FastQCFastQC Report
Thu 26 May 2016
SRR937866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538400
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94500.6142745709828393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73510.4778341133645346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49530.3219578783151326No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21440.1393655746229849No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.11069942797711908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.045.016711
GTATCAA83750.038.50551
ATCAACG105250.030.6659583
TCAACGC106100.030.466084
CAACGCA107550.030.0101575
TATCAAC107550.029.9679132
AACGCAG110300.029.3337966
GTACATG90250.026.2985151
TACATGG89850.025.7660662
CGCAGAG127350.025.1826538
ACATGGG89800.025.1435263
ACGCAGA128450.025.0031727
GAGTACT74150.023.76778212-13
GCAGAGT132050.023.4228239
CATGGGA56150.022.0113494
GTACTTT80500.021.71554614-15
AGTACTT76400.021.5444712-13
CAGAGTA130000.020.37170810-11
CATGGGG28050.020.1668074
ACCGTCC1908.23731E-620.0047458
Produced by FastQC (version 0.11.5)