##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937867_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1764067 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03505535787473 31.0 31.0 34.0 30.0 34.0 2 32.32670244384142 33.0 31.0 34.0 31.0 34.0 3 32.412265520527285 34.0 31.0 34.0 31.0 34.0 4 36.07165260729893 37.0 35.0 37.0 35.0 37.0 5 35.97846907175295 37.0 35.0 37.0 35.0 37.0 6 36.0213274212374 37.0 35.0 37.0 35.0 37.0 7 35.959333744126496 37.0 35.0 37.0 35.0 37.0 8 35.939689932411866 37.0 35.0 37.0 35.0 37.0 9 37.61696296115737 39.0 37.0 39.0 35.0 39.0 10-11 37.53363874501365 39.0 37.0 39.0 35.0 39.0 12-13 37.41928877984793 39.0 37.0 39.0 34.0 39.0 14-15 38.59438672113927 40.0 38.0 41.0 34.0 41.0 16-17 38.55252890054629 40.0 38.0 41.0 34.0 41.0 18-19 38.5683389576473 40.0 38.0 41.0 34.0 41.0 20-21 38.489961832515434 40.0 38.0 41.0 34.0 41.0 22-23 38.42592798346095 40.0 38.0 41.0 34.0 41.0 24-25 38.44425863643501 40.0 38.0 41.0 34.0 41.0 26-27 38.29443864660469 40.0 38.0 41.0 34.0 41.0 28-29 38.130890720137046 40.0 38.0 41.0 33.5 41.0 30-31 38.14687877501251 40.0 37.5 41.0 34.0 41.0 32-33 38.04109169322933 40.0 37.0 41.0 33.5 41.0 34-35 37.89392693134671 40.0 37.0 41.0 33.0 41.0 36-37 37.735562481470375 40.0 37.0 41.0 33.0 41.0 38-39 37.59696712199707 40.0 37.0 41.0 33.0 41.0 40-41 37.392169911913776 40.0 36.5 41.0 33.0 41.0 42-43 37.146311336247436 40.0 36.0 41.0 32.0 41.0 44-45 36.801679301296375 40.0 36.0 41.0 31.0 41.0 46-47 36.36434982344775 39.0 36.0 41.0 30.0 41.0 48-49 35.97421356445078 39.0 35.0 41.0 29.5 41.0 50-51 35.695746533436655 39.0 35.0 40.5 28.0 41.0 52-53 35.46216611954081 39.0 35.0 40.0 27.0 41.0 54-55 35.22804377611508 39.0 35.0 40.0 27.0 41.0 56-57 34.96580175242777 38.0 34.5 40.0 26.0 41.0 58-59 34.715974506637224 38.0 34.0 40.0 26.0 41.0 60-61 34.41070067066614 38.0 34.0 40.0 25.5 41.0 62-63 34.038560610226256 37.5 34.0 40.0 24.0 41.0 64-65 33.6338359030581 37.0 33.0 40.0 23.0 41.0 66-67 33.210047010686104 36.5 32.5 39.0 22.0 40.5 68-69 32.77615249307424 36.0 32.0 39.0 20.5 40.0 70-71 32.3801936094264 35.5 32.0 38.5 20.0 40.0 72-73 31.88759468886386 35.0 31.0 38.0 18.0 40.0 74-75 31.399144703687558 35.0 31.0 37.0 17.0 39.0 76-77 30.147117428079547 33.5 29.5 36.0 13.0 38.5 78-79 30.73255040766592 34.0 30.5 36.0 13.0 39.0 80-81 30.53964106805467 34.0 30.5 36.0 9.5 37.5 82-83 30.16119342405929 34.0 30.0 35.5 7.0 37.0 84-85 29.75684228546875 34.0 30.0 35.0 4.5 37.0 86-87 29.350550744387828 34.0 29.0 35.0 2.0 36.0 88-89 28.896177979634558 34.0 29.0 35.0 2.0 36.0 90-91 28.326429495024847 34.0 29.0 35.0 2.0 35.5 92-93 27.860071357833913 33.0 27.0 35.0 2.0 35.0 94-95 27.362582600320735 33.0 26.5 35.0 2.0 35.0 96-97 26.76517870352997 33.0 25.5 35.0 2.0 35.0 98-99 25.841002637654917 32.0 24.5 34.0 2.0 35.0 100-101 23.114996199123958 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2781008309055508 1101 2 0.1914124840274951 1101 3 0.20058713913127946 1101 4 0.06530496313965983 1101 5 -0.03117106798339364 1101 6 0.047375394627486855 1101 7 0.15095445973376798 1101 8 0.02338665506688642 1101 9 0.09614750500987412 1101 10-11 0.17004408391451165 1101 12-13 0.11862466658690352 1101 14-15 0.20683334994235736 1101 16-17 0.20296275071090975 1101 18-19 0.17040728099836144 1101 20-21 0.21057718263118375 1101 22-23 0.0949684686392871 