##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937863_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2795472 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.489679381514105 31.0 31.0 34.0 30.0 34.0 2 31.630107545344757 33.0 31.0 34.0 30.0 34.0 3 31.648025807448615 33.0 31.0 34.0 30.0 34.0 4 35.26894778413091 37.0 35.0 37.0 33.0 37.0 5 35.252789868759194 37.0 35.0 37.0 33.0 37.0 6 35.314566913923656 37.0 35.0 37.0 33.0 37.0 7 35.24005141171151 37.0 35.0 37.0 32.0 37.0 8 35.22809958389853 37.0 35.0 37.0 32.0 37.0 9 36.81892753710286 39.0 37.0 39.0 33.0 39.0 10-11 36.6827476719495 39.0 37.0 39.0 32.0 39.0 12-13 36.54645780748296 39.0 37.0 39.0 32.0 39.0 14-15 37.63391316385927 40.0 37.0 41.0 32.0 41.0 16-17 37.52451106646748 40.0 37.0 41.0 32.0 41.0 18-19 37.450678990882395 40.0 37.0 41.0 32.0 41.0 20-21 37.329984346114 40.0 37.0 41.0 32.0 41.0 22-23 37.23232230549975 39.0 37.0 41.0 31.5 41.0 24-25 37.266461620792484 40.0 37.0 41.0 31.5 41.0 26-27 37.16724259802996 39.5 37.0 41.0 31.0 41.0 28-29 37.03488874150769 39.0 36.0 41.0 31.0 41.0 30-31 36.77474966660371 39.0 36.0 41.0 30.0 41.0 32-33 36.603826115947506 39.0 36.0 40.5 30.0 41.0 34-35 36.575785055260795 39.0 36.0 41.0 30.0 41.0 36-37 36.41110910787158 39.0 35.0 41.0 30.0 41.0 38-39 36.23335898195367 39.0 35.0 41.0 30.0 41.0 40-41 35.983470591012896 39.0 35.0 40.5 29.0 41.0 42-43 35.68551106932926 39.0 35.0 40.0 27.5 41.0 44-45 35.43908112833897 38.0 35.0 40.0 27.0 41.0 46-47 35.073126470234726 38.0 34.5 40.0 25.5 41.0 48-49 34.7233277600348 38.0 34.0 40.0 24.0 41.0 50-51 33.823488663095176 37.5 33.0 39.5 23.5 40.5 52-53 33.923669240829454 38.0 33.0 39.5 23.0 40.5 54-55 34.1851615755765 38.0 34.0 40.0 23.0 41.0 56-57 33.903908713805755 38.0 33.0 40.0 22.0 41.0 58-59 33.61484625852092 37.5 33.0 40.0 20.5 41.0 60-61 33.24950634454575 37.0 32.5 40.0 19.5 41.0 62-63 32.83827972521277 36.5 32.0 39.5 18.0 41.0 64-65 32.40625643898419 36.0 31.0 39.0 14.0 41.0 66-67 31.928042741977023 35.5 31.0 39.0 10.0 40.0 68-69 31.368075051368784 35.0 30.5 38.5 7.5 40.0 70-71 30.878297296485172 35.0 30.0 38.0 6.5 40.0 72-73 30.388446208726112 34.5 29.5 37.0 2.0 39.0 74-75 29.822030590898425 34.0 29.0 36.5 2.0 39.0 76-77 29.302761573000907 34.0 28.5 36.0 2.0 39.0 78-79 28.70353056657337 34.0 27.5 35.5 2.0 37.5 80-81 28.11255719964285 33.5 26.5 35.0 2.0 37.0 82-83 27.569110511570138 33.0 26.0 35.0 2.0 36.5 84-85 27.00890314766165 33.0 25.5 35.0 2.0 36.0 86-87 26.41952718539123 32.0 24.5 35.0 2.0 35.5 88-89 25.86750949392446 32.0 23.0 34.5 2.0 35.0 90-91 25.22884722150678 31.5 19.5 34.0 2.0 35.0 92-93 24.517825075693835 31.0 17.0 34.0 2.0 35.0 94-95 23.69047695702193 31.0 4.5 34.0 2.0 35.0 96-97 22.76112477606644 30.5 2.0 34.0 2.0 35.0 98-99 21.61392190656891 29.5 2.0 34.0 2.0 35.0 100-101 18.82849980253782 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31735431407368964 1101 2 0.4969187951231113 1101 3 0.3699177635169768 1101 4 0.20226448255301932 1101 5 0.13581300739699032 1101 6 0.27062776871564154 1101 7 0.30905551021618294 1101 8 0.31685208929366837 1101 9 0.40595153430277975 1101 10-11 0.5006036692879974 1101 12-13 0.361669003293855 1101 14-15 0.6798323987458943 1101 16-17 0.6940090425551872 1101 18-19 0.7529682958096942 1101 20-21 0.7045842526032047 1101 22-23 0.6945626895386425 1101 24-25 0.6612755340163545 1101 26-27 0.5692341664438274 1101 28-29 0.5919681871097993 1101 30-31 