-45 0.48290459263797203 1101 46-47 0.691672188887658 1101 48-49 0.5765228593507246 1101 50-51 0.6961778916050818 1101 52-53 0.7818476655716324 1101 54-55 0.8369946108068547 1101 56-57 0.6984311044094369 1101 58-59 0.8087322563718544 1101 60-61 0.8293478578387266 1101 62-63 0.7427153031078433 1101 64-65 0.7725628188362492 1101 66-67 0.848600459737014 1101 68-69 0.9597338949881831 1101 70-71 0.8563300105265164 1101 72-73 0.924596504396515 1101 74-75 0.9568278026364148 1101 76-77 0.8135727062466351 1101 78-79 1.00663848541436 1101 80-81 1.1227360536627522 1101 82-83 0.908580579823191 1101 84-85 0.955095121846746 1101 86-87 0.8797836816523947 1101 88-89 1.0774973568862158 1101 90-91 1.0118335954939504 1101 92-93 1.1728845738195837 1101 94-95 1.3222456845018549 1101 96-97 1.3116182031061179 1101 98-99 1.4752506578471447 1101 100-101 2.199697570915969 1102 1 0.13768204667014317 1102 2 0.11418252961310316 1102 3 0.1424129458236436 1102 4 0.10103363343813498 1102 5 -0.04260474052669849 1102 6 -0.007409896451640918 1102 7 0.11455302401950007 1102 8 0.062262292848942025 1102 9 0.15126451367788718 1102 10-11 0.20330064438518747 1102 12-13 0.12596466450082033 1102 14-15 0.18312190869666978 1102 16-17 0.2266779504075842 1102 18-19 0.19888142323047475 1102 20-21 0.08328740214256669 1102 22-23 0.22226087150493612 1102 24-25 0.23996229507208966 1102 26-27 0.15642699735506227 1102 28-29 0.1737515344843601 1102 30-31 0.19708677229688476 1102 32-33 0.23993193464895768 1102 34-35 0.20414998471049728 1102 36-37 0.4242334166167794 1102 38-39 0.31250059375864225 1102 40-41 0.3261425223870802 1102 42-43 0.28719587356652454 1102 44-45 0.49866433074254246 1102 46-47 0.3506671752598365 1102 48-49 0.5902727462046542 1102 50-51 0.5706469851291018 1102 52-53 0.7925177165627844 1102 54-55 0.751325188404941 1102 56-57 0.7277145629577575 1102 58-59 0.6434090106387202 1102 60-61 0.6167451463561591 1102 62-63 0.615673016652174 1102 64-65 0.6994572611015855 1102 66-67 0.7017581149482766 1102 68-69 0.7135098305804135 1102 70-71 0.6549267478969014 1102 72-73 0.6236279740884569 1102 74-75 0.6404328122412579 1102 76-77 0.6965016052599999 1102 78-79 0.6367985745231692 1102 80-81 0.6250835830555879 1102 82-83 0.7914070187896876 1102 84-85 0.9216657400768398 1102 86-87 0.7612609166643161 1102 88-89 0.886383723036932 1102 90-91 0.9528761987585916 1102 92-93 1.1884424090587977 1102 94-95 1.1712771541937919 1102 96-97 1.1991433466759034 1102 98-99 1.126923836698385 1102 100-101 1.4217423214414673 1103 1 0.12350576629205534 1103 2 0.08923420749496813 1103 3 0.11724065434700748 1103 4 -0.019283327424354013 1103 5 0.04997157159431964 1103 6 -0.013524475064173203 1103 7 -0.0012878960666995454 1103 8 0.11575702753727768 1103 9 0.09804896384626005 1103 10-11 0.018333386458536438 1103 12-13 0.0645798502448045 1103 14-15 0.09629709145757914 1103 16-17 0.07951884754708516 1103 18-19 0.08884769158066774 1103 20-21 0.16265836615257712 1103 22-23 0.06140031764807219 1103 24-25 0.038134231830980525 1103 26-27 0.05982007337939166 1103 28-29 0.09783249522446624 1103 30-31 0.0927238635348786 1103 32-33 0.07529987543117045 1103 34-35 0.05207359514435694 1103 36-37 -0.022241253094144042 1103 38-39 0.030665512651623317 1103 40-41 0.12012214476087735 1103 42-43 0.23040972151786576 1103 44-45 0.12616934555273218 1103 46-47 