FastQCFastQC Report
Thu 26 May 2016
SRR937867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764067
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129640.7348927223285737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111400.6314952890111316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76730.43496080364294554No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.1413211629717012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.13378176679230439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65050.043.9741971
GTATCAA110200.039.496871
ATCAACG140500.030.7058643
TATCAAC142600.030.3869512
TCAACGC142250.030.3281124
CAACGCA144250.029.8746815
AACGCAG148550.029.032026
CTCGCGT458.9547876E-426.38709478-79
ACGCAGA167300.025.668367
CGCAGAG167250.025.5340218
GTACATG98650.025.0469591
TACATGG98950.024.6327762
GCAGAGT176750.023.9734789
GAGTACT104300.023.77452312-13
AGTACTT106750.022.7616312-13
GTATAGG5250.022.6271291
TGCACCG2103.4389814E-822.6252045
ACATGGG100950.022.5915873
GTACTTT109600.022.4482914-15
AGAGTAC170650.020.29302810-11
Produced by FastQC (version 0.11.5)