0.6881452059274622 1101 32-33 0.6090938335171643 1101 34-35 0.6400654765881413 1101 36-37 0.6791151097460002 1101 38-39 0.5679748631909121 1101 40-41 0.6270039749660583 1101 42-43 0.6663749431804149 1101 44-45 0.7864306749621264 1101 46-47 0.9017712460136451 1101 48-49 1.0980461728765363 1101 50-51 0.9273458818881508 1101 52-53 0.809866691141373 1101 54-55 1.0816419549156322 1101 56-57 0.8642398717700885 1101 58-59 0.8956065762548988 1101 60-61 1.0226069862095457 1101 62-63 0.9484613949285645 1101 64-65 0.9967499633644792 1101 66-67 1.0676164235969559 1101 68-69 0.9485793000945755 1101 70-71 1.1247224226776105 1101 72-73 1.153344845960941 1101 74-75 0.9935003921980332 1101 76-77 0.8080108927289835 1101 78-79 1.0848723843964585 1101 80-81 1.1742663093521735 1101 82-83 1.1319524726131256 1101 84-85 1.3212283090015688 1101 86-87 1.3294114400365018 1101 88-89 1.1808044029962694 1101 90-91 1.2451410185711893 1101 92-93 1.2855632786141378 1101 94-95 1.3447026888009184 1101 96-97 1.629808790553927 1101 98-99 1.6066310760924551 1101 100-101 1.620449537328959 1102 1 0.276853772287069 1102 2 0.3534517060045239 1102 3 0.456398992204047 1102 4 0.3822163857113168 1102 5 0.33922599752797566 1102 6 0.2825951446624373 1102 7 0.27847935732612683 1102 8 0.37129527074969104 1102 9 0.42646014977237456 1102 10-11 0.37004922770002935 1102 12-13 0.5153359559034669 1102 14-15 0.5783551006676362 1102 16-17 0.7452364946708059 1102 18-19 0.6413926528364513 1102 20-21 0.5978078697194107 1102 22-23 0.6820815458377112 1102 24-25 0.7644977701078375 1102 26-27 0.5868218482344858 1102 28-29 0.7001057424835793 1102 30-31 0.6547475796964832 1102 32-33 0.7193802766348796 1102 34-35 0.7893341974405459 1102 36-37 0.9340244534405642 1102 38-39 0.9263566209166925 1102 40-41 0.9340474002260635 1102 42-43 0.8174768415582392 1102 44-45 0.8954980317870493 1102 46-47 0.9853808728182685 1102 48-49 1.0516309943525357 1102 50-51 0.9400046997309843 1102 52-53 0.9540836501249359 1102 54-55 0.9211334855159592 1102 56-57 0.9882249513914729 1102 58-59 0.9486218578245982 1102 60-61 0.9509351893901581 1102 62-63 1.02817785993021 1102 64-65 0.9678287826945748 1102 66-67 0.9710085655662972 1102 68-69 0.9959114657538102 1102 70-71 1.0987464100146767 1102 72-73 1.1411965424966937 1102 74-75 1.088580956399749 1102 76-77 0.9716428682317293 1102 78-79 1.2131422260035443 1102 80-81 1.2249368366911497 1102 82-83 1.3507717230354004 1102 84-85 1.3192239640853352 1102 86-87 1.5014117613903295 1102 88-89 1.5388387134931207 1102 90-91 1.612919454401343 1102 92-93 1.6297309058012033 1102 94-95 1.9394664804574084 1102 96-97 2.218425694296503 1102 98-99 2.381584267321884 1102 100-101 2.3634582324730644 1103 1 0.30271724529577426 1103 2 0.31134472181665274 1103 3 0.310625568779205 1103 4 0.2833398045770821 1103 5 0.32360692235984345 1103 6 0.17780685865439239 1103 7 0.28162543322056877 1103 8 0.19430885050362434 1103 9 0.33578047035392444 1103 10-11 0.3178456225724915 1103 12-13 0.3503820339988408 1103 14-15 0.35603072722597773 1103 16-17 0.34006398872043064 1103 18-19 0.23003489753613593 1103 20-21 0.3264791038451875 1103 22-23 0.3124487557154936 1103 24-25 0.294001585581654 1103 26-27 0.33978068765129876 1103 28-29 0.3306814121173076 1103 30-31 0.23028107002967602 1103 32-33 0.3108032424229492 1103 34-35 0.3549763824006291 1103 36-37 0.3092498132144357 1103 38-39 0.22041944810889902 1103 40-41 0.2748970230805483 