0.05603548414537585 1103 48-49 0.09651938785942349 1103 50-51 0.020129704351361966 1103 52-53 0.03802267305010787 1103 54-55 0.020923821928924724 1103 56-57 0.03260091942327392 1103 58-59 0.027905598273122223 1103 60-61 0.03322226371514603 1103 62-63 -0.019025908294899807 1103 64-65 0.04207241529385186 1103 66-67 0.05988834607892102 1103 68-69 0.20680066949266518 1103 70-71 0.05245228088182685 1103 72-73 0.11882828922314204 1103 74-75 0.012241667181775995 1103 76-77 -0.050236258325689676 1103 78-79 0.0827398529603478 1103 80-81 0.023633471094807845 1103 82-83 -0.07378350575276116 1103 84-85 0.03425324827550469 1103 86-87 0.275499344356394 1103 88-89 0.23789908967740203 1103 90-91 0.31791350626923887 1103 92-93 0.4899524931775794 1103 94-95 0.5758872419458037 1103 96-97 0.5127390516272285 1103 98-99 0.6819214607587654 1103 100-101 1.0356400623387998 1104 1 0.07908535186535204 1104 2 0.0869997896104735 1104 3 0.09553678281213251 1104 4 0.06330697144100839 1104 5 0.05047124957183513 1104 6 0.049275165279816235 1104 7 0.03890335982565318 1104 8 0.09877942287529606 1104 9 0.011776372339667773 1104 10-11 0.08130717824349176 1104 12-13 0.044884034761700775 1104 14-15 0.09827980152498128 1104 16-17 0.08566799359765298 1104 18-19 0.05448239123776233 1104 20-21 0.12077979187237275 1104 22-23 0.1332976479567023 1104 24-25 0.03064992473295547 1104 26-27 -0.0368766090064625 1104 28-29 0.051678625710259496 1104 30-31 0.1252783860177047 1104 32-33 0.08831054153532136 1104 34-35 0.12516253712355052 1104 36-37 0.055036784411832684 1104 38-39 0.11256913390365497 1104 40-41 0.052146433922814595 1104 42-43 0.1253965194136626 1104 44-45 0.10122667770215799 1104 46-47 0.15618552770074956 1104 48-49 0.07141156230427015 1104 50-51 0.1949920009031132 1104 52-53 0.2602679441551956 1104 54-55 0.24968838389775527 1104 56-57 0.11673463689427166 1104 58-59 0.14919162315143097 1104 60-61 0.1607227268341731 1104 62-63 0.08689390915795059 1104 64-65 0.31593530916986623 1104 66-67 0.32171470062321816 1104 68-69 0.29584520063516706 1104 70-71 0.4048420568023019 1104 72-73 0.13036795381984945 1104 74-75 0.2875683208554136 1104 76-77 0.16540959590055948 1104 78-79 0.20404223008663536 1104 80-81 0.2808079614945207 1104 82-83 0.11862907454553451 1104 84-85 0.0695974642634738 1104 86-87 0.24851287911204523 1104 88-89 0.3663099058017458 1104 90-91 0.39623026192717603 1104 92-93 0.23391985689385564 1104 94-95 0.16027208322504194 1104 96-97 0.4157167259030601 1104 98-99 0.5988671084561261 1104 100-101 0.7819665882312989 1105 1 0.11464202211048757 1105 2 0.07653910507647765 1105 3 0.1116145652257714 1105 4 0.03164973536069482 1105 5 0.030524504647708284 1105 6 -0.014059230654794419 1105 7 0.04851154123044665 1105 8 -0.01080555319273202 1105 9 0.07313315790828767 1105 10-11 0.07447039379998444 1105 12-13 0.04498400522764001 1105 14-15 0.042505423029524536 1105 16-17 0.12221979775398495 1105 18-19 0.15762120603104535 1105 20-21 0.05478381413075084 1105 22-23 0.013912987856294023 1105 24-25 -0.016962855547660638 1105 26-27 0.0023943215041057897 1105 28-29 0.0021519911404013214 1105 30-31 -0.09124979088665697 1105 32-33 -0.05857338021346692 1105 34-35 0.058921848557922374 1105 36-37 -0.11056646058495545 1105 38-39 -0.03776371032056147 1105 40-41 0.03759106223895259 1105 42-43 0.0923486039092154 1105 44-45 