FastQCFastQC Report
Thu 26 May 2016
SRR937868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495011
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155291.0387214542234136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117060.7830042722093684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81480.5450127122810468No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33860.22648662785758766No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29660.1983931890802141No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.18427958055158122No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22030.14735677530131885No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21960.14688855132169595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21190.14173808754584413No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20320.13591873237053106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA171050.030.8583971
GGTATCA116600.028.7925191
TACATGG134750.026.8788192
GTACATG137750.026.6501221
GCGTTAT908.8912505E-426.4180641
GTATAGG3700.025.7040621
TATCAAC203550.025.616482
ATCAACG204650.025.385893
ATGGGAG33600.025.3228325
ACATGGG136550.025.3061853
TCAACGC206750.025.2208464
CAACGCA208950.024.9098015
AACGCAG212800.024.4675066
GAGTACT109450.024.2795412-13
CATGGGA106800.023.8111884
CCGCGTG601.7132939E-423.74675694-95
AGTACTT112150.023.01740612-13
GTACTTT117950.022.38628414-15
CGCAGAG234300.022.2237958
ACGCAGA235250.022.1542437
Produced by FastQC (version 0.11.5)