1103 42-43 0.2296649772740338 1103 44-45 0.1661419932141328 1103 46-47 0.2734162889674536 1103 48-49 0.30612294051143607 1103 50-51 0.2503874419043157 1103 52-53 0.289554822624595 1103 54-55 0.39061504012895654 1103 56-57 0.27989819465425825 1103 58-59 0.2996595975297325 1103 60-61 0.4073532659637422 1103 62-63 0.3674817992850876 1103 64-65 0.4706516359206674 1103 66-67 0.4939315926660619 1103 68-69 0.34450082518810277 1103 70-71 0.46781198837259197 1103 72-73 0.3321591989594168 1103 74-75 0.3203828598920033 1103 76-77 0.497100740810307 1103 78-79 0.6347327456437206 1103 80-81 0.7734629979188945 1103 82-83 0.712269237553695 1103 84-85 0.6922650554963532 1103 86-87 0.5868249235959198 1103 88-89 0.8152359126492215 1103 90-91 0.8400198515124586 1103 92-93 0.7711788678948572 1103 94-95 0.859439629080093 1103 96-97 1.1681928670728539 1103 98-99 1.1109355896107296 1103 100-101 1.0521727935285057 1104 1 0.15514454154650892 1104 2 0.12323607978149198 1104 3 0.13623474105633804 1104 4 0.1009226283731266 1104 5 0.1323440980790025 1104 6 0.12939243364878905 1104 7 0.18331432965067762 1104 8 0.18204117223627492 1104 9 0.22980418883482656 1104 10-11 0.14165409311224408 1104 12-13 0.20680129481122833 1104 14-15 0.2926789313721656 1104 16-17 0.18315993524780083 1104 18-19 0.16167307253417107 1104 20-21 0.26313124693174217 1104 22-23 0.14870700476560472 1104 24-25 0.19091648629004965 1104 26-27 0.1986890557786083 1104 28-29 0.22837228407751553 1104 30-31 0.25214279504316295 1104 32-33 0.3576220935855474 1104 34-35 0.325618853873614 1104 36-37 0.32681082134077144 1104 38-39 0.5160881286850696 1104 40-41 0.5382648776912333 1104 42-43 0.46114317069418576 1104 44-45 0.44957511303670117 1104 46-47 0.5037240111085453 1104 48-49 0.5479499422506322 1104 50-51 0.43341788082346966 1104 52-53 0.5380474204466665 1104 54-55 0.6502181767374609 1104 56-57 0.6252805445654843 1104 58-59 0.5609295119032325 1104 60-61 0.6717346402297366 1104 62-63 0.7801048911714759 1104 64-65 0.9036726819820373 1104 66-67 0.9331773764848954 1104 68-69 0.941701753566047 1104 70-71 0.8373358007820748 1104 72-73 0.8742579875837571 1104 74-75 0.766588448339391 1104 76-77 0.6403360652274976 1104 78-79 0.7582845790689667 1104 80-81 0.8451144441159961 1104 82-83 0.7184579188570623 1104 84-85 0.6398289723786519 1104 86-87 0.784035244831653 1104 88-89 0.8587075734882781 1104 90-91 0.8207248006395318 1104 92-93 0.7290391748898095 1104 94-95 0.6965720198956937 1104 96-97 0.7744388145092103 1104 98-99 0.752846218083814 1104 100-101 0.7741572316841143 1105 1 0.15607234636733835 1105 2 0.2006403375962833 1105 3 0.20739068498924595 1105 4 0.13687359899068952 1105 5 0.15357100559175763 1105 6 0.26088501472907666 1105 7 0.20969987888532415 1105 8 0.2806711646120874 1105 9 0.23262513775278393 1105 10-11 0.29338185101500613 1105 12-13 0.3256353807885901 1105 14-15 0.3726337727304454 1105 16-17 0.4366934523435688 1105 18-19 0.3516935991894954 1105 20-21 0.4118093303286088 1105 22-23 0.5208235961678653 1105 24-25 0.3737157356009462 1105 26-27 0.37407407718497865 1105 28-29 0.33253157092286045 1105 30-31 0.39175454801952725 1105 32-33 0.4634581149332746 1105 34-35 0.48449106078229676 1105 36-37 0.5655827408762804 1105 38-39 0.5674493391712616 1105 40-41 0.6443173086337026 1105 42-43 0.5350144978890654 1105 44-45 0.6059876401612101 1105 46-47 0.7737163869222812 1105 48-49 0.7115573553055796 1105 50-51 0.5533269770641525 