##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937866_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1538400 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1389359074363 33.0 31.0 34.0 31.0 34.0 2 32.35907696307852 34.0 31.0 34.0 31.0 34.0 3 32.42617134685388 34.0 31.0 34.0 31.0 34.0 4 36.06407176287051 37.0 35.0 37.0 35.0 37.0 5 35.972121684867396 37.0 35.0 37.0 35.0 37.0 6 36.01290886635466 37.0 35.0 37.0 35.0 37.0 7 35.952183437337496 37.0 35.0 37.0 35.0 37.0 8 35.935517420696826 37.0 35.0 37.0 35.0 37.0 9 37.604108164326576 39.0 37.0 39.0 35.0 39.0 10-11 37.52683989859594 39.0 37.0 39.0 35.0 39.0 12-13 37.418656721268846 39.0 37.0 39.0 34.0 39.0 14-15 38.59761375455018 40.0 38.0 41.0 34.0 41.0 16-17 38.565635400416014 40.0 38.0 41.0 34.0 41.0 18-19 38.587614404576186 40.0 38.0 41.0 34.0 41.0 20-21 38.50232319292772 40.0 38.0 41.0 34.0 41.0 22-23 38.340758255330215 40.0 38.0 41.0 34.0 41.0 24-25 38.40754485179407 40.0 38.0 41.0 34.0 41.0 26-27 38.26603126625065 40.0 38.0 41.0 34.0 41.0 28-29 38.11022035881435 40.0 37.5 41.0 33.0 41.0 30-31 38.129828393135725 40.0 37.0 41.0 34.0 41.0 32-33 38.025278211128445 40.0 37.0 41.0 33.5 41.0 34-35 37.881545436817476 40.0 37.0 41.0 33.0 41.0 36-37 37.70724583983359 40.0 37.0 41.0 33.0 41.0 38-39 37.54825955538222 40.0 37.0 41.0 33.0 41.0 40-41 37.354141640665624 40.0 36.0 41.0 33.0 41.0 42-43 37.112555902236096 40.0 36.0 41.0 31.5 41.0 44-45 36.74592758710348 40.0 36.0 41.0 31.0 41.0 46-47 36.32134717888715 39.0 36.0 41.0 30.0 41.0 48-49 35.93155356214248 39.0 35.0 41.0 29.0 41.0 50-51 35.66727021580863 39.0 35.0 41.0 28.0 41.0 52-53 35.41054504680187 39.0 35.0 40.0 27.0 41.0 54-55 35.192252990119606 39.0 35.0 40.0 26.5 41.0 56-57 34.940408216328656 38.0 34.5 40.0 26.0 41.0 58-59 34.68361706968278 38.0 34.0 40.0 26.0 41.0 60-61 34.35005427717108 38.0 34.0 40.0 25.0 41.0 62-63 34.00171899375975 37.5 34.0 40.0 23.5 41.0 64-65 33.6239372074883 37.0 33.0 40.0 22.5 41.0 66-67 33.20113917056682 36.5 33.0 39.0 22.0 40.5 68-69 32.75939580083204 36.0 32.0 39.0 20.5 40.0 70-71 32.336646840873634 35.5 31.5 38.5 19.5 40.0 72-73 31.832698257930318 35.0 31.0 38.0 18.0 40.0 74-75 31.314292121684865 35.0 31.0 37.0 14.5 39.0 76-77 30.057558827353095 33.5 29.5 36.0 13.0 38.5 78-79 30.6609392875715 34.0 30.5 36.0 11.0 39.0 80-81 30.461322802912115 34.0 30.5 36.0 7.5 37.5 82-83 30.083975559022363 34.0 30.0 35.5 7.0 37.0 84-85 29.679534906396256 34.0 30.0 35.0 2.0 37.0 86-87 29.230133255330216 34.0 29.0 35.0 2.0 36.0 88-89 28.832578328133124 34.0 29.0 35.0 2.0 36.0 90-91 28.278379160166406 34.0 28.5 35.0 2.0 35.5 92-93 27.779011310452418 33.0 27.0 35.0 2.0 35.0 94-95 27.268411336453457 33.0 26.0 35.0 2.0 35.0 96-97 26.68846236349454 33.0 25.5 35.0 2.0 35.0 98-99 25.7777483099324 32.0 24.0 34.0 2.0 35.0 100-101 23.146206448257928 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24673708331308575 1101 2 0.19221971426647144 1101 3 0.1534293180760571 1101 4 0.14408923584543487 1101 5 0.019926084606879613 1101 6 0.07973904981793822 1101 7 0.13516583235676904 1101 8 0.09497556869983015 1101 9 0.14792558014670476 1101 10-11 0.19800741291495427 1101 12-13 0.18214567852558616 1101 14-15 0.26785201647926016 1101 16-17 0.3034537957546135 1101 18-19 0.27446030970689606 1101 