##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937864_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 976542 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77520168103369 33.0 31.0 34.0 30.0 34.0 2 31.8607443407452 33.0 31.0 34.0 30.0 34.0 3 31.90057775292819 33.0 31.0 34.0 30.0 34.0 4 35.51441822266733 37.0 35.0 37.0 33.0 37.0 5 35.52032887474374 37.0 35.0 37.0 33.0 37.0 6 35.586233874221485 37.0 35.0 37.0 33.0 37.0 7 35.51438545397945 37.0 35.0 37.0 33.0 37.0 8 35.51538899504578 37.0 35.0 37.0 33.0 37.0 9 37.07635309080408 39.0 37.0 39.0 33.0 39.0 10-11 37.005294703146404 39.0 37.0 39.0 33.0 39.0 12-13 36.91686583884769 39.0 37.0 39.0 33.0 39.0 14-15 38.047732201994386 40.0 38.0 41.0 33.0 41.0 16-17 37.97446704801227 40.0 38.0 41.0 33.0 41.0 18-19 37.94009679051183 40.0 38.0 41.0 33.0 41.0 20-21 37.969314171843095 40.0 38.0 41.0 33.0 41.0 22-23 37.95540949595614 40.0 38.0 41.0 33.0 41.0 24-25 37.89069440945704 40.0 38.0 41.0 33.0 41.0 26-27 37.78966752070059 40.0 38.0 41.0 33.0 41.0 28-29 37.63444890235136 40.0 37.0 41.0 32.5 41.0 30-31 37.38426355446053 40.0 37.0 41.0 32.0 41.0 32-33 37.23620540642389 39.5 36.0 41.0 32.0 41.0 34-35 37.22274771592005 40.0 36.5 41.0 32.0 41.0 36-37 37.06570480327523 40.0 36.0 41.0 31.0 41.0 38-39 36.89317561354248 40.0 36.0 41.0 31.0 41.0 40-41 36.65721238820245 39.5 36.0 41.0 30.5 41.0 42-43 36.37768677640081 39.0 35.0 41.0 30.0 41.0 44-45 36.090136420143736 39.0 35.0 41.0 29.5 41.0 46-47 35.658509823438216 39.0 35.0 41.0 27.5 41.0 48-49 35.273214567320196 39.0 35.0 40.0 26.0 41.0 50-51 34.38654251430046 38.0 34.0 39.5 24.0 40.5 52-53 34.47260230486758 38.0 34.5 39.5 24.0 40.5 54-55 34.744444683382795 39.0 35.0 40.0 24.0 41.0 56-57 34.536805892629296 38.0 34.0 40.0 23.0 41.0 58-59 34.27943293785623 38.0 34.0 40.0 22.5 41.0 60-61 33.95386271148604 38.0 34.0 40.0 21.5 41.0 62-63 33.601460049849365 38.0 33.0 40.0 19.5 41.0 64-65 33.16980785260644 37.0 33.0 40.0 18.0 41.0 66-67 32.661471805616145 36.5 32.0 39.0 13.0 41.0 68-69 32.230050013209876 36.0 31.5 39.0 10.0 40.0 70-71 31.78948319683127 35.5 31.0 39.0 7.5 40.0 72-73 31.31284368721468 35.0 31.0 38.0 4.5 40.0 74-75 30.77325450415855 35.0 30.0 37.0 2.0 39.0 76-77 30.24067167617983 34.5 30.0 37.0 2.0 39.0 78-79 29.72830815264474 34.0 29.0 36.0 2.0 39.0 80-81 29.191380401457387 34.0 29.0 36.0 2.0 37.5 82-83 28.66635946021779 34.0 28.5 35.0 2.0 37.0 84-85 28.127922813355696 34.0 27.0 35.0 2.0 36.5 86-87 27.55499865853184 33.0 26.0 35.0 2.0 36.0 88-89 27.02449766625501 33.0 25.5 35.0 2.0 36.0 90-91 26.45983531686297 32.5 25.0 35.0 2.0 35.0 92-93 25.822335854474257 32.0 23.5 34.5 2.0 35.0 94-95 25.08217106893508 32.0 19.0 34.0 2.0 35.0 96-97 24.236182365940227 31.0 9.0 34.0 2.0 35.0 98-99 23.086183185157424 31.0 2.0 34.0 2.0 35.0 100-101 19.93945524104442 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.034878584042605354 1101 2 -0.11721266706197753 1101 3 -0.11325533522875375 1101 4 -0.03652818525683443 1101 5 -0.03888589420009936 1101 6 -0.03473040721662812 1101 7 -0.032129600775427036 1101 8 0.0013201701411631461 1101 9 0.1289946794761434 1101 10-11 0.07731043962471063 1101 12-13 0.10555998373613562 1101 14-15 0.3163784122820914 1101 16-17 0.38302145400894005 1101 18-19 0.3051638254539881 1101 20-21 0.12500733157476418 1101 22-23 0.11974932325707499 1101 24-25 0.11094677650023499 