##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937868_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1495011 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64593638441456 33.0 31.0 34.0 30.0 34.0 2 31.699895184717704 33.0 31.0 34.0 30.0 34.0 3 31.719086347859648 33.0 31.0 34.0 30.0 34.0 4 35.36741401902729 37.0 35.0 37.0 33.0 37.0 5 35.38853092050828 37.0 35.0 37.0 33.0 37.0 6 35.47336976115895 37.0 35.0 37.0 33.0 37.0 7 35.392104138364196 37.0 35.0 37.0 33.0 37.0 8 35.40683981589433 37.0 35.0 37.0 33.0 37.0 9 36.93602655766412 39.0 37.0 39.0 33.0 39.0 10-11 36.85128905406047 39.0 37.0 39.0 33.0 39.0 12-13 36.740427996850855 39.0 37.0 39.0 32.5 39.0 14-15 37.7551556476842 40.0 37.5 41.0 32.5 41.0 16-17 37.68618926549704 40.0 37.0 41.0 32.5 41.0 18-19 37.7217485356295 40.0 37.5 41.0 32.0 41.0 20-21 37.78586010403937 40.0 37.5 41.0 32.5 41.0 22-23 37.77918523676414 40.0 37.5 41.0 33.0 41.0 24-25 37.70621620844261 40.0 37.0 41.0 32.0 41.0 26-27 37.58230608336661 40.0 37.0 41.0 32.0 41.0 28-29 37.41732234746099 40.0 37.0 41.0 32.0 41.0 30-31 37.10798616197473 39.0 36.5 41.0 31.5 41.0 32-33 36.8763343547305 39.0 35.5 41.0 31.0 41.0 34-35 36.787513269133136 39.0 35.0 41.0 31.0 41.0 36-37 36.577855614440296 39.0 35.0 41.0 30.0 41.0 38-39 36.36581035189708 39.0 35.0 41.0 30.0 41.0 40-41 36.071828568485444 39.0 35.0 41.0 30.0 41.0 42-43 35.723050867184256 39.0 35.0 40.0 28.5 41.0 44-45 35.32787216950243 39.0 35.0 40.0 26.5 41.0 46-47 34.76008002616703 38.0 34.5 40.0 23.5 41.0 48-49 34.24495973608221 38.0 34.0 40.0 21.0 41.0 50-51 33.311756234569515 37.5 33.0 39.5 16.5 40.5 52-53 33.39363054853777 38.0 33.0 39.5 17.0 40.5 54-55 33.645446421464456 38.0 33.5 40.0 14.5 41.0 56-57 33.41249696490527 38.0 33.0 40.0 11.0 41.0 58-59 33.150380164426885 38.0 33.0 40.0 8.0 41.0 60-61 32.805836880129974 37.5 32.5 40.0 7.5 41.0 62-63 32.43242524636943 37.0 32.0 40.0 4.0 41.0 64-65 31.996337150696547 36.0 31.0 39.0 2.0 41.0 66-67 31.491500062541345 36.0 31.0 39.0 2.0 40.5 68-69 31.02660783097917 35.0 30.5 39.0 2.0 40.0 70-71 30.552977536620133 35.0 30.0 38.0 2.0 40.0 72-73 30.061246706545973 35.0 29.0 37.5 2.0 39.5 74-75 29.52356504400302 34.5 29.0 37.0 2.0 39.0 76-77 28.97309250567387 34.0 28.0 36.0 2.0 39.0 78-79 28.436487758284052 34.0 27.0 36.0 2.0 38.5 80-81 27.89165966002926 34.0 26.0 35.0 2.0 37.0 82-83 27.34130852548911 33.0 26.0 35.0 2.0 37.0 84-85 26.780959805646916 33.0 25.0 35.0 2.0 36.0 86-87 26.186040102714962 32.5 23.5 35.0 2.0 36.0 88-89 25.61216572988426 32.0 21.0 35.0 2.0 35.5 90-91 25.007768170267646 32.0 19.0 34.0 2.0 35.0 92-93 24.332450731131743 31.0 11.5 34.0 2.0 35.0 94-95 23.553294925589178 31.0 2.0 34.0 2.0 35.0 96-97 22.647394567665387 30.5 2.0 34.0 2.0 35.0 98-99 21.457989272319736 29.5 2.0 34.0 2.0 35.0 100-101 18.3642902961918 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16090644722739 1101 2 0.05581225570853121 1101 3 -0.004519653335911755 1101 4 0.1331570057380489 1101 5 -0.021854190556702235 1101 6 0.04956934783510292 1101 7 0.07152864917818391 1101 8 0.07104614444489243 1101 9 0.28153793833063645 1101 10-11 0.3172625240725182 1101 12-13 0.305957491715418 1101 14-15 0.4358364480797192 1101 16-17 0.4366386627887593 1101 18-19 0.5230332934714568 1101 20-21 0.48063121532895536 1101 22-23 0.4056682975473578 1101 24-25 0.3721936004295472 1101 26-27 0.459578128676128 1101 28-29 0.4547169611936184 1101 30-31 0.4687465877995862 1101 32-33 0.41704472568635964 1101 