##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937864_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 976542 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16996606392762 33.0 31.0 34.0 31.0 34.0 2 32.4058320072255 34.0 31.0 34.0 31.0 34.0 3 32.48537902107641 34.0 31.0 34.0 31.0 34.0 4 36.117015960399044 37.0 35.0 37.0 35.0 37.0 5 35.95040459089317 37.0 35.0 37.0 35.0 37.0 6 36.05044432292723 37.0 35.0 37.0 35.0 37.0 7 36.00343661614144 37.0 35.0 37.0 35.0 37.0 8 36.005783673410875 37.0 35.0 37.0 35.0 37.0 9 37.67705434072472 39.0 37.0 39.0 35.0 39.0 10-11 37.61635085843722 39.0 37.0 39.0 35.0 39.0 12-13 37.5325771958605 39.0 37.0 39.0 35.0 39.0 14-15 38.72888160468265 40.0 38.0 41.0 34.5 41.0 16-17 38.695432454518084 40.0 38.0 41.0 34.5 41.0 18-19 38.7218993141104 40.0 38.0 41.0 34.5 41.0 20-21 38.67102695019774 40.0 38.0 41.0 34.0 41.0 22-23 38.5209412395985 40.0 38.0 41.0 34.0 41.0 24-25 38.45684005398641 40.0 38.0 41.0 34.0 41.0 26-27 38.36399048888834 40.0 38.0 41.0 34.0 41.0 28-29 38.240769982243464 40.0 38.0 41.0 34.0 41.0 30-31 38.275576984912064 40.0 38.0 41.0 34.0 41.0 32-33 38.15211429718333 40.0 37.0 41.0 34.0 41.0 34-35 38.00264965562157 40.0 37.0 41.0 33.5 41.0 36-37 37.831492654693804 40.0 37.0 41.0 33.0 41.0 38-39 37.68411752899517 40.0 37.0 41.0 33.0 41.0 40-41 37.48223732312589 40.0 37.0 41.0 33.0 41.0 42-43 37.25063642935993 40.0 36.5 41.0 32.5 41.0 44-45 36.883764855991856 40.0 36.0 41.0 31.5 41.0 46-47 36.429687612002354 40.0 36.0 41.0 30.5 41.0 48-49 36.02340349928626 39.0 36.0 41.0 29.5 41.0 50-51 35.74707385857444 39.0 35.0 41.0 28.0 41.0 52-53 35.51740222130743 39.0 35.0 40.5 27.5 41.0 54-55 35.314564555339146 39.0 35.0 40.0 27.0 41.0 56-57 35.04898918838104 38.5 35.0 40.0 26.0 41.0 58-59 34.80249236591975 38.0 34.5 40.0 26.0 41.0 60-61 34.47752836027534 38.0 34.0 40.0 25.5 41.0 62-63 34.12362755518964 38.0 34.0 40.0 24.0 41.0 64-65 33.73998814183108 37.0 33.5 40.0 23.0 41.0 66-67 33.33188587894837 36.5 33.0 39.0 22.0 41.0 68-69 32.866894101840984 36.0 32.5 39.0 20.5 40.0 70-71 32.475523326185666 35.5 32.0 39.0 19.5 40.0 72-73 32.03224899697094 35.0 31.5 38.0 18.0 40.0 74-75 31.538658859526777 35.0 31.0 37.0 15.0 39.0 76-77 30.277972683202567 34.0 29.5 36.0 13.0 38.5 78-79 30.81238492558436 34.0 31.0 36.0 11.0 39.0 80-81 30.609066993534327 34.0 31.0 36.0 7.5 38.0 82-83 30.251575457071993 34.0 30.5 35.5 6.5 37.0 84-85 29.85172783147064 34.0 30.0 35.0 2.0 37.0 86-87 29.412475346682477 34.0 30.0 35.0 2.0 36.0 88-89 28.97346350694594 34.0 29.0 35.0 2.0 36.0 90-91 28.46950975994888 34.0 29.0 35.0 2.0 35.5 92-93 28.009957073018874 33.5 28.0 35.0 2.0 35.0 94-95 27.53552125766224 33.0 27.0 35.0 2.0 35.0 96-97 27.01655893960526 33.0 26.0 35.0 2.0 35.0 98-99 26.233398051491896 33.0 25.0 34.5 2.0 35.0 100-101 23.901153253009085 30.5 11.