FastQCFastQC Report
Thu 26 May 2016
SRR937863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795472
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126180.45137279142842424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113820.407158433352221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77140.27594624449824573No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28810.1030595191080433No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28120.10059124183679895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97150.037.6176951
GTATCAA140100.030.529031
ATCAACG175950.024.1972063
TATCAAC176600.024.1350522
TCAACGC177900.023.9319744
CAACGCA180250.023.6199635
AACGCAG183400.023.230216
GTACATG103800.021.2436561
GAGTACT125500.021.1765512-13
TACATGG100850.020.9667242
AGAGTAC177700.020.66288210-11
ACGCAGA208600.020.3118467
CGCAGAG208900.020.2599378
GTACTTT129850.020.24583414-15
AGTACTT130300.020.08658212-13
TAACGCC1908.233112E-620.0070424
ACATGGG104350.019.489373
GCAGAGT223500.018.4689039
CCTATAC7100.018.0697383
TACTTTT146300.017.74213414-15
Produced by FastQC (version 0.11.5)