##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937868_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1495011 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.143876533349925 33.0 31.0 34.0 31.0 34.0 2 32.366957835092855 34.0 31.0 34.0 31.0 34.0 3 32.444588702022926 34.0 31.0 34.0 31.0 34.0 4 36.086042176278305 37.0 35.0 37.0 35.0 37.0 5 35.9313336156055 37.0 35.0 37.0 35.0 37.0 6 36.04558093552489 37.0 35.0 37.0 35.0 37.0 7 36.00263810767947 37.0 35.0 37.0 35.0 37.0 8 36.0116647971152 37.0 35.0 37.0 35.0 37.0 9 37.65125005769188 39.0 37.0 39.0 35.0 39.0 10-11 37.61005169861626 39.0 37.0 39.0 35.0 39.0 12-13 37.5176232817016 39.0 37.0 39.0 35.0 39.0 14-15 38.64123140231075 40.0 38.0 41.0 34.0 41.0 16-17 38.633473265414096 40.0 38.0 41.0 34.5 41.0 18-19 38.72344049642444 40.0 38.0 41.0 35.0 41.0 20-21 38.6992764601732 40.0 38.0 41.0 34.0 41.0 22-23 38.554818325751455 40.0 38.0 41.0 34.0 41.0 24-25 38.491933504168195 40.0 38.0 41.0 34.0 41.0 26-27 38.36493276638099 40.0 38.0 41.0 34.0 41.0 28-29 38.23084713089068 40.0 38.0 41.0 34.0 41.0 30-31 38.167716491718124 40.0 37.0 41.0 34.0 41.0 32-33 37.97079921151082 40.0 37.0 41.0 33.5 41.0 34-35 37.789305563637996 40.0 36.0 41.0 33.0 41.0 36-37 37.57406166242255 40.0 36.0 41.0 33.0 41.0 38-39 37.396185713683714 40.0 36.0 41.0 33.0 41.0 40-41 37.14728787948717 40.0 36.0 41.0 33.0 41.0 42-43 36.85492247214235 40.0 35.0 41.0 31.5 41.0 44-45 36.39403823784574 39.5 35.0 41.0 30.5 41.0 46-47 35.79606370789245 39.0 35.0 41.0 29.0 41.0 48-49 35.249866388942955 39.0 35.0 41.0 25.5 41.0 50-51 34.91478791794843 39.0 35.0 41.0 24.0 41.0 52-53 34.65828980522551 39.0 35.0 40.0 23.0 41.0 54-55 34.4469853399072 38.5 35.0 40.0 22.5 41.0 56-57 34.189605293874095 38.0 34.0 40.0 20.5 41.0 58-59 33.94936492106078 38.0 34.0 40.0 19.5 41.0 60-61 33.6205599825018 38.0 34.0 40.0 18.0 41.0 62-63 33.26067333283835 37.5 33.0 40.0 14.0 41.0 64-65 32.87475209212508 37.0 33.0 40.0 10.0 41.0 66-67 32.47077379363764 36.0 32.0 39.0 8.0 40.5 68-69 32.02518978121231 36.0 32.0 39.0 6.5 40.0 70-71 31.621866661850646 35.0 31.0 38.5 2.0 40.0 72-73 31.17325524695136 35.0 31.0 38.0 2.0 40.0 74-75 30.690158132615746 35.0 30.5 37.0 2.0 39.0 76-77 29.45034886030939 33.5 29.0 36.0 2.0 38.5 78-79 29.975545664881395 34.0 30.0 36.0 2.0 39.0 80-81 29.775200316251855 34.0 30.0 36.0 2.0 37.5 82-83 29.421970473795845 34.0 30.0 35.0 2.0 37.0 84-85 29.0196205914204 34.0 29.0 35.0 2.0 37.0 86-87 28.591827417992242 34.0 29.0 35.0 2.0 36.0 88-89 28.142077215485372 34.0 28.5 35.0 2.0 36.0 90-91 27.630598704624916 33.5 27.0 35.0 2.0 35.0 92-93 27.212916159145315 33.0 26.5 35.0 2.0 35.0 94-95 26.771233121361647 33.0 25.5 35.0 2.0 35.0 96-97 26.257304795750667 33.0 25.0 35.0 2.0 35.0 98-99 25.50966046403672 32.5 22.0 34.5 2.0 35.0 100-101 23.264148892549954 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25854138957512873 1101 2 0.24608011860807721 1101 3 0.22755087616758374 1101 4 0.13143605885341714 1101 5 0.09128955014670481 1101 6 0.1474721040830005 1101 7 0.11971930887661841 1101 8 0.12781299042313066 1101 9 0.23074932740639298 1101 10-11 0.2248481574759893 1101 12-13 0.27144741822466045 1101 14-15 0.3337822227013021 1101 16-17 0.17768604575771008 1101 18-19 0.28362556121490456 1101 20-21 0.3062304280252235 