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1029525392117776 1101 2 0.10514711410760214 1101 3 0.08245888767667253 1101 4 0.020682464047204974 1101 5 -0.03215008506089134 1101 6 0.012344301850063744 1101 7 0.031634928917135596 1101 8 0.02518190870511461 1101 9 0.09328259507330472 1101 10-11 0.12349953328957497 1101 12-13 0.18284361169804697 1101 14-15 0.10027903255031134 1101 16-17 -0.11948069373282522 1101 18-19 0.05333734219492925 1101 20-21 0.06334621239754767 1101 22-23 0.029204764751177947 1101 24-25 0.011935162945611921 1101 26-27 0.05947963246962473 1101 28-29 0.07628754440366947 1101 30-31 0.11672204929782026 1101 32-33 0.19755773443057478 1101 34-35 0.2138997492191308 1101 36-37 0.39156694629802047 1101 38-39 0.5542762735962015 1101 40-41 0.4818788119218098 1101 42-43 0.5798869169003353 1101 44-45 0.6883764604896214 1101 46-47 0.7887389933600275 1101 48-49 0.9520368213981953 1101 50-51 1.1408630198937502 1101 52-53 1.0195814393282774 1101 54-55 1.1471216562704925 1101 56-57 1.1799835571679083 1101 58-59 1.2107072423211065 1101 60-61 1.1305636904370928 1101 62-63 1.0836586795025482 1101 64-65 1.1658693075680304 1101 66-67 1.2529461294406516 1101 68-69 1.2729136669537056 1101 70-71 1.3178970749593013 1101 72-73 1.2184603726957484 1101 74-75 1.2413667884147905 1101 76-77 1.1053145769668937 1101 78-79 1.1742468239782973 1101 80-81 1.2476361927687414 1101 82-83 1.1799552306669305 1101 84-85 1.3440840356470467 1101 86-87 1.524642915388931 1101 88-89 1.5672341997002022 1101 90-91 1.451357049376373 1101 92-93 1.785655716887991 1101 94-95 1.63324925782943 1101 96-97 1.709443348892151 1101 98-99 1.8520769668287649 1101 100-101 2.261313800024716 1102 1 0.14859963589186975 1102 2 0.20185337333345643 1102 3 0.2138413919992388 1102 4 0.05784444428994817 1102 5 -0.0019053027175033321 1102 6 -0.011559915593551295 1102 7 0.07039774701089385 1102 8 0.0804113595869893 1102 9 0.21911263593030839 1102 10-11 0.18589907215758217 1102 12-13 0.1715982871670363 1102 14-15 0.1754619472960499 1102 16-17 0.26312129673016216 1102 18-19 0.206944742979708 1102 20-21 0.18626016894361186 1102 22-23 0.2492342918489001 1102 24-25 0.18868309671369587 1102 26-27 0.18940365861932662 1102 28-29 0.06573090934171688 1102 30-31 0.2905888891505981 1102 32-33 0.4286094541594707 1102 34-35 0.5500083332461045 1102 36-37 0.4536784956792772 1102 38-39 0.44350125922397865 1102 40-41 0.5004942474170733 1102 42-43 0.7235553012575124 1102 44-45 0.8193821367820249 1102 46-47 0.8837941637305491 1102 48-49 0.8930608910842395 1102 50-51 0.8971054263783813 1102 52-53 0.9120507213580566 1102 54-55 0.9966764716138528 1102 56-57 1.0076703562835831 1102 58-59 1.1717508325057509 1102 60-61 1.0245652465831583 1102 62-63 1.1418725604214046 1102 64-65 1.2657903230176544 1102 66-67 1.2620538787123436 1102 68-69 1.3069152841422067 1102 70-71 1.3320124839665048 1102 72-73 1.2166402180970586 1102 74-75 1.1346683186396227 1102 76-77 1.1155857296420884 1102 78-79 1.2868320735535583 1102 80-81 1.2105434302868154 1102 82-83 1.289704405122503 1102 84-85 1.4135665680273313 1102 86-87 1.2194941822265584 1102 88-89 1.2954944270876325 1102 90-91 1.568954972887152 1102 92-93 1.73144011544451 1102 94-95 1.5785915347514852 1102 96-97 1.7574606361096698 1102 98-99 2.0372428703559926 1102 100-101 2.6279132982123024 1103 1 0.19633791978910864 1103 2 0.22420373362459145 1103 3 0.25985292183388964 1103 4 0.07365190201194594 1103 5 -0.052761229024000045 1103 6 0.030338814155058458 1103 7 0.12357933607556504 1103 8 0.05703085560701027 1103 9 0.1445126102738996 1103 10-11 0.11242365242262053 1103 12-13 