1101 24-25 0.13389897369629722 1101 26-27 0.04632654443496165 1101 28-29 -0.05857926564478788 1101 30-31 0.07239886462187428 1101 32-33 0.09641670338127284 1101 34-35 0.19553535070281924 1101 36-37 0.12641551740072288 1101 38-39 0.03267176388843751 1101 40-41 0.18001887508361847 1101 42-43 0.16036049558911003 1101 44-45 0.23246237339184006 1101 46-47 0.20647233721124536 1101 48-49 0.27502148045161334 1101 50-51 0.4604297471839942 1101 52-53 0.4924713676301664 1101 54-55 0.4641622057677708 1101 56-57 0.381148028268143 1101 58-59 0.33171838566128997 1101 60-61 0.495105380759874 1101 62-63 0.32546195861888094 1101 64-65 0.31383344897880505 1101 66-67 0.30618686195465017 1101 68-69 0.39084344602206045 1101 70-71 0.27465911092308204 1101 72-73 0.07909735189107892 1101 74-75 -0.07235816893260605 1101 76-77 0.05776609360731655 1101 78-79 0.16390619165772335 1101 80-81 0.10074079026095006 1101 82-83 0.21343131597141607 1101 84-85 0.07209717015181027 1101 86-87 0.06026900939535551 1101 88-89 0.059255613529991535 1101 90-91 -0.02409280894233845 1101 92-93 -0.1456537568881906 1101 94-95 -0.22521367251747648 1101 96-97 -0.37543136002735267 1101 98-99 -0.407302529815464 1101 100-101 -0.3599704788020617 1102 1 0.2486461869462886 1102 2 0.1922052737789457 1102 3 0.16634308675597254 1102 4 0.056636003226998355 1102 5 -0.0068363430027531535 1102 6 0.04200010587315006 1102 7 0.053583298277722236 1102 8 0.036540240385029676 1102 9 0.06399119583820578 1102 10-11 0.10013470207000097 1102 12-13 0.11374868291640894 1102 14-15 0.19900972388211358 1102 16-17 0.12068774087590839 1102 18-19 0.11558792516726157 1102 20-21 0.12135484893802584 1102 22-23 0.1888276831185962 1102 24-25 0.11905002437413259 1102 26-27 0.06553781119700375 1102 28-29 0.05519382279128848 1102 30-31 0.12263592563701309 1102 32-33 0.1386131557015844 1102 34-35 0.08187810431318354 1102 36-37 0.1137071276323951 1102 38-39 0.10521951299988075 1102 40-41 0.13274483853960106 1102 42-43 0.2729464412672158 1102 44-45 0.21009213077694255 1102 46-47 0.093021796047843 1102 48-49 0.20763932012676634 1102 50-51 0.14502425237783712 1102 52-53 0.11433971879355198 1102 54-55 0.216780154342608 1102 56-57 0.14946849555671804 1102 58-59 0.13572861678726866 1102 60-61 0.1807647888083821 1102 62-63 0.14649361984283615 1102 64-65 0.06098497337030295 1102 66-67 0.09969412624467822 1102 68-69 0.13727323771056632 1102 70-71 0.08004399646269178 1102 72-73 0.038171418654755485 1102 74-75 -0.09749929612608454 1102 76-77 -0.14639782220777775 1102 78-79 -0.012227420095822339 1102 80-81 0.18895484893882752 1102 82-83 0.3446608207092332 1102 84-85 0.24762257713037528 1102 86-87 0.18840644352805924 1102 88-89 0.11316484019244655 1102 90-91 0.2500877549130358 1102 92-93 0.14550161438488374 1102 94-95 0.22174925122142852 1102 96-97 0.09697066768489648 1102 98-99 0.13682716427168984 1102 100-101 0.2978087706340453 1103 1 0.2792526652645222 1103 2 0.22586683387556405 1103 3 0.1809908947248573 1103 4 0.12868402122920486 1103 5 0.037331142952901075 1103 6 0.12819470305274905 1103 7 0.09843262577919631 1103 8 0.07405174876846843 1103 9 0.206960106021981 1103 10-11 0.15515627620670358 1103 12-13 0.29842238439306357 1103 14-15 0.24197468671167144 1103 16-17 0.0904108240378605 1103 18-19 0.10080266975640484 1103 20-21 0.06631836572962158 1103 22-23 