##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937863_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2795472 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66846099692646 31.0 31.0 34.0 30.0 34.0 2 31.968466863556493 31.0 31.0 34.0 30.0 34.0 3 32.06555350938947 33.0 31.0 34.0 30.0 34.0 4 35.81489887933058 37.0 35.0 37.0 35.0 37.0 5 35.68608127715105 37.0 35.0 37.0 33.0 37.0 6 35.72185841961572 37.0 35.0 37.0 33.0 37.0 7 35.64624614376392 37.0 35.0 37.0 33.0 37.0 8 35.61596932467934 37.0 35.0 37.0 33.0 37.0 9 37.2297061104529 39.0 37.0 39.0 33.0 39.0 10-11 37.11950504244006 39.0 37.0 39.0 33.0 39.0 12-13 36.98311340625125 39.0 37.0 39.0 33.0 39.0 14-15 38.06016908772472 40.0 37.0 41.0 33.0 41.0 16-17 37.99571235197491 40.0 37.0 41.0 33.0 41.0 18-19 37.961326387815724 40.0 37.0 41.0 33.0 41.0 20-21 37.828200926355194 40.0 37.0 41.0 32.5 41.0 22-23 37.76205216864987 39.0 37.0 41.0 32.5 41.0 24-25 37.78943645294963 39.5 37.0 41.0 32.0 41.0 26-27 37.60257230263798 39.0 37.0 41.0 32.0 41.0 28-29 37.421713220522335 39.0 36.0 41.0 32.0 41.0 30-31 37.46176996228186 39.0 36.5 41.0 32.0 41.0 32-33 37.3672209916608 39.0 36.0 41.0 32.0 41.0 34-35 37.19221995426891 39.0 36.0 41.0 31.0 41.0 36-37 37.00530196689503 39.0 36.0 41.0 31.0 41.0 38-39 36.84716123073313 39.0 35.0 40.5 31.0 41.0 40-41 36.63745406857947 39.0 35.0 40.0 30.0 41.0 42-43 36.387165924037156 39.0 35.0 40.0 30.0 41.0 44-45 36.06892449647144 38.5 35.0 40.0 29.5 41.0 46-47 35.69220314136575 38.0 35.0 40.0 28.5 41.0 48-49 35.33354081171265 38.0 34.5 40.0 27.0 41.0 50-51 35.026341347722315 38.0 34.0 40.0 26.0 41.0 52-53 34.7374781432259 38.0 34.0 40.0 26.0 41.0 54-55 34.46163867854874 38.0 33.0 40.0 25.0 41.0 56-57 34.11970661841721 37.5 33.0 40.0 24.0 41.0 58-59 33.795558496024995 37.0 33.0 40.0 23.0 41.0 60-61 33.39699270820813 36.5 32.0 39.0 22.5 40.5 62-63 32.94343441823062 36.0 31.5 39.0 22.0 40.0 64-65 32.45756494788716 35.5 31.0 39.0 20.5 40.0 66-67 31.964081736465253 35.0 31.0 38.0 18.5 40.0 68-69 31.441884769369896 35.0 30.0 38.0 18.0 40.0 70-71 30.993500560907066 34.0 29.5 37.0 15.5 39.0 72-73 30.436772752508343 34.0 29.0 36.5 12.0 39.0 74-75 29.897829060709604 34.0 29.0 36.0 8.0 39.0 76-77 28.787111979658533 32.5 27.0 35.0 7.5 37.0 78-79 29.400238135098473 33.5 29.0 35.0 7.0 37.0 80-81 29.188873471098976 34.0 29.0 35.0 4.5 37.0 82-83 28.744250344843373 33.0 28.0 35.0 2.0 36.5 84-85 28.25520269922217 33.0 27.0 35.0 2.0 36.0 86-87 27.749944910913076 33.0 26.0 35.0 2.0 35.5 88-89 27.152240659180272 32.0 25.5 34.5 2.0 35.0 90-91 26.41726853282737 32.0 25.0 34.0 2.0 35.0 92-93 25.77904822512978 31.5 23.5 34.0 2.0 35.0 94-95 25.083513267169195 31.0 20.0 34.0 2.0 35.0 96-97 24.23174887818587 31.0 14.0 34.0 2.0 35.0 98-99 22.99325874127875 30.5 2.0 34.0 2.0 35.0 100-101 20.08785886605196 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2442666889890326 1101 2 0.20152487144980924 1101 3 0.2428008876036074 1101 4 0.056274592576471605 1101 5 0.07334665586656541 1101 6 0.18527446994521313 1101 7 0.19612222927649015 1101 8 0.078746760756637 1101 9 0.10310820543956112 1101 10-11 0.20285271241590408 1101 12-13 0.24937200540124138 1101 14-15 0.2979969797894739 1101 16-17 0.3131668674592021 1101 18-19 0.29454740490776743 1101 20-21 0.33983624851933314 1101 22-23 0.27626026135376236 1101 24-25 0.21842012159250146 1101 26-27 0.03879961399544385 1101 28-29 -0.02238999777538453 1101 30-31 0.07665887587103981 1101 32-33 0.27496516134093696 1101 34-35 0.19765649071170088 1101 36-37 0.09733343737841693 1101 38-39 0.12366388547401641 1101 40-41 0.26873661721171516 1101 42-43 0.22172948017726668 1101 44-45 0.17214711989972642 1101 46-47 0.02045488674394136 1101 48-49 0.10201252991703313 1101 50-51 0.09847309541518001 