0.1468250807508582 1103 14-15 0.16358899057634346 1103 16-17 0.19000571460827587 1103 18-19 0.11730526735524194 1103 20-21 0.174319183867091 1103 22-23 0.19497124627767448 1103 24-25 0.2076434420126816 1103 26-27 0.2666534211679661 1103 28-29 0.1702000484396322 1103 30-31 0.3144871861092753 1103 32-33 0.4769005170474685 1103 34-35 0.3601422260100975 1103 36-37 0.3950416802874983 1103 38-39 0.41482944486235596 1103 40-41 0.46775446689598965 1103 42-43 0.5536857731669826 1103 44-45 0.5890788080573515 1103 46-47 0.8687387756903249 1103 48-49 1.045524748590097 1103 50-51 1.0591018059740165 1103 52-53 1.0806229162897552 1103 54-55 1.0866498692868944 1103 56-57 1.13127101298398 1103 58-59 1.0984128948404575 1103 60-61 1.0791927945690603 1103 62-63 1.2377361771716622 1103 64-65 1.2720618896264853 1103 66-67 1.183056500071693 1103 68-69 1.0360140574289645 1103 70-71 1.1935440679128497 1103 72-73 1.2409891867223841 1103 74-75 1.1946773631716319 1103 76-77 1.206272323650154 1103 78-79 1.3330124432909152 1103 80-81 1.3489396714933442 1103 82-83 1.3250135842858697 1103 84-85 1.3303071156491235 1103 86-87 1.461835290661167 1103 88-89 1.5179522073936198 1103 90-91 1.5870760426298212 1103 92-93 1.5927908087506495 1103 94-95 1.6511299772519727 1103 96-97 1.9210092284409974 1103 98-99 2.0469980071448184 1103 100-101 2.562784763491276 1104 1 -0.014609217751136327 1104 2 0.10816670248566851 1104 3 0.06680602874907038 1104 4 0.03457942073190878 1104 5 -0.1024209779359353 1104 6 -0.016550330480797015 1104 7 0.07192290019955294 1104 8 0.0049469100406156485 1104 9 0.0018406090393128238 1104 10-11 0.010149511012791379 1104 12-13 0.14371118816348627 1104 14-15 0.09879451208398393 1104 16-17 -0.08685770392601455 1104 18-19 0.0039638128700900666 1104 20-21 0.027855414656656308 1104 22-23 0.017970249740109523 1104 24-25 -0.06025901879482376 1104 26-27 -0.016716222032179928 1104 28-29 -0.027631374179314605 1104 30-31 0.04530071866256691 1104 32-33 0.09978903125428928 1104 34-35 0.11089192762343458 1104 36-37 0.20306930752663277 1104 38-39 0.02853602283115464 1104 40-41 0.07257971163871701 1104 42-43 0.4263886884087569 1104 44-45 0.5274292510815002 1104 46-47 0.46351590096675466 1104 48-49 0.6020244883739494 1104 50-51 0.5543396041349453 1104 52-53 0.5451895209532864 1104 54-55 0.8740613178125258 1104 56-57 0.9818266606595287 1104 58-59 0.88500560648977 1104 60-61 0.9100215953255812 1104 62-63 1.0720219946526086 1104 64-65 0.8205844036592822 1104 66-67 0.9341780563120281 1104 68-69 0.9674984058789136 1104 70-71 1.0149543573684277 1104 72-73 0.9701887224376655 1104 74-75 0.9733501458755498 1104 76-77 0.8517710767468891 1104 78-79 0.9503441523938854 1104 80-81 1.2123130578658952 1104 82-83 1.24391266474602 1104 84-85 1.1046452062043457 1104 86-87 1.1602001153477168 1104 88-89 1.2729487679435358 1104 90-91 1.14527597072151 1104 92-93 1.2797534049362262 1104 94-95 1.3140774956764254 1104 96-97 1.695346826473532 1104 98-99 1.885207917029586 1104 100-101 2.2177557128615852 1105 1 -0.06764568429998263 1105 2 -0.025211869907181494 1105 3 -0.0013728612753567404 1105 4 -0.00284751707481945 1105 5 -0.09883248022347146 1105 6 -0.07963828640248494 1105 7 -0.059408108557896355 1105 8 