FastQCFastQC Report
Thu 26 May 2016
SRR937868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495011
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185591.24139554826018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149871.0024675403726127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107770.7208642611994159No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37190.24876071145964815No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33980.2272892975369412No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.20454698995525786No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30410.20340987457617368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25790.17250709192106278No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17880.11959778222367595No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.1121730876896558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138750.030.216971
GTATCAA196400.027.9789661
GAGTACT137350.023.82871812-13
AGTACTT139300.023.06889312-13
TATCAAC238500.023.0078662
ATCAACG239350.022.8864613
TACATGG133900.022.672692
TCAACGC241700.022.663944
GTACATG137850.022.6555121
GTACTTT145450.022.33618214-15
CAACGCA245000.022.2811055
AACGCAG250150.021.9494636
CATGGGG27900.021.4560174
ACATGGG134500.021.3352363
TACTTTT156400.020.4534914-15
ACTTTTT164700.019.97067616-17
CGCAGAG276200.019.7780888
ACGCAGA277050.019.7345547
GCAGAGT280750.019.2375979
GTATAGG4250.019.0140781
Produced by FastQC (version 0.11.5)