20-21 0.2356609081342853 1101 22-23 0.24685489013285178 1101 24-25 0.2725439468469659 1101 26-27 0.13179032160465454 1101 28-29 0.19317970099239545 1101 30-31 0.31277737990671284 1101 32-33 0.3666300044411699 1101 34-35 0.3245831406587101 1101 36-37 0.3667160443748969 1101 38-39 0.3572097294595551 1101 40-41 0.31741175833987256 1101 42-43 0.5897815048472239 1101 44-45 0.6609448117346091 1101 46-47 0.6067974082998973 1101 48-49 0.6707837412595481 1101 50-51 0.7066643856235615 1101 52-53 0.613927722324469 1101 54-55 0.7006012163280317 1101 56-57 0.6644798520223532 1101 58-59 0.6823901591138863 1101 60-61 0.7178339819865656 1101 62-63 0.7292781802421402 1101 64-65 0.7922614103790053 1101 66-67 0.851672262430192 1101 68-69 0.8870628880046212 1101 70-71 0.8256095964573902 1101 72-73 0.7676458581408419 1101 74-75 0.788280515274149 1101 76-77 0.7467141823815915 1101 78-79 0.7547520785498634 1101 80-81 0.8231702258950477 1101 82-83 0.8586657705798437 1101 84-85 0.84414516024761 1101 86-87 0.7621418807100397 1101 88-89 0.9350264193535658 1101 90-91 0.9729809124715452 1101 92-93 1.021139486299436 1101 94-95 0.9498217504654285 1101 96-97 0.9199797142549961 1101 98-99 0.8044194274933858 1101 100-101 1.1848212089626067 1102 1 0.20528906210591913 1102 2 0.1732098319579265 1102 3 0.18421671049480892 1102 4 0.1266229671697232 1102 5 0.0301104790391733 1102 6 0.04672625936709096 1102 7 0.08688543193047593 1102 8 0.16080752941413579 1102 9 0.18135975504222301 1102 10-11 0.2448755652463248 1102 12-13 0.1478875208353827 1102 14-15 0.22465428309700286 1102 16-17 0.22372444441514716 1102 18-19 0.217015723201186 1102 20-21 0.22838287338468177 1102 22-23 0.2483422826064583 1102 24-25 0.1358941684680417 1102 26-27 0.34417315533757176 1102 28-29 0.23652114114264577 1102 30-31 0.29345087648435 1102 32-33 0.25383856629294854 1102 34-35 0.2772224361225639 1102 36-37 0.3010492504564368 1102 38-39 0.34281889531856535 1102 40-41 0.40920082258393364 1102 42-43 0.5086632166252016 1102 44-45 0.4061555231544105 1102 46-47 0.4532390477198689 1102 48-49 0.3455838956071986 1102 50-51 0.3963744599616419 1102 52-53 0.3732900537089918 1102 54-55 0.4325956358980463 1102 56-57 0.22554926348828985 1102 58-59 0.3634794728701891 1102 60-61 0.35110322319837195 1102 62-63 0.29065248865332194 1102 64-65 0.44174065160726883 1102 66-67 0.4074136220607656 1102 68-69 0.34524757807793804 1102 70-71 0.3579260672824489 1102 72-73 0.2533501200338115 1102 74-75 0.1766278984122991 1102 76-77 0.1893330846483714 1102 78-79 0.4493953539232969 1102 80-81 0.37490411387068434 1102 82-83 0.40226096013291013 1102 84-85 0.4595931167318845 1102 86-87 0.4746832992494241 1102 88-89 0.4478059687834808 1102 90-91 0.4136573736868989 1102 92-93 0.46246790727469644 1102 94-95 0.3633516878213463 1102 96-97 0.6191375487146757 1102 98-99 0.6341022935328979 1102 100-101 0.9687107066855098 1103 1 0.20847505286243972 1103 2 0.20314458523735368 1103 3 0.18209328639796496 1103 4 0.12403919247715578 1103 5 0.07266100365653472 1103 6 0.08883067690963742 1103 7 0.10491829913983963 1103 8 -0.028373775793752998 1103 9 0.09666891920962684 1103 10-11 0.06163010494130816 1103 12-13 0.10798429701383583 1103 14-15 0.10794543661708644 1103 16-17 0.210238963690621 1103 18-19 0.06446789915538886 1103 