0.0017537630891482081 1103 24-25 0.03379682857911348 1103 26-27 0.11656787186130657 1103 28-29 0.1541122477344814 1103 30-31 0.22220935579695578 1103 32-33 0.19559619570957665 1103 34-35 0.2920847980036072 1103 36-37 0.3040745325962888 1103 38-39 0.2972777486859002 1103 40-41 0.3680781457465656 1103 42-43 0.5265660798940459 1103 44-45 0.4717541486530621 1103 46-47 0.5713653044676335 1103 48-49 0.5427934060927555 1103 50-51 0.39467339222379394 1103 52-53 0.4733104078425683 1103 54-55 0.3366002458644317 1103 56-57 0.48135266712066027 1103 58-59 0.3797661240359247 1103 60-61 0.5185371917474839 1103 62-63 0.5114189921294567 1103 64-65 0.4049613960223155 1103 66-67 0.30448414119918255 1103 68-69 0.2732970763222795 1103 70-71 0.29950624186544417 1103 72-73 0.3763719249384323 1103 74-75 0.33390761475401476 1103 76-77 0.33180582492034105 1103 78-79 0.2605708223944099 1103 80-81 0.33207654160975153 1103 82-83 0.42160327396432606 1103 84-85 0.46682327481813246 1103 86-87 0.26922710131061933 1103 88-89 0.2700809739662944 1103 90-91 0.2251968467205394 1103 92-93 0.34352646056586167 1103 94-95 0.07692593650029522 1103 96-97 0.07210844170653274 1103 98-99 0.11656658777622653 1103 100-101 0.0763870472362278 1104 1 0.24605015965926924 1104 2 0.16625192043914438 1104 3 0.12894272423275055 1104 4 0.10099199802230885 1104 5 0.04588024957980963 1104 6 0.008019495600656512 1104 7 0.06799322028258104 1104 8 0.04958280301806184 1104 9 0.08703037391833846 1104 10-11 0.14908827352542886 1104 12-13 0.10635757150445357 1104 14-15 0.12283246356486188 1104 16-17 0.11161601091810525 1104 18-19 0.11091731737676724 1104 20-21 0.07372862391791557 1104 22-23 0.06705274836524922 1104 24-25 -0.03463462555027519 1104 26-27 0.14550923052806297 1104 28-29 0.15453616655656788 1104 30-31 0.17345476180209118 1104 32-33 0.16735312426284565 1104 34-35 0.32131416258766876 1104 36-37 0.25749338935610666 1104 38-39 0.09282798792034441 1104 40-41 0.10089047961152176 1104 42-43 0.11466699753729159 1104 44-45 0.11959918945004944 1104 46-47 0.14003391274722077 1104 48-49 0.23608513343482684 1104 50-51 0.2879244381521815 1104 52-53 0.3851077866651167 1104 54-55 0.23431492122212916 1104 56-57 0.3701835387661774 1104 58-59 0.34169641088611513 1104 60-61 0.39146163064445716 1104 62-63 0.38160436993496916 1104 64-65 0.24312009976454618 1104 66-67 0.27714322632966315 1104 68-69 0.4391362460516035 1104 70-71 0.2930189432500043 1104 72-73 0.199571194522143 1104 74-75 0.06386069244164716 1104 76-77 0.11549512062336831 1104 78-79 0.08134862328662607 1104 80-81 0.0930200730338342 1104 82-83 0.1288956485107029 1104 84-85 0.14052366545216088 1104 86-87 0.08609509474612054 1104 88-89 -4.677628295830516E-4 1104 90-91 -0.05440211775256287 1104 92-93 0.042330288317057096 1104 94-95 -0.09881476571637648 1104 96-97 -0.16005160267318885 1104 98-99 0.017619450499569922 1104 100-101 -0.09915648881073835 1105 1 0.23081720651985904 1105 2 0.10334759882658062 1105 3 0.0951104144984356 1105 4 0.034192150999743376 1105 5 -0.03267186398343824 1105 6 0.022261694876263505 1105 7 0.006905730687300604 1105 8 -0.045074235068824464 1105 9 0.06999228703615046 1105 10-11 -0.0060209478644139836 1105 12-13 0.06429552565621321 1105 14-15 -0.007261347597989243 1105 16-17 0.030175773353150248 1105 18-19 0.2076242531037451 1105 20-21 -0.1257310057867045 