##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937865_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1386651 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18899131793076 33.0 31.0 34.0 31.0 34.0 2 32.396041253350695 34.0 31.0 34.0 31.0 34.0 3 32.45026470250986 34.0 31.0 34.0 31.0 34.0 4 36.07268231155496 37.0 35.0 37.0 35.0 37.0 5 35.99039484340328 37.0 35.0 37.0 35.0 37.0 6 36.03496553927412 37.0 35.0 37.0 35.0 37.0 7 35.98271158352029 37.0 35.0 37.0 35.0 37.0 8 35.960896433204894 37.0 35.0 37.0 35.0 37.0 9 37.63816346001986 39.0 37.0 39.0 35.0 39.0 10-11 37.55708105355998 39.0 37.0 39.0 35.0 39.0 12-13 37.45106627406608 39.0 37.0 39.0 34.5 39.0 14-15 38.64107983912318 40.0 38.0 41.0 34.0 41.0 16-17 38.607151330796285 40.0 38.0 41.0 34.0 41.0 18-19 38.619389449832724 40.0 38.0 41.0 34.0 41.0 20-21 38.53841665999592 40.0 38.0 41.0 34.0 41.0 22-23 38.480000735585236 40.0 38.0 41.0 34.0 41.0 24-25 38.487100575415155 40.0 38.0 41.0 34.0 41.0 26-27 38.29133177706575 40.0 38.0 41.0 34.0 41.0 28-29 38.25723163218431 40.0 38.0 41.0 34.0 41.0 30-31 38.235184988868866 40.0 37.5 41.0 34.0 41.0 32-33 38.07555578151965 40.0 37.0 41.0 34.0 41.0 34-35 37.91492199551293 40.0 37.0 41.0 33.0 41.0 36-37 37.72792973862926 40.0 37.0 41.0 33.0 41.0 38-39 37.57407812059415 40.0 37.0 41.0 33.0 41.0 40-41 37.37353739333113 40.0 36.0 41.0 33.0 41.0 42-43 37.12108418051838 40.0 36.0 41.0 32.0 41.0 44-45 36.755029203454946 40.0 36.0 41.0 31.0 41.0 46-47 36.32407866146565 39.5 36.0 41.0 30.0 41.0 48-49 35.911367748626006 39.0 35.0 41.0 29.0 41.0 50-51 35.64774481827078 39.0 35.0 41.0 28.0 41.0 52-53 35.38144132878424 39.0 35.0 40.0 27.0 41.0 54-55 35.15408563510213 39.0 35.0 40.0 26.5 41.0 56-57 34.899831680790626 38.0 35.0 40.0 26.0 41.0 58-59 34.6537221694572 38.0 34.0 40.0 25.5 41.0 60-61 34.328265367421224 38.0 34.0 40.0 24.5 41.0 62-63 33.9334313392483 37.5 34.0 40.0 23.0 41.0 64-65 33.57363604829189 37.0 33.0 40.0 22.0 41.0 66-67 33.16104052137128 36.5 33.0 39.0 21.5 40.5 68-69 32.68501807592538 36.0 32.0 39.0 19.5 40.0 70-71 32.2759273962951 35.5 32.0 38.5 18.0 40.0 72-73 31.803217608468174 35.0 31.0 38.0 17.0 40.0 74-75 31.317480750383478 35.0 31.0 37.0 12.5 39.0 76-77 30.02636135552493 33.5 29.5 36.0 11.0 38.5 78-79 30.628071879658258 34.0 30.5 36.0 8.0 39.0 80-81 30.45780913870902 34.0 31.0 36.0 7.0 37.5 82-83 30.058079141759535 34.0 30.0 35.5 4.5 37.0 84-85 29.636929912429302 34.0 30.0 35.0 2.0 37.0 86-87 29.27713065508192 34.0 29.0 35.0 2.0 36.0 88-89 28.875449193776948 34.0 29.0 35.0 2.0 36.0 90-91 28.376339107677417 34.0 29.0 35.0 2.0 35.5 92-93 27.91374614088188 33.5 27.5 35.0 2.0 35.0 94-95 27.40638487982917 33.0 27.0 35.0 2.0 35.0 96-97 26.894691238098126 33.0 26.0 35.0 2.0 35.0 98-99 26.083649021996163 32.5 24.5 34.5 2.0 35.0 100-101 23.655049828687968 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1799001617198499 1101 2 0.19695290479377547 1101 3 0.21886264806303046 1101 4 0.16821010739964493 1101 5 0.1602428521391417 1101 6 0.12469425188299255 1101 7 0.057785042973335976 1101 8 0.061691939776075344 1101 9 0.10286601496999026 1101 10-11 0.10972513453440769 1101 12-13 0.1672767367004937 1101 14-15 0.2739787902530608 1101 16-17 0.3060404939919792 