1101 22-23 0.18952451785798985 1101 24-25 0.2368148314980445 1101 26-27 0.26604805595599146 1101 28-29 0.2855020090827409 1101 30-31 0.2248025462286236 1101 32-33 0.28529923682003755 1101 34-35 0.3757781308288912 1101 36-37 0.29712401138264255 1101 38-39 0.48057237218171167 1101 40-41 0.3798818368227117 1101 42-43 0.41086857660831555 1101 44-45 0.45142010067768723 1101 46-47 0.4769799142946667 1101 48-49 0.5453026456701551 1101 50-51 0.6936188185884404 1101 52-53 0.685243486005298 1101 54-55 0.7301078934612875 1101 56-57 0.8092144697322468 1101 58-59 0.7494310740609649 1101 60-61 0.7835662644882575 1101 62-63 0.9252297793287951 1101 64-65 0.6614729330139681 1101 66-67 0.8816361770713712 1101 68-69 0.9492939798754456 1101 70-71 0.9908177447172015 1101 72-73 0.7146686054945519 1101 74-75 0.8582229717881766 1101 76-77 0.7628716697774287 1101 78-79 0.7453898651315072 1101 80-81 0.7887176917478094 1101 82-83 0.7751969167308594 1101 84-85 0.8732921780634086 1101 86-87 1.0064280337988158 1101 88-89 1.022010331969149 1101 90-91 1.1193209144250957 1101 92-93 1.4156905124732724 1101 94-95 1.454748711960665 1101 96-97 1.3645109221301013 1101 98-99 1.6571437639923374 1101 100-101 2.396734632894816 1102 1 0.08169180766081752 1102 2 0.12877759949275003 1102 3 0.08615045476901884 1102 4 0.06834156221268017 1102 5 -0.04240643331539218 1102 6 -0.052953269941355074 1102 7 -0.05735157354257581 1102 8 0.0481819129414518 1102 9 0.16273453870199006 1102 10-11 0.047209447114624936 1102 12-13 0.06607657423489144 1102 14-15 0.09330609532761969 1102 16-17 0.11637520468327267 1102 18-19 0.14866736978334671 1102 20-21 0.07620511760820392 1102 22-23 0.02930825293345407 1102 24-25 -0.06863419650243685 1102 26-27 -0.056078019074149665 1102 28-29 -0.05712815404356064 1102 30-31 0.146139492811713 1102 32-33 0.12088687579542778 1102 34-35 0.09886030354930142 1102 36-37 0.04619375417050264 1102 38-39 0.01917274587478346 1102 40-41 0.008323589734736458 1102 42-43 0.1618173859585923 1102 44-45 0.09986304622715636 1102 46-47 0.308071972654389 1102 48-49 0.35314423691355046 1102 50-51 0.4202073454161521 1102 52-53 0.30171579693913486 1102 54-55 0.38391108177874145 1102 56-57 0.33337730449895275 1102 58-59 0.43182310724279205 1102 60-61 0.3589336630809399 1102 62-63 0.37413719090798025 1102 64-65 0.3702480943513109 1102 66-67 0.5332124462363979 1102 68-69 0.3192958350892532 1102 70-71 0.38936206445005084 1102 72-73 0.45957736872951216 1102 74-75 0.5403307597107343 1102 76-77 0.4523784479336115 1102 78-79 0.5106503238993803 1102 80-81 0.635020515648268 1102 82-83 0.5542038472796058 1102 84-85 0.45520848141736536 1102 86-87 0.585813295450226 1102 88-89 0.5549364016986864 1102 90-91 0.6571460737747081 1102 92-93 0.8470992823339962 1102 94-95 0.8547758775915035 1102 96-97 1.0183266993984326 1102 98-99 1.066818041452816 1102 100-101 1.6612927607190464 1103 1 0.12851946849103513 1103 2 0.15176806306067903 1103 3 0.05637395518836996 1103 4 0.037891765677272815 1103 5 -0.1383112553744681 1103 6 -0.08627797458117925 1103 7 -0.014308716547645872 1103 8 -0.059823098603743574 1103 9 0.1445029721799429 1103 10-11 0.014705460658220204 1103 12-13 0.015728794742877028 1103 14-15 0.14485473009573724 1103 16-17 0.07193333446573291 1103 18-19 0.01609944470801139 1103 20-21 