1105 22-23 -0.1604620432029904 1105 24-25 -0.1660309945858458 1105 26-27 -0.15255335524053493 1105 28-29 -0.049695009344993935 1105 30-31 -0.03901874835516139 1105 32-33 -0.03904133255476694 1105 34-35 -0.043811481379847805 1105 36-37 0.002875495495452185 1105 38-39 -0.01270349618574329 1105 40-41 0.08301191669955443 1105 42-43 0.1338832131454879 1105 44-45 -0.05300075205857979 1105 46-47 -0.06091364748764505 1105 48-49 -0.04207643773342795 1105 50-51 0.14560053092849756 1105 52-53 0.21380050448923527 1105 54-55 0.17878051254740512 1105 56-57 0.19566373043790009 1105 58-59 0.301524536237622 1105 60-61 0.158273175941531 1105 62-63 -0.0534198139978983 1105 64-65 0.2011340927005918 1105 66-67 0.19509782802591502 1105 68-69 0.37062260181413365 1105 70-71 0.015477830564122996 1105 72-73 0.16338663174515133 1105 74-75 0.14466157431157356 1105 76-77 0.10705661530289845 1105 78-79 0.2732831129494038 1105 80-81 0.15104351457620524 1105 82-83 0.23428855305728646 1105 84-85 0.3039958033118282 1105 86-87 0.09780641732604067 1105 88-89 0.07796466838681226 1105 90-91 0.28748400404446883 1105 92-93 0.38705594128324705 1105 94-95 0.09769239947675601 1105 96-97 0.1469376664096167 1105 98-99 0.20731821993107857 1105 100-101 0.040452968794262745 1106 1 0.07492326735286525 1106 2 0.08203196611054864 1106 3 0.07467965902297635 1106 4 -0.034259325351108316 1106 5 -0.05524084235349136 1106 6 -0.06595480508859453 1106 7 -0.05183099183340545 1106 8 -0.14458651562099334 1106 9 -0.05884284351204627 1106 10-11 -0.07667332591439191 1106 12-13 -0.30372514913180737 1106 14-15 -0.025616328546625766 1106 16-17 -0.022751983563701117 1106 18-19 -0.055838497366636375 1106 20-21 0.10201427400387075 1106 22-23 0.044553989436074914 1106 24-25 -0.05683437785867085 1106 26-27 -0.06387148819564459 1106 28-29 0.06744142190362368 1106 30-31 0.12252999420893218 1106 32-33 0.0839893259068063 1106 34-35 -0.30408729116350486 1106 36-37 0.01920078927354041 1106 38-39 0.0867310119527076 1106 40-41 -0.19643842244578025 1106 42-43 -0.13144042955305935 1106 44-45 -0.030894260308791388 1106 46-47 0.030474584336367627 1106 48-49 0.009358895368706044 1106 50-51 0.17438093274110855 1106 52-53 0.22129396296510606 1106 54-55 0.16337155063054354 1106 56-57 0.15622470573853775 1106 58-59 0.1807352211303055 1106 60-61 0.21481037938379188 1106 62-63 0.13495672431164252 1106 64-65 -0.15529676416706195 1106 66-67 -0.1614046946807477 1106 68-69 0.15418220486373002 1106 70-71 0.08959275169137015 1106 72-73 0.09737813093228453 1106 74-75 0.10471339954370862 1106 76-77 0.24054541665141826 1106 78-79 0.09528498640043637 1106 80-81 -0.0026223313514570634 1106 82-83 -0.14055028521872615 1106 84-85 -0.23724627398358322 1106 86-87 0.10691927028317849 1106 88-89 0.2620095712980337 1106 90-91 0.10910496549585602 1106 92-93 -0.07579039079689309 1106 94-95 0.07066262589241745 1106 96-97 0.06124548616657677 1106 98-99 0.06974965058631355 1106 100-101 -0.3114613271579998 1107 1 0.11549756277573664 1107 2 0.09553945145329124 1107 3 0.08506136179023827 1107 4 -0.0369427450922899 1107 5 0.06925957209582378 1107 6 0.03568629722429506 1107 7 0.09074488907742051 1107 8 0.11486460355165917 1107 9 0.050146426530794486 1107 10-11 0.012754016255918543 1107 12-13 -0.002397502358533643 1107 14-15 0.0382798459187228 1107 16-17 0.04380169094551434 