34-35 0.5308843602495088 1101 36-37 0.6311523694203132 1101 38-39 0.7079825406233553 1101 40-41 0.7352009918463907 1101 42-43 0.5585424659189115 1101 44-45 0.55521133416606 1101 46-47 0.616858993083099 1101 48-49 0.7273345450400939 1101 50-51 0.5859739415262268 1101 52-53 0.6503294753953952 1101 54-55 0.7803726897708643 1101 56-57 0.9817553524997962 1101 58-59 0.8308322377149153 1101 60-61 0.8452045133170074 1101 62-63 0.9020145561092718 1101 64-65 0.8530624366891182 1101 66-67 0.8877432347065444 1101 68-69 1.079704314233208 1101 70-71 0.99817545965184 1101 72-73 1.1257471245161632 1101 74-75 1.1804235845420514 1101 76-77 1.0078844183977367 1101 78-79 1.2727303473800262 1101 80-81 1.5076656501898178 1101 82-83 1.6606498109655554 1101 84-85 1.7344300630972853 1101 86-87 2.137109226718948 1101 88-89 1.9396039094994997 1101 90-91 1.8822390247457292 1101 92-93 2.136873191099408 1101 94-95 2.2110122927307536 1101 96-97 2.2978974315211715 1101 98-99 2.739353200764608 1101 100-101 3.3408912812121336 1102 1 0.17211326381300296 1102 2 0.1369142527136873 1102 3 0.09536108291894152 1102 4 0.16795684141911238 1102 5 0.02964124429216497 1102 6 -0.04433097417700793 1102 7 0.06218499732230498 1102 8 -0.013077200770645447 1102 9 0.24623189430906223 1102 10-11 0.26023921463610833 1102 12-13 0.09261055348078884 1102 14-15 0.2493119261285628 1102 16-17 0.4038066789026189 1102 18-19 0.30535774386437 1102 20-21 0.3130649908215801 1102 22-23 0.34723966364730074 1102 24-25 0.3459900304681085 1102 26-27 0.3664391069038686 1102 28-29 0.2911739003558509 1102 30-31 0.37424994706174175 1102 32-33 0.44908029278546735 1102 34-35 0.4617710199370393 1102 36-37 0.5745762990282017 1102 38-39 0.6256110473087517 1102 40-41 0.6595751884725516 1102 42-43 0.849345243439025 1102 44-45 0.8138155508020333 1102 46-47 0.8710577394886272 1102 48-49 0.960866209564422 1102 50-51 0.7065495878694676 1102 52-53 0.609651924807693 1102 54-55 0.7838843444890742 1102 56-57 0.9576475115711318 1102 58-59 0.9703032367475473 1102 60-61 0.9512041422742428 1102 62-63 1.02030696430932 1102 64-65 1.0334607775978988 1102 66-67 1.2019627860098403 1102 68-69 1.1851506919282393 1102 70-71 1.1554280205094756 1102 72-73 1.2037900064583091 1102 74-75 1.179883584807083 1102 76-77 1.1383960598644158 1102 78-79 1.5266190080481685 1102 80-81 1.6271202173286774 1102 82-83 1.5584884868729496 1102 84-85 1.6107813875874335 1102 86-87 1.703804825886749 1102 88-89 1.8268300998179292 1102 90-91 1.9048196271601547 1102 92-93 1.8546037345445434 1102 94-95 2.087592868642126 1102 96-97 2.054411219735261 1102 98-99 2.1960233570001115 1102 100-101 2.2533238640673083 1103 1 0.02630784430317945 1103 2 -0.07624234771913052 1103 3 0.029574974425202072 1103 4 0.01986852846675191 1103 5 0.140007842964728 1103 6 0.09107833652844022 1103 7 -0.009731970485766794 1103 8 0.06855196559401833 1103 9 0.1276498302565443 1103 10-11 0.27517535367542223 1103 12-13 0.22527348047169227 1103 14-15 0.27212895295689776 1103 16-17 0.16874230022032322 1103 18-19 0.22058264213207224 1103 20-21 0.2898168313219074 1103 22-23 0.0887657257531842 1103 24-25 0.026748518513912245 1103 26-27 0.036541848757522644 1103 28-29 0.1369957575268188 1103 30-31 0.01752021327419584 1103 32-33 0.16296493923338318 1103 34-35 0.07161588465638857 1103 36-37 0.15288544613361665 1103 38-39 0.21536779580994647 1103 40-41 0.148024301615429 1103 42-43 0.15815570038011373 1103 44-45 0.23478172287198618 1103 46-47 0.1795936745456217 