FastQCFastQC Report
Thu 26 May 2016
SRR937867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764067
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105780.5996370886139812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81330.46103691072958114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56610.3209061787335742No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24470.13871355226303764No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.11048333198228867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.041.8078771
GTATCAA94650.038.2494351
ATCAACG119150.030.2084373
TATCAAC121400.029.7277372
TCAACGC121200.029.5814744
CAACGCA124100.028.8918515
AACGCAG127650.028.1332656
GTACATG101800.027.0110261
TACATGG100500.026.6840522
ACATGGG101650.025.0249253
ACGCAGA145400.024.5695557
CGCAGAG145500.024.324128
CATGGGG36600.022.8656024
GCAGAGT151750.022.8514299
GAGTACT84350.021.51160212-13
TAGCCCT13500.021.1333624
AGTACTT86850.020.86504212-13
AGTACAT73800.020.5528982
GTACTTT88650.020.38606614-15
CATGGGA63900.020.3148144
Produced by FastQC (version 0.11.5)