1107 18-19 0.17378795593086238 1107 20-21 0.1681480494164731 1107 22-23 0.17187154268044225 1107 24-25 0.16196601601438942 1107 26-27 0.0954598155146229 1107 28-29 0.07614578795035953 1107 30-31 0.07820452043674209 1107 32-33 0.05773453342123247 1107 34-35 0.07119789196779891 1107 36-37 0.19461081230086563 1107 38-39 0.23770886244150802 1107 40-41 0.31912482719839375 1107 42-43 0.40648248021608424 1107 44-45 0.16624883077594887 1107 46-47 0.06074679698529195 1107 48-49 0.01116875484937907 1107 50-51 0.10231832563959387 1107 52-53 0.25879989791118163 1107 54-55 0.11635401721805749 1107 56-57 0.07887674004931 1107 58-59 0.27755074040950944 1107 60-61 0.20789606751055345 1107 62-63 0.18885135157044175 1107 64-65 0.3277065604661118 1107 66-67 0.16283623036799355 1107 68-69 0.35310325426321754 1107 70-71 0.298089068967613 1107 72-73 0.3807947351701415 1107 74-75 0.21967536537862387 1107 76-77 0.39415790468182976 1107 78-79 0.406813030981251 1107 80-81 0.3389111523781523 1107 82-83 0.4481897197472229 1107 84-85 0.5571415399837036 1107 86-87 0.4270105182514712 1107 88-89 0.40271099286715994 1107 90-91 0.6391122655459931 1107 92-93 0.6650902621561698 1107 94-95 0.519589523838988 1107 96-97 0.45063138340547937 1107 98-99 0.30228522437397487 1107 100-101 0.39905699235238146 1108 1 0.08933946595495712 1108 2 0.011315909136328628 1108 3 0.0059896883603869355 1108 4 -0.037402041033367084 1108 5 0.0018447700016750446 1108 6 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-0.6294570576495744 2316 62-63 -0.4994728177461667 2316 64-65 -0.44198817964289105 2316 66-67 -0.38301052187676277 2316 68-69 -0.24514589806120313 2316 70-71 -0.36332967938272276 2316 72-73 -0.2875975769175625 2316 74-75 -0.2756257999517082 2316 76-77 -0.23785493686591863 2316 78-79 -0.184417599619767 2316 80-81 -0.44024579353401094 2316 82-83 -0.41145289810312846 2316 84-85 -0.2718332052302017 2316 86-87 -0.4419543555422756 2316 88-89 -0.266163288167629 2316 90-91 -0.11876674236178175 2316 92-93 0.058100407191599146 2316 94-95 0.10986830202595854 2316 96-97 0.18950283160372905 2316 98-99 0.3676816909663252 2316 100-101 0.2971771200910709 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 13.0 5 45.0 6 74.0 7 209.0 8 529.0 9 987.0 10 1678.0 11 2685.0 12 4237.0 13 6193.0 14 8243.0 15 11701.0 16 16092.0 17 22911.0 18 20416.0 19 11808.0 20 9556.0 21 9349.0 22 9869.0 23 10800.0 24 12343.0 25 14749.0 26 17661.0 27 22121.0 28 27031.0 29 33870.0 30 41836.0 31 52435.0 32 66510.0 33 85618.0 34 117506.0 35 165959.0 36 250927.0 37 381295.0 38 301887.0 39 24918.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.765808943806235 20.35101187786365 15.286094411694497 21.597084766635614 2 15.372942184168743 21.238875847686057 40.727988222669545 22.660193745475652 3 19.209191034127386 25.790290278090346 30.702235232562032 24.298283455220236 4 12.349020757148113 17.551204120931914 37.87843658999346 32.221338531926506 5 12.608110062295669 38.838584184215975 34.42594131107287 14.127364442415496 6 30.482288938005187 37.483043444495024 17.59122527659097 14.443442340908822 7 27.300493688731777 34.513371657652456 20.705846206521635 17.480288447094132 8 26.098215090469917 35.96484714015965 19.607985410984956 18.328952358385482 9 26.554320215728765 16.63559263905509 20.98100582347496 35.82908132174118 10-11 24.44524499352916 