0.05178354207779279 1103 22-23 0.0936895238364599 1103 24-25 0.14404092283239578 1103 26-27 0.07251975782925513 1103 28-29 0.10329086394208531 1103 30-31 0.1524307163949743 1103 32-33 0.12770604599122493 1103 34-35 0.12466031286828638 1103 36-37 0.12517749253471067 1103 38-39 0.12064993506061228 1103 40-41 0.09834877689109334 1103 42-43 0.22593591314416273 1103 44-45 0.19914224831461524 1103 46-47 0.30710357438150737 1103 48-49 0.3048713562069594 1103 50-51 0.24597926833776285 1103 52-53 0.11152597431055966 1103 54-55 0.15221357616146491 1103 56-57 0.3835862051688821 1103 58-59 0.3875690405395318 1103 60-61 0.23565715832307887 1103 62-63 0.3535950328372124 1103 64-65 0.4574678775295027 1103 66-67 0.5106797780027605 1103 68-69 0.3802609199438258 1103 70-71 0.44031350734018204 1103 72-73 0.5796310564605491 1103 74-75 0.7490216229597486 1103 76-77 0.6375142721981888 1103 78-79 0.7130289958399025 1103 80-81 0.572523621977929 1103 82-83 0.5776583188411557 1103 84-85 0.6778515537844925 1103 86-87 0.882290717610779 1103 88-89 1.1008370819848814 1103 90-91 1.2062665941884738 1103 92-93 1.1089615787399723 1103 94-95 1.044362942834784 1103 96-97 1.2686334494527927 1103 98-99 1.443368617676967 1103 100-101 2.001556746968358 1104 1 0.004012069812361574 1104 2 0.140627342774863 1104 3 0.028099198540537884 1104 4 -0.016255235910847432 1104 5 -0.15276699445946207 1104 6 0.03004914116437618 1104 7 0.07966731323821818 1104 8 0.03942717133568152 1104 9 0.12497125353510796 1104 10-11 0.06226042459512371 1104 12-13 0.1103223554421433 1104 14-15 0.02207995108004468 1104 16-17 0.007326942747532428 1104 18-19 0.00746405204019851 1104 20-21 -0.014817656229567433 1104 22-23 0.10770451437079487 1104 24-25 -0.014824020017719874 1104 26-27 -0.00450232230056713 1104 28-29 0.008729329954114462 1104 30-31 0.11185503371766004 1104 32-33 0.17005069610703316 1104 34-35 0.09791101898102283 1104 36-37 0.1718565513357504 1104 38-39 0.05549527654669362 1104 40-41 0.06810526712956033 1104 42-43 0.25357877769454973 1104 44-45 0.4958312201453481 1104 46-47 0.45412473437882994 1104 48-49 0.4175306509686152 1104 50-51 0.4612996777916365 1104 52-53 0.4383103959081538 1104 54-55 0.5522033072794343 1104 56-57 0.6253378019291986 1104 58-59 0.5142761150875543 1104 60-61 0.4721838258980142 1104 62-63 0.5418134733092259 1104 64-65 0.4628186259378211 1104 66-67 0.6826854584229451 1104 68-69 0.6117263504133312 1104 70-71 0.6340101676150631 1104 72-73 0.6348814545068144 1104 74-75 0.6319198929073053 1104 76-77 0.21118168141008553 1104 78-79 0.26025949868976994 1104 80-81 0.4179059039878652 1104 82-83 0.5400079966422737 1104 84-85 0.4478763821111542 1104 86-87 0.5621405275856262 1104 88-89 0.6800635403186384 1104 90-91 0.5652142218733793 1104 92-93 0.6532827344670586 1104 94-95 0.7316495585162812 1104 96-97 0.8148233383658585 1104 98-99 1.0703171634609348 1104 100-101 1.840911017298204 1105 1 0.028293878720468513 1105 2 0.06807835787675032 1105 3 -0.021229831014075273 1105 4 -0.024163257568538654 1105 5 -0.13415192734127857 1105 6 -0.038953638572216676 1105 7 0.0374896124801225 1105 8 -0.09982122445696007 1105 9 -0.08567153644219871 1105 10-11 0.02790395303491522 1105 12-13 -0.14848945710411243 1105 14-15 -0.042034841129293454 1105 16-17 0.07070063039137864 1105 18-19 