1101 18-19 0.2635827849016579 1101 20-21 0.2595687341173232 1101 22-23 0.19078036603162474 1101 24-25 0.19757183678096624 1101 26-27 0.16299150780849203 1101 28-29 0.2913591046979178 1101 30-31 0.4094334010388394 1101 32-33 0.38304608139246454 1101 34-35 0.3329994691928988 1101 36-37 0.3652798724019988 1101 38-39 0.48136496120239514 1101 40-41 0.3983535768175912 1101 42-43 0.5238672277988883 1101 44-45 0.7035055136167969 1101 46-47 0.8159089910120372 1101 48-49 0.7808028812165375 1101 50-51 0.8878292830353871 1101 52-53 0.8417023416552709 1101 54-55 0.9351088400872243 1101 56-57 1.0053986106975685 1101 58-59 0.9777219386668534 1101 60-61 0.9716333128007193 1101 62-63 0.7425201617339425 1101 64-65 0.9314397957096432 1101 66-67 0.9526958052577541 1101 68-69 1.0508076326475404 1101 70-71 0.9742815254655177 1101 72-73 0.9480148554169538 1101 74-75 0.9350982890093213 1101 76-77 0.9951103309011167 1101 78-79 1.0122928168236065 1101 80-81 1.0758942432652887 1101 82-83 1.0625779688574326 1101 84-85 1.2056299884753408 1101 86-87 1.0888767100350094 1101 88-89 1.1448788862471915 1101 90-91 1.0687220089731326 1101 92-93 1.1981235316831444 1101 94-95 1.1401616766541096 1101 96-97 0.9983323324340141 1101 98-99 1.2329244017794174 1101 100-101 1.6460075316478644 1102 1 0.15474684531447735 1102 2 0.20830266510489537 1102 3 0.18646313307802842 1102 4 0.11536168314076178 1102 5 0.10911798186018018 1102 6 0.21978303966174195 1102 7 0.13829588225185319 1102 8 0.16167197270416978 1102 9 0.09344360415458652 1102 10-11 0.1606976164872762 1102 12-13 0.21487505848871535 1102 14-15 0.20383402363483327 1102 16-17 0.19025538551669996 1102 18-19 0.23575560716553667 1102 20-21 0.2306147993896417 1102 22-23 0.28279640935237893 1102 24-25 0.34498160952451684 1102 26-27 0.34546472680143836 1102 28-29 0.3612702856012717 1102 30-31 0.36357979191039647 1102 32-33 0.3342829628199837 1102 34-35 0.3772712187499039 1102 36-37 0.4424571120177063 1102 38-39 0.4016678526696751 1102 40-41 0.4334326056326603 1102 42-43 0.45598353729125307 1102 44-45 0.5806551629250478 1102 46-47 0.528812745600689 1102 48-49 0.6084511497108664 1102 50-51 0.5677295454488416 1102 52-53 0.7244830260568236 1102 54-55 0.6751446677666593 1102 56-57 0.5736334436287933 1102 58-59 0.6846642161077128 1102 60-61 0.5527805165627839 1102 62-63 0.5953453496375332 1102 64-65 0.6749736802278576 1102 66-67 0.49874816848370074 1102 68-69 0.4171810211696396 1102 70-71 0.5042835405051385 1102 72-73 0.621427881861333 1102 74-75 0.61604159031641 1102 76-77 0.6492345853631925 1102 78-79 0.5849482097586218 1102 80-81 0.6695747184785468 1102 82-83 0.6690155239406543 1102 84-85 0.6939833191800062 1102 86-87 0.7153752907229851 1102 88-89 0.7253769692096874 1102 90-91 0.6946673321944985 1102 92-93 0.8232604711175284 1102 94-95 1.0321686148678602 1102 96-97 1.1901356126251947 1102 98-99 1.2817352610555588 1102 100-101 1.7023402634228688 1103 1 0.1213247913268134 1103 2 0.11939970336340622 1103 3 0.11182606874817935 1103 4 0.06276736647142656 1103 5 -0.02051697839543465 1103 6 0.09492987936966557 1103 7 0.08165379281704332 1103 8 0.04434019241064391 1103 9 0.1214358444384942 1103 10-11 0.11614161727133165 1103 12-13 0.21106682120945663 1103 14-15 