27.939046532813094 27.140805876420792 20.47490259723695 12-13 25.373265899107732 25.40416175710697 28.32234640719437 20.900225936590928 14-15 22.32500239503375 26.374253358857686 27.45323732035121 23.847506925757354 16-17 20.584507277784798 29.806832733677346 28.22528849527824 21.38337149325961 18-19 20.442874335271846 27.765527046308332 31.61438879589041 20.177209822529417 20-21 21.65033981135637 26.73299823646154 31.773282987550928 19.84337896463116 22-23 21.160166264421655 26.83098481767176 31.709028758983198 20.29982015892339 24-25 20.67475519567906 26.92260816229815 32.02574512687196 20.376891515150827 26-27 20.72379003399852 27.203519179613927 31.792652975614317 20.280037810773237 28-29 20.404223465447956 27.2705860211366 32.04862790092565 20.27656261248979 30-31 20.670189964440127 27.108437491319776 32.009016664333046 20.212355879907058 32-33 20.505712084631707 27.098602263972964 31.84249804570915 20.55318760568618 34-35 20.849860010985978 27.21761701794773 31.645708467989024 20.286814503077263 36-37 20.921569305474225 27.03508426834128 31.641655333952734 20.401691092231758 38-39 20.70267172391978 27.337793859303527 31.676744704141058 20.282789712635633 40-41 20.84481612650417 27.234563597171853 31.6148187199644 20.305801556359576 42-43 20.6757730127904 27.263450106485116 31.38644955967348 20.674327321051 44-45 20.8736402869052 27.575511587711805 31.008175993315447 20.542672132067548 46-47 20.988743624816973 27.774483622220696 30.418799285968163 20.817973466994168 48-49 21.066263356210392 27.8529953794272 30.24193525529359 20.838806009068815 50-51 21.256023722454987 28.21196700578833 29.69172939576558 20.840279875991104 52-53 21.285416030116767 28.389482939140066 29.567159297237577 20.757941733505586 54-55 21.172144685103813 28.25501660794341 29.60734734622949 20.965491360723284 56-57 21.21248796105817 28.66200660179007 29.395028646871125 20.73047679028064 58-59 21.327988109295166 28.242322995668534 29.51758635017831 20.91210254485799 60-61 21.36307747948349 28.271828677708953 29.694365350068903 20.670728492738654 62-63 21.291453215779217 28.165143387411025 29.795381921434956 20.7480214753748 64-65 21.28266670143481 28.042387278941224 29.637904909507405 21.037041110116565 66-67 21.093529894272724 28.360033944288965 29.53535778402975 21.01107837740857 68-69 21.22113275742928 28.351474178701828 29.450327000051583 20.977066063817304 70-71 21.13295583444393 28.397220740482076 29.376917089883776 21.092906335190218 72-73 21.21880858266721 28.240310600447714 29.25129827835337 21.2895825385317 74-75 21.216263672599645 28.454426179511938 28.947803271588686 21.38150687629973 76-77 21.074029501147066 28.445008041077806 29.221622534744995 21.259339923030137 78-79 21.120569683577777 28.414623707602942 29.070267739263873 21.394538869555408 80-81 20.949771182160315 28.666654951314207 29.02367086964384 21.359902996881637 82-83 21.046142334199235 28.682252937483437 28.906821083839624 21.3647836444777 84-85 21.121879475632014 28.431666266549477 28.846673297180125 21.599780960638387 86-87 21.012240464789603 28.327212061673396 29.011794903481558 21.648752570055446 88-89 21.19074842395442 28.135949484911855 29.05000206908241 21.623300022051318 90-91 20.988422145640637 28.384409823671795 29.14544759265501 21.481720438032557 92-93 20.909881453397674 28.260995265392015 