0.07289341314993436 1105 20-21 0.08545788671209209 1105 22-23 0.030291717014144126 1105 24-25 -0.08531178307351439 1105 26-27 -0.16169654533102573 1105 28-29 -0.19031246543708136 1105 30-31 -0.034349440862982306 1105 32-33 0.050619779866643455 1105 34-35 -0.03299922387670051 1105 36-37 -0.07455424358541762 1105 38-39 -0.1741345859777539 1105 40-41 -0.12123347472588364 1105 42-43 -0.23707551063237275 1105 44-45 -0.12649621279790324 1105 46-47 -0.17032706621463944 1105 48-49 -0.11807618685050159 1105 50-51 0.09000100631074304 1105 52-53 0.03096894274609241 1105 54-55 -0.05893727774666502 1105 56-57 0.03553931103474639 1105 58-59 -0.06475548714352897 1105 60-61 0.01202592438909278 1105 62-63 0.08572834430550103 1105 64-65 0.017822059325993678 1105 66-67 -0.011843393266566693 1105 68-69 -0.0804115473491187 1105 70-71 -0.09953407447803997 1105 72-73 0.047533364429718006 1105 74-75 0.24494362331327935 1105 76-77 0.047898775772910795 1105 78-79 -0.09874357431814218 1105 80-81 0.18021398630780183 1105 82-83 0.19749469837291755 1105 84-85 0.21620128235266733 1105 86-87 0.2531982092995797 1105 88-89 0.44292051559216716 1105 90-91 0.25186173695373526 1105 92-93 0.525733222473189 1105 94-95 0.7248267728667095 1105 96-97 0.7766185393372957 1105 98-99 0.8945665275773607 1105 100-101 1.5989940109696228 1106 1 -0.24559973291824377 1106 2 -0.025450158653320898 1106 3 -0.1840514992286444 1106 4 -0.07164845304334477 1106 5 -0.21217692365556928 1106 6 -0.20337120678087928 1106 7 -0.2155115916155239 1106 8 -0.2472543896911219 1106 9 -0.18672034447929065 1106 10-11 -0.11276943989321353 1106 12-13 -0.2673748282311692 1106 14-15 -0.26704526725524147 1106 16-17 -0.027225215903143862 1106 18-19 0.01580556923835985 1106 20-21 -0.11242496436153004 1106 22-23 -0.1153743130899727 1106 24-25 -0.16960450165071705 1106 26-27 -0.21327317061634687 1106 28-29 -0.24163367214858766 1106 30-31 -0.2413483400247003 1106 32-33 -0.06325230261074921 1106 34-35 -0.32026466472542836 1106 36-37 -0.18275405462443928 1106 38-39 -0.20929716183606217 1106 40-41 -0.1260603264603546 1106 42-43 -0.11654849076049345 1106 44-45 0.16793543283198176 1106 46-47 0.27392958685838664 1106 48-49 0.08888585516722003 1106 50-51 -0.05552261467276054 1106 52-53 0.11204777573131963 1106 54-55 0.012031941805467739 1106 56-57 -0.17084836187114405 1106 58-59 -0.1358543520582245 1106 60-61 0.03900893912564385 1106 62-63 0.06008380320706408 1106 64-65 0.03977342854739163 1106 66-67 -0.1593416680080395 1106 68-69 -0.1920898409866858 1106 70-71 -0.04026836057093419 1106 72-73 0.17854043354388693 1106 74-75 0.16097128940419125 1106 76-77 0.12695821014051845 1106 78-79 0.16266177567557705 1106 80-81 0.23400419142856776 1106 82-83 0.16228479622074587 1106 84-85 0.17067381790045033 1106 86-87 0.2236575669125429 1106 88-89 0.23574937740340474 1106 90-91 0.41910224688451336 1106 92-93 0.6560329786137054 1106 94-95 0.3723147218713372 1106 96-97 0.6374303315582317 1106 98-99 0.8951989789589163 1106 100-101 1.492399421770454 1107 1 0.023863869398439874 1107 2 0.05747352593419919 1107 3 -0.027817305082329824 1107 4 0.025221220475330597 1107 5 -0.257248420932207 1107 6 -0.15732029475571352 1107 7 -0.051191524722959514 1107 8 -0.1517769469284076 1107 9 0.049043789060256415 1107 10-11 0.017361597029257325 