1105 52-53 0.6550622582860868 1105 54-55 0.7698551481760916 1105 56-57 0.7341328112947068 1105 58-59 0.7030654570090888 1105 60-61 0.8058124069296397 1105 62-63 0.962112984538436 1105 64-65 0.7627671632441277 1105 66-67 0.8543667495421978 1105 68-69 0.7925058119790442 1105 70-71 0.8625360822904895 1105 72-73 0.8155318131658369 1105 74-75 0.8980898265576762 1105 76-77 0.7725511845753381 1105 78-79 0.7595865554926355 1105 80-81 0.8222465771287233 1105 82-83 0.7791370579104893 1105 84-85 0.664891432058436 1105 86-87 0.8578573080537524 1105 88-89 0.9133002659989771 1105 90-91 0.6978410988038632 1105 92-93 0.7095376793763499 1105 94-95 0.9748120058202758 1105 96-97 1.1472468609580986 1105 98-99 1.2590863213035668 1105 100-101 0.9342147063190325 1106 1 -0.06335166571896522 1106 2 0.09412648083786834 1106 3 -0.027126334981481648 1106 4 0.12584720741332944 1106 5 0.15452870352859094 1106 6 0.029054364618133377 1106 7 0.02682017537746617 1106 8 0.10899537026810435 1106 9 0.08941542149764814 1106 10-11 -0.1269145230938662 1106 12-13 -0.25234500257386117 1106 14-15 -0.08705993861463668 1106 16-17 -0.1015465977012795 1106 18-19 -0.011331163569579417 1106 20-21 -0.1526173964548292 1106 22-23 -0.049699396136553275 1106 24-25 -0.010729521941186704 1106 26-27 0.07206449312378993 1106 28-29 0.08976470439153417 1106 30-31 0.0543863878760007 1106 32-33 0.12325061764796885 1106 34-35 0.12796846978093157 1106 36-37 0.01945944641426678 1106 38-39 0.11881978910280111 1106 40-41 0.2679109774878583 1106 42-43 0.20265566214004593 1106 44-45 0.22127598820898697 1106 46-47 0.2933329064444976 1106 48-49 0.3161106105815392 1106 50-51 0.3945357254707531 1106 52-53 0.3001441713213566 1106 54-55 0.31302154730738607 1106 56-57 0.3169468899501098 1106 58-59 0.21260419455689572 1106 60-61 0.18045371281699119 1106 62-63 0.30553067020011326 1106 64-65 0.4701773421780828 1106 66-67 0.4290504283109051 1106 68-69 0.18060788459128574 1106 70-71 0.002672761389682421 1106 72-73 0.3247359347842682 1106 74-75 0.22918290526028784 1106 76-77 0.28221963743402867 1106 78-79 0.2554460522113935 1106 80-81 0.03607477220922206 1106 82-83 -0.046061941192480305 1106 84-85 0.04404893170434576 1106 86-87 0.14652391963960554 1106 88-89 0.3991969422654442 1106 90-91 0.5086781697636837 1106 92-93 0.5200415507705216 1106 94-95 0.6927097679655922 1106 96-97 0.733636195739269 1106 98-99 0.8900598353019618 1106 100-101 0.9357690474738405 1107 1 0.1388425547548522 1107 2 0.16313488198180437 1107 3 0.1495896651002795 1107 4 0.07907461435686258 1107 5 0.14358988823668284 1107 6 0.03800671167958569 1107 7 0.01877372472605998 1107 8 0.0768794842828342 1107 9 0.06475390156142424 1107 10-11 0.17183234343367815 1107 12-13 0.24082697744624681 1107 14-15 0.2068417758059411 1107 16-17 0.35579601789574866 1107 18-19 0.29850062308113223 1107 20-21 0.4024510010469342 1107 22-23 0.27810630441404527 1107 24-25 0.18166498990607494 1107 26-27 0.27907413371983836 1107 28-29 0.16782719173220784 1107 30-31 0.319934125364874 1107 32-33 0.25795207135619336 1107 34-35 0.2867300675777855 1107 36-37 0.36492919783628963 1107 38-39 0.2981855361839578 1107 40-41 0.40475534076811215 1107 42-43 0.41252967750033065 1107 44-45 0.38705026948471044 1107 46-47 0.5612488662022557 1107 48-49 0.6396962275480931 1107 50-51 0.32785327142989473 1107 52-53 0.28489491508553755 1107 54-55 0.37522562114677527 1107 56-57 0.42004556669096615 1107 