0.11283692547531388 1103 16-17 0.09323057731643303 1103 18-19 0.09403995394986708 1103 20-21 0.14687598627941156 1103 22-23 0.13273753639597174 1103 24-25 -0.00927072791689909 1103 26-27 0.1703115988586461 1103 28-29 -0.025743498483706162 1103 30-31 0.05747742835652048 1103 32-33 0.1474030478876358 1103 34-35 0.24091384414107608 1103 36-37 0.28559272405657055 1103 38-39 0.39467746013303895 1103 40-41 0.30324224174433567 1103 42-43 0.4368715868350037 1103 44-45 0.5731650527592862 1103 46-47 0.5934114225697726 1103 48-49 0.7142209735854621 1103 50-51 0.6439620566168855 1103 52-53 0.5738536714113494 1103 54-55 0.6794084165552761 1103 56-57 0.8052487523794127 1103 58-59 0.6487486285501234 1103 60-61 0.7916707304929886 1103 62-63 0.5642876376914572 1103 64-65 0.6482822649877775 1103 66-67 0.6593141336095201 1103 68-69 0.6498215520817112 1103 70-71 0.7365046273040257 1103 72-73 0.7330162077447362 1103 74-75 0.6784457247194631 1103 76-77 0.6792615922182215 1103 78-79 0.7134791307400121 1103 80-81 0.7736085954443475 1103 82-83 0.7216073561575271 1103 84-85 0.7108730554099729 1103 86-87 0.8427626782029911 1103 88-89 0.8599089566208811 1103 90-91 0.9234413697004804 1103 92-93 1.1285349979749775 1103 94-95 1.1058613302476168 1103 96-97 1.0954084089329896 1103 98-99 1.204596096078319 1103 100-101 1.671192168809437 1104 1 0.09393251006855508 1104 2 0.13088011038080793 1104 3 0.07241926214837235 1104 4 0.06655345814737501 1104 5 0.09761309719255706 1104 6 0.14287588699203724 1104 7 0.07100154107122592 1104 8 0.06091960999162893 1104 9 -0.0011825817653416948 1104 10-11 0.16148353665084159 1104 12-13 0.09023311144191126 1104 14-15 0.13137672879262396 1104 16-17 0.1595078855273826 1104 18-19 0.1414742201708421 1104 20-21 0.10893748224570743 1104 22-23 0.14847834219941802 1104 24-25 0.08493214335598509 1104 26-27 0.08261560211104069 1104 28-29 0.17413428587470037 1104 30-31 0.24121831446593944 1104 32-33 0.2057313202720934 1104 34-35 0.20408603016984728 1104 36-37 0.2401357831115689 1104 38-39 0.19348173776414512 1104 40-41 0.44971593150231115 1104 42-43 0.43246856130774347 1104 44-45 0.5162952538253407 1104 46-47 0.4447153070362617 1104 48-49 0.5250552521869309 1104 50-51 0.54036255083318 1104 52-53 0.5556917662192333 1104 54-55 0.6097904638220228 1104 56-57 0.4515873440804512 1104 58-59 0.5632211020073328 1104 60-61 0.6405784020549703 1104 62-63 0.62043070946158 1104 64-65 0.8832269877146999 1104 66-67 0.8406816019974102 1104 68-69 0.6565952766389387 1104 70-71 0.8548208286980064 1104 72-73 0.8586806141330747 1104 74-75 0.8387176106046859 1104 76-77 0.7115452178318087 1104 78-79 0.7180354486445637 1104 80-81 0.7608748716232547 1104 82-83 0.586947845087014 1104 84-85 0.6306735369111607 1104 86-87 0.8152735696995919 1104 88-89 0.5326117459147603 1104 90-91 0.6609993823955165 1104 92-93 0.7722172189033785 1104 94-95 0.7129282134057817 1104 96-97 0.8092238792080728 1104 98-99 0.894300118403379 1104 100-101 0.9863962212492332 1105 1 0.07144730296796098 1105 2 0.0983582019095337 1105 3 0.05254060137389871 1105 4 0.04659989661640651 1105 5 0.03347557940362833 1105 6 0.10025735654487988 1105 7 0.06168388696291771 1105 8 0.07482119039758572 1105 9 0.041391008227918746 1105 10-11 0.04452975041759544 