20-21 0.054389719390563585 1103 22-23 0.18069894720686364 1103 24-25 0.14799546383081008 1103 26-27 0.11666025640503364 1103 28-29 0.07058611666658265 1103 30-31 0.15651110264867896 1103 32-33 0.2383236687095618 1103 34-35 0.21546195972806714 1103 36-37 0.13335543601874633 1103 38-39 0.26126619201224344 1103 40-41 0.22981279261100696 1103 42-43 0.36612773316591785 1103 44-45 0.3813826218517562 1103 46-47 0.4810352787702854 1103 48-49 0.3485120786353022 1103 50-51 0.4687685793246317 1103 52-53 0.5006009225409755 1103 54-55 0.57100961414762 1103 56-57 0.40663711524627644 1103 58-59 0.4091299573777931 1103 60-61 0.37359545193336885 1103 62-63 0.6400546026197205 1103 64-65 0.48769423823978997 1103 66-67 0.47110409553818755 1103 68-69 0.4647706594186971 1103 70-71 0.5903851712852202 1103 72-73 0.48742443930746404 1103 74-75 0.5506191722616514 1103 76-77 0.5310550921416031 1103 78-79 0.5115287256951433 1103 80-81 0.666932186141171 1103 82-83 0.6688241808550721 1103 84-85 0.6383840583083789 1103 86-87 0.7643141869512213 1103 88-89 0.7902153685956925 1103 90-91 0.7113629133909996 1103 92-93 0.557817739542994 1103 94-95 0.8256784296330331 1103 96-97 1.0707921900514243 1103 98-99 0.9585748274666628 1103 100-101 1.297580215523329 1104 1 0.11416191737602333 1104 2 0.05125845894689007 1104 3 -0.05886552667645617 1104 4 -0.025746077100308185 1104 5 -0.039771858575598174 1104 6 -0.010001346798730992 1104 7 -0.01770686878577976 1104 8 0.02911200412169279 1104 9 -0.044050153243290424 1104 10-11 0.016462578833255748 1104 12-13 0.07335353524864274 1104 14-15 0.1544198268229664 1104 16-17 0.07427044860784804 1104 18-19 0.02571207859999447 1104 20-21 0.0464419978521704 1104 22-23 0.04802122213456528 1104 24-25 -0.015250327309999534 1104 26-27 0.07424084771109563 1104 28-29 -0.10703071344335768 1104 30-31 0.09952333426000592 1104 32-33 0.15203190492537288 1104 34-35 0.16534688033205214 1104 36-37 0.2530502693663763 1104 38-39 0.11152460908102313 1104 40-41 0.2156344458566153 1104 42-43 0.3280800747701562 1104 44-45 0.5082881066858391 1104 46-47 0.6110844968930067 1104 48-49 0.5268163644089796 1104 50-51 0.4929945299070795 1104 52-53 0.4628397446060788 1104 54-55 0.35361674224920847 1104 56-57 0.5629351867975387 1104 58-59 0.5876363547918046 1104 60-61 0.5318648630958478 1104 62-63 0.6288953441688747 1104 64-65 0.5733124318927807 1104 66-67 0.5422544575039794 1104 68-69 0.5895879550431431 1104 70-71 0.7410887536551698 1104 72-73 0.6315251667469646 1104 74-75 0.6387188161424042 1104 76-77 0.4123461316044512 1104 78-79 0.5894540907141241 1104 80-81 0.6465285734991291 1104 82-83 0.638511576104559 1104 84-85 0.6395457333291041 1104 86-87 0.4938906798327345 1104 88-89 0.41584427765731036 1104 90-91 0.34374254612434996 1104 92-93 0.4491616609603035 1104 94-95 0.4512035626093329 1104 96-97 0.5252253939813372 1104 98-99 0.55860344303197 1104 100-101 0.6901460638043169 1105 1 0.11811819389685496 1105 2 0.12677648271028374 1105 3 0.038785010740717496 1105 4 0.023327752957705172 1105 5 -0.07388818528712449 1105 6 -0.006165079275561425 1105 7 -0.03595688029418653 1105 8 -0.006696107722675038 1105 9 0.13421466942582327 1105 10-11 0.10153006228478034 1105 12-13 0.08970249970367661 1105 14-15 0.05169423553742547 1105 16-17 0.07783021298816806 