FastQCFastQC Report
Thu 26 May 2016
SRR937866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538400
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114880.7467498699947998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98190.6382605304212169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68050.44234269370774826No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23540.15301612064482578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.13078523140925638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60850.042.246941
GTATCAA95150.033.9592971
GTACATG84700.027.3775771
TACATGG84700.026.9795992
ATCAACG119300.026.8008443
TATCAAC120900.026.6819342
TCAACGC120550.026.5229434
CAACGCA122350.026.132745
AACGCAG125700.025.3927086
ACATGGG87500.025.030343
ACGCAGA142700.022.3018047
CGCAGAG142650.022.2097268
GAGTACT90650.022.08573512-13
CATGGGA54850.021.9138014
AGTACTT92500.021.66969712-13
CTATACG1100.002874719721.59494
GTACTTT95900.020.82611814-15
GTATAGG4800.020.7891451
GCAGAGT151250.020.6956569
CATGGGG29750.019.482594
Produced by FastQC (version 0.11.5)