1105 12-13 0.0583441143630381 1105 14-15 0.01960607490901367 1105 16-17 0.09291362292533023 1105 18-19 0.21581322099474676 1105 20-21 0.2368155438141244 1105 22-23 0.11933445784618613 1105 24-25 0.1033914347948297 1105 26-27 0.05738153724698236 1105 28-29 0.12014956562902057 1105 30-31 0.10966637079130237 1105 32-33 0.053858801359652375 1105 34-35 0.34138660690566525 1105 36-37 0.16536610293780285 1105 38-39 0.14742151755591948 1105 40-41 0.10134127791830849 1105 42-43 0.31905758085684255 1105 44-45 0.38842842737039973 1105 46-47 0.2868806879066099 1105 48-49 0.19789998291871314 1105 50-51 0.21554418516768692 1105 52-53 0.2353374856410042 1105 54-55 0.3138013993324762 1105 56-57 0.251965592900774 1105 58-59 0.4028286220432875 1105 60-61 0.35434890613706216 1105 62-63 0.2600307394218717 1105 64-65 0.29185929990105564 1105 66-67 0.3643876914289166 1105 68-69 0.43766036539634 1105 70-71 0.26341194546828106 1105 72-73 0.2785592749075505 1105 74-75 0.36851313153944787 1105 76-77 0.2634065016307794 1105 78-79 0.30318637366890755 1105 80-81 0.4677282908427891 1105 82-83 0.3070998936227092 1105 84-85 0.39976948478022933 1105 86-87 0.6635695774892731 1105 88-89 0.49161481684577524 1105 90-91 0.4569734667337677 1105 92-93 0.49217827051000285 1105 94-95 0.6100595144319207 1105 96-97 0.556497491671557 1105 98-99 0.4369223938883593 1105 100-101 0.7260074863230095 1106 1 -0.28158444609356437 1106 2 -0.2252149990830219 1106 3 -0.21931153325739672 1106 4 -0.2056874355716687 1106 5 -0.22728665257448455 1106 6 -0.11683241885754825 1106 7 -0.255819453369206 1106 8 -0.17377587011014128 1106 9 -0.2724602356481398 1106 10-11 -0.3591318898445408 1106 12-13 -0.6476112206245475 1106 14-15 -0.4276007711812113 1106 16-17 -0.3119378118268443 1106 18-19 -0.5070505570799568 1106 20-21 -0.4550054143840043 1106 22-23 -0.48685947229315474 1106 24-25 -0.32643932171086476 1106 26-27 -0.5157526486663571 1106 28-29 -0.5038085688750158 1106 30-31 -0.49355307312292496 1106 32-33 -0.6155405751116874 1106 34-35 -0.802960278632284 1106 36-37 -0.5345289151954304 1106 38-39 -0.3770965202375507 1106 40-41 -0.5352910310465049 1106 42-43 -0.66651218436553 1106 44-45 -0.6875529940204501 1106 46-47 -0.7481132676828821 1106 48-49 -0.794468153494087 1106 50-51 -0.6848471108811793 1106 52-53 -0.6689158705974663 1106 54-55 -0.7064950949094282 1106 56-57 -0.7107944758146374 1106 58-59 -0.8021373383817405 1106 60-61 -0.7300883274316874 1106 62-63 -0.6715147209077017 1106 64-65 -1.1598204099672387 1106 66-67 -0.9060360535605838 1106 68-69 -0.9805014975546058 1106 70-71 -0.8219860312448297 1106 72-73 -0.828083214117072 1106 74-75 -0.7797085392628667 1106 76-77 -0.906587772085583 1106 78-79 -0.8253496064799783 1106 80-81 -1.001152538832084 1106 82-83 -1.0971121061227933 1106 84-85 -0.9362297517152811 1106 86-87 -0.9145114812369499 1106 88-89 -1.1974193499799561 1106 90-91 -1.2540941670385806 1106 92-93 -1.3087315723353257 1106 94-95 -1.2191031756488826 1106 96-97 -1.3946191336386136 1106 98-99 -1.3660520923367478 1106 100-101 -0.4039855148858713 1107 1 -0.04968753310836149 1107 2 0.014583630838060913 1107 3 -0.030970887705137784 1107 4 -0.006849449174715971 1107 5 -0.026623360402162177 1107 6 -0.06605678607876797 