1105 18-19 0.12390396313726626 1105 20-21 0.2020428097321414 1105 22-23 -0.001101656827017905 1105 24-25 0.10070932152397916 1105 26-27 -0.06314191485581944 1105 28-29 9.896466760963563E-4 1105 30-31 0.08483348917212652 1105 32-33 0.06881320330210627 1105 34-35 0.10982281792496451 1105 36-37 0.10831811155836135 1105 38-39 0.1218303063725017 1105 40-41 -0.02188054772984316 1105 42-43 0.08432350769487584 1105 44-45 0.1122710786754908 1105 46-47 0.18924744257717663 1105 48-49 0.3410099712864394 1105 50-51 0.3774706926424969 1105 52-53 0.229283942331854 1105 54-55 0.2988715322063271 1105 56-57 0.25389081020407644 1105 58-59 0.5291179975360691 1105 60-61 0.48859810158789685 1105 62-63 0.38531889110013395 1105 64-65 0.38947704081723344 1105 66-67 0.4674503566135968 1105 68-69 0.30316266124725644 1105 70-71 0.5299021661984682 1105 72-73 0.43515627315956706 1105 74-75 0.3183917845880764 1105 76-77 0.3199884533302786 1105 78-79 0.6670549842082529 1105 80-81 0.47475489076874666 1105 82-83 0.48744286281208815 1105 84-85 0.602754030152159 1105 86-87 0.6668774183359467 1105 88-89 0.6809526968413024 1105 90-91 0.45650145247049423 1105 92-93 0.5986061318723905 1105 94-95 0.7277049967872138 1105 96-97 1.1390115130210177 1105 98-99 1.005284269554238 1105 100-101 0.9374183718587723 1106 1 0.02334160751055947 1106 2 0.0015413197664173595 1106 3 0.10986493137731657 1106 4 0.12851423578665333 1106 5 0.016570320734153654 1106 6 0.03664843184952815 1106 7 -0.014014300862768891 1106 8 0.07727860199531023 1106 9 0.05163355521447244 1106 10-11 -0.10756959742801797 1106 12-13 -0.2178150562744321 1106 14-15 -0.08622398166799172 1106 16-17 0.02772891784116638 1106 18-19 0.021316742498747487 1106 20-21 -0.006979511979842812 1106 22-23 -0.049468641007742065 1106 24-25 -0.005025454981804955 1106 26-27 0.1111220137937039 1106 28-29 0.07554839181539563 1106 30-31 0.09151063047747243 1106 32-33 0.12373300858165237 1106 34-35 -0.06193455336897813 1106 36-37 -0.12485016686247263 1106 38-39 -0.06052174124553744 1106 40-41 -0.02338901661880044 1106 42-43 -0.3479481789385943 1106 44-45 -0.15417105587348345 1106 46-47 -0.0812493948779931 1106 48-49 -0.1505081661122034 1106 50-51 -0.00976798300848003 1106 52-53 -0.07940930240790323 1106 54-55 -0.04290738792342097 1106 56-57 -0.11430571296154568 1106 58-59 -0.0765315056365452 1106 60-61 0.03634146941347183 1106 62-63 0.18902282712573282 1106 64-65 0.05521716296592416 1106 66-67 0.14805310430428875 1106 68-69 0.05385064196464384 1106 70-71 0.14714544182304223 1106 72-73 0.15590285945517124 1106 74-75 -0.0018655065968040674 1106 76-77 0.1381785274639178 1106 78-79 -0.011507123496627969 1106 80-81 0.04504770201263497 1106 82-83 0.12007942358363266 1106 84-85 0.1306559182070437 1106 86-87 0.09122070030878504 1106 88-89 -0.01902624303860634 1106 90-91 0.04936260078109811 1106 92-93 0.163289796776656 1106 94-95 0.38325930116338824 1106 96-97 0.5031455239057756 1106 98-99 0.3772986952326747 1106 100-101 0.49418131551942324 1107 1 0.12292215745618762 1107 2 0.19810434454134196 1107 3 0.13632283471886097 1107 4 -0.001287061771364506 1107 5 0.0995522629284622 1107 6 0.038380975107621396 1107 7 0.09274535499584857 1107 8 0.061149748714058205 1107 9 0.058485682425462926 1107 10-11 0.1314523885607244 1107 12-13 