FastQCFastQC Report
Thu 26 May 2016
SRR937865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386651
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107440.7748164462435032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90440.6522189072809237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63490.45786574992553997No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.1512997863197012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.1344245956624991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52750.043.1536521
GTATCAA85400.037.228261
CTATCGT600.00395230431.6649116
TATCAAC110650.028.675452
ATCAACG110450.028.6413653
TCAACGC111350.028.3672084
CAACGCA113050.027.8986195
AACGCAG116200.027.1413546
TAGGTCG908.9494925E-426.388385
GTACATG79150.025.4577181
GGACCGA1501.0608874E-625.3319286
TACATGG79400.024.5870532
ACATGGG80450.023.4395713
ACGCAGA134700.023.3809667
CGCAGAG134600.023.221888
GAGTACT85500.022.4456312-13
GCAGAGT141750.021.7824549
AGTACTT87300.021.24825912-13
GTACTTT90500.020.9670714-15
CATGGGG26950.020.621124
Produced by FastQC (version 0.11.5)