1107 7 -0.039649003530293214 1107 8 -0.06416039386294159 1107 9 0.0014724675026513978 1107 10-11 -0.04166368913909224 1107 12-13 0.0207347517906058 1107 14-15 -0.02927604512979798 1107 16-17 -0.1035933299558991 1107 18-19 0.14302647473339647 1107 20-21 -0.009154319750329876 1107 22-23 0.02818922085388209 1107 24-25 -0.10303484543556607 1107 26-27 -0.14336094830972712 1107 28-29 -0.0904296517675931 1107 30-31 0.018256740766744883 1107 32-33 0.12570553206332846 1107 34-35 0.11025858823646217 1107 36-37 0.1450602810469377 1107 38-39 0.34184304308186597 1107 40-41 0.3200528753766392 1107 42-43 0.27495157891650024 1107 44-45 0.49104963300262483 1107 46-47 0.5119796714137266 1107 48-49 0.3795996149535341 1107 50-51 0.5051633717985808 1107 52-53 0.5037676465492851 1107 54-55 0.5199689677032069 1107 56-57 0.6537791979980909 1107 58-59 0.752711939498468 1107 60-61 0.8090140228633018 1107 62-63 0.5946723448338531 1107 64-65 0.5076000811059842 1107 66-67 0.7123201816179758 1107 68-69 0.7972611100669624 1107 70-71 0.6706357749282184 1107 72-73 0.7492824444774406 1107 74-75 0.8328012806134168 1107 76-77 0.6731199957267258 1107 78-79 0.9265416742029373 1107 80-81 0.9794328174263072 1107 82-83 0.9000613577919871 1107 84-85 1.0051084759787834 1107 86-87 1.2821242871813503 1107 88-89 1.1965635551884475 1107 90-91 1.1060002381670841 1107 92-93 1.2416442402861634 1107 94-95 1.4152516691695816 1107 96-97 1.474052314987091 1107 98-99 1.7850393036800796 1107 100-101 2.3770379636289114 1108 1 -0.009622550008245412 1108 2 -0.04166409463450549 1108 3 0.002541077795775948 1108 4 0.023389575412771535 1108 5 -0.06864354512352122 1108 6 0.03215284864104717 1108 7 0.05609641114133268 1108 8 0.046446456002790626 1108 9 -0.02790550722244234 1108 10-11 -0.030570536388374592 1108 12-13 0.14269937263134125 1108 14-15 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0.515882001728027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 11.0 5 27.0 6 78.0 7 189.0 8 461.0 9 841.0 10 1359.0 11 2372.0 12 3530.0 13 5367.0 14 6798.0 15 9751.0 16 13014.0 17 18759.0 18 17031.0 19 9958.0 20 7909.0 21 7767.0 22 7830.0 23 8463.0 24 9730.0 25 11342.0 26 13581.0 27 16426.0 28 20482.0 29 25368.0 30 31754.0 31 39692.0 32 49978.0 33 64930.0 34 88184.0 35 125787.0 36 192551.0 37 302297.0 38 250748.0 39 22281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94736705406866 20.388493807315786 15.256505619120523 21.407633519495032 2 15.330317433874855 21.338462237433934 40.80385042811782 22.527369900573397 3 19.197837090947903 25.915965877499097 30.690563090496454 24.195633941056546 4 12.40059683366615 17.57940534424307 37.9558374818177 32.06416034027308 5 12.641774790303852 38.94052058015333 34.30960743727146 14.108097192271355 6 30.416305184217222 37.56338112473867 17.53620774080861 14.484105950235495 7 27.172951232862484 34.69077655444665 20.679248058812203 17.45702415387866 8 26.056448233910334 35.86028495995027 19.68815513059883 18.395111675540566 9 26.60597367326025 16.691582813555826 21.003770956066088 35.69867255711783 10-11 24.372354687661137 27.98977536525052 27.12697715575152 20.510892791336826 12-13 25.40277592467717 25.487986499010145 28.381395751371198 20.727841824941493 14-15 22.35731990241236 