0.0014636393387377211 1105 9 -0.04346911511706253 1105 10-11 -0.05214693246824709 1105 12-13 -0.07773597581458347 1105 14-15 -0.23067895762013535 1105 16-17 -0.012176811251919162 1105 18-19 0.02137862647198574 1105 20-21 -0.03923193762130239 1105 22-23 -0.09718069455681899 1105 24-25 -0.13739272742071762 1105 26-27 -0.24150857834213468 1105 28-29 -0.14239734160821627 1105 30-31 -0.1466610669936017 1105 32-33 -0.07062200598119261 1105 34-35 -0.06075154889220613 1105 36-37 0.01984341695964531 1105 38-39 0.04443197436308566 1105 40-41 -0.13070527031100454 1105 42-43 -0.12652047912239794 1105 44-45 -0.1382701825918602 1105 46-47 -0.37971433213930794 1105 48-49 -0.3919501103390033 1105 50-51 -0.06095891462806691 1105 52-53 -0.05292825014358016 1105 54-55 -0.1245735542134625 1105 56-57 8.961048401872063E-4 1105 58-59 0.10789335390798271 1105 60-61 0.12886323586086945 1105 62-63 0.1020496770559376 1105 64-65 0.07991893777823833 1105 66-67 0.3001142250941058 1105 68-69 0.17608605920878517 1105 70-71 0.3892689060549195 1105 72-73 0.5664006013865652 1105 74-75 0.6866594121661045 1105 76-77 0.350240152068384 1105 78-79 0.5244054889617082 1105 80-81 0.7400763063315097 1105 82-83 0.616027968911105 1105 84-85 0.6066606884340686 1105 86-87 0.572584769861777 1105 88-89 0.5912645814073585 1105 90-91 0.5905561286544234 1105 92-93 0.7199559746311515 1105 94-95 0.6827898609794367 1105 96-97 1.0519147114978828 1105 98-99 1.5220371654161475 1105 100-101 2.1194854909249905 1106 1 -0.2744547067994034 1106 2 -0.1504831084408096 1106 3 -0.2311302501871353 1106 4 -0.08327998329727393 1106 5 -0.16979065905383806 1106 6 -0.22959969026020843 1106 7 -0.10531147139478492 1106 8 -0.16755526093167106 1106 9 -0.2535183192788182 1106 10-11 -0.26470180765826967 1106 12-13 -0.3684549059200535 1106 14-15 -0.25284971831931813 1106 16-17 -0.24667655315501236 1106 18-19 -0.11578874818526685 1106 20-21 -0.3662800194167133 1106 22-23 -0.16438865436000327 1106 24-25 -0.11323627807637848 1106 26-27 -0.43225450206858085 1106 28-29 -0.3328368640579171 1106 30-31 -0.3018379225682679 1106 32-33 -0.1426523524940393 1106 34-35 -0.28533421330467235 1106 36-37 -0.0264227022574417 1106 38-39 -0.1759401587486238 1106 40-41 -0.2998861323073001 1106 42-43 -0.4074023654555816 1106 44-45 -0.09811314601787302 1106 46-47 -0.05304368746534038 1106 48-49 -0.07529741405512169 1106 50-51 0.02616228673014831 1106 52-53 -0.03898296668687351 1106 54-55 -0.1465956132110975 1106 56-57 -0.2580323904785473 1106 58-59 -0.312351861827878 1106 60-61 -0.17372673103642455 1106 62-63 -0.19939140344661865 1106 64-65 -0.3390694170789601 1106 66-67 -0.23056189407562755 1106 68-69 -0.3291833797501127 1106 70-71 -0.07211079947783006 1106 72-73 0.08524783169898242 1106 74-75 -0.02906508241386163 1106 76-77 0.06266953059945735 1106 78-79 0.36289081532406087 1106 80-81 0.13667084793720008 1106 82-83 0.1671183691270599 1106 84-85 -0.02785468135485658 1106 86-87 -0.03374641818904678 1106 88-89 0.12309148126102443 1106 90-91 0.07993437558448946 1106 92-93 0.14831253668569744 1106 94-95 -0.2648088906839874 1106 96-97 -0.21357370729157665 1106 98-99 -0.25586168901246253 1106 100-101 0.4143638065230384 1107 1 0.0356512591941609 1107 2 0.07722078148430711 