1101 26-27 0.09619839645275619 1101 28-29 0.049167461022967984 1101 30-31 0.09063447235147493 1101 32-33 0.24204701584091737 1101 34-35 0.3117481825016668 1101 36-37 0.4192748463747691 1101 38-39 0.431898635964707 1101 40-41 0.4209680684738686 1101 42-43 0.49663839487939043 1101 44-45 0.6032250052735719 1101 46-47 0.8257070568710105 1101 48-49 1.0769588925304348 1101 50-51 0.7221682019824414 1101 52-53 0.9222453380510203 1101 54-55 1.0272342769721874 1101 56-57 1.2768606059474195 1101 58-59 1.1937123077063987 1101 60-61 1.157914708105821 1101 62-63 1.189345513610398 1101 64-65 1.2978649900635162 1101 66-67 1.3340791046241591 1101 68-69 1.3439218149041494 1101 70-71 1.4608204789511916 1101 72-73 1.433875232853552 1101 74-75 1.5810838034071857 1101 76-77 1.2784058419261086 1101 78-79 1.4887091259380334 1101 80-81 1.6749118065631095 1101 82-83 1.6004136681999555 1101 84-85 1.6063669654245878 1101 86-87 1.9289196251915897 1101 88-89 1.8227633933088718 1101 90-91 1.836625188616015 1101 92-93 1.6736567529728283 1101 94-95 1.9425837634022152 1101 96-97 2.124377842857765 1101 98-99 2.3132600502939304 1101 100-101 3.0788626029635004 1102 1 0.0869241821825284 1102 2 0.03800407391295124 1102 3 -0.0060129337583276765 1102 4 0.1457944488637466 1102 5 0.09191583495767475 1102 6 0.09398650478612325 1102 7 0.09464616180263619 1102 8 0.02022087001447659 1102 9 0.15756471902074054 1102 10-11 0.12360589416520895 1102 12-13 0.18840154395461184 1102 14-15 0.278838840978743 1102 16-17 0.26816564387009834 1102 18-19 0.20698971368136654 1102 20-21 0.3419143201269037 1102 22-23 0.24303476662026924 1102 24-25 0.23433697304161427 1102 26-27 0.16908709669144883 1102 28-29 0.2384902453515494 1102 30-31 0.3234958822616747 1102 32-33 0.23762634451802 1102 34-35 0.4328623682620787 1102 36-37 0.46605013805405093 1102 38-39 0.5065149461108618 1102 40-41 0.49293023175670925 1102 42-43 0.5672883638240478 1102 44-45 0.5858908068817357 1102 46-47 0.5927645477260839 1102 48-49 0.8707906853053302 1102 50-51 0.5761326792095858 1102 52-53 0.6815216625465013 1102 54-55 0.6290816172552027 1102 56-57 0.7094482841395546 1102 58-59 0.5910332642500791 1102 60-61 0.7765471918810647 1102 62-63 0.8054776556570218 1102 64-65 0.9258862363189451 1102 66-67 1.0773279748485933 1102 68-69 1.1542906572757943 1102 70-71 0.9868490821280602 1102 72-73 1.0731316662356498 1102 74-75 1.0815563372546713 1102 76-77 0.945657098909745 1102 78-79 1.3719098772831977 1102 80-81 1.4891418009406614 1102 82-83 1.3903069158820287 1102 84-85 1.4528873177353603 1102 86-87 1.467314008589966 1102 88-89 1.5050659706294702 1102 90-91 1.6822406958361356 1102 92-93 1.7228791922590432 1102 94-95 1.9072565443407576 1102 96-97 2.138337048634643 1102 98-99 2.2738453862006374 1102 100-101 2.697280566182382 1103 1 0.08315450417045867 1103 2 -0.026355157275911978 1103 3 0.1554329992473491 1103 4 0.18506223050438564 1103 5 0.10480416349446386 1103 6 0.07621438811943193 1103 7 0.06134647551054684 1103 8 0.15615370723752164 1103 9 0.09615364244931612 1103 10-11 0.15440783835086336 1103 12-13 0.14527636011607825 1103 14-15 0.20301119503201193 1103 16-17 0.17415213926943096 1103 18-19 0.1996387638111372 1103 20-21 0.2129167471195217 1103 22-23 0.0823292412923422 1103 24-25 0.09620001705790315 1103 26-27 0.1206820053196509 1103 28-29 0.1945971833369029 1103 30-31 