26.434914048307757 27.56014310738607 23.647622941893815 16-17 20.502094615011277 29.894580539732058 28.276978129320213 21.326346715936452 18-19 20.40733392901314 27.76203240757768 31.76642861109248 20.064205052316698 20-21 21.58224119043996 26.74180955931827 32.02509362512593 19.650855625115838 22-23 21.133847387611926 26.84278506646358 31.931835883411797 20.091531662512693 24-25 20.618129579829386 26.890183615055268 32.28898259187063 20.20270421324472 26-27 20.64221076680763 27.18698977089812 32.063626109883515 20.107173352410733 28-29 20.341548724420804 27.304173803299374 32.24328856035338 20.110988911926437 30-31 20.614920408956543 27.123767984878675 32.19750319294473 20.063808413220052 32-33 20.460519626063085 27.108154827710795 32.06855942843585 20.362766117790272 34-35 20.712926323927217 27.247447266832104 31.913401425448797 20.126224983791886 36-37 20.892216337527618 27.063897975549672 31.83403430430794 20.209851382614765 38-39 20.666122910523267 27.334707868093698 31.891946856130343 20.10722236525268 40-41 20.700538529078226 27.32965659982079 31.85117342367112 20.118631447429863 42-43 20.645517762720594 27.32776737672346 31.583960552373526 20.44275430818242 44-45 20.822182366002693 27.66283657531708 31.127370910200185 20.387610148480043 46-47 20.925200356830956 27.855026246690766 30.531330522243884 20.688442874234397 48-49 21.051656112460886 27.948885480196534 30.327746491366607 20.671711915975973 50-51 21.203280421677842 28.294141784774972 29.783233127874283 20.719344665672907 52-53 21.232775947228248 28.48910792982517 29.65021479809988 20.627901324846697 54-55 21.139205178520047 28.392436164543206 29.70444618004098 20.763912476895776 56-57 21.259747405800017 28.720312465068716 29.432207527344662 20.587732601786605 58-59 21.231045158442896 28.34166636017282 29.6127864906166 20.814501990767685 60-61 21.291478533531507 28.31610116748915 29.780673002795943 20.611747296183395 62-63 21.177715228994174 28.247590778068886 29.93236942821229 20.64232456472465 64-65 21.236706280094992 28.07559364252433 29.76491561322928 20.9227844641514 66-67 21.011271040802622 28.445369454895285 29.64841189311514 20.894947611186954 68-69 21.157522693165042 28.445766093991924 29.56515374091967 20.83155747192336 70-71 21.10195716153524 28.4747928642463 29.452760644170738 20.97048933004772 72-73 21.18532348802979 28.28952634801403 29.283936621399327 21.24121354255685 74-75 21.147859122446818 28.470970705678646 29.117780898005336 21.263389273869198 76-77 21.01448021167547 28.44749688277728 29.333516508479786 21.204506397067465 78-79 20.9748523601108 28.46040568246805 29.155858251283128 21.408883706138027 80-81 20.847999965384222 28.672391250574226 29.11897081529527 21.360637968746282 82-83 21.014242959650957 28.704143078642772 28.952078750946526 21.329535210759744 84-85 21.02044495727112 28.46807052969387 28.873832618180508 21.637651894854507 86-87 20.91957529327855 28.32962295487473 29.06719138413343 21.68361036771329 88-89 21.099577326955377 28.1461232855275 29.128526211714412 21.625773175802706 90-91 20.84664312075418 28.387946435333006 29.313254636998913 21.4521558069139 92-93 20.745856024877735 28.2675553494591 29.25423412228932 21.73235450337385 94-95 20.560508736516976 