58-59 0.37491946137357957 1107 60-61 0.4606782496948725 1107 62-63 0.5307262973684601 1107 64-65 0.5207479640039594 1107 66-67 0.5934894415063248 1107 68-69 0.6884227509553078 1107 70-71 0.6703623167292676 1107 72-73 0.6243794064766313 1107 74-75 0.736759335043562 1107 76-77 0.7657737849529873 1107 78-79 0.9540529229014147 1107 80-81 0.9644845662966439 1107 82-83 0.9416754733512889 1107 84-85 1.056934262872197 1107 86-87 1.1162884655485783 1107 88-89 1.0222456646263147 1107 90-91 1.3341295849132457 1107 92-93 1.510375752215868 1107 94-95 1.575509419915548 1107 96-97 1.830346328399667 1107 98-99 1.7395156470687674 1107 100-101 1.6891356140428258 1108 1 -0.06321688271959403 1108 2 0.020116024270876665 1108 3 0.13228547849366734 1108 4 0.030489624873794696 1108 5 0.09056085413724446 1108 6 0.03797609599144636 1108 7 0.18561990729362066 1108 8 0.1521505939661978 1108 9 0.2766207863241945 1108 10-11 0.2820395029345377 1108 12-13 0.3258046468513953 1108 14-15 0.29732316978702755 1108 16-17 0.3989569279626082 1108 18-19 0.3889404751230572 1108 20-21 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-0.4017692419265977 2316 74-75 -0.5237801171502205 2316 76-77 -0.7639571669580825 2316 78-79 -0.5916761717521268 2316 80-81 -0.40877467852355664 2316 82-83 -0.5886229470112241 2316 84-85 -0.7087561732201024 2316 86-87 -0.6366522123867426 2316 88-89 -0.7964136593755349 2316 90-91 -0.7319918705277075 2316 92-93 -0.9093669316544428 2316 94-95 -0.9573999970097127 2316 96-97 -1.0615460886866153 2316 98-99 -1.3105543935753907 2316 100-101 -1.2405002956804125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12627.0 3 2812.0 4 2576.0 5 3010.0 6 3470.0 7 4227.0 8 5178.0 9 6506.0 10 8039.0 11 10171.0 12 13384.0 13 16290.0 14 18061.0 15 22126.0 16 26119.0 17 31538.0 18 28895.0 19 23214.0 20 23893.0 21 25436.0 22 27982.0 23 30894.0 24 34951.0 25 39431.0 26 44934.0 27 52242.0 28 61517.0 29 73230.0 30 88055.0 31 107511.0 32 131598.0 33 167002.0 34 217642.0 35 290703.0 36 386647.0 37 454870.0 38 276037.0 39 22650.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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22.47447821419821 18-19 21.936539787103857 27.169026862393185 29.27614092854319 21.61829242195977 20-21 22.603249608700033 26.136418620538134 29.677687819504893 21.582643951256944 22-23 22.374953092961416 25.96664340579328 29.603618947468775 22.054784553776525 24-25 22.207544613478884 26.193492102407056 29.67262301592422 21.92634026818984 26-27 21.817875540191693 26.473375930411038 29.621151232170202 22.08759729722707 28-29 21.837645970346465 26.753871139054873 29.548271369478 21.860211521120657 30-31 22.01629932803406 26.382169000446854 29.498514340286246 22.103017331232838 32-33 21.817988883473642 26.377901310670325 29.510184891253672 22.293924914602357 34-35 22.32619599564149 26.507612246057562 29.36045016186677 21.80574159643418 36-37 22.34293368455477 26.229843966293494 29.363832255925743 22.063390093225994 38-39 21.91777238374942 26.696785149941704 29.287543300396397 22.09789916591248 40-41 22.27795601122154 26.517731838466023 29.2480185267214 21.956293623591037 42-43 22.04479679827785 26.49058771895397 29.054101641990133 22.41051384077805 44-45 22.118181353831652 26.643361719249164 29.05742211198024 22.181034814938943 46-47 22.450774437495756 26.967942648305783 28.467440181135515 22.11384273306295 48-49 22.322883015283423 27.0098529009826 