FastQCFastQC Report
Thu 26 May 2016
SRR937864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976542
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61660.6314116545934533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47420.4855909935261361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32090.3286084981495932No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14410.14756149761095785No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.12165375375559884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.041.2936741
GTATCAA54100.036.480531
TAACGCG401.07887645E-535.6207476-77
ACGCGAA452.4153704E-531.66368778-79
AACGCGA452.4157942E-531.66287676-77
ATCAACG67500.029.0107123
TCAACGC69000.028.3800434
TATCAAC69200.028.298022
CAACGCA69800.027.9200155
AACGCAG71050.027.4133246
GTACATG55200.025.6736451
TACATGG55800.025.12772
ACGCAGA79450.024.2160367
CGCAGAG78850.023.9183258
ACATGGG55750.023.7861563
GAGTACT44700.022.84519612-13
GCAGAGT83200.022.2658189
GTATAGC6500.021.9491751
CGCGAAT652.9443958E-421.92101578-79
GTACTTT47350.021.8653214-15
Produced by FastQC (version 0.11.5)