0.19657274638430522 1107 14-15 0.2908980509263088 1107 16-17 0.1911389284198961 1107 18-19 0.1825597794059064 1107 20-21 0.09166393328082023 1107 22-23 0.1985938992172933 1107 24-25 0.20651922347681761 1107 26-27 0.16121707434393784 1107 28-29 0.12374538538679047 1107 30-31 0.15291549225128875 1107 32-33 0.3255798464312889 1107 34-35 0.29793213992943635 1107 36-37 0.18303652983888696 1107 38-39 0.35770491713569186 1107 40-41 0.36248284027993094 1107 42-43 0.4195035695097289 1107 44-45 0.41446469577066125 1107 46-47 0.2645353909953414 1107 48-49 0.5957276950180201 1107 50-51 0.48947597121449604 1107 52-53 0.48812844439814285 1107 54-55 0.5664719813479806 1107 56-57 0.4763733596617499 1107 58-59 0.47738336153759775 1107 60-61 0.5413328880719774 1107 62-63 0.5595638585142098 1107 64-65 0.5627554276115418 1107 66-67 0.6606090494724768 1107 68-69 0.5320466368454291 1107 70-71 0.4362399253520408 1107 72-73 0.5995467485783159 1107 74-75 0.5070463275169885 1107 76-77 0.32137407848221144 1107 78-79 0.44344379270953027 1107 80-81 0.574039505351962 1107 82-83 0.47372667866400775 1107 84-85 0.48602813389462085 1107 86-87 0.6654929507655929 1107 88-89 0.5305724312920894 1107 90-91 0.6956091885881968 1107 92-93 0.6859676561758157 1107 94-95 0.89831863078307 1107 96-97 0.8481946076996145 1107 98-99 1.0047471517008617 1107 100-101 1.12731731498679 1108 1 0.05201601552149526 1108 2 0.12587966925641325 1108 3 -0.030760609730926092 1108 4 -0.06638396260593993 1108 5 -0.06368618788228986 1108 6 -0.08394578158887356 1108 7 -0.07719243436233114 1108 8 -0.04278748979795921 1108 9 0.05092029130283038 1108 10-11 -0.07717862757557015 1108 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0.2513748631919839 2316 80-81 0.1774429165931366 2316 82-83 0.08544417465116894 2316 84-85 0.16765584664044653 2316 86-87 0.27406738081165827 2316 88-89 0.4770554860102756 2316 90-91 0.4878242768707004 2316 92-93 0.3947391395567834 2316 94-95 0.26141419512554975 2316 96-97 0.0885781439587312 2316 98-99 0.19637238496763842 2316 100-101 0.9174052712435561 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 14.0 5 35.0 6 72.0 7 168.0 8 449.0 9 837.0 10 1439.0 11 2456.0 12 3837.0 13 5615.0 14 7296.0 15 10120.0 16 14203.0 17 20002.0 18 18262.0 19 10495.0 20 8552.0 21 8501.0 22 8982.0 23 9526.0 24 11275.0 25 13146.0 26 15814.0 27 19211.0 28 23950.0 29 29369.0 30 36548.0 31 46138.0 32 58830.0 33 75240.0 34 102085.0 35 143937.0 36 216010.0 37 326388.0 38 265480.0 39 24108.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83182411592454 20.383407103810924 15.298965479577454 21.485803300687078 2 15.295371814872594 21.347633905356215 40.769890795631824 22.587103484139366 3 19.18473738949558 25.858814352574104 30.703328133125325 24.25312012480499 4 12.392379860335142 17.55814656424837 37.9026350155389 32.14683855987759 5 12.56815465857328 38.89291997768598 34.4375915934894 14.101333770251346 6 30.433567342693706 37.63494539781591 17.51475559022361 14.416731669266772 7 27.181422256890276 34.53874154966199 20.777041081643265 17.502795111804474 8 26.005720228809153 36.001170046801874 19.677392095683828 18.315717628705148 9 26.574102964118563 16.67336193447738 21.05713728549142 35.695397815912635 10-11 24.42664456578263 27.927587103484143 27.178107124284974 