28.39314822365503 22.27411586007895 50-51 22.391948216514905 27.233682565372348 28.247629443030007 22.126739775082736 52-53 22.505629298166355 27.139258797122963 28.142887363839108 22.212224540871574 54-55 22.432784262338327 27.170914027940757 28.12217074557194 22.274130964148977 56-57 22.330241820319557 27.42930318579574 28.064752988934412 22.17570200495029 58-59 22.529157585344127 27.024331613466423 28.10531061169113 22.341200189498327 60-61 22.237011202936568 27.234123977444703 28.33836455293333 22.190500266685394 62-63 22.16220409912612 27.25921737179749 28.516408616808324 22.062169912268065 64-65 22.169108332864507 27.245060199258308 28.318484338231 22.267347129646186 66-67 22.129004261369744 27.36842501322272 28.03737262833202 22.465198097075522 68-69 22.25311823116984 27.197921131436615 28.25996895028749 22.288991687106055 70-71 22.362412711427243 27.151075504156292 28.071367126334675 22.415144658081793 72-73 22.273374886138292 27.261377749970794 28.037052803882656 22.428194560008254 74-75 22.156082285956742 27.675737798444988 27.805662427467258 22.362517488131004 76-77 22.371207192181235 27.366165734320997 27.81105400783805 22.451573065659723 78-79 22.38338243879677 27.385885796019217 27.875052907388685 22.355678857795333 80-81 22.402194837716465 27.435435620530445 27.699666875466754 22.462702666286336 82-83 22.485919010750933 27.439761173235404 27.718084225780302 22.356235590233357 84-85 22.410339007951155 27.518390849902712 27.687393175409113 22.383876966737017 86-87 22.48614264028589 27.511017718465602 27.633543311516167 22.369296329732332 88-89 22.556703646416285 27.496052473392158 27.448339942555045 22.498903937636516 90-91 22.470774416590306 27.40333186523029 27.610717845049308 22.515175873130094 92-93 22.551218035198232 27.28565465024823 27.64191897203813 22.521208342515404 94-95 22.541411201508506 27.07640115610396 27.756797468052785 22.625390174334758 96-97 22.53319976801058 27.064518608514987 27.69602147460918 22.706260148865255 98-99 22.399320260800824 27.116587743622787 27.54248190814347 22.941610087432924 100-101 22.86115956702447 26.387872299948732 28.06152700794677 22.68944112508003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4001.0 1 2308.0 2 636.5 3 694.0 4 623.0 5 621.5 6 1122.5 7 2195.0 8 3386.0 9 3848.5 10 3382.0 11 2965.0 12 2993.5 13 3164.5 14 3370.5 15 3458.0 16 3681.0 17 4109.0 18 4386.5 19 4744.0 20 5423.0 21 6321.0 22 7611.5 23 9336.0 24 11346.5 25 13882.0 26 17670.0 27 22013.5 28 24831.5 29 27809.0 30 32404.5 31 37897.0 32 44440.5 33 49415.0 34 53562.0 35 58456.0 36 64743.5 37 70698.0 38 74760.5 39 78246.0 40 81330.0 41 85741.0 42 89245.0 43 92138.0 44 95450.5 45 99260.5 46 102355.5 47 105800.5 48 109441.0 49 108301.5 50 103316.5 51 98240.5 52 95877.0 53 95044.0 54 93855.5 55 87374.5 56 78132.0 57 70572.0 58 64532.5 59 57870.5 60 48247.0 61 39861.0 62 32495.0 63 24607.0 64 18154.0 65 13777.5 66 10195.5 67 7668.5 68 5988.0 69 4653.0 70 3493.0 71 2324.5 72 1648.5 73 1220.5 74 832.0 75 580.0 76 411.0 77 268.0 78 180.0 79 120.0 80 80.0 81 50.0 82 35.0 83 34.0 84 24.0 85 13.5 86 13.0 87 11.0 88 9.5 89 7.5 90 6.5 91 6.0 92 5.5 93 6.5 94 5.0 95 2.0 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3394417829976476 2 0.3001639794639331 3 0.29833960061127424 4 0.301988358316592 5 0.31207610020776455 6 0.2065483038284769 7 0.2064409874253793 8 0.20683448090340378 9 0.20483124137891562 10-11 0.21186046578180714 