0.07715160960972867 1105 46-47 0.0698855834873271 1105 48-49 -0.03897962341812189 1105 50-51 -0.051955090929070025 1105 52-53 -0.01827462005795155 1105 54-55 0.10625150944565576 1105 56-57 0.15521178080955167 1105 58-59 0.050929445037589005 1105 60-61 0.039548208637903315 1105 62-63 0.21027996626651912 1105 64-65 0.22244979726951186 1105 66-67 0.17679409100261267 1105 68-69 0.22416977532119375 1105 70-71 0.02216167632854038 1105 72-73 0.1561109933908078 1105 74-75 0.25193699505717504 1105 76-77 0.24190962168426822 1105 78-79 0.3630164838827028 1105 80-81 0.39611422506026983 1105 82-83 0.32768431262291386 1105 84-85 0.29221622167629135 1105 86-87 0.4251016242893222 1105 88-89 0.4505117594159209 1105 90-91 0.4751516122433763 1105 92-93 0.3047832126199488 1105 94-95 0.4717213385446932 1105 96-97 0.4563716555135393 1105 98-99 0.5137829832504401 1105 100-101 0.7131891344427359 1106 1 -0.3789250869837879 1106 2 -0.2619686512020678 1106 3 -0.3548936974262631 1106 4 -0.2323286074346811 1106 5 -0.1830453310730462 1106 6 -0.2026215194629657 1106 7 -0.24467237946367248 1106 8 -0.2684298824913043 1106 9 -0.5941590098571439 1106 10-11 -0.5985255357362007 1106 12-13 -0.6314241025909624 1106 14-15 -0.542024379971707 1106 16-17 -0.40000050392775677 1106 18-19 -0.38896710258411815 1106 20-21 -0.4181680511739003 1106 22-23 -0.5043787806531199 1106 24-25 -0.6313742937457505 1106 26-27 -0.4783074926763007 1106 28-29 -0.6169141879794751 1106 30-31 -0.6396846585965861 1106 32-33 -0.8149322626861348 1106 34-35 -1.1790956018515644 1106 36-37 -0.9504791598477951 1106 38-39 -0.7940505319504965 1106 40-41 -1.1310975278030142 1106 42-43 -1.3335056579053557 1106 44-45 -1.2689651386934386 1106 46-47 -1.1855623136019986 1106 48-49 -1.124346159920833 1106 50-51 -1.1487778407318814 1106 52-53 -1.2440368582287817 1106 54-55 -1.261023616904268 1106 56-57 -1.572663349435551 1106 58-59 -1.6200865154652817 1106 60-61 -1.5202384328399248 1106 62-63 -1.6361617861752755 1106 64-65 -2.1617783726772224 1106 66-67 -2.2716931181978026 1106 68-69 -1.9255989483254723 1106 70-71 -1.9994965020902136 1106 72-73 -1.9239376476780272 1106 74-75 -2.202303152570174 1106 76-77 -2.0321178003877733 1106 78-79 -1.900567228419252 1106 80-81 -1.9723433320771626 1106 82-83 -2.0049534063592738 1106 84-85 -1.9004815317782473 1106 86-87 -1.7773598864565123 1106 88-89 -2.123313600157946 1106 90-91 -2.4229543561472333 1106 92-93 -2.3476500580658985 1106 94-95 -2.3446936832196315 1106 96-97 -2.5475510348877606 1106 98-99 -2.6688927137434533 1106 100-101 -2.096554416598739 1107 1 0.03979471321720496 1107 2 0.04986068747281536 1107 3 0.03123247024218756 1107 4 0.049432977955945034 1107 5 0.032325525273449784 1107 6 0.07273162635809882 1107 7 0.006490332684485622 1107 8 0.09073154517871984 1107 9 0.05413359856842703 1107 10-11 0.10074712362792582 1107 12-13 0.14171755273127218 1107 14-15 0.1403537696063637 1107 16-17 0.06472774622044142 1107 18-19 0.06176980800123033 1107 20-21 0.13888451207954944 1107 22-23 0.0755305422399104 1107 24-25 0.04521085457378149 1107 26-27 0.0908911966077568 1107 28-29 0.07922877972304576 1107 30-31 0.004440860958659698 1107 32-33 0.010388746706119889 1107 34-35 0.055659542057476585 1107 36-37 -0.020505123536807446 1107 38-39 0.1292253963713108 1107 40-41 0.18271347572978414 1107 42-43 0.26831627935592195 1107 