FastQCFastQC Report
Thu 26 May 2016
SRR937864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976542
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78940.8083625691470515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64480.6602890607879641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45280.46367693350618816No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.1559584738802837No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13170.13486363105734317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCG352.7875646E-454.2863855
GGTATCA37050.042.588621
GTATCAA63600.040.203961
ATCAACG80250.031.785443
TATCAAC81400.031.5114442
TCAACGC81450.031.3171464
CAACGCA82650.030.8624525
AACGCAG83900.030.4010856
ACGCAGA96450.026.3495257
CGCAGAG96900.026.080098
GCAGAGT101750.024.3701029
TACCTGG7400.023.1083932
GTACATG56550.022.6921081
GTATAGC5500.022.4674781
TACATGG57250.022.3190542
GAGTACT62050.022.31608412-13
AGTACTT62650.022.2919212-13
GTACTTT63050.021.7338914-15
GTCGGGA1100.002876852421.5911772
GTACCGT1100.002877707421.590076
Produced by FastQC (version 0.11.5)