1103 48-49 0.17240544768530697 1103 50-51 0.06625466775390976 1103 52-53 0.0904280153882695 1103 54-55 0.22699825877508317 1103 56-57 0.26838454005617507 1103 58-59 0.33323359283647136 1103 60-61 0.3584038378539347 1103 62-63 0.3680780433446955 1103 64-65 0.21610338401558593 1103 66-67 0.473183806542675 1103 68-69 0.48820214310191545 1103 70-71 0.4963884717556333 1103 72-73 0.5601073749829055 1103 74-75 0.6040356862391008 1103 76-77 0.649866441011067 1103 78-79 0.6810341379445184 1103 80-81 1.0073832457930116 1103 82-83 1.1499541049807824 1103 84-85 1.232036836607385 1103 86-87 1.4276949601837359 1103 88-89 1.6869875389597588 1103 90-91 1.579266829910292 1103 92-93 1.76500087879214 1103 94-95 2.0503218310244726 1103 96-97 2.026192673911723 1103 98-99 2.4486416046160038 1103 100-101 3.1732508394341146 1104 1 0.06647086534727009 1104 2 0.12411599559501596 1104 3 -0.06040559603661322 1104 4 -0.01733913882960536 1104 5 0.13346651427306 1104 6 0.0665842568947852 1104 7 0.18909214084925452 1104 8 0.20846192478283854 1104 9 0.21539183527101358 1104 10-11 0.20643751620031026 1104 12-13 0.15608930248044572 1104 14-15 0.21982475816942326 1104 16-17 0.17984552891950756 1104 18-19 0.17909762961622988 1104 20-21 0.16304989732540065 1104 22-23 0.12589116573919767 1104 24-25 0.15298904521605294 1104 26-27 0.18415283402116245 1104 28-29 0.20712624858272477 1104 30-31 0.1716758315727276 1104 32-33 0.1995741391349597 1104 34-35 0.34500567820101935 1104 36-37 0.42019346887563813 1104 38-39 0.3980737021455454 1104 40-41 0.48770214340144236 1104 42-43 0.470570295816664 1104 44-45 0.4916120545060423 1104 46-47 0.545557566716262 1104 48-49 0.8267281894971461 1104 50-51 0.6946981746686305 1104 52-53 0.6301486357100785 1104 54-55 0.8359484282624763 1104 56-57 0.8888878372823612 1104 58-59 1.005339801544899 1104 60-61 0.8522961406659775 1104 62-63 0.9499796217023686 1104 64-65 0.9256772864144303 1104 66-67 1.0977935127713074 1104 68-69 1.0847672528976169 1104 70-71 1.0581584660823289 1104 72-73 0.9869928078112018 1104 74-75 1.0467842541875818 1104 76-77 1.0191082416646147 1104 78-79 1.2847442545217511 1104 80-81 1.6001629762399432 1104 82-83 1.562673945477595 1104 84-85 1.8095837989500758 1104 86-87 1.8302942328215366 1104 88-89 1.716874815103214 1104 90-91 1.9205685022129835 1104 92-93 2.0276206739431935 1104 94-95 2.154409913631021 1104 96-97 2.187501686939985 1104 98-99 2.5874335519823894 1104 100-101 3.2276948506424468 1105 1 0.041130109166974904 1105 2 0.10704681183252163 1105 3 -0.028417356480968436 1105 4 0.002194845108114407 1105 5 -0.1361000333501039 1105 6 -0.09732852157095806 1105 7 0.009050760529447643 1105 8 -0.017089088443171363 1105 9 0.10688620063054799 1105 10-11 0.09200079926446847 1105 12-13 0.07021219267728185 1105 14-15 0.16386396563933658 1105 16-17 0.09586873804231288 1105 18-19 0.166128828136884 1105 20-21 0.11436434733202105 1105 22-23 0.09450809552660644 1105 24-25 0.0568776908864308 1105 26-27 0.12019920893658309 1105 28-29 -0.0464072085234406 1105 30-31 -0.05829208560110999 1105 32-33 -0.010072717273850174 1105 34-35 0.06183398316409949 1105 36-37 0.03371060847093332 1105 38-39 -0.014688646889077006 1105 40-41 0.16985864407742213 1105 42-43 0.31223918403564 1105 44-45 0.2948837999803544 1105 46-47 0.35546398145846325 1105 48-49 0.41712775450442763 1105 50-51 0.34997107420524287 1105 52-53 0.41661487740633873 1105 54-55 0.4781298015173405 1105 56-57 0.5334501245450554 1105 58-59 0.6884059717463558 