44-45 0.2214368872966901 1107 46-47 0.02877910009736695 1107 48-49 0.07882411224265695 1107 50-51 0.1308524116651384 1107 52-53 0.12283731235520179 1107 54-55 0.21139657191989158 1107 56-57 0.1626232815653097 1107 58-59 0.16342287067263328 1107 60-61 0.10277298795246992 1107 62-63 0.055098182114171834 1107 64-65 0.10697468982899494 1107 66-67 0.33314812588584175 1107 68-69 0.36670769341526466 1107 70-71 0.3039913161717962 1107 72-73 0.32659114710064685 1107 74-75 0.3813696569760836 1107 76-77 0.3441212143626764 1107 78-79 0.31798753298550864 1107 80-81 0.3957722396257459 1107 82-83 0.446256005184285 1107 84-85 0.4298655367577666 1107 86-87 0.5877249972820628 1107 88-89 0.5497709291743185 1107 90-91 0.7862526939929424 1107 92-93 0.7590330953474904 1107 94-95 0.8267781505344374 1107 96-97 0.8371409865156387 1107 98-99 0.8948424155096077 1107 100-101 1.2935024058941202 1108 1 0.11754047702341097 1108 2 0.1322670715303289 1108 3 0.09358256794573094 1108 4 0.08522912412576034 1108 5 0.10099501955081536 1108 6 0.07346013015514785 1108 7 0.030497488115713622 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-0.41533228358032304 2316 92-93 -0.32292906970398505 2316 94-95 -0.194178646044616 2316 96-97 -0.10372705854957687 2316 98-99 -0.11951353564478495 2316 100-101 0.09015827880670102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 5.0 4 25.0 5 39.0 6 98.0 7 277.0 8 655.0 9 1291.0 10 2283.0 11 3738.0 12 5928.0 13 8868.0 14 11466.0 15 16338.0 16 23001.0 17 33521.0 18 29878.0 19 16405.0 20 11873.0 21 11171.0 22 11688.0 23 12015.0 24 13429.0 25 15703.0 26 18611.0 27 22413.0 28 27870.0 29 33706.0 30 42075.0 31 51262.0 32 64336.0 33 83263.0 34 112052.0 35 160106.0 36 234472.0 37 359152.0 38 300700.0 39 28847.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56647474046407 19.983064336853396 15.601117052357447 19.849343870325086 2 15.296330138281366 21.956351127013495 40.79532141257786 21.95199732212728 3 19.584771408280595 27.5076219856969 30.6476199953861 22.2599866106364 4 12.068464429979509 17.780317183167398 38.10693170972221 32.04428667713088 5 12.082203772253344 39.79493164953805 33.360646374716836 14.762218203491773 6 28.87924015361581 37.94244835369632 17.70601978549878 15.472291707189093 7 26.396553595903576 36.021572255067376 20.191194231717414 17.390679917311633 8 26.616618642879438 34.5592026995852 19.49294570522592 19.33123295230944 9 27.660400957158558 16.52071054095743 20.817237448602484 35.00165105328152 10-11 24.37853675968354 28.52972008992586 26.16232454731738 20.92941860307322 12-13 26.38709903605233 26.29752942819644 27.651873921729507 19.663497614021715 14-15 23.30540866961293 26.317308692682438 28.444594722962307 21.93268791474232 16-17 19.84749808884928 29.958390064730335 28.879155339234906 21.314956507185475 18-19 19.55542563248911 27.31893825965414 33.78286839974488 19.342767708111875 20-21 21.46488020486712 25.762215722273645 33.95104833816872 18.82185573469052 22-23 20.545528155097163 26.236455658697583 33.68481852010384 19.53319766610142 24-25 19.951226076690293 26.32019238163305 34.362970493642315 19.365611048034342 26-27 20.04064355691059 26.411441941572345 34.2371360428284 19.310778458688663 28-29 19.686537339358658 26.52600795234024 34.464014303355995 19.323440404945107 30-31 19.841306639043566 26.693941086800166 34.27261490652324 