0.1457832677310762 1103 32-33 0.2769302783711325 1103 34-35 0.20426324705886856 1103 36-37 0.2948341668099701 1103 38-39 0.3869387174215504 1103 40-41 0.42788488599772734 1103 42-43 0.3976873209817313 1103 44-45 0.5415037942077277 1103 46-47 0.5138193889479084 1103 48-49 0.5703512869092364 1103 50-51 0.3537531639083866 1103 52-53 0.4273458228498157 1103 54-55 0.5394659839500733 1103 56-57 0.8044253294282981 1103 58-59 0.7469333960307196 1103 60-61 0.8375778279656387 1103 62-63 0.8444271941656112 1103 64-65 0.7924245777487329 1103 66-67 0.9589223522077219 1103 68-69 1.0009463184660703 1103 70-71 1.1815157885319252 1103 72-73 1.1122202417477745 1103 74-75 1.0010321431315425 1103 76-77 0.8788082890172539 1103 78-79 1.2407434306828655 1103 80-81 1.3598112129294613 1103 82-83 1.2528003560047658 1103 84-85 1.3407217742924509 1103 86-87 1.4946980785516253 1103 88-89 1.6811532905324924 1103 90-91 1.6176614415046728 1103 92-93 1.626682301349316 1103 94-95 1.7136569113051507 1103 96-97 1.9362332231085908 1103 98-99 2.0856634019236076 1103 100-101 2.445569296669582 1104 1 0.19005064262843518 1104 2 0.07068461721436847 1104 3 0.10321147567767497 1104 4 0.1473139785494837 1104 5 0.02339049204978494 1104 6 0.1059000437245885 1104 7 0.10518460628845361 1104 8 0.10677042772596934 1104 9 0.20839201029480847 1104 10-11 0.13297243023835392 1104 12-13 0.20759870307932005 1104 14-15 0.35205758902717577 1104 16-17 0.20814096557146655 1104 18-19 0.12373937634517773 1104 20-21 0.08340692211282885 1104 22-23 0.005158816951215783 1104 24-25 0.09129252102682273 1104 26-27 0.06943255735549059 1104 28-29 -0.002180733259308454 1104 30-31 0.15282108854803766 1104 32-33 0.050948393355476185 1104 34-35 0.30252037345168503 1104 36-37 0.2380067352665165 1104 38-39 0.3646065896743025 1104 40-41 0.5314826000357726 1104 42-43 0.3992967209413436 1104 44-45 0.4109125535595055 1104 46-47 0.4288851653524546 1104 48-49 0.5289283232032389 1104 50-51 0.4158668414275155 1104 52-53 0.4723957236064962 1104 54-55 0.6033739216098368 1104 56-57 0.8631880467744466 1104 58-59 0.8377902049105757 1104 60-61 0.8323042234055364 1104 62-63 0.7959061577008626 1104 64-65 1.1261761722909185 1104 66-67 1.1182296354690067 1104 68-69 1.1803091254513518 1104 70-71 1.2665678892232677 1104 72-73 1.1049043097881395 1104 74-75 1.2300757133299811 1104 76-77 1.127940874482256 1104 78-79 1.3179652415574417 1104 80-81 1.631163959318041 1104 82-83 1.7347827269761353 1104 84-85 1.7026793221205097 1104 86-87 1.7908706847682474 1104 88-89 1.97394210173049 1104 90-91 1.7078282261031603 1104 92-93 1.9520089670199923 1104 94-95 2.066473894800712 1104 96-97 2.068200572138071 1104 98-99 2.12276924012334 1104 100-101 2.649442166714188 1105 1 -0.011849192550133836 1105 2 0.047412508891117255 1105 3 -0.02849424389798827 1105 4 0.04146587194271234 1105 5 -0.07436386088366476 1105 6 0.04011416991179573 1105 7 -0.018592129713404404 1105 8 0.06871828748557363 1105 9 0.004179234093157902 1105 10-11 -0.022958343734543973 1105 12-13 -0.03245926930397758 1105 14-15 0.1684125869765225 1105 16-17 0.005606175211902098 1105 18-19 -0.08253225614665638 1105 20-21 0.012375610147600469 1105 22-23 0.0664283987996015 1105 24-25 0.024527009410526546 1105 26-27 0.14334323964229867 1105 28-29 0.046595322616745705 1105 30-31 0.1297093872531292 1105 32-33 0.18903574614673602 1105 34-35 