1105 60-61 0.7486206319128925 1105 62-63 0.7395508405300717 1105 64-65 0.7540790818690404 1105 66-67 0.9458352397325349 1105 68-69 0.8325968469334804 1105 70-71 0.8299390801079376 1105 72-73 0.934308067352358 1105 74-75 1.0453497346730174 1105 76-77 1.0644892050794148 1105 78-79 1.2336726384567136 1105 80-81 1.5601858672558286 1105 82-83 1.4063317945411455 1105 84-85 1.651563396248914 1105 86-87 1.5792595266701568 1105 88-89 1.6606709508439756 1105 90-91 1.3516718021494931 1105 92-93 1.5328177852999012 1105 94-95 1.5709968113778778 1105 96-97 1.7598427398263183 1105 98-99 1.9009436372133806 1105 100-101 2.5696263620122544 1106 1 -0.21414906720636395 1106 2 -0.3543125599496477 1106 3 -0.21275556566548204 1106 4 -0.223554731680089 1106 5 -0.3000365943175609 1106 6 -0.2458894867290553 1106 7 -0.23212202518799074 1106 8 -0.09315735762595523 1106 9 -0.19767169434179976 1106 10-11 -0.45965929239365266 1106 12-13 -0.6759846376656995 1106 14-15 -0.8666189002345064 1106 16-17 -0.605217242647413 1106 18-19 -0.326337542946014 1106 20-21 -0.3725677156297351 1106 22-23 -0.4677474928482255 1106 24-25 -0.6097827134120521 1106 26-27 -0.6104364491599057 1106 28-29 -0.2444267321971978 1106 30-31 -0.22503466555529883 1106 32-33 -0.23818832192892359 1106 34-35 -0.22088899395235018 1106 36-37 -0.46166776381432584 1106 38-39 -0.6208182911043494 1106 40-41 -0.34388405118082943 1106 42-43 -0.20965525022010922 1106 44-45 -0.23866738727861048 1106 46-47 -0.12598769667456367 1106 48-49 -0.11812287694306178 1106 50-51 -0.19537038357081826 1106 52-53 0.02487391801918193 1106 54-55 -0.016117328931706254 1106 56-57 -0.18542982557016074 1106 58-59 -0.43992931296001814 1106 60-61 -0.08447946405909335 1106 62-63 -0.3024373342066369 1106 64-65 -0.10180475244418474 1106 66-67 -0.006983600377445498 1106 68-69 -0.06396365012454552 1106 70-71 -0.0262664295905104 1106 72-73 -0.07269536366053231 1106 74-75 0.018511853904300324 1106 76-77 0.4043637014846233 1106 78-79 0.41912459309001804 1106 80-81 0.28928542858410466 1106 82-83 0.28883164969400354 1106 84-85 0.3861178148898503 1106 86-87 0.5204368155561667 1106 88-89 0.6081510981807696 1106 90-91 0.8775404977852084 1106 92-93 0.9313079044302199 1106 94-95 0.867007004027446 1106 96-97 1.088475233658123 1106 98-99 0.9055831915545802 1106 100-101 0.9710145712828187 1107 1 -0.20465079068395653 1107 2 -0.16619388411838898 1107 3 -0.19266645354149858 1107 4 -0.13723847134872358 1107 5 -0.09839644006704873 1107 6 -0.006572562734568521 1107 7 -0.07919854525430026 1107 8 -0.08530829859501665 1107 9 -0.1299040875344275 1107 10-11 -0.06051255267531985 1107 12-13 0.0526826858423064 1107 14-15 0.0714418877996792 1107 16-17 0.07110781779855557 1107 18-19 0.033084134572185064 1107 20-21 0.03877551515254396 1107 22-23 0.021106767313575858 1107 24-25 -0.02416417645303426 1107 26-27 0.08109322125972085 1107 28-29 0.05530107821149244 1107 30-31 -0.09971109323501537 1107 32-33 0.03965234629506398 1107 34-35 0.10638200572312684 1107 36-37 0.04384736448901094 1107 38-39 0.14964168299233194 1107 40-41 0.09858220286474051 1107 42-43 0.020981866382868475 1107 44-45 0.10149271048744879 1107 46-47 0.109892651003058 1107 48-49 -0.01263406127476685 1107 50-51 -0.25900515250202716 1107 52-53 0.07733429557625726 1107 54-55 0.29037282868097947 1107 56-57 0.2506776878565873 1107 58-59 0.11231690898413405 1107 60-61 0.4385041841000472 1107 62-63 0.47462564947075947 1107 64-65 0.48546738744547824 1107 66-67 