FastQCFastQC Report
Thu 26 May 2016
SRR937863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795472
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115160.4119518993572463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92770.33185809051208526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61560.2202132591562355No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38560.137937350114757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85450.037.7383041
GTATCAA131700.035.608681
ATCAACG157700.029.485483
TCAACGC158550.029.360034
TATCAAC159500.029.1522032
CAACGCA160500.029.0038345
AACGCAG165200.028.180146
ACGCAGA186100.024.8886287
CGCAGAG185350.024.7577768
GAGTACT105050.023.42335912-13
GTACATG104650.022.5427631
TACATGG102200.022.2832152
GCAGAGT198800.022.1751469
GTACTTT111050.022.02809114-15
AGAGTAC153350.021.38981410-11
AGTACTT109950.021.3209912-13
ACATGGG104200.020.988973
TAAGGTG11800.020.5511425
GGACCGA6950.019.8216726
AGGACCG7950.019.7376125
Produced by FastQC (version 0.11.5)