19.192137367633023 32-33 19.67184750600486 26.65252339746054 34.00044555821433 19.67518353832027 34-35 20.01347983227108 26.5371475679759 34.07988836165757 19.369484238095453 36-37 20.216362770223757 26.345510394770884 33.98091382383666 19.457213011168704 38-39 20.222094937825403 26.535083751373996 33.937343658824545 19.305477651976062 40-41 20.269370549894195 26.698104539408305 33.78584645482856 19.246678455868945 42-43 19.90832944279696 26.914405466187112 33.44162739133668 19.735637699679245 44-45 20.147769268696482 27.322557006572552 32.78296339185149 19.746710332879484 46-47 20.333597577248938 27.527496822287862 31.80748492565737 20.33142067480583 48-49 20.588860592298403 27.66789289413808 31.449144166353797 20.29410234720972 50-51 21.037161138276844 28.193261440894556 30.81201853703109 19.957558883797514 52-53 20.743222765536864 28.48346798087492 30.538294258405667 20.235014995182542 54-55 20.510435561425965 28.501759615689075 30.541828190943182 20.445976631941775 56-57 20.927524415233115 28.843872556780404 29.913212271170803 20.315390756815685 58-59 20.838073514843646 28.437017971601886 30.268838970285568 20.456069543268903 60-61 20.877568744882865 28.28483270080653 30.56204228505774 20.275556269252863 62-63 21.054039200075994 28.137283949500386 30.646771851577093 20.161904998846524 64-65 20.97270220879265 28.11034124783441 30.632636121427055 20.28432042194589 66-67 20.80929316825816 28.337445888424984 30.387466017704718 20.465794925612133 68-69 20.800642101406336 28.658779306195747 30.23624197655957 20.304336615838352 70-71 20.89258089030221 28.51971199297963 29.857687123141435 20.73001999357672 72-73 20.8231179123449 28.442870163884 29.792295235467336 20.941716688303757 74-75 20.856930578863803 28.695051589760755 29.563861836242655 20.884155995132787 76-77 20.82905491900792 28.36272057393326 30.108228804320785 20.699995702738033 78-79 20.72566618869051 28.521097294534336 29.87289716878288 20.880339347992276 80-81 20.513828136661978 29.006687896648586 29.697076957176655 20.78240700951278 82-83 20.606689089723233 28.943751903024285 29.471580514501433 20.977978492751053 84-85 20.70384161627036 28.6552010654963 29.335639533848195 21.305317784385142 86-87 20.638137747601448 28.64911046677312 29.531151756675456 21.181600028949976 88-89 20.862047713178118 28.279630752111313 29.67202844335078 21.18629309135979 90-91 20.562501466732527 28.522865335151565 29.722207221102398 21.19242597701351 92-93 20.34095108150952 28.60524612763133 29.687193865391105 21.366608925468054 94-95 20.308068448597965 28.689142854558035 29.453801041294426 21.548987655549574 96-97 20.301820455871646 28.56008363828815 29.3123023824925 21.825793523347702 98-99 20.393726320824783 28.447776967013063 29.2220211488024 21.936475563359757 100-101 20.52008068579533 28.583344501993675 29.48967422007407 21.406900592136925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2521.0 1 1716.0 2 1134.0 3 1411.5 4 1179.0 5 1071.5 6 2041.5 7 4242.5 8 6479.5 9 7274.0 10 6224.0 11 5201.5 12 5310.5 13 5562.5 14 5808.0 15 5946.5 16 6087.0 17 6384.5 18 6651.5 19 7016.5 20 7572.0 21 8246.0 22 9119.0 23 10330.5 24 11722.5 25 13688.0 26 16640.0 27 19651.5 28 22069.5 29 24540.5 30 27483.5 31 30535.0 32 33807.5 33 37217.5 34 41520.5 35 45129.5 36 49127.0 37 52917.0 38 53907.0 39 54673.5 40 55902.0 41 58166.5 42 59449.0 43 60109.5 44 61574.0 45 62327.0 46 63442.5 47 65760.0 48 75429.5 49 73246.0 50 61127.5 51 55447.5 52 49259.0 53 45294.5 54 41960.5 55 37546.0 56 32354.0 57 27977.5 58 24689.0 59 21986.5 60 18780.5 61 15430.0 62 12205.5 63 8506.0 64 5597.5 65 4167.0 66 3118.5 67 2326.0 68 1783.5 69 1352.5 70 1013.0 71 744.5 72 586.5 73 536.0 74 457.5 75 346.0 76 243.5 77 143.0 78 89.0 79 62.0 80 46.5 81 34.5 82 18.5 83 13.5 84 7.5 85 1.5 86 2.0 87 2.5 88 2.