1107 12-13 -0.007641053625199845 1107 14-15 0.014768901221628994 1107 16-17 -0.11537400508338891 1107 18-19 -0.0306412017627693 1107 20-21 0.010690831698006775 1107 22-23 0.06109517448608415 1107 24-25 -0.004473420873843281 1107 26-27 -0.2477498853961606 1107 28-29 -0.24817674739394846 1107 30-31 -0.20632651515575873 1107 32-33 -0.11743104141691418 1107 34-35 -0.12809676672489445 1107 36-37 -0.22578296051583635 1107 38-39 -0.3547121851812136 1107 40-41 -0.2647666511407252 1107 42-43 -0.032548473218739105 1107 44-45 -0.054389814726050645 1107 46-47 -0.04700986192114698 1107 48-49 -0.14041253303770418 1107 50-51 -0.07042916096398955 1107 52-53 -0.187640565724827 1107 54-55 -0.21872942890740177 1107 56-57 -0.17894622148447326 1107 58-59 -0.2532491965359327 1107 60-61 -0.2772339966627584 1107 62-63 -0.23878972365082518 1107 64-65 -0.26501722520465165 1107 66-67 -0.1500325500952755 1107 68-69 -0.1174724705544854 1107 70-71 -0.032346210887123306 1107 72-73 -0.016468790496503516 1107 74-75 -0.021269728485474815 1107 76-77 -0.04504412291312221 1107 78-79 0.045004389572739 1107 80-81 0.03807037062930618 1107 82-83 0.0928954540073903 1107 84-85 0.057216805437033 1107 86-87 0.09001233139984066 1107 88-89 0.3195099499663421 1107 90-91 0.3955325578706237 1107 92-93 0.5293898276464226 1107 94-95 0.2931733323887862 1107 96-97 0.4497947849424726 1107 98-99 0.5652540308386627 1107 100-101 1.234992561189415 1108 1 -0.03841026564975891 1108 2 0.03762390547563399 1108 3 -0.04916922404563451 1108 4 -0.015551903410404577 1108 5 -0.1978848769687218 1108 6 -0.04355875545360277 1108 7 -0.10158438865536823 1108 8 -0.07963877618191617 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5 27.0 6 73.0 7 183.0 8 483.0 9 931.0 10 1688.0 11 2967.0 12 4664.0 13 7106.0 14 9222.0 15 13179.0 16 19059.0 17 28154.0 18 25337.0 19 13282.0 20 9573.0 21 8879.0 22 8957.0 23 9335.0 24 10417.0 25 11946.0 26 14466.0 27 17492.0 28 21496.0 29 26452.0 30 32665.0 31 41081.0 32 51875.0 33 67997.0 34 93716.0 35 133923.0 36 200295.0 37 312475.0 38 269307.0 39 26286.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.715517881723585 19.991407618177035 15.541571000577509 19.751503499521863 2 15.253533251594803 21.84773222404384 40.95581905417418 21.942915470187177 3 19.632698354727825 27.59705446983333 30.60499220407074 22.165254971368103 4 12.093489613119903 17.775253827563812 38.15858211076708 31.97267444854921 5 12.063661968061975 39.84238377810633 33.354696709192716 14.739257544638976 6 28.87082436182744 37.93677772270571 17.70736135051849 15.485036564948352 7 26.36816719074308 36.17545288964429 20.140186259499092 17.316193660113537 8 26.555791228291962 34.53446161934595 19.538585334823626 19.371161817538464 9 27.785280509641737 16.611583459921032 20.84633490991036 34.75680112052687 10-11 24.37333237012972 28.57126134857871 26.11154700533976 20.943859275951816 12-13 26.392757962952988 26.356335059937248 27.64424757272624 19.606659404383525 14-15 23.3087917078871 26.34910378585843 28.504071207502825 21.838033298751647 16-17 19.834168444245563 30.005632065583466 28.890556658111542 21.26964283205943 18-19 19.521996828116983 27.312608402212422 33.86353010111631 19.301864668554277 20-21 21.403910811269093 25.795755812922927 34.06543570458917 18.73489767121881 22-23 