20.467661206448255 12-13 25.357048947907796 25.435575177000914 28.42207458106614 20.78530129402515 14-15 22.33391835673427 26.403796151846077 27.553399635985436 23.708885855434218 16-17 20.51813572542902 29.885140405616223 28.265763130525222 21.33096073842954 18-19 20.44396775871035 27.785848933957357 31.68649245969839 20.083690847633907 20-21 21.610016900676026 26.721691367654703 31.932332293291733 19.735959438377535 22-23 21.13960789921866 26.831212054238872 31.842652660591007 20.186527385951454 24-25 20.66686167446698 26.966621164846593 32.15057852314093 20.215938637545502 26-27 20.66873874837208 27.241850806867056 31.881381068566228 20.208029376194638 28-29 20.362948505644766 27.29712732124372 32.14452286426077 20.195401308850748 30-31 20.64154316172647 27.164814092563706 32.072997919916794 20.120644825793033 32-33 20.47838663546542 27.146548361934475 31.959860894435778 20.415204108164325 34-35 20.77801612064483 27.224551482059283 31.80456968278731 20.192862714508582 36-37 20.927684607384293 27.068902756110248 31.737714508580346 20.265698127925116 38-39 20.6726020598694 27.419683521532296 31.76033387968072 20.14738053891758 40-41 20.76451404683731 27.34019159003347 31.716945453978155 20.17834890915107 42-43 20.632245242004615 27.315754964741085 31.52361500700692 20.52838478624738 44-45 20.817602704108165 27.671346853874155 31.021613364534584 20.4894370774831 46-47 20.938410036401457 27.900773530941237 30.453880655226207 20.7069357774311 48-49 21.049564482579303 27.908411336453458 30.262090483619346 20.779933697347893 50-51 21.255947737909516 28.229329173166928 29.782208788351532 20.73251430057202 52-53 21.250682527301095 28.486446957878314 29.54751690067603 20.715353614144565 54-55 21.160946437857515 28.36807722308892 29.660848933957357 20.810127405096203 56-57 21.236414456578263 28.72227639105564 29.38748049921997 20.653828653146125 58-59 21.224616484659386 28.31740769630785 29.568967758710347 20.88900806032241 60-61 21.306259750390016 28.272783411336455 29.77804862194488 20.642908216328653 62-63 21.195072802912115 28.26140795631825 29.85273660946438 20.69078263130525 64-65 21.222568902756112 28.141413156526262 29.703133125325014 20.932884815392615 66-67 21.041309152366093 28.45290561622465 29.586258450338015 20.919526781071244 68-69 21.13663044283729 28.422553851474063 29.536959876442896 20.90385582924575 70-71 21.08294331773271 28.40694877795112 29.48859204368175 21.021515860634427 72-73 21.152983619344774 28.310289911596463 29.270280811232453 21.266445657826313 74-75 21.11950728029121 28.5446892875715 29.02252340093604 21.31328003120125 76-77 21.058177327093084 28.427002080083202 29.277886115444616 21.236934477379098 78-79 21.05359464378575 28.48345683827353 29.12486999479979 21.338078523140926 80-81 20.849149293892317 28.726399564222145 29.067858555504312 21.356592586381225 82-83 20.99394971584953 28.682182132566712 28.960530955905895 21.363337195677857 84-85 21.02962737378619 28.460733174877785 28.959695890155402 21.549943561180623 86-87 20.9139365574623 28.401066042641705 29.035686427457097 21.649310972438897 88-89 21.11069942797712 28.14589833593344 29.103451638065525 21.639950598023923 90-91 20.95308616613022 28.34798137827062 29.259402042513244 21.43953041308592 92-93 20.833972547173982 