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03963658734071859 2 0.0 3 0.0 4 0.0 5 0.01029081154923079 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006276264186618779 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.958004442011842E-4 22-23 0.0014135730150042294 24-25 0.0 26-27 0.0012439442532037217 28-29 0.0018093734592054135 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.937006663017763E-4 38-39 0.0 40-41 2.8271460300084585E-5 42-43 0.021966924653165725 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.481438090025377E-5 84-85 1.4135730150042293E-4 86-87 0.0 88-89 0.0 90-91 0.010262540088930705 92-93 0.0025161599667075283 94-95 0.0 96-97 0.0 98-99 2.544431427007613E-4 100-101 0.012835242976238403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1768568.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.19735894340008 #Duplication Level Percentage of deduplicated Percentage of total 1 69.84130828748268 34.360079128959484 2 15.60010829932036 15.349682551151567 3 6.081519525082103 8.975840969902807 4 3.0178954356404413 5.938899400034062 5 1.6951147728095584 4.169758496408596 6 0.9772362059874858 2.884646423907168 7 0.6398927513069265 2.203672335992869 8 0.4254678239956275 1.6745514604784204 9 0.29958390910018384 1.326486339870182 >10 1.299656273736525 10.816123114547148 >50 0.07590461023152491 2.5806049687348565 >100 0.040466330972975265 3.5128114027452444 >500 0.0032094447321542796 1.0572278721092787 >1k 0.0022924605229673427 2.225095870369529 >5k 0.0 0.0 >10k+ 3.438690784451014E-4 2.9245196647887406 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21735 1.228960379244677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17631 0.9969082331015828 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12495 0.7065037928991139 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4359 0.24647059089613743 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4082 0.23080820188989057 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3607 0.20395031460481022 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3512 0.19857873714779414 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2886 0.16318286885208824 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2006 0.11342509872393935 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1841 0.10409551682491146 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1831 0.10353008761890976 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.654292060016918E-5 0.0 5 0.0 0.0 0.0 5.654292060016918E-5 0.0 6 0.0 0.0 0.0 5.654292060016918E-5 0.0 7 0.0 0.0 0.0 5.654292060016918E-5 0.0 8 0.0 0.0 0.0 5.654292060016918E-5 0.0 9 0.0 0.0 0.0 5.654292060016918E-5 0.0 10-11 0.0 0.0 0.0 5.654292060016918E-5 0.0 12-13 0.0 0.0 0.0 5.654292060016918E-5 0.0 14-15 0.0 0.0 0.0 5.654292060016918E-5 2.827146030008459E-5 16-17 0.0 0.0 0.0 8.481438090025377E-5 