20.507041443311934 26.25768726011802 33.7290954016238 19.50617589494624 24-25 19.93812052551065 26.38335842804329 34.418559695197935 19.259961351248126 26-27 20.083097629179086 26.39244382147421 34.279594792869645 19.244863756477056 28-29 19.648845035488748 26.587762653839746 34.51512092795828 19.248271382713224 30-31 19.821271201887622 26.71139345920826 34.35629706304839 19.111038275855726 32-33 19.614671731512342 26.73191033377012 34.07513389533589 19.57828403938165 34-35 20.012193890212178 26.6143526703148 34.08018402540182 19.2932694140712 36-37 20.177885038265618 26.448409559665297 33.98370780673447 19.389997595334616 38-39 20.173831496891996 26.574553632046854 34.03463252109851 19.21698234996264 40-41 20.171624155273772 26.751375073494444 33.877309263945214 19.199691507286566 42-43 19.8643335334659 26.967958162816487 33.475098547171356 19.692609756546258 44-45 20.090119738249417 27.368059499227765 32.85597898610779 19.68584177641502 46-47 20.352893724527778 27.635147835032654 31.836287492199055 20.175670948240516 48-49 20.59613608194187 27.821400645212645 31.42973529960649 20.152727973238992 50-51 21.016032657953687 28.267852209783072 30.83666274027415 19.879452391989087 52-53 20.7316604130874 28.543679124661665 30.586139696008825 20.13852076624211 54-55 20.458210675372957 28.576411812354557 30.569875405599024 20.39550210667346 56-57 20.895598761480684 28.981157998168577 29.93877637020731 20.18446687014343 58-59 20.818743139682585 28.486312140847126 30.310245208898127 20.384699510572162 60-61 20.843659344312517 28.34427305217152 30.60295208530238 20.209115518213576 62-63 21.042320089952515 28.191999925084165 30.65676439838904 20.10891558657428 64-65 20.914026719535844 28.18243477807187 30.71833585170945 20.185202650682836 66-67 20.7843286771803 28.441262305093407 30.390278064843667 20.38413095288262 68-69 20.776268535816794 28.744236664479395 30.252285769134808 20.227209030569007 70-71 20.88382627285017 28.567682779591586 29.899813446188688 20.648677501369555 72-73 20.80415569404599 28.482938898695593 29.885973930591724 20.826931476666697 74-75 20.776984769332646 28.71298519742343 29.685319830636587 20.824710202607342 76-77 20.756736906952526 28.461864160196814 30.111617907828105 20.66978102502256 78-79 20.646135714051603 28.59216420481187 29.911886935948967 20.84981314518756 80-81 20.516069781426356 29.024502160853665 29.73757383724936 20.721854220470618 82-83 20.566551394305055 29.07171189490371 29.474552009685556 20.887184701105678 84-85 20.60528124440847 28.739454484342055 29.39624048708786 21.259023784161617 86-87 20.55834371787231 28.763032512804255 29.50550196620627 21.17312180311717 88-89 20.848742918948425 28.35681476591142 29.61018347022196 21.184258844918197 90-91 20.514469711488772 28.557691375033155 29.718873890100234 21.20896502337784 92-93 20.29543275252607 28.617664893262994 29.756826188267976 21.330076165942955 94-95 20.216941547587275 28.82129295369733 29.517408233116683 21.444357265598715 96-97 20.158112549004656 28.65457177238161 29.323797617542613 21.863518061071122 98-99 20.335135778719284 28.584801666347825 29.18550159664911 21.89456095828378 100-101 20.477974268944816 28.72996775172204 29.413332878425564 21.378725100907577 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2220.0 1 