0.5917764897871827 1107 68-69 0.631616411914802 1107 70-71 0.654843144992654 1107 72-73 0.7219444602202323 1107 74-75 0.6910106186722658 1107 76-77 0.8373264164807743 1107 78-79 0.8639817885724277 1107 80-81 0.9321763393228757 1107 82-83 1.0710444545916609 1107 84-85 1.1409213325967933 1107 86-87 1.2396755910246036 1107 88-89 1.181549901248239 1107 90-91 1.383430873500398 1107 92-93 1.6362703212165108 1107 94-95 1.6368787445497794 1107 96-97 1.66060006664598 1107 98-99 1.8462945390246155 1107 100-101 2.46931228997736 1108 1 -0.012346403092141855 1108 2 -0.0784381558194589 1108 3 -0.04047924403552372 1108 4 -0.10297211424297359 1108 5 -0.012443290344648972 1108 6 -0.040057832395348214 1108 7 -0.0863699399100355 1108 8 -0.061123627847777584 1108 9 0.07466448065654419 1108 10-11 0.07309276913780138 1108 12-13 -0.03303829319647633 1108 14-15 -0.007957941253238232 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-1.0385652310277003 2316 60-61 -0.9316171039533003 2316 62-63 -1.099405682449916 2316 64-65 -0.8479993880906314 2316 66-67 -0.8315085685428159 2316 68-69 -1.0466700844174675 2316 70-71 -1.33808981765565 2316 72-73 -1.2540861907154657 2316 74-75 -1.2477476746339136 2316 76-77 -1.2265737762445852 2316 78-79 -1.0632873624773111 2316 80-81 -1.1628984202662842 2316 82-83 -1.526565486466776 2316 84-85 -1.500913624411897 2316 86-87 -1.990722785189849 2316 88-89 -2.1105040618529536 2316 90-91 -2.0386409737209483 2316 92-93 -2.4181519489758507 2316 94-95 -2.3552572012094366 2316 96-97 -2.248667610038215 2316 98-99 -2.207955501470849 2316 100-101 -2.2049818492543167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5903.0 3 1118.0 4 1038.0 5 1104.0 6 1303.0 7 1692.0 8 2167.0 9 2819.0 10 3561.0 11 5086.0 12 7324.0 13 10143.0 14 11678.0 15 15704.0 16 20217.0 17 26964.0 18 22800.0 19 13630.0 20 12771.0 21 13173.0 22 13951.0 23 15579.0 24 17239.0 25 19391.0 26 22259.0 27 25686.0 28 30059.0 29 35339.0 30 42718.0 31 51865.0 32 64301.0 33 83090.0 34 108886.0 35 148372.0 36 199685.0 37 253848.0 38 168507.0 39 14041.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.45425865390424 19.237409299297216 14.970834815645158 20.337497231153385 2 14.792072127192673 20.979961612490097 41.80602432997962 22.421941930337606 3 19.35758944415707 27.41529582807507 30.2144700710025 23.012644656765364 4 11.741545256884248 16.99696701960871 38.794844805459235 32.46664291804781 5 12.171191659790287 39.48739106808622 33.56880740099691 14.772609871126585 6 28.93327818376471 37.198540458174925 18.504512633563966 15.363668724496396 7 26.653269538335316 35.5356594644813 20.524820660355985 17.2862503368274 8 26.22757076099365 34.28201967223664 20.061712877585812 19.428696689183887 9 27.77717072516717 16.599708391291962 21.00675392582413 34.616366957716735 10-11 24.20443971908862 28.106589994342084 26.665028732010647 21.023941554558643 12-13 26.04706180473528 25.946471738917666 27.835594067968184 20.17087238837888 14-15 23.250612454486983 26.085315605531378 28.284554281617453 22.37951765836419 16-17 20.049568329892484 29.802229401828566 28.672849022709602 21.475353245569348 18-19 19.72769053719925 27.56523382701767 33.023755696293314 19.683319939489767 20-21 21.65148006528172 26.103941367095736 32.97564670122889 19.268931866393654 22-23 20.68941132388866 26.585422501720142 32.65463778456935 20.070528389821845 24-25 20.01255449441516 26.626621426694623 33.49050470005952 19.870319378830693 26-27 20.251804372322432 26.65262539775961 