28.2770218442121 29.188324098318457 21.70068151029546 94-95 20.5527496099844 28.42953718148726 29.128315132605305 21.889398075923037 96-97 20.760497919916794 28.346496359854395 28.98153926157046 21.911466458658346 98-99 20.778005975760887 28.35720528360488 28.774689503221406 22.090099237412826 100-101 20.785760029647644 28.15784768524087 29.030242545015977 22.02614974009551 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 868.0 1 609.5 2 467.0 3 611.5 4 544.5 5 524.0 6 954.5 7 1984.0 8 3115.0 9 3658.5 10 3356.5 11 2909.0 12 3019.5 13 3231.0 14 3406.5 15 3611.5 16 3774.0 17 3900.0 18 4078.0 19 4308.0 20 4583.0 21 5137.0 22 5913.5 23 7030.5 24 8275.5 25 9793.5 26 12189.0 27 14748.5 28 16742.5 29 19198.0 30 22331.0 31 25395.0 32 28714.5 33 32650.5 34 37257.0 35 41337.5 36 45914.5 37 49583.0 38 51018.0 39 51869.5 40 52709.0 41 54036.0 42 54388.0 43 55764.5 44 58299.0 45 59772.5 46 59962.5 47 59128.0 48 60451.0 49 58929.0 50 54020.5 51 49459.5 52 44378.5 53 41097.5 54 38110.5 55 34087.0 56 29665.0 57 25673.5 58 22371.5 59 19204.5 60 15765.5 61 12583.5 62 10051.5 63 7777.5 64 5780.5 65 4150.0 66 3089.5 67 2596.0 68 2017.5 69 1339.5 70 892.5 71 617.0 72 495.0 73 419.0 74 350.5 75 265.5 76 178.5 77 111.5 78 64.0 79 51.5 80 41.5 81 26.5 82 21.5 83 17.0 84 6.5 85 2.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01924076963078523 2 0.0 3 0.0 4 1.9500780031201246E-4 5 0.023010920436817472 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003932657306292252 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013000520020800832 24-25 0.0 26-27 7.475299011960478E-4 28-29 0.0017875715028601144 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.750390015600623E-5 40-41 7.150286011440457E-4 42-43 0.01810322412896516 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.300052002080083E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.750390015600623E-5 82-83 0.0027626105044201766 84-85 5.525221008840353E-4 86-87 0.0 88-89 0.0 90-91 0.012805512220488818 92-93 0.0033801352054082167 94-95 0.0 96-97 0.0 98-99 0.001202548101924077 100-101 0.021775871034841392 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1538400.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.33855386043942 #Duplication Level Percentage of deduplicated Percentage of total 1 75.43417324296465 42.498522321664815 2 13.834969180649681 15.588843126831032 3 4.7726046096453825 8.066449255652635 4 2.110188308681447 4.755398307372771 5 1.0604565012561353 2.987229285633596 6 0.6513465033472045 2.201755203638122 7 0.43071216966606396 1.6985990538354792 8 0.29958517224384945 1.3502556289799306 9 0.20547026810262514 1.0418307989596807 >10 1.088452267665283 11.046019725931501 >50 0.07803672471505707 2.9948970875756795 >100 0.03205214541774549 3.0706025117757902 >500 0.0012636448290395965 0.5272732642390161 >1k 3.4463040791989E-4 0.35291777462812746 >5k 2.2975360527992665E-4 1.075904105156426 >10k+ 1.1487680263996333E-4 0.7435025481254225 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11488 0.7467498699947998 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9819 0.6382605304212169 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6805 0.44234269370774826 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2354 0.15301612064482578 