1107 3 0.03850589719209552 1107 4 0.11667946849090072 1107 5 -0.11896888196087474 1107 6 -0.06535240023236355 1107 7 0.032674093441805496 1107 8 -0.0655129826628098 1107 9 0.03342380490683894 1107 10-11 0.06877074120232862 1107 12-13 0.060582344370551766 1107 14-15 0.08182981564657155 1107 16-17 -0.01805289462502202 1107 18-19 0.03342056168416718 1107 20-21 -0.04473202384998132 1107 22-23 -0.05862945667395536 1107 24-25 -0.03455450044277342 1107 26-27 0.04666515840772689 1107 28-29 -0.019150490960292643 1107 30-31 0.005132990847783958 1107 32-33 0.10148843089741888 1107 34-35 0.28961712884114377 1107 36-37 -0.08662192913821087 1107 38-39 -0.09662109838584598 1107 40-41 0.028603717123672823 1107 42-43 0.24352889180931214 1107 44-45 -0.19512947343116593 1107 46-47 -0.045634853361583794 1107 48-49 -0.06616663255591249 1107 50-51 0.13573571954175634 1107 52-53 0.22091147280167434 1107 54-55 0.07592216042174726 1107 56-57 0.24848370716154733 1107 58-59 0.21895338511073703 1107 60-61 0.2295211807306643 1107 62-63 0.14634765529422822 1107 64-65 0.3958432691049154 1107 66-67 0.6162145230843734 1107 68-69 0.750266786497555 1107 70-71 0.6602189656128665 1107 72-73 0.6484381650052846 1107 74-75 0.44253900484588726 1107 76-77 0.44354158747508166 1107 78-79 0.7437953107691406 1107 80-81 0.7091061735393431 1107 82-83 0.5974727604983983 1107 84-85 0.44302021389559343 1107 86-87 0.5737739234972175 1107 88-89 0.8696874588784098 1107 90-91 0.7684619348524571 1107 92-93 0.8753189973674473 1107 94-95 0.7518043875037783 1107 96-97 0.7720424903674434 1107 98-99 0.9836518676063584 1107 100-101 1.3722402966698226 1108 1 -0.05147388400955322 1108 2 -0.023921478775818628 1108 3 0.02951270358066438 1108 4 0.07389356647593814 1108 5 -0.04259244149992725 1108 6 -0.06425394346303648 1108 7 0.03450710105687449 1108 8 -0.06480201831210763 1108 9 -0.05510978900110075 1108 10-11 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3.0 4 3.0 5 19.0 6 38.0 7 101.0 8 242.0 9 502.0 10 868.0 11 1468.0 12 2452.0 13 3509.0 14 4470.0 15 6462.0 16 9186.0 17 13454.0 18 12054.0 19 6820.0 20 5259.0 21 5253.0 22 5434.0 23 5876.0 24 6744.0 25 7599.0 26 9131.0 27 11193.0 28 13879.0 29 17015.0 30 21385.0 31 26770.0 32 34152.0 33 44427.0 34 60983.0 35 87613.0 36 134954.0 37 214478.0 38 185257.0 39 17487.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14277665377622 20.30619509821497 15.288754577816476 21.262273670192332 2 15.28771931980396 21.24434996139439 40.968028000843795 22.499902717957855 3 19.18698837325993 25.992635237398904 30.764165801368502 24.05621058797266 4 12.295323703435182 17.624536374267567 38.06349342885406 32.01664649344319 5 12.586066165589102 38.99638376164575 34.29271355111329 14.124836521651856 6 30.28809820775758 37.60503900497879 17.628325253803727 14.478537533459903 7 27.15766449369305 34.669681385951655 20.812315292122612 17.36033882823268 8 26.062371101294158 35.86594329788171 19.68681326558407 18.38487233524006 9 26.650057037997342 16.779821042003316 21.0935115949954 35.476610325003946 10-11 24.374732474384103 27.964695834894965 27.188026731057143 20.472544959663793 12-13 25.435858888684642 25.583120795214292 28.368500903489686 20.612519412611373 14-15 22.348603541885552 