33.26047916835647 19.835091061561492 28-29 19.808910493498168 26.910543107430197 33.49664165421407 19.783904744857566 30-31 19.992095879197684 26.99894142436413 33.345903500003686 19.6630591964345 32-33 19.731869731970292 27.07454699888648 33.01375712878848 20.17982614035476 34-35 20.073911068727238 26.9109363241897 33.155030561640324 19.860122045442736 36-37 20.234022473235616 26.761737318923153 33.00826860637519 19.995971601466042 38-39 20.217069964314284 26.93747241637113 33.01865516216409 19.826802457150496 40-41 20.279693566190147 27.034419853994248 32.93319027191276 19.75269630790285 42-43 19.84050649710149 27.249655322180764 32.6149333801617 20.294904800556044 44-45 20.153046091788106 27.579464757606644 32.06222788538267 20.205261265222582 46-47 20.265669378641206 27.81467630258906 31.126024699369008 20.79362961940073 48-49 20.499170038803033 27.91331129042206 30.896277644143744 20.691241026631165 50-51 20.956582306314424 28.368447726614537 30.415446578927607 20.25952338814343 52-53 20.555757808587284 28.523979722848537 30.341239974227825 20.579022494336357 54-55 20.358419022726977 28.515723063856548 30.380650950026933 20.74520696338955 56-57 20.697418439336086 28.9524147786767 29.73279699507612 20.61736978691109 58-59 20.67763279116903 28.44007437310508 30.11789142196687 20.764401413759025 60-61 20.67132682492709 28.297427195916967 30.377610868073425 20.65363511108252 62-63 20.864155173881084 28.263373702433736 30.36169355892497 20.510777564760208 64-65 20.794065750402588 28.196631995705722 30.42016620884736 20.589136045044327 66-67 20.66797213219782 28.46102501829528 30.110750119958073 20.760252729548824 68-69 20.55348576479485 28.755452816198 30.017654743711358 20.673406675295787 70-71 20.585934136188776 28.54359842797505 29.823976496692335 21.046490939143844 72-73 20.51988865129942 28.429037655417527 29.75039031358794 21.300683379695116 74-75 20.530427637090927 28.63647697065483 29.723130419862116 21.10996497239213 76-77 20.60604496624817 28.423124847930755 29.874007905254913 21.096822280566172 78-79 20.47506334658297 28.507386608694894 29.790973870141933 21.2265761745802 80-81 20.30353937134663 28.992972998876365 29.550602350287075 21.15288527948993 82-83 20.443669310387577 28.86524394943088 29.398478101345987 21.292608638835556 84-85 20.548626791249287 28.564966784260026 29.301720611181675 21.58468581330901 86-87 20.50757618149462 28.641219614269982 29.471397950488853 21.37980625374654 88-89 20.797732540741315 28.16714268432406 29.49496553940458 21.54015923553005 90-91 20.49906877400529 28.27210832379991 29.72547765962366 21.503345242571132 92-93 20.435749820816635 28.362875552084137 29.667672238021186 21.53370238907804 94-95 20.276434848925533 28.491205472805525 29.465419004971256 21.76694067329769 96-97 20.19471691385789 28.353384423652212 29.32730409932285 22.12459456316705 98-99 20.425674659013566 28.27252641784038 29.180650676611048 22.12114824653501 100-101 21.007852657634444 27.650950406771553 29.813530372342882 21.527666563251117 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3005.0 1 1873.0 2 850.0 3 979.0 4 850.5 5 879.0 6 1548.0 7 3150.0 8 4918.0 9 5483.0 10 4745.5 11 4052.0 12 4107.5 13 4295.0 14 4492.5 15 4574.5 16 4625.5 17 4826.0 18 5121.5 19 5317.0 20 5718.0 21 6489.0 22 7203.0 23 8259.5 24 9634.0 25 11511.5 26 14000.5 27 16548.0 28 18610.0 29 20555.0 30 23102.0 31 26047.0 32 29059.0 33 32096.0 34 35873.0 35 38642.0 36 41886.5 37 45207.0 38 46049.5 39 46334.0 