5.654292060016918E-5 18-19 0.0 0.0 0.0 1.1308584120033836E-4 5.654292060016918E-5 20-21 0.0 0.0 0.0 1.6962876180050753E-4 8.481438090025377E-5 22-23 0.0 0.0 0.0 3.1098606330093044E-4 1.1308584120033836E-4 24-25 0.0 0.0 0.0 7.633294281022839E-4 1.1308584120033836E-4 26-27 0.0 0.0 0.0 0.001243944253203722 1.1308584120033836E-4 28-29 0.0 0.0 0.0 0.00240307412550719 1.1308584120033836E-4 30-31 0.0 0.0 0.0 0.0053715774570160715 1.1308584120033836E-4 32-33 0.0 0.0 0.0 0.008735881232726138 1.6962876180050753E-4 34-35 2.827146030008459E-5 0.0 0.0 0.011252041199433665 1.6962876180050753E-4 36-37 5.654292060016918E-5 0.0 0.0 0.0157754748474472 1.6962876180050753E-4 38-39 5.654292060016918E-5 0.0 0.0 0.02284333992246835 1.6962876180050753E-4 40-41 5.654292060016918E-5 0.0 0.0 0.030589720044691526 1.6962876180050753E-4 42-43 5.654292060016918E-5 0.0 0.0 0.03861881476991555 1.6962876180050753E-4 44-45 5.654292060016918E-5 0.0 0.0 0.042689905053127725 1.6962876180050753E-4 46-47 5.654292060016918E-5 0.0 0.0 0.04890962631914634 1.6962876180050753E-4 48-49 5.654292060016918E-5 0.0 0.0 0.058380565519674674 2.261716824006767E-4 50-51 5.654292060016918E-5 0.0 0.0 0.06903890605280658 2.261716824006767E-4 52-53 5.654292060016918E-5 0.0 0.0 0.09004460105576942 2.261716824006767E-4 54-55 5.654292060016918E-5 0.0 0.0 0.10559390422081594 2.261716824006767E-4 56-57 5.654292060016918E-5 0.0 0.0 0.14299704619782783 2.261716824006767E-4 58-59 5.654292060016918E-5 0.0 0.0 0.20793659050712215 2.261716824006767E-4 60-61 5.654292060016918E-5 0.0 0.0 0.2712363901190116 2.261716824006767E-4 62-63 5.654292060016918E-5 0.0 0.0 0.3066039869544174 2.261716824006767E-4 64-65 5.654292060016918E-5 0.0 0.0 0.36187469184108273 2.261716824006767E-4 66-67 5.654292060016918E-5 0.0 0.0 0.4540396524193585 2.261716824006767E-4 68-69 5.654292060016918E-5 0.0 0.0 0.5857563859574526 2.261716824006767E-4 70-71 5.654292060016918E-5 0.0 0.0 0.7186322493678501 2.261716824006767E-4 72-73 5.654292060016918E-5 0.0 0.0 0.8590565926783703 2.261716824006767E-4 74-75 5.654292060016918E-5 0.0 0.0 1.023087605339461 2.544431427007613E-4 76-77 5.654292060016918E-5 0.0 0.0 1.1509594202767435 2.8271460300084586E-4 78-79 5.654292060016918E-5 0.0 0.0 1.3118805723048252 2.8271460300084586E-4 80-81 5.654292060016918E-5 0.0 0.0 1.5179512464321419 2.8271460300084586E-4 82-83 5.654292060016918E-5 0.0 0.0 1.7109039629802192 3.3925752360101507E-4 84-85 5.654292060016918E-5 0.0 0.0 1.9460942412166227 3.3925752360101507E-4 86-87 5.654292060016918E-5 0.0 0.0 2.243623089414713 3.6752898390109964E-4 88-89 5.654292060016918E-5 0.0 0.0 2.6024161920830866 3.958004442011842E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15625 0.0 28.831152 1 GTATCAA 22360 0.0 28.180288 1 GATCGTC 115 1.2819719E-4 24.783073 7 TACACCG 180 1.9513755E-7 23.751116 5 TATCAAC 27005 0.0 23.271786 2 ATCAACG 27275 0.0 22.971748 3 GAGTACT 15730 0.0 22.829416 12-13 TCAACGC 27605 0.0 22.731552 4 AGTACTT 15960 0.0 22.619473 12-13 CAACGCA 27995 0.0 22.431847 5 GTACATG 16730 0.0 22.041414 1 AACGCAG 28560 0.0 22.035484 6 TACATGG 16480 0.0 21.96402 2 GTACTTT 16800 0.0 21.203957 14-15 ACATGGG 16555 0.0 21.003704 3 TACTTTT 18065 0.0 19.92949 14-15 CATGGGA 12825 0.0 19.741669 4 ACGCAGA 31815 0.0 19.708054 7 CGCAGAG 31830 0.0 19.653997 8 ATAACGC 170 7.95407E-5 19.559744 3 >>END_MODULE