26.488005636214318 27.661022260179287 23.50236856172085 16-17 20.424467150414422 29.96773308265287 28.341126136919865 21.266673630012843 18-19 20.29083234515259 27.75876511199723 31.966981450874616 19.98342109197556 20-21 21.48316277393229 26.75022003673167 32.167245324443456 19.599371864892582 22-23 21.02051485018737 26.83755292300902 32.079855694078 20.062076532725605 24-25 20.536886429406742 26.949693713915895 32.41916853210014 20.094251324577233 26-27 20.591539343792242 27.256533404333755 32.11731454552902 20.03461270634498 28-29 20.243590354080037 27.330106557339278 32.372520075185626 20.053783013395062 30-31 20.569542317071985 27.154382546105456 32.33226339770526 19.9438117391173 32-33 20.330154770608942 27.17486805483021 32.24684652580227 20.24813064875858 34-35 20.692248771686213 27.299593873074585 31.969746308914516 20.038411046324683 36-37 20.79168125897299 27.14394260564318 31.9909947549619 20.073381380421935 38-39 20.575971130785977 27.468250213508483 31.99565405276988 19.960124602935664 40-41 20.664958598810905 27.421503632204246 31.879376411869636 20.034161357115206 42-43 20.599344337511535 27.385993894002254 31.717596068073373 20.297065700412837 44-45 20.69025192976851 27.756000253957332 31.24479029063778 20.30895752563638 46-47 20.79183486219743 27.971044768171772 30.645328106727614 20.591792262903184 48-49 20.96561131011262 28.044262305154312 30.396081274538115 20.59404511019495 50-51 21.104775831454255 28.394836064398664 29.900966881096767 20.599421223050314 52-53 21.129045140915597 28.59462265831884 29.731184117016983 20.545148083748575 54-55 21.031455892322093 28.469487231475966 29.791345379922213 20.707711496279728 56-57 21.134216449471708 28.856311351687896 29.512760331864886 20.496711866975513 58-59 21.12218419689066 28.44649794888494 29.72365755901948 20.707660295204917 60-61 21.21327090898292 28.351673558331335 29.90987586811422 20.525179664571517 62-63 21.142818230040287 28.294686762064508 29.99850492861546 20.563990079279744 64-65 21.143586246162478 28.113230152927372 29.887552199495772 20.855631401414378 66-67 20.98470931101786 28.427246344755268 29.75079412867035 20.837250215556523 68-69 21.09417720896797 28.439995412383695 29.645473517780086 20.82035386086825 70-71 20.999660024863243 28.468002400306386 29.586131472071862 20.94620610275851 72-73 20.994459014009653 28.328303675933732 29.464610292860204 21.21262701719641 74-75 21.01564552044127 28.3976154623229 29.274024708627334 21.312714308608495 76-77 20.921271179324595 28.430932822141802 29.5017521007801 21.146043897753504 78-79 20.917584701938065 28.365907457129342 29.31952747552077 21.39698036541183 80-81 20.776013730080223 28.692160705837537 29.150307923263924 21.381517640818316 82-83 20.81280807094022 28.72374468852034 29.10555168986847 21.357895550670968 84-85 20.841039095092682 28.42212623727397 29.082876107735256 21.65395855989809 86-87 20.726860698259777 28.34634864655079 29.08262010236119 21.844170552828245 88-89 20.88763207317248 28.110516495962283 29.211646810889853 21.79020461997538 90-91 20.78272335087113 28.319059871730868 29.350640705608257 21.547576071789752 92-93 20.558220077633255 28.245202708203514 29.2857402527049 21.91083696145833 94-95 