40 47276.5 41 49357.5 42 50633.0 43 51026.0 44 51890.5 45 52631.5 46 53679.0 47 55668.5 48 62725.5 49 61705.0 50 52355.5 51 47117.5 52 42134.5 53 38530.0 54 35650.5 55 31919.0 56 27648.0 57 23881.5 58 21089.0 59 18878.5 60 16240.0 61 13391.0 62 10529.0 63 7426.5 64 5056.0 65 3773.0 66 2814.5 67 2095.0 68 1570.5 69 1193.0 70 934.0 71 705.0 72 536.5 73 484.5 74 399.5 75 296.0 76 242.0 77 160.0 78 98.5 79 71.5 80 52.0 81 36.0 82 23.5 83 17.0 84 12.5 85 10.0 86 7.0 87 6.0 88 4.0 89 3.0 90 2.5 91 2.5 92 2.0 93 1.0 94 0.5 95 1.0 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3492281996587316 2 0.29578377684177576 3 0.3010011297575737 4 0.29417843748306866 5 0.2943791049029071 6 0.22140305322168197 7 0.21063390169035545 8 0.21832615278416012 9 0.2197977138629749 10-11 0.22039971612249007 12-13 0.22160372064152037 14-15 0.21856026477397156 16-17 0.20942989717132515 18-19 0.20280787231665853 20-21 0.20173764607752048 22-23 0.209329563461406 24-25 0.20835967093218713 26-27 0.21524925234663825 28-29 0.2243461753793116 30-31 0.22635284957769541 32-33 0.22394484053963482 34-35 0.2243127308093385 36-37 0.2075570012528336 38-39 0.19782463139067205 40-41 0.19250694476495492 42-43 0.2032092071563353 44-45 0.20785800238259117 46-47 0.21618570030588402 48-49 0.2257508473181803 50-51 0.22173749892141262 52-53 0.23277420701252366 54-55 0.21655359057558776 56-57 0.18685481243950713 58-59 0.1696308589033793 60-61 0.18642003302985732 62-63 0.18046689957465198 64-65 0.18675447872958795 66-67 0.1888280420679179 68-69 0.18578458620036908 70-71 0.18698859071939938 72-73 0.196754405151534 74-75 0.2036774311359582 76-77 0.20682122071342618 78-79 0.2020386472072781 80-81 0.19936308160943297 82-83 0.18702203528937245 84-85 0.18203879436338596 86-87 0.18738992555907616 88-89 0.1969550725713724 90-91 0.1934433927242007 92-93 0.18892837577783708 94-95 0.18949693346737917 96-97 0.19715573999121078 98-99 0.19929619246948685 100-101 0.17725622085723786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1495011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.867472073538366 #Duplication Level Percentage of deduplicated Percentage of total 1 73.08627168551409 39.369726989784695 2 14.485208291916893 15.605631062884392 3 5.491056459108407 8.873679913957337 4 2.563918187841473 5.524471655295507 5 1.3678357317433152 3.684092654043548 6 0.789870601717695 2.552899954784213 7 0.5269277976832918 1.986898789853306 8 0.34102056317042706 1.4695932530468245 9 0.22968855757556425 1.1135467764731717 >10 1.0173490151422249 9.453854961379376 >50 0.06506573383833215 2.4142579013430856 >100 0.03145338296108841 3.034586900131951 >500 0.0023527383988093888 0.9102984985320691 >1k 0.0016097683781327397 1.6463015492247375 >5k 1.2382833677944153E-4 0.5434976306909429 >10k+ 2.4765667355888305E-4 1.8166615085748443 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15529 1.0387214542234136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11706 0.7830042722093684 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8148 0.5450127122810468 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3386 0.22648662785758766 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2966 0.1983931890802141 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2755 0.18427958055158122 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2203 0.14735677530131885 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2196 0.14688855132169595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2119 