0.12858115154639904 1105 36-37 0.07247001848269718 1105 38-39 0.09769853395633987 1105 40-41 0.3780888236231519 1105 42-43 0.5282698747077816 1105 44-45 0.7447306155448885 1105 46-47 0.8070789976123862 1105 48-49 0.9443412057611695 1105 50-51 1.0405398035781275 1105 52-53 0.926466699995089 1105 54-55 1.1246577527353665 1105 56-57 1.2426887471756984 1105 58-59 1.1501044856531593 1105 60-61 1.4983319140216977 1105 62-63 1.6166696540915808 1105 64-65 1.54478384955096 1105 66-67 1.8089815671546035 1105 68-69 1.6786945324668636 1105 70-71 1.6764797089883885 1105 72-73 1.4209307941434517 1105 74-75 1.6864344789196188 1105 76-77 1.5920931117881558 1105 78-79 1.8029213721600748 1105 80-81 1.7750639495139637 1105 82-83 1.676839601918708 1105 84-85 1.9643868536539628 1105 86-87 1.6676347129403375 1105 88-89 1.62514920953274 1105 90-91 1.5229959553915329 1105 92-93 1.829494955145254 1105 94-95 1.8360262282995023 1105 96-97 1.3936782386033535 1105 98-99 1.6335398662856484 1105 100-101 2.0722753500776427 1106 1 -0.37037088926496153 1106 2 -0.24230765723804382 1106 3 -0.26471210609792095 1106 4 -0.1845171457546826 1106 5 -0.4031130143731829 1106 6 -0.2722020248400767 1106 7 -0.2562070301921864 1106 8 -0.08687199670516321 1106 9 -0.3105502850888868 1106 10-11 -0.6813701716762495 1106 12-13 -0.625408724932619 1106 14-15 -0.8989033473105863 1106 16-17 -0.7878599477937698 1106 18-19 -0.5022939545663476 1106 20-21 -0.5133824607082857 1106 22-23 -0.5178797831089881 1106 24-25 -0.6866251446330693 1106 26-27 -0.6741287367169733 1106 28-29 -0.483172011313286 1106 30-31 -0.546526754837501 1106 32-33 -0.35605165162083807 1106 34-35 -0.43088768976426195 1106 36-37 -0.45981814078913175 1106 38-39 -0.70998801801111 1106 40-41 -0.46546419989918064 1106 42-43 -0.4405671738002326 1106 44-45 -0.39765647297998186 1106 46-47 -0.17217143215447095 1106 48-49 -0.1078526179089252 1106 50-51 -0.024529503727670487 1106 52-53 0.004148658311258657 1106 54-55 0.07582108308519508 1106 56-57 -0.09916746680320188 1106 58-59 -0.12322155466678453 1106 60-61 -0.013774014629895248 1106 62-63 0.1464038379607615 1106 64-65 0.3468860369814024 1106 66-67 0.5736691751882859 1106 68-69 0.075422621460568 1106 70-71 0.09887541652907927 1106 72-73 0.2577103130887082 1106 74-75 0.6130601342252859 1106 76-77 1.1065252275671114 1106 78-79 1.0215692285350677 1106 80-81 0.6791792927828446 1106 82-83 0.788099730446234 1106 84-85 0.7849607285645774 1106 86-87 1.0107747814188919 1106 88-89 1.0641829326160739 1106 90-91 1.3571973467469434 1106 92-93 1.5850364613893042 1106 94-95 1.4145293609802536 1106 96-97 1.379670917175627 1106 98-99 1.2992030332530327 1106 100-101 1.3625472279338773 1107 1 -0.08848242387961847 1107 2 -0.04133585176133181 1107 3 -0.005595516472858719 1107 4 0.0706703221446503 1107 5 0.11427541046394651 1107 6 0.1424034410588746 1107 7 0.16997495018247832 1107 8 0.0920010333275485 1107 9 0.34187179476405305 1107 10-11 -0.0062640705219791926 1107 12-13 0.05220325571236373 1107 14-15 0.2693041514053931 1107 16-17 0.1542960006289107 1107 18-19 0.2618445310930966 1107 20-21 0.2822187341123481 1107 22-23 0.18957297822424124 1107 24-25 0.31161235975964985 1107 26-27 0.34469171580106917 1107 28-29 0.23422992959445565 1107 30-31 0.25178914276474984 1107 32-33 0.32636394051821327 1107 34-35 0.21025725657693783 1107 36-37 0.4421599361443427 