1486.5 2 952.5 3 1205.0 4 1026.5 5 930.5 6 1755.0 7 3614.0 8 5486.0 9 6164.5 10 5320.5 11 4463.0 12 4564.5 13 4860.5 14 5098.0 15 5215.5 16 5221.0 17 5451.0 18 5769.5 19 6058.0 20 6443.0 21 6954.0 22 7823.5 23 9017.0 24 10218.5 25 11933.5 26 14404.5 27 16662.0 28 18527.5 29 20708.0 30 23342.0 31 26092.5 32 28902.0 33 31988.0 34 35553.5 35 38094.5 36 41498.5 37 45091.5 38 45778.0 39 46303.0 40 47356.0 41 49210.0 42 50470.5 43 50558.0 44 51565.0 45 52591.0 46 53341.5 47 55155.0 48 63591.0 49 61964.5 50 51658.5 51 46688.0 52 41366.5 53 37955.5 54 35165.5 55 31412.0 56 26976.5 57 23113.0 58 20522.5 59 18449.5 60 15694.0 61 12951.0 62 10193.0 63 7083.0 64 4747.5 65 3494.0 66 2579.5 67 1966.5 68 1459.0 69 1045.5 70 814.5 71 621.5 72 522.5 73 485.0 74 359.0 75 256.5 76 191.0 77 114.5 78 76.5 79 62.5 80 36.5 81 20.5 82 15.5 83 10.5 84 4.5 85 3.5 86 3.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04441438892422865 2 0.0 3 0.0 4 0.0 5 0.016120282727016724 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004849462646094243 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0033370991919123E-4 22-23 3.344456997306374E-4 24-25 5.351131195690199E-4 26-27 0.0015050056487878685 28-29 0.0020066741983838245 30-31 5.685576895420836E-4 32-33 0.0 34-35 0.0 36-37 3.344456997306374E-5 38-39 0.0 40-41 0.0 42-43 0.011270820080922481 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.344456997306374E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.3377827989225497E-4 74-75 6.688913994612748E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.688913994612748E-5 84-85 2.341119898114462E-4 86-87 0.0 88-89 0.0 90-91 0.007525028243939342 92-93 0.0034447907072255655 94-95 0.0 96-97 0.0 98-99 3.344456997306374E-4 100-101 0.013545050839090815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1495011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.53935078138234 #Duplication Level Percentage of deduplicated Percentage of total 1 71.31909013182475 36.75739603713139 2 15.449334583657514 15.924973488921324 3 5.774622348335394 8.928608605226032 4 2.6834886358928007 5.532210484925291 5 1.4917265006196705 3.8441307692660582 6 0.9148275287535914 2.8289770145337894 7 0.5426984537967792 1.9579228185122213 8 0.35701491079661635 1.472025337738459 9 0.2588858416972158 1.2008527386809602 >10 1.0990388652542482 9.809599112952691 >50 0.06750468373357367 2.4006562457280367 >100 0.03672448261574954 3.419525744037946 >500 0.002586170780551787 0.953820113110089 >1k 0.0020689366244414295 2.015404837937549 >5k 0.0 0.0 >10k+ 3.8792561708276803E-4 2.9538966512981046 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18559 1.24139554826018 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14987 1.0024675403726127 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10777 0.7208642611994159 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3719 0.24876071145964815 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3398 0.2272892975369412 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3058 0.20454698995525786 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3041 0.20340987457617368 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2579 0.17250709192106278 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1788 0.11959778222367595 