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2012 0.13078523140925638 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.150286011440457E-4 0.0 0.0 0.0 0.0 7 7.150286011440457E-4 0.0 0.0 0.0 0.0 8 7.150286011440457E-4 0.0 0.0 0.0 0.0 9 7.150286011440457E-4 0.0 0.0 0.0 0.0 10-11 7.475299011960478E-4 0.0 0.0 0.0 0.0 12-13 7.8003120124805E-4 0.0 0.0 0.0 0.0 14-15 8.450338013520541E-4 0.0 0.0 6.500260010400417E-5 0.0 16-17 0.001235049401976079 0.0 0.0 6.500260010400417E-5 0.0 18-19 0.001235049401976079 0.0 0.0 1.3000520020800833E-4 0.0 20-21 0.001235049401976079 0.0 0.0 2.2750910036401458E-4 0.0 22-23 0.001235049401976079 0.0 0.0 4.550182007280291E-4 0.0 24-25 0.001235049401976079 0.0 0.0 8.775351014040562E-4 0.0 26-27 0.001235049401976079 0.0 0.0 0.0012025481019240771 0.0 28-29 0.001235049401976079 0.0 0.0 0.0021775871034841396 0.0 30-31 0.001235049401976079 0.0 0.0 0.004517680707228289 0.0 32-33 0.001235049401976079 0.0 0.0 0.006630265210608425 0.0 34-35 0.001235049401976079 0.0 0.0 0.008840353614144566 0.0 36-37 0.001235049401976079 0.0 0.0 0.01264300572022881 0.0 38-39 0.001235049401976079 0.0 0.0 0.018298231929277174 0.0 40-41 0.001235049401976079 0.0 0.0 0.025221008840353616 0.0 42-43 0.001235049401976079 0.0 0.0 0.03714898595943837 0.0 44-45 0.0013000520020800832 0.0 0.0 0.046476859074362976 0.0 46-47 0.0013650546021840874 0.0 0.0 0.06766770670826833 0.0 48-49 0.0013650546021840874 0.0 0.0 0.09279121164846593 0.0 50-51 0.0013650546021840874 0.0 0.0 0.10887935517420697 0.0 52-53 0.0013650546021840874 0.0 0.0 0.13501040041601664 0.0 54-55 0.0013650546021840874 0.0 0.0 0.17771710868434737 0.0 56-57 0.0013650546021840874 0.0 0.0 0.2419721788871555 0.0 58-59 0.0013650546021840874 0.0 0.0 0.3218278731149246 0.0 60-61 0.0013975559022360894 0.0 0.0 0.41731669266770666 0.0 62-63 0.0018200728029121164 0.0 0.0 0.5133255330213209 0.0 64-65 0.0018200728029121164 0.0 0.0 0.6191172646905876 0.0 66-67 0.0018525741029641186 0.0 0.0 0.7458723348933958 0.0 68-69 0.0018850754030161206 0.0 0.0 0.906233749349974 0.0 70-71 0.002080083203328133 0.0 0.0 1.0271710868434738 0.0 72-73 0.00234009360374415 0.0 0.0 1.1662766510660427 0.0 74-75 0.00234009360374415 0.0 0.0 1.3180902236089445 0.0 76-77 0.00234009360374415 0.0 0.0 1.483749349973999 0.0 78-79 0.00234009360374415 0.0 0.0 1.6812272490899636 0.0 80-81 0.00234009360374415 0.0 0.0 1.941205148205928 0.0 82-83 0.00234009360374415 0.0 0.0 2.2083983359334374 0.0 84-85 0.00234009360374415 0.0 0.0 2.552522100884035 0.0 86-87 0.00234009360374415 0.0 0.0 2.955245709828393 0.0 88-89 0.002405096203848154 0.0 0.0 3.47435647425897 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6085 0.0 42.24694 1 GTATCAA 9515 0.0 33.959297 1 GTACATG 8470 0.0 27.377577 1 TACATGG 8470 0.0 26.979599 2 ATCAACG 11930 0.0 26.800844 3 TATCAAC 12090 0.0 26.681934 2 TCAACGC 12055 0.0 26.522943 4 CAACGCA 12235 0.0 26.13274 5 AACGCAG 12570 0.0 25.392708 6 ACATGGG 8750 0.0 25.03034 3 ACGCAGA 14270 0.0 22.301804 7 CGCAGAG 14265 0.0 22.209726 8 GAGTACT 9065 0.0 22.085735 12-13 CATGGGA 5485 0.0 21.913801 4 AGTACTT 9250 0.0 21.669697 12-13 CTATACG 110 0.0028747197 21.5949 4 GTACTTT 9590 0.0 20.826118 14-15 GTATAGG 480 0.0 20.789145 1 GCAGAGT 15125 0.0 20.695656 9 CATGGGG 2975 0.0 19.48259 4 >>END_MODULE