20.280899336638875 28.437588961867487 29.2695040254285 22.01200767606514 96-97 20.550370593379498 28.45448531655577 28.972332987214067 22.02281110285067 98-99 20.592745513370925 28.502248498654176 28.73403612036572 22.170969867609177 100-101 20.457350264797363 28.35364760801592 28.810065884410907 22.37893624277581 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 602.0 1 421.0 2 304.5 3 393.0 4 353.5 5 338.5 6 613.0 7 1278.0 8 2045.0 9 2442.5 10 2270.5 11 2012.5 12 2030.0 13 2187.5 14 2339.5 15 2471.0 16 2574.5 17 2654.5 18 2800.0 19 2932.0 20 3164.0 21 3461.0 22 3897.5 23 4674.0 24 5459.0 25 6386.0 26 7824.0 27 9586.0 28 10846.0 29 12273.0 30 14402.5 31 16352.5 32 18337.5 33 20794.5 34 23566.0 35 26271.0 36 29334.5 37 31451.5 38 32409.5 39 33031.5 40 33324.0 41 34189.0 42 34773.0 43 35412.5 44 36987.0 45 38039.5 46 37883.0 47 37395.5 48 38417.5 49 37165.5 50 33961.5 51 31401.5 52 28246.5 53 25940.5 54 24002.0 55 21355.0 56 18404.5 57 15997.0 58 13830.0 59 11858.0 60 9749.0 61 7662.0 62 6135.0 63 4690.5 64 3437.0 65 2530.5 66 1857.5 67 1570.5 68 1206.5 69 755.5 70 503.5 71 385.5 72 306.5 73 240.5 74 208.0 75 158.5 76 90.0 77 65.0 78 42.0 79 22.0 80 16.0 81 16.0 82 13.0 83 5.5 84 4.5 85 2.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03758158891271446 2 0.0 3 0.0 4 0.0 5 0.01157144290772952 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005171308556109209 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.1201074812962466E-5 22-23 5.632118229425872E-4 24-25 4.096085985036997E-4 26-27 0.002252847291770349 28-29 0.002611254815461086 30-31 3.584075236907373E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01280026870324062 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.0240214962592493E-4 74-75 5.1201074812962466E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.536032244388874E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.008140970895261033 92-93 0.002816059114712936 94-95 0.0 96-97 0.0 98-99 1.536032244388874E-4 100-101 0.013824290199499868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 976542.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.48822016918562 #Duplication Level Percentage of deduplicated Percentage of total 1 79.02853268870219 48.59323817610602 2 12.442282772366589 15.30107645029084 3 3.8679066182376873 7.134920812081473 4 1.611698730821546 3.9640194562860893 5 0.8504478602278807 2.614626263605236 6 0.5014972238336508 1.850170302799133 7 0.3294379078301778 1.4179585426116494 8 0.224700438992338 1.105314405189883 9 0.1678027348066246 0.928610235250308 >10 0.9017252837133104 10.131490706423627 >50 0.050711204587900016 2.1288688334899004 >100 0.02158340416304001 2.259918966712841 >500 8.365658585646479E-4 0.3363078269699076 >1k 5.019395151387887E-4 0.758004303797577 >5k 3.346263434258592E-4 1.4754747183855659 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7894 0.8083625691470515 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6448 0.6602890607879641 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4528 0.46367693350618816 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1523 0.1559584738802837 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1317 