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1677 0.1121730876896558 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.688913994612748E-5 0.0 8 0.0 0.0 0.0 6.688913994612748E-5 0.0 9 0.0 0.0 0.0 6.688913994612748E-5 0.0 10-11 0.0 0.0 0.0 6.688913994612748E-5 0.0 12-13 0.0 0.0 0.0 6.688913994612748E-5 6.688913994612748E-5 14-15 0.0 0.0 0.0 6.688913994612748E-5 6.688913994612748E-5 16-17 0.0 0.0 0.0 1.3377827989225497E-4 6.688913994612748E-5 18-19 0.0 0.0 0.0 2.0066741983838245E-4 6.688913994612748E-5 20-21 0.0 0.0 0.0 2.341119898114462E-4 6.688913994612748E-5 22-23 0.0 0.0 0.0 5.351131195690199E-4 6.688913994612748E-5 24-25 0.0 0.0 0.0 7.357805394074023E-4 6.688913994612748E-5 26-27 0.0 0.0 0.0 0.0012040045190302947 6.688913994612748E-5 28-29 0.0 0.0 0.0 0.0026755655978450994 6.688913994612748E-5 30-31 0.0 0.0 0.0 0.005217352915797944 6.688913994612748E-5 32-33 0.0 0.0 0.0 0.008361142493265935 6.688913994612748E-5 34-35 0.0 0.0 0.0 0.011404598360814738 6.688913994612748E-5 36-37 0.0 0.0 0.0 0.015417946757582388 6.688913994612748E-5 38-39 0.0 0.0 0.0 0.021036634513057095 1.0033370991919123E-4 40-41 0.0 0.0 0.0 0.02779243764761597 1.3377827989225497E-4 42-43 0.0 0.0 0.0 0.03364523739290212 1.3377827989225497E-4 44-45 0.0 0.0 0.0 0.036855916110316245 1.3377827989225497E-4 46-47 0.0 0.0 0.0 0.04183915703630274 1.3377827989225497E-4 48-49 0.0 0.0 0.0 0.05023374409954175 1.3377827989225497E-4 50-51 0.0 0.0 0.0 0.0606015607911915 2.0066741983838245E-4 52-53 0.0 0.0 0.0 0.08033385707529911 2.0066741983838245E-4 54-55 0.0 0.0 0.0 0.09397924162430912 2.0066741983838245E-4 56-57 0.0 0.0 0.0 0.12782514643704962 2.0066741983838245E-4 58-59 0.0 0.0 0.0 0.19377783842393131 2.0066741983838245E-4 60-61 0.0 0.0 0.0 0.25872719331162114 2.0066741983838245E-4 62-63 0.0 0.0 0.0 0.2915363164551966 2.0066741983838245E-4 64-65 0.0 0.0 0.0 0.34708774718045554 2.0066741983838245E-4 66-67 0.0 0.0 0.0 0.44491311435166697 2.0066741983838245E-4 68-69 0.0 0.0 0.0 0.5894605457752484 2.0066741983838245E-4 70-71 0.0 0.0 0.0 0.7295264048224395 2.0066741983838245E-4 72-73 0.0 0.0 0.0 0.8816323090599334 2.0066741983838245E-4 74-75 0.0 0.0 0.0 1.055677851199757 2.0066741983838245E-4 76-77 0.0 0.0 0.0 1.1907604693209615 2.0066741983838245E-4 78-79 0.0 0.0 0.0 1.3643043429111894 2.0066741983838245E-4 80-81 0.0 0.0 0.0 1.5883495171607434 2.0066741983838245E-4 82-83 0.0 0.0 0.0 1.787177485650607 2.0066741983838245E-4 84-85 0.0 0.0 0.0 2.050988253598134 2.0066741983838245E-4 86-87 0.0 0.0 0.0 2.376738365135775 2.341119898114462E-4 88-89 0.0 0.0 0.0 2.7691100600597585 2.6755655978450994E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13875 0.0 30.21697 1 GTATCAA 19640 0.0 27.978966 1 GAGTACT 13735 0.0 23.828718 12-13 AGTACTT 13930 0.0 23.068893 12-13 TATCAAC 23850 0.0 23.007866 2 ATCAACG 23935 0.0 22.886461 3 TACATGG 13390 0.0 22.67269 2 TCAACGC 24170 0.0 22.66394 4 GTACATG 13785 0.0 22.655512 1 GTACTTT 14545 0.0 22.336182 14-15 CAACGCA 24500 0.0 22.281105 5 AACGCAG 25015 0.0 21.949463 6 CATGGGG 2790 0.0 21.456017 4 ACATGGG 13450 0.0 21.335236 3 TACTTTT 15640 0.0 20.45349 14-15 ACTTTTT 16470 0.0 19.970676 16-17 CGCAGAG 27620 0.0 19.778088 8 ACGCAGA 27705 0.0 19.734554 7 GCAGAGT 28075 0.0 19.237597 9 GTATAGG 425 0.0 19.014078 1 >>END_MODULE