29.139579100605882 21.689544180604432 94-95 20.689690357565784 28.39478319134137 29.07091964194104 21.844606809151806 96-97 20.920690654039785 28.19909901381297 28.937903152204537 21.942307179942713 98-99 20.943904224692616 28.240654790366804 28.73395272400854 22.081488260932044 100-101 20.918514587475812 28.101635219269973 29.027906056394965 21.951944136859254 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 962.0 1 675.0 2 504.5 3 676.0 4 613.5 5 553.5 6 1059.5 7 2257.5 8 3450.5 9 4053.5 10 3749.0 11 3268.5 12 3344.5 13 3554.0 14 3843.0 15 4048.0 16 4148.0 17 4334.0 18 4577.5 19 4857.5 20 5280.5 21 5818.0 22 6574.0 23 7839.0 24 9396.5 25 11054.0 26 13551.0 27 16774.5 28 18959.0 29 21467.5 30 25206.0 31 28670.0 32 32616.5 33 37266.0 34 42155.0 35 46823.0 36 52168.5 37 56455.0 38 58413.0 39 59366.0 40 60021.5 41 61667.5 42 62573.0 43 63888.0 44 66817.5 45 69084.5 46 69105.5 47 68002.5 48 69526.5 49 67587.5 50 62079.5 51 56957.0 52 51207.0 53 47481.0 54 44553.5 55 39993.5 56 34681.0 57 30135.0 58 26145.0 59 22450.0 60 18455.5 61 14854.5 62 11786.0 63 8913.0 64 6616.0 65 4923.5 66 3690.5 67 3058.0 68 2396.5 69 1528.5 70 984.5 71 717.5 72 583.5 73 457.0 74 375.0 75 299.0 76 198.0 77 119.0 78 77.0 79 57.0 80 37.0 81 25.5 82 17.5 83 19.0 84 13.5 85 3.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020747511290670935 2 0.0 3 0.0 4 0.0 5 0.03979440690177867 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004336569982886137 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.385282985283439E-4 24-25 2.834359465938652E-4 26-27 0.0011054001917160743 28-29 0.0010203694077379147 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.219642451222091E-4 42-43 0.01164921740500786 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 8.503078397815956E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.8343594659386517E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.219642451222091E-4 84-85 2.8343594659386517E-5 86-87 0.0 88-89 0.0 90-91 0.01278296119138332 92-93 0.0019840516261570565 94-95 0.0 96-97 0.0 98-99 0.0012187745703536204 100-101 0.018593398096557554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1764067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.63851609183078 #Duplication Level Percentage of deduplicated Percentage of total 1 74.23582809216015 40.56135487803876 2 14.264854221362391 15.588209338430584 3 4.984788811258286 8.170843910349419 4 2.261164598570821 4.941867132211596 5 1.247425011694247 3.4078725787404154 6 0.7310882919224571 2.396734764165255 7 0.4702352542338668 1.7985069553782287 8 0.319718506075222 1.3975155791237692 9 0.21938725950654855 1.0788294878002087 >10 1.142658383214052 11.39001611729043 >50 0.08542855014269914 3.2193999784835605 >100 0.035459131499350205 3.3349065887814824 >500 0.0011369879978451159 0.45993859696563955 >1k 5.168127262932344E-4 0.45937383935639886 >5k 1.0336254525864689E-4 0.43333851357040176 >10k+ 2.0672509051729377E-4 1.3612917413138232 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12964 0.7348927223285737 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11140 0.6314952890111316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7673 0.43496080364294554 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2493 0.1413211629717012 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2360 