28.40941952949949 29.177132530103105 21.852939203880428 96-97 20.804153316155254 28.293204274182905 28.949425630529962 21.95321677913188 98-99 20.782546581140952 28.33263440137108 28.80074453381719 22.084074483670772 100-101 20.680634502993573 28.237301753877293 28.969268019309457 22.112795723819684 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 775.0 1 562.0 2 419.5 3 535.5 4 482.5 5 458.0 6 842.0 7 1785.5 8 2840.5 9 3350.0 10 3118.5 11 2757.5 12 2815.5 13 2973.0 14 3119.0 15 3305.0 16 3496.5 17 3600.5 18 3777.5 19 4036.5 20 4274.5 21 4736.0 22 5395.0 23 6367.5 24 7626.0 25 9030.0 26 11037.0 27 13476.0 28 15324.5 29 17334.5 30 20088.0 31 22771.5 32 25834.0 33 29524.0 34 33497.0 35 37024.0 36 41029.0 37 44462.5 38 45962.5 39 46765.0 40 47427.5 41 48562.5 42 49154.0 43 50240.0 44 52425.0 45 53939.5 46 54028.0 47 53274.5 48 54842.0 49 53160.5 50 48317.0 51 44607.0 52 40052.0 53 37007.5 54 34461.5 55 30653.0 56 26580.5 57 23055.5 58 20017.5 59 17160.0 60 14139.5 61 11332.0 62 8977.0 63 6920.0 64 5177.0 65 3788.5 66 2806.5 67 2305.5 68 1758.5 69 1127.0 70 766.5 71 547.0 72 456.5 73 383.5 74 313.0 75 236.5 76 140.0 77 81.5 78 53.0 79 42.0 80 37.0 81 32.0 82 21.0 83 11.0 84 6.0 85 2.5 86 2.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04182739564605658 2 0.0 3 0.0 4 0.0 5 0.00872606012616008 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006201993147518734 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.8846479755901085E-4 22-23 0.0019471373835233234 24-25 0.0 26-27 0.0013702077884053016 28-29 0.0018750211841335707 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.1634859816925815E-4 38-39 0.0 40-41 2.5240669786413453E-4 42-43 0.022103615112959208 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.211619938975271E-5 84-85 7.211619938975271E-5 86-87 0.0 88-89 0.0 90-91 0.01009626791456538 92-93 0.0021274278819977052 94-95 0.0 96-97 0.0 98-99 8.293362929821563E-4 100-101 0.01298091589015549 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1386651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.494564901598885 #Duplication Level Percentage of deduplicated Percentage of total 1 76.24501900457739 43.836741935823156 2 13.626797359903406 15.669335704198057 3 4.5092730708173825 7.777760796874264 4 1.9445612730087596 4.472068172645516 5 1.0286870176447107 2.9571956249703013 6 0.626044838793845 2.1596505369206236 7 0.3935153209487526 1.5837494512043102 8 0.2758120515693389 1.2686155119677198 9 0.20731941838326276 1.0727765780038412 >10 1.0384531690471226 10.799057554244616 >50 0.07376757123754021 2.8712113383940827 >100 0.02887493988408182 2.8184326356254203 >500 0.001249976122976117 0.5502843427917251 >1k 2.499952245952234E-4 0.28473639364513714 >5k 2.499952245952234E-4 1.1062461654163542 >10k+ 1.249976122976117E-4 0.7721372572749503 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10744 0.7748164462435032 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9044 0.6522189072809237 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6349 0.45786574992553997 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2098 0.1512997863197012 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1864 0.1344245956624991 