FastQCFastQC Report
Thu 26 May 2016
SRR937865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386651
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86970.6271945860926794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67870.4894526452582517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46160.3328883763830986No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21120.15230941311115775No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.1142320598333683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51300.040.403121
GTATCAA81500.036.339271
ATCAACG102350.028.7857023
TCAACGC103600.028.3945714
TATCAAC104450.028.2089942
CAACGCA104950.027.9840415
AACGCAG108100.027.1568076
GTACATG82900.024.7727951
TACATGG82900.024.363452
CGCAGAG122500.023.7706098
ACGCAGA122700.023.7318637
GTATAGG3650.023.443661
GAGTACT65800.023.38772412-13
ACATGGG85800.022.5967453
GCAGAGT127650.022.55119
AGTACTT69000.021.64912812-13
GTACTTT71200.021.5813614-15
CATGGGG27200.020.9660554
GTGTAGC8900.020.2973521
GCGTGCG3153.2196112E-1019.6041819
Produced by FastQC (version 0.11.5)