0.13486363105734317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.096085985036998E-4 0.0 0.0 0.0 0.0 7 4.096085985036998E-4 0.0 0.0 0.0 0.0 8 4.096085985036998E-4 0.0 0.0 0.0 0.0 9 4.096085985036998E-4 0.0 0.0 0.0 0.0 10-11 4.096085985036998E-4 0.0 0.0 0.0 0.0 12-13 4.6080967331666226E-4 0.0 0.0 1.0240214962592494E-4 0.0 14-15 5.120107481296248E-4 0.0 0.0 1.536032244388874E-4 0.0 16-17 5.120107481296248E-4 0.0 0.0 2.048042992518499E-4 0.0 18-19 5.120107481296248E-4 0.0 0.0 2.048042992518499E-4 0.0 20-21 5.120107481296248E-4 0.0 0.0 3.072064488777748E-4 0.0 22-23 5.632118229425872E-4 0.0 0.0 4.6080967331666226E-4 0.0 24-25 6.144128977555496E-4 0.0 0.0 5.632118229425872E-4 0.0 26-27 6.144128977555496E-4 0.0 0.0 0.001177624720698137 0.0 28-29 6.144128977555496E-4 0.0 0.0 0.0030720644887777485 0.0 30-31 6.144128977555496E-4 0.0 0.0 0.006912145099749934 0.0 32-33 6.144128977555496E-4 0.0 0.0 0.00952339991521102 0.0 34-35 6.144128977555496E-4 0.0 0.0 0.01244186117954988 0.0 36-37 6.144128977555496E-4 0.0 0.0 0.017510767586033166 0.0 38-39 6.144128977555496E-4 0.0 0.0 0.023245287965084963 0.0 40-41 6.144128977555496E-4 0.0 0.0 0.03148866100997192 0.0 42-43 6.144128977555496E-4 0.0 0.0 0.04444253293765143 0.0 44-45 6.144128977555496E-4 0.0 0.0 0.056269981219445764 0.0 46-47 6.144128977555496E-4 0.0 0.0 0.08130730680298441 0.0 48-49 6.144128977555496E-4 0.0 0.0 0.11166954416707114 0.0 50-51 8.192171970073996E-4 0.0 0.0 0.1266202580124562 0.0 52-53 8.192171970073996E-4 0.0 0.0 0.15493445238402442 0.0 54-55 8.192171970073996E-4 0.0 0.0 0.20552111429923137 0.0 56-57 8.192171970073996E-4 0.0 0.0 0.2833467480149343 0.0 58-59 8.192171970073996E-4 0.0 0.0 0.39040819544883887 0.0 60-61 9.72820421446287E-4 0.0 0.0 0.5185644857056839 0.0 62-63 0.0012288257955110993 0.0 0.0 0.6459527598403345 0.0 64-65 0.0012288257955110993 0.0 0.0 0.7815332059450593 0.0 66-67 0.0012288257955110993 0.0 0.0 0.9533128119425484 0.0 68-69 0.0012288257955110993 0.0 0.0 1.1753206723315537 0.0 70-71 0.0013824290199499867 0.0 0.0 1.3374232751893929 0.0 72-73 0.0017408365436407242 0.0 0.0 1.5360834454636871 0.0 74-75 0.0017408365436407242 0.0 0.0 1.750667150004813 0.0 76-77 0.0017920376184536865 0.0 0.0 1.9869601102666348 0.0 78-79 0.001843238693266649 0.0 0.0 2.259708235795286 0.0 80-81 0.001843238693266649 0.0 0.0 2.6201126014037284 0.0 82-83 0.001843238693266649 0.0 0.0 2.98481785729646 0.0 84-85 0.001843238693266649 0.0 0.0 3.454638919780204 0.0 86-87 0.001843238693266649 0.0 0.0 4.003156034251471 0.0 88-89 0.001843238693266649 0.0 0.0 4.731952133139179 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCCG 35 2.7875646E-4 54.286385 5 GGTATCA 3705 0.0 42.58862 1 GTATCAA 6360 0.0 40.20396 1 ATCAACG 8025 0.0 31.78544 3 TATCAAC 8140 0.0 31.511444 2 TCAACGC 8145 0.0 31.317146 4 CAACGCA 8265 0.0 30.862452 5 AACGCAG 8390 0.0 30.401085 6 ACGCAGA 9645 0.0 26.349525 7 CGCAGAG 9690 0.0 26.08009 8 GCAGAGT 10175 0.0 24.370102 9 TACCTGG 740 0.0 23.108393 2 GTACATG 5655 0.0 22.692108 1 GTATAGC 550 0.0 22.467478 1 TACATGG 5725 0.0 22.319054 2 GAGTACT 6205 0.0 22.316084 12-13 AGTACTT 6265 0.0 22.29192 12-13 GTACTTT 6305 0.0 21.73389 14-15 GTCGGGA 110 0.0028768524 21.591177 2 GTACCGT 110 0.0028777074 21.59007 6 >>END_MODULE