1107 38-39 0.5606598072531241 1107 40-41 0.6015755103162661 1107 42-43 0.6356681950533343 1107 44-45 0.7237529828630187 1107 46-47 0.7038828213529484 1107 48-49 0.6637771498849858 1107 50-51 0.5625221006299128 1107 52-53 0.8436365343959267 1107 54-55 0.9043379646573939 1107 56-57 1.0626327386410637 1107 58-59 0.8376433386914144 1107 60-61 1.0430279479035534 1107 62-63 1.2066422614462837 1107 64-65 1.1317885861123145 1107 66-67 1.0975628848090224 1107 68-69 1.082234721046973 1107 70-71 1.035829705178287 1107 72-73 1.238950682888401 1107 74-75 0.8260943569160659 1107 76-77 1.1608429363228225 1107 78-79 1.2900943549103268 1107 80-81 1.2672547394889797 1107 82-83 1.1886579441623404 1107 84-85 1.5130113057007932 1107 86-87 1.654493863407378 1107 88-89 1.7070268490326903 1107 90-91 1.9741073026845548 1107 92-93 2.0983038362980686 1107 94-95 2.2603204565929396 1107 96-97 2.253475420730357 1107 98-99 2.1353125674892404 1107 100-101 2.924817792635306 1108 1 0.09109167140982066 1108 2 -0.055524813281650154 1108 3 0.18851459876401933 1108 4 0.008122306779732469 1108 5 0.10642784661193616 1108 6 0.14937830459415125 1108 7 0.19936709251012985 1108 8 3.40544076244953E-4 1108 9 0.1235787831055859 1108 10-11 0.21092711486787152 1108 12-13 0.1997603687253715 1108 14-15 0.2092529350161456 1108 16-17 0.26849001639816095 1108 18-19 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-1.191122133958963 2316 86-87 -1.5498655198993738 2316 88-89 -1.4821212105101047 2316 90-91 -1.4071858924244331 2316 92-93 -1.3852554406822613 2316 94-95 -1.2056683791327423 2316 96-97 -1.2391614745381183 2316 98-99 -2.194817682377849 2316 100-101 -1.9938557852487975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3873.0 3 670.0 4 639.0 5 698.0 6 795.0 7 945.0 8 1224.0 9 1513.0 10 2070.0 11 2524.0 12 3782.0 13 5021.0 14 5643.0 15 7378.0 16 9634.0 17 12802.0 18 11099.0 19 7278.0 20 7125.0 21 7334.0 22 7940.0 23 8775.0 24 9683.0 25 11013.0 26 12624.0 27 14450.0 28 17166.0 29 20554.0 30 25262.0 31 30664.0 32 38589.0 33 50232.0 34 67667.0 35 94672.0 36 135780.0 37 187344.0 38 138772.0 39 13306.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72842312055105 19.51926712587746 14.756325108089891 21.995984645481595 2 14.683397929234113 20.418671809691645 42.11652730794273 22.781402953131515 3 18.852397565206317 25.74967880811365 30.440774886337614 24.95714874034242 4 11.819954671346725 16.775401349590307 38.72642299006857 32.6782209889944 5 12.571975489053358 38.501841812585944 34.740580158127855 14.185602540232848 6 30.241870928280264 36.88777960681606 18.429419419460512 14.440930045443162 7 27.28585311390758 33.98899724264046 21.146802971421767 17.578346672030197 8 25.51663059462932 35.516127741192626 20.320820492611645 18.64642117156641 9 26.526433969467217 16.63252697993079 21.261499053803874 35.57953999679812 10-11 24.103293009890574 27.55670724667428 27.799725475607097 20.540274267828053 12-13 25.018101393460917 25.1831306815412 28.634069619165153 21.164698305832726 14-15 22.173754867616733 26.155848913897383 27.626203979887613 24.04419223859827 16-17 20.524912109314876 29.642300817029344 28.301211075149872 21.531575998505907 18-19 20.353604097009484 27.78979739155284 31.485242895672886 20.371355615764784 20-21 21.63068299292366 26.824634678024655 31.417099885028044 20.127582444023638 22-23 21.0188301693176 