0.14173808754584413 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2032 0.13591873237053106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 6.688913994612748E-5 6.688913994612748E-5 7 0.0 0.0 0.0 6.688913994612748E-5 6.688913994612748E-5 8 0.0 0.0 0.0 6.688913994612748E-5 6.688913994612748E-5 9 0.0 0.0 0.0 6.688913994612748E-5 6.688913994612748E-5 10-11 0.0 0.0 0.0 1.0033370991919123E-4 6.688913994612748E-5 12-13 0.0 0.0 0.0 1.3377827989225497E-4 6.688913994612748E-5 14-15 0.0 0.0 0.0 2.0066741983838245E-4 6.688913994612748E-5 16-17 0.0 0.0 0.0 3.344456997306374E-4 6.688913994612748E-5 18-19 0.0 0.0 0.0 4.013348396767649E-4 6.688913994612748E-5 20-21 0.0 0.0 0.0 4.3477940964982865E-4 6.688913994612748E-5 22-23 0.0 0.0 0.0 7.692251093804661E-4 6.688913994612748E-5 24-25 0.0 0.0 0.0 0.0010702262391380397 6.688913994612748E-5 26-27 0.0 0.0 0.0 0.0015384502187609321 6.688913994612748E-5 28-29 0.0 0.0 0.0 0.0030434558675488006 6.688913994612748E-5 30-31 0.0 0.0 0.0 0.0055852431855016454 6.688913994612748E-5 32-33 0.0 0.0 0.0 0.008394587063239 6.688913994612748E-5 34-35 0.0 0.0 0.0 0.011270820080922481 6.688913994612748E-5 36-37 0.0 0.0 0.0 0.015284168477690131 6.688913994612748E-5 38-39 0.0 0.0 0.0 0.02080252252324565 6.688913994612748E-5 40-41 0.0 0.0 0.0 0.027457991947885334 6.688913994612748E-5 42-43 0.0 0.0 0.0 0.03324390255322536 6.688913994612748E-5 44-45 0.0 0.0 0.0 0.03635424756072029 6.688913994612748E-5 46-47 0.0 0.0 0.0 0.04127059934676066 6.688913994612748E-5 48-49 0.0 0.0 0.0 0.04969863097997272 6.688913994612748E-5 50-51 0.0 0.0 0.0 0.06006644767162248 1.3377827989225497E-4 52-53 0.0 0.0 0.0 0.07966496567583783 1.3377827989225497E-4 54-55 0.0 0.0 0.0 0.0929759045251172 1.3377827989225497E-4 56-57 0.0 0.0 0.0 0.12665458648799238 1.3377827989225497E-4 58-59 0.0 0.0 0.0 0.19203872078533202 1.3377827989225497E-4 60-61 0.0 0.0 0.0 0.2567874082531834 1.3377827989225497E-4 62-63 0.0 0.0 0.0 0.2895296422568129 1.3377827989225497E-4 64-65 0.0 0.0 0.0 0.34504762841209863 1.3377827989225497E-4 66-67 0.0 0.0 0.0 0.4419699921940373 1.3377827989225497E-4 68-69 0.0 0.0 0.0 0.5854471973784807 1.3377827989225497E-4 70-71 0.0 0.0 0.0 0.724610053036399 1.3377827989225497E-4 72-73 0.0 0.0 0.0 0.8750102842052667 1.3377827989225497E-4 74-75 0.0 0.0 0.0 1.0457782584877302 1.3377827989225497E-4 76-77 0.0 0.0 0.0 1.1798575395097428 1.3377827989225497E-4 78-79 0.0 0.0 0.0 1.3501572898125833 1.3377827989225497E-4 80-81 0.0 0.0 0.0 1.5708580070648308 1.3377827989225497E-4 82-83 0.0 0.0 0.0 1.7678465242061763 2.6755655978450994E-4 84-85 0.0 0.0 0.0 2.026306160958013 2.6755655978450994E-4 86-87 0.0 0.0 0.0 2.3460028053305293 2.6755655978450994E-4 88-89 0.0 0.0 0.0 2.7285083521124593 2.6755655978450994E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17105 0.0 30.858397 1 GGTATCA 11660 0.0 28.792519 1 TACATGG 13475 0.0 26.878819 2 GTACATG 13775 0.0 26.650122 1 GCGTTAT 90 8.8912505E-4 26.418064 1 GTATAGG 370 0.0 25.704062 1 TATCAAC 20355 0.0 25.61648 2 ATCAACG 20465 0.0 25.38589 3 ATGGGAG 3360 0.0 25.322832 5 ACATGGG 13655 0.0 25.306185 3 TCAACGC 20675 0.0 25.220846 4 CAACGCA 20895 0.0 24.909801 5 AACGCAG 21280 0.0 24.467506 6 GAGTACT 10945 0.0 24.27954 12-13 CATGGGA 10680 0.0 23.811188 4 CCGCGTG 60 1.7132939E-4 23.746756 94-95 AGTACTT 11215 0.0 23.017406 12-13 GTACTTT 11795 0.0 22.386284 14-15 CGCAGAG 23430 0.0 22.223795 8 ACGCAGA 23525 0.0 22.154243 7 >>END_MODULE