12-13 0.20039549671754894 14-15 0.2023987362420371 16-17 0.2091060114356359 18-19 0.2025060526451347 20-21 0.19363456332240137 22-23 0.19241831075396212 24-25 0.19703291608715812 26-27 0.19225733614931576 28-29 0.20824748021085526 30-31 0.21357752823136844 32-33 0.2154019070840273 34-35 0.21414988238122218 36-37 0.1863549339789488 38-39 0.17662491343143483 40-41 0.18329641649066775 42-43 0.1957987774515359 44-45 0.20209467309992732 46-47 0.20524262092412301 48-49 0.20334669780273243 50-51 0.20674505056748915 52-53 0.23223269630316454 54-55 0.19409960106915755 56-57 0.17632085028932504 58-59 0.17692897657354464 60-61 0.16922008161770177 62-63 0.17621353388622743 64-65 0.17252900404654384 66-67 0.17242168764344626 68-69 0.18272406234081398 70-71 0.17683954623762999 72-73 0.1719208777623242 74-75 0.179146848904228 76-77 0.18274194840799693 78-79 0.1866947692554245 80-81 0.17614198961749572 82-83 0.17277940898710487 84-85 0.16748513310095753 86-87 0.1654997796436523 88-89 0.17172413102331197 90-91 0.17968343091971586 92-93 0.16837943646010406 94-95 0.1750151673849711 96-97 0.1726899786511902 98-99 0.1785029504856425 100-101 0.15292587441405242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2795472.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.15107865946666 #Duplication Level Percentage of deduplicated Percentage of total 1 74.6103232827512 38.91008838326995 2 13.73257369181655 14.323370615976946 3 4.7923734189583165 7.497823294128969 4 2.242809679735287 4.678597761043527 5 1.2388366229014232 3.2303333083580084 6 0.730098874208982 2.284526629083641 7 0.47211110463772044 1.7234772347778617 8 0.3566659215607387 1.48804100243722 9 0.25779984845703524 1.2100086157753287 >10 1.4099325479842546 13.646482364999521 >50 0.10317479797044071 3.689139155464806 >100 0.04924385731491024 4.54564188862381 >500 0.0028188206751152164 0.9648875957059553 >1k 0.0010312758567028624 0.8413320735919522 >5k 1.3750344756038166E-4 0.5533465967076325 >10k+ 6.875172378019083E-5 0.412903480054759 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11516 0.4119518993572463 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9277 0.33185809051208526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6156 0.2202132591562355 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3856 0.137937350114757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.577213436586022E-5 0.0 5 0.0 0.0 0.0 3.577213436586022E-5 0.0 6 0.0 0.0 0.0 3.577213436586022E-5 0.0 7 0.0 0.0 0.0 3.577213436586022E-5 3.577213436586022E-5 8 0.0 0.0 0.0 3.577213436586022E-5 3.577213436586022E-5 9 0.0 0.0 0.0 3.577213436586022E-5 3.577213436586022E-5 10-11 0.0 0.0 0.0 3.577213436586022E-5 3.577213436586022E-5 12-13 0.0 0.0 0.0 3.577213436586022E-5 1.0731640309758067E-4 14-15 0.0 0.0 0.0 3.577213436586022E-5 1.0731640309758067E-4 16-17 0.0 0.0 0.0 3.577213436586022E-5 1.0731640309758067E-4 18-19 0.0 0.0 0.0 3.577213436586022E-5 1.0731640309758067E-4 20-21 0.0 1.788606718293011E-5 0.0 8.943033591465055E-5 1.4308853746344087E-4 22-23 0.0 3.577213436586022E-5 0.0 1.6097460464637098E-4 1.4308853746344087E-4 24-25 0.0 3.577213436586022E-5 0.0 2.5040494056102156E-4 1.4308853746344087E-4 26-27 0.0 3.577213436586022E-5 0.0 3.2194920929274197E-4 1.4308853746344087E-4 28-29 1.788606718293011E-5 3.577213436586022E-5 0.0 8.943033591465054E-4 1.4308853746344087E-4 30-31 3.577213436586022E-5 3.577213436586022E-5 