27.002873268342743 31.274653668547973 20.703642893791688 24-25 20.457746352410716 27.049548313081345 31.70217754807912 20.790527786428818 26-27 20.51775289108543 27.390783212463095 31.35076724466141 20.740696651790067 28-29 20.28765969762326 27.368466425590416 31.708198183358554 20.635675693427775 30-31 20.525301912101423 27.313359462957205 31.614176501196155 20.54716212374522 32-33 20.324909969591324 27.269335883981107 31.46425518245718 20.94149896397039 34-35 20.54982063146141 27.447671133903707 31.345441073163173 20.65706716147171 36-37 20.67781565938365 27.23740479360511 31.20342935422551 20.881350192785735 38-39 20.38654198166252 27.584710285897213 31.351988885879155 20.676758846561107 40-41 20.660683029329263 27.430493568000262 31.223311151193545 20.68551225147693 42-43 20.38102479488095 27.480770730465977 31.110064480645544 21.028139994007528 44-45 20.60925592052809 27.870887902205467 30.60583889924713 20.91401727801932 46-47 20.593209294402445 28.050989297870967 30.085101885212506 21.270699522514082 48-49 20.741233744075053 27.934610424831085 30.106514377670624 21.217641453423244 50-51 20.961524843984275 28.33123465114155 29.614406914465103 21.092833590409075 52-53 20.907741188195743 28.46433067443449 29.556013673750392 21.071914463619375 54-55 20.79589302573786 28.268236115102006 29.653236730840277 21.282634128319856 56-57 20.839640813478987 28.63612230398506 29.474634567983564 21.04960231455239 58-59 20.9279305431751 28.27673998516739 29.52192015624503 21.27340931541248 60-61 20.93396779081781 28.283373910295673 29.723163546190534 21.059494752695983 62-63 20.951796203853597 28.12886626493417 29.822995594894426 21.09634193631781 64-65 20.85751571491447 28.041645164566724 29.75363979682183 21.347199323696973 66-67 20.66930779817761 28.28353297421742 29.646914949443858 21.40024427816111 68-69 20.75396593344475 28.256743600081663 29.658811230493498 21.330479235980093 70-71 20.74516299639017 28.25835039414712 29.51319868328649 21.483287926176224 72-73 20.733800083417854 28.07564860459783 29.502883969663813 21.687667342320506 74-75 20.68781524181173 28.23627737739335 29.32832041881916 21.747586961975767 76-77 20.710694276273934 28.17216327703327 29.432604100396087 21.684538346296716 78-79 20.680401031411254 28.138996313310148 29.341146557464853 21.83945609781374 80-81 20.548543273416414 28.40341083696431 29.274999628052345 21.773046261566932 82-83 20.725930804814915 28.369040987113237 29.23246754712374 21.672560660948108 84-85 20.72117313099956 28.065450024057228 29.14864084353158 22.06473600141163 86-87 20.669856312066123 28.026149141827396 29.3145237615245 21.98947078458198 88-89 20.91038093029651 27.74631802949681 29.315605973236586 22.027695066970104 90-91 20.66901484317047 27.961939430999827 29.517461014490227 21.851584711339473 92-93 20.65200663989642 27.936348508382974 29.4405846227714 21.97106022894921 94-95 20.41714566241457 28.169566803170838 29.218561078818972 22.19472645559562 96-97 20.610647077202007 28.145234148586272 29.10510970849231 22.13900906571941 98-99 20.759367166666152 28.060098440284793 28.881460207818378 22.299074185230676 100-101 21.107879107447225 27.56248849122307 29.208365412579717 22.121266988749994 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1451.0 1 876.0 2 325.0 3 365.0 4 345.5 5 327.0 6 565.5 7 1096.0 8 1694.0 9 1964.0 10 1824.0 11 1694.0 