0.13378176679230439 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.802462718252765E-4 0.0 0.0 0.0 0.0 7 6.802462718252765E-4 0.0 0.0 0.0 0.0 8 6.802462718252765E-4 0.0 0.0 0.0 0.0 9 6.802462718252765E-4 0.0 0.0 5.668718931877304E-5 0.0 10-11 6.802462718252765E-4 0.0 0.0 5.668718931877304E-5 0.0 12-13 6.802462718252765E-4 0.0 0.0 5.668718931877304E-5 0.0 14-15 7.369334611440495E-4 0.0 0.0 5.668718931877304E-5 0.0 16-17 0.0011904309756942339 0.0 0.0 5.668718931877304E-5 0.0 18-19 0.0011904309756942339 0.0 0.0 5.668718931877304E-5 0.0 20-21 0.0011904309756942339 0.0 0.0 5.668718931877304E-5 0.0 22-23 0.0011904309756942339 0.0 0.0 1.7006156795631912E-4 0.0 24-25 0.0011904309756942339 0.0 0.0 2.834359465938652E-4 0.0 26-27 0.0011904309756942339 0.0 0.0 5.101847038689574E-4 0.0 28-29 0.0011904309756942339 0.0 0.0 0.0010770565970566878 0.0 30-31 0.0011904309756942339 0.0 0.0 0.0026926414926417195 0.0 32-33 0.0012187745703536204 0.0 0.0 0.004875098281414482 0.0 34-35 0.001247118165013007 0.0 0.0 0.006348965203702581 0.0 36-37 0.00130380535433178 0.0 0.0 0.009551791400213257 0.0 38-39 0.00130380535433178 0.0 0.0 0.013973392167077554 0.0 40-41 0.0013888361383099395 0.0 0.0 0.01995389064020811 0.0 42-43 0.001473866922288099 0.0 0.0 0.029193902499168117 0.0 44-45 0.001473866922288099 0.0 0.0 0.038348883574149964 0.0 46-47 0.001473866922288099 0.0 0.0 0.05563847631637574 0.0 48-49 0.001473866922288099 0.0 0.0 0.07734966982546582 0.0 50-51 0.0015022105169474856 0.0 0.0 0.0889422000411549 0.0 52-53 0.0015305541116068721 0.0 0.0 0.10954799335852891 0.0 54-55 0.0015305541116068721 0.0 0.0 0.14202975283818586 0.0 56-57 0.0015305541116068721 0.0 0.0 0.19137595114017777 0.0 58-59 0.0015305541116068721 0.0 0.0 0.25849358329360506 0.0 60-61 0.0017006156795631912 0.0 0.0 0.3351630068472456 0.0 62-63 0.0019273644368382834 0.0 0.0 0.4176712109007198 0.0 64-65 0.0019273644368382834 0.0 0.0 0.506273287805962 0.0 66-67 0.00195570803149767 0.0 0.0 0.6165298710309757 0.0 68-69 0.0019840516261570565 0.0 0.0 0.7556118900245852 0.0 70-71 0.002069082410135216 0.0 0.0 0.8616452776453503 0.0 72-73 0.002437549140707241 0.0 0.0 0.9815670266492146 0.0 74-75 0.002437549140707241 0.0 0.0 1.1132230238420648 0.0 76-77 0.0024658927353666273 0.0 0.0 1.2638692294567042 0.0 78-79 0.002494236330026014 0.0 0.0 1.4373887159614687 0.0 80-81 0.002494236330026014 0.0 0.0 1.665044468265661 0.0 82-83 0.002494236330026014 0.0 0.0 1.9058799920864684 0.0 84-85 0.002494236330026014 0.0 0.0 2.2055851620148212 0.0 86-87 0.002494236330026014 0.0 0.0 2.5759792570236844 0.0 88-89 0.002550923519344787 0.0 0.0 3.0171473078970354 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6505 0.0 43.974197 1 GTATCAA 11020 0.0 39.49687 1 ATCAACG 14050 0.0 30.705864 3 TATCAAC 14260 0.0 30.386951 2 TCAACGC 14225 0.0 30.328112 4 CAACGCA 14425 0.0 29.874681 5 AACGCAG 14855 0.0 29.03202 6 CTCGCGT 45 8.9547876E-4 26.387094 78-79 ACGCAGA 16730 0.0 25.66836 7 CGCAGAG 16725 0.0 25.534021 8 GTACATG 9865 0.0 25.046959 1 TACATGG 9895 0.0 24.632776 2 GCAGAGT 17675 0.0 23.973478 9 GAGTACT 10430 0.0 23.774523 12-13 AGTACTT 10675 0.0 22.76163 12-13 GTATAGG 525 0.0 22.627129 1 TGCACCG 210 3.4389814E-8 22.625204 5 ACATGGG 10095 0.0 22.591587 3 GTACTTT 10960 0.0 22.44829 14-15 AGAGTAC 17065 0.0 20.293028 10-11 >>END_MODULE