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.9327819328728E-4 0.0 0.0 0.0 0.0 7 7.9327819328728E-4 0.0 0.0 0.0 0.0 8 7.9327819328728E-4 0.0 0.0 0.0 0.0 9 7.9327819328728E-4 0.0 0.0 0.0 0.0 10-11 7.9327819328728E-4 0.0 0.0 7.211619938975273E-5 0.0 12-13 7.9327819328728E-4 0.0 0.0 7.211619938975273E-5 0.0 14-15 9.375105920667854E-4 0.0 0.0 7.211619938975273E-5 0.0 16-17 0.0016586725859643126 0.0 0.0 1.4423239877950545E-4 0.0 18-19 0.0016586725859643126 0.0 0.0 2.5240669786413453E-4 0.0 20-21 0.0016586725859643126 0.0 0.0 3.2452289725388723E-4 0.0 22-23 0.001694730685659189 0.0 0.0 4.326971963385163E-4 0.0 24-25 0.0017307887853540652 0.0 0.0 5.769295951180218E-4 0.0 26-27 0.0017307887853540652 0.0 0.0 9.375105920667854E-4 0.0 28-29 0.0017307887853540652 0.0 0.0 0.0016586725859643126 0.0 30-31 0.0017307887853540652 0.0 0.0 0.004399088162774916 0.0 32-33 0.0017307887853540652 0.0 0.0 0.006778922742636756 0.0 34-35 0.0017668468850489416 0.0 0.0 0.008834234425244707 0.0 36-37 0.001802904984743818 0.0 0.0 0.013449671186188882 0.0 38-39 0.0018389630844386944 0.0 0.0 0.01932714143645373 0.0 40-41 0.0018750211841335707 0.0 0.0 0.026791168073293136 0.0 42-43 0.0018750211841335707 0.0 0.0 0.03897880577016134 0.0 44-45 0.0018750211841335707 0.0 0.0 0.04925536418320111 0.0 46-47 0.0018750211841335707 0.0 0.0 0.07240466418731173 0.0 48-49 0.0018750211841335707 0.0 0.0 0.09998911045389214 0.0 50-51 0.0020192535829130762 0.0 0.0 0.11455658273062219 0.0 52-53 0.0020192535829130762 0.0 0.0 0.14015783351398442 0.0 54-55 0.0020192535829130762 0.0 0.0 0.18375207604508995 0.0 56-57 0.0020192535829130762 0.0 0.0 0.250531676680001 0.0 58-59 0.0020192535829130762 0.0 0.0 0.34143414601078426 0.0 60-61 0.0020553116826079526 0.0 0.0 0.44495695023477433 0.0 62-63 0.0024519507792515926 0.0 0.0 0.548587928757849 0.0 64-65 0.0024519507792515926 0.0 0.0 0.6615218970022017 0.0 66-67 0.0026322412777259746 0.0 0.0 0.7997686512323576 0.0 68-69 0.0027404155768106036 0.0 0.0 0.975804293942744 0.0 70-71 0.0028125317762003562 0.0 0.0 1.0984739491047133 0.0 72-73 0.00317311277314912 0.0 0.0 1.2471414941466887 0.0 74-75 0.00317311277314912 0.0 0.0 1.41005198856814 0.0 76-77 0.003209170872843996 0.0 0.0 1.5896573831483192 0.0 78-79 0.0032452289725388725 0.0 0.0 1.799984278668533 0.0 80-81 0.0032452289725388725 0.0 0.0 2.0774513558206067 0.0 82-83 0.0032452289725388725 0.0 0.0 2.35942569543454 0.0 84-85 0.0032452289725388725 0.0 0.0 2.7330236663731537 0.0 86-87 0.0032452289725388725 0.0 0.0 3.175961363025015 0.0 88-89 0.003317345171928625 0.0 0.0 3.7273257654593692 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5275 0.0 43.153652 1 GTATCAA 8540 0.0 37.22826 1 CTATCGT 60 0.003952304 31.664911 6 TATCAAC 11065 0.0 28.67545 2 ATCAACG 11045 0.0 28.641365 3 TCAACGC 11135 0.0 28.367208 4 CAACGCA 11305 0.0 27.898619 5 AACGCAG 11620 0.0 27.141354 6 TAGGTCG 90 8.9494925E-4 26.38838 5 GTACATG 7915 0.0 25.457718 1 GGACCGA 150 1.0608874E-6 25.331928 6 TACATGG 7940 0.0 24.587053 2 ACATGGG 8045 0.0 23.439571 3 ACGCAGA 13470 0.0 23.380966 7 CGCAGAG 13460 0.0 23.22188 8 GAGTACT 8550 0.0 22.44563 12-13 GCAGAGT 14175 0.0 21.782454 9 AGTACTT 8730 0.0 21.248259 12-13 GTACTTT 9050 0.0 20.96707 14-15 CATGGGG 2695 0.0 20.62112 4 >>END_MODULE