12 1753.0 13 1889.0 14 1999.5 15 2082.5 16 2189.0 17 2270.0 18 2408.0 19 2590.0 20 2768.5 21 3133.5 22 3673.0 23 4320.0 24 5152.5 25 6269.5 26 7688.0 27 9382.5 28 10715.5 29 12107.5 30 13983.5 31 15987.5 32 18291.0 33 20991.5 34 23863.5 35 26269.5 36 29024.5 37 31563.0 38 32580.5 39 32975.5 40 33423.0 41 34425.0 42 35096.5 43 35602.5 44 36873.5 45 37892.0 46 37900.5 47 37559.5 48 38491.0 49 37524.5 50 34202.0 51 31102.0 52 28383.5 53 26451.0 54 24467.0 55 21901.0 56 18895.5 57 16379.0 58 14328.0 59 12203.0 60 10104.0 61 8064.5 62 6262.0 63 4807.0 64 3636.5 65 2718.5 66 1997.0 67 1612.5 68 1290.5 69 875.0 70 609.5 71 442.0 72 342.0 73 272.0 74 198.5 75 144.5 76 101.5 77 72.0 78 48.5 79 32.5 80 23.5 81 14.0 82 8.5 83 7.5 84 8.0 85 6.0 86 2.0 87 4.0 88 3.5 89 0.5 90 1.0 91 1.0 92 0.5 93 1.5 94 1.5 95 1.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33567424647378197 2 0.28631641035408617 3 0.28928607269323797 4 0.2831419437156825 5 0.28396116091268986 6 0.21893579590022755 7 0.21084602607977948 8 0.21535172066332017 9 0.2164781443092053 10-11 0.21806537762840716 12-13 0.2221614636134442 14-15 0.21683655183289607 16-17 0.20849077663838317 18-19 0.20250025088526658 20-21 0.20034980574312214 22-23 0.20910518953613874 24-25 0.20772276051618876 26-27 0.21309887337154979 28-29 0.22134224641643677 30-31 0.22211026253863123 32-33 0.22006221954611271 34-35 0.22031822492017752 36-37 0.20628913042142583 38-39 0.19722654017953145 40-41 0.1929256498952426 42-43 0.20337066915708693 44-45 0.20572591859848322 46-47 0.21125563467828318 48-49 0.2218542571645664 50-51 0.2171949593565868 52-53 0.2320944721271589 54-55 0.21524931851369422 56-57 0.18836875423688892 58-59 0.1702947748279132 60-61 0.18954637895758708 62-63 0.18063739194013162 64-65 0.18749833596506857 66-67 0.18775434133913338 68-69 0.1846310757755427 70-71 0.18867596068576672 72-73 0.1976873498528481 74-75 0.20403628312965547 76-77 0.20500910355110172 78-79 0.20019620251868325 80-81 0.1989161756483592 82-83 0.18647431446880933 84-85 0.18258303278302418 86-87 0.18693512414212599 88-89 0.1974313444787833 90-91 0.1930280520448685 92-93 0.18719112951619082 94-95 0.18908556928427042 96-97 0.19830176275060363 98-99 0.19876257242392031 100-101 0.17843574572317422 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 976542.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.03671525279852 #Duplication Level Percentage of deduplicated Percentage of total 1 80.39207224084939 51.4804423866968 2 11.856954777043892 15.185608736457375 3 3.5573657146446656 6.834060459563056 4 1.4655745151140345 3.7540231162446274 5 0.7764195485077188 2.4859678772247586 6 0.43328911403134934 1.664784697041772 7 0.3007293104674175 1.348040205580072 8 0.19782236871524 1.0134315756841552 9 0.1501301929843182 0.8652459977086028 >10 0.803947899482719 9.472436711810635 >50 0.04567076591312124 1.983580755184876 >100 0.018581856978381536 1.9023348052602536 >500 6.40753452343269E-4 0.2922486135556065 >1k 6.40753452343269E-4 1.0852896916175208 >5k 1.6018836308581726E-4 0.6325043703699086 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6166 0.6314116545934533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4742 0.4855909935261361 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3209 0.3286084981495932 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1441 