0.0 0.002414619069695565 1.4308853746344087E-4 32-33 3.577213436586022E-5 3.577213436586022E-5 0.0 0.004775579937842339 1.788606718293011E-4 34-35 3.577213436586022E-5 3.577213436586022E-5 0.0 0.008442223710343012 1.788606718293011E-4 36-37 3.577213436586022E-5 3.577213436586022E-5 0.0 0.01336089218564879 1.788606718293011E-4 38-39 3.577213436586022E-5 3.577213436586022E-5 0.0 0.01953158536375968 1.788606718293011E-4 40-41 3.577213436586022E-5 3.577213436586022E-5 0.0 0.026560809766651212 1.788606718293011E-4 42-43 3.577213436586022E-5 3.577213436586022E-5 0.0 0.0355217294252992 1.788606718293011E-4 44-45 3.577213436586022E-5 3.577213436586022E-5 0.0 0.0463964582725207 1.9674673901223121E-4 46-47 3.577213436586022E-5 3.577213436586022E-5 0.0 0.06004352753309638 2.1463280619516133E-4 48-49 3.577213436586022E-5 3.577213436586022E-5 0.0 0.07478164689183078 2.8617707492688174E-4 50-51 3.577213436586022E-5 3.577213436586022E-5 0.0 0.09282868867940727 3.2194920929274197E-4 52-53 3.577213436586022E-5 3.577213436586022E-5 0.0 0.11645618342805794 3.577213436586022E-4 54-55 3.577213436586022E-5 3.577213436586022E-5 0.0 0.14637957382510003 3.9349347802446243E-4 56-57 3.577213436586022E-5 3.577213436586022E-5 0.0 0.1771436093797398 3.9349347802446243E-4 58-59 3.577213436586022E-5 3.577213436586022E-5 0.0 0.21397102170939292 4.1137954520739254E-4 60-61 3.577213436586022E-5 3.577213436586022E-5 0.0 0.2605999988552917 4.2926561239032266E-4 62-63 3.577213436586022E-5 3.577213436586022E-5 0.0 0.3028468895413726 4.6503774675618284E-4 64-65 3.577213436586022E-5 3.577213436586022E-5 0.0 0.34675718447546605 4.6503774675618284E-4 66-67 3.577213436586022E-5 3.577213436586022E-5 0.0 0.4089291540033311 4.6503774675618284E-4 68-69 3.577213436586022E-5 3.577213436586022E-5 0.0 0.47997261285392945 4.6503774675618284E-4 70-71 3.577213436586022E-5 3.577213436586022E-5 0.0 0.546383580304149 4.6503774675618284E-4 72-73 3.577213436586022E-5 5.3658201548790326E-5 0.0 0.6266204776867734 4.8292381393911295E-4 74-75 3.577213436586022E-5 7.154426873172043E-5 0.0 0.7168020284231071 5.365820154879032E-4 76-77 3.577213436586022E-5 7.154426873172043E-5 0.0 0.8163916504976619 5.365820154879032E-4 78-79 3.577213436586022E-5 7.154426873172043E-5 0.0 0.9323470240445979 5.365820154879032E-4 80-81 3.577213436586022E-5 7.154426873172043E-5 0.0 1.066385211513476 5.365820154879032E-4 82-83 3.577213436586022E-5 7.154426873172043E-5 0.0 1.2070770159744044 5.365820154879032E-4 84-85 7.154426873172043E-5 8.943033591465055E-5 0.0 1.3506305911846015 5.365820154879032E-4 86-87 7.154426873172043E-5 1.0731640309758067E-4 0.0 1.5136263214226435 5.544680826708333E-4 88-89 7.154426873172043E-5 1.0731640309758067E-4 0.0 1.6940430810968596 5.723541498537635E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8545 0.0 37.738304 1 GTATCAA 13170 0.0 35.60868 1 ATCAACG 15770 0.0 29.48548 3 TCAACGC 15855 0.0 29.36003 4 TATCAAC 15950 0.0 29.152203 2 CAACGCA 16050 0.0 29.003834 5 AACGCAG 16520 0.0 28.18014 6 ACGCAGA 18610 0.0 24.888628 7 CGCAGAG 18535 0.0 24.757776 8 GAGTACT 10505 0.0 23.423359 12-13 GTACATG 10465 0.0 22.542763 1 TACATGG 10220 0.0 22.283215 2 GCAGAGT 19880 0.0 22.175146 9 GTACTTT 11105 0.0 22.028091 14-15 AGAGTAC 15335 0.0 21.389814 10-11 AGTACTT 10995 0.0 21.32099 12-13 ACATGGG 10420 0.0 20.98897 3 TAAGGTG 1180 0.0 20.551142 5 GGACCGA 695 0.0 19.821672 6 AGGACCG 795 0.0 19.737612 5 >>END_MODULE