0.14756149761095785 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1188 0.12165375375559884 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.192171970073996E-4 0.0 0.0 0.0 0.0 7 8.192171970073996E-4 0.0 0.0 0.0 0.0 8 8.192171970073996E-4 0.0 0.0 0.0 0.0 9 8.192171970073996E-4 0.0 0.0 0.0 0.0 10-11 8.192171970073996E-4 0.0 0.0 0.0 0.0 12-13 8.70418271820362E-4 0.0 0.0 1.0240214962592494E-4 0.0 14-15 9.216193466333245E-4 0.0 0.0 2.5600537406481234E-4 0.0 16-17 0.0011264236458851744 0.0 0.0 3.072064488777748E-4 0.0 18-19 0.0011264236458851744 0.0 0.0 3.072064488777748E-4 0.0 20-21 0.0011264236458851744 0.0 0.0 4.096085985036998E-4 0.0 22-23 0.0011264236458851744 0.0 0.0 5.632118229425872E-4 0.0 24-25 0.0011264236458851744 0.0 0.0 6.656139725685121E-4 0.0 26-27 0.0011264236458851744 0.0 0.0 0.0012800268703240618 0.0 28-29 0.0011264236458851744 0.0 0.0 0.0032768687880295987 0.0 30-31 0.0011264236458851744 0.0 0.0 0.006963346174562897 0.0 32-33 0.0011264236458851744 0.0 0.0 0.00972820421446287 0.0 34-35 0.0011264236458851744 0.0 0.0 0.012800268703240619 0.0 36-37 0.0011264236458851744 0.0 0.0 0.018176381558601677 0.0 38-39 0.0011264236458851744 0.0 0.0 0.023757298713214588 0.0 40-41 0.0011264236458851744 0.0 0.0 0.03210307390772747 0.0 42-43 0.0011264236458851744 0.0 0.0 0.04510814691021994 0.0 44-45 0.0012288257955110995 0.0 0.0 0.05719160056607908 0.0 46-47 0.0013312279451370243 0.0 0.0 0.08222892614961773 0.0 48-49 0.0013312279451370243 0.0 0.0 0.11279596781295634 0.0 50-51 0.0014336300947629492 0.0 0.0 0.12815629025684508 0.0 52-53 0.0014336300947629492 0.0 0.0 0.15682889215210405 0.0 54-55 0.0014336300947629492 0.0 0.0 0.20669873901992952 0.0 56-57 0.0014336300947629492 0.0 0.0 0.2841659652119417 0.0 58-59 0.0014336300947629492 0.0 0.0 0.3905105975984648 0.0 60-61 0.0014848311695759117 0.0 0.0 0.517028453461295 0.0 62-63 0.001945640842892574 0.0 0.0 0.6422662824538012 0.0 64-65 0.001945640842892574 0.0 0.0 0.7758498866408203 0.0 66-67 0.001945640842892574 0.0 0.0 0.9455814496457909 0.0 68-69 0.001945640842892574 0.0 0.0 1.16661648961335 0.0 70-71 0.0022016462169573863 0.0 0.0 1.32907749999488 0.0 72-73 0.0026624558902740487 0.0 0.0 1.52558722512703 0.0 74-75 0.0026624558902740487 0.0 0.0 1.7372524684038169 0.0 76-77 0.0026624558902740487 0.0 0.0 1.97149738567312 0.0 78-79 0.0026624558902740487 0.0 0.0 2.2416854574611227 0.0 80-81 0.0026624558902740487 0.0 0.0 2.597942536009716 0.0 82-83 0.0026624558902740487 0.0 0.0 2.958090896244094 0.0 84-85 0.0026624558902740487 0.0 0.0 3.42571031251088 0.0 86-87 0.0026624558902740487 0.0 0.0 3.969824134548232 0.0 88-89 0.0026624558902740487 0.0 0.0 4.682799101318735 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3455 0.0 41.293674 1 GTATCAA 5410 0.0 36.48053 1 TAACGCG 40 1.07887645E-5 35.62074 76-77 ACGCGAA 45 2.4153704E-5 31.663687 78-79 AACGCGA 45 2.4157942E-5 31.662876 76-77 ATCAACG 6750 0.0 29.010712 3 TCAACGC 6900 0.0 28.380043 4 TATCAAC 6920 0.0 28.29802 2 CAACGCA 6980 0.0 27.920015 5 AACGCAG 7105 0.0 27.413324 6 GTACATG 5520 0.0 25.673645 1 TACATGG 5580 0.0 25.1277 2 ACGCAGA 7945 0.0 24.216036 7 CGCAGAG 7885 0.0 23.918325 8 ACATGGG 5575 0.0 23.786156 3 GAGTACT 4470 0.0 22.845196 12-13 GCAGAGT 8320 0.0 22.265818 9 GTATAGC 650 0.0 21.949175 1 CGCGAAT 65 2.9443958E-4 21.921015 78-79 GTACTTT 4735 0.0 21.86532 14-15 >>END_MODULE