1101 52-53 0.1459683441470645 1101 54-55 0.22799987997554183 1101 56-57 0.2589158774838012 1101 58-59 0.2500452539050073 1101 60-61 0.40796414977202033 1101 62-63 0.057303846487762655 1101 64-65 0.1219738285330294 1101 66-67 0.06591086903912924 1101 68-69 0.03694451619537631 1101 70-71 0.06917670030928846 1101 72-73 0.043433486723571946 1101 74-75 -0.10372244355205851 1101 76-77 -0.06611451879006935 1101 78-79 -0.05041436710764202 1101 80-81 0.03799223312577382 1101 82-83 -0.08671477478239709 1101 84-85 0.11106165703347415 1101 86-87 -0.01709075829968043 1101 88-89 -0.10517261871313721 1101 90-91 -0.19513243321758722 1101 92-93 -0.3433042205790393 1101 94-95 -0.5467892392807805 1101 96-97 -0.6639816095322644 1101 98-99 -0.6034745278412466 1101 100-101 -0.5244299429635468 1102 1 0.3741512671922145 1102 2 0.311844047509215 1102 3 0.2644436696247894 1102 4 0.1666240449470564 1102 5 0.18446324091113553 1102 6 0.19168986093455231 1102 7 0.20517444794581507 1102 8 0.18082307830734123 1102 9 0.24817051090097664 1102 10-11 0.2441642742486323 1102 12-13 0.3282053449314759 1102 14-15 0.473787151673946 1102 16-17 0.38907170423270543 1102 18-19 0.40384840721301174 1102 20-21 0.4327837270868855 1102 22-23 0.3483783681813364 1102 24-25 0.2387214505632116 1102 26-27 0.2327312718625052 1102 28-29 0.2683642409081841 1102 30-31 0.4688785278528087 1102 32-33 0.42389851893072716 1102 34-35 0.25092387568827235 1102 36-37 0.19707974048147037 1102 38-39 0.3850167718156783 1102 40-41 0.48976949091169786 1102 42-43 0.4792358143002602 1102 44-45 0.45889067688824525 1102 46-47 0.3723587089954279 1102 48-49 0.2509007386217448 1102 50-51 0.21984027005105844 1102 52-53 0.30881140927832007 1102 54-55 0.2635418126665243 1102 56-57 0.36196282966847804 1102 58-59 0.10257193158445688 1102 60-61 0.21106169526616014 1102 62-63 0.15705200866256064 1102 64-65 0.14983991959567788 1102 66-67 0.23732664512191093 1102 68-69 0.2746520018805221 1102 70-71 0.14907847492605697 1102 72-73 0.11336758263042412 1102 74-75 0.15658418377310568 1102 76-77 -0.03198528143441237 1102 78-79 0.1912285742625457 1102 80-81 0.3445043622409649 1102 82-83 0.2593204227572521 1102 84-85 0.20378299280307743 1102 86-87 0.16420605047089154 1102 88-89 0.10515425921490262 1102 90-91 0.15195958021022093 1102 92-93 0.16988376875519862 1102 94-95 0.04713352160010231 1102 96-97 0.038647649069925905 1102 98-99 0.12209474648337348 1102 100-101 -0.02426986360774208 1103 1 0.3782097868449661 1103 2 0.20895422304930733 1103 3 0.2469242463948902 1103 4 0.18696066186216598 1103 5 0.13889513305286272 1103 6 0.12906766987474327 1103 7 0.19914389271341548 1103 8 0.09315364580208296 1103 9 0.12255762978592344 1103 10-11 0.16101775816767372 1103 12-13 0.2835125793968274 1103 14-15 0.2462896434440296 1103 16-17 0.27200944613877454 1103 18-19 0.22025667391572767 1103 20-21 0.1189725608127219 1103 22-23 0.13792676826056294 1103 24-25 0.17428883525793282 1103 26-27 0.07962056809859774 1103 28-29 0.16541279033641132 1103 30-31 0.23719201611083207 1103 32-33 0.22448491025024708 1103 34-35 0.33418672677632344 1103 36-37 0.3088437250497904 1103 38-39 0.23052010937271206 1103 40-41 0.2531220771646687 1103 42-43 0.2855612944965813 1103 44-45 0.36602704352588233 1103 46-47 0.2493985804609693 1103 48-49 0.11541636052668025 1103 50-51 0.11114155571009832 1103 52-53 0.06399696361462759 1103 54-55 -0.09783685149317023 1103 56-57 -0.0389096828847002 1103 58-59 -0.0840218737336258 1103 60-61 -0.053627485892597804 1103 62-63 -0.023426977147714467 1103 64-65 -0.0757833373443404 1103 66-67 0.10499903316518555 1103 68-69 0.0737732009485832 1103 70-71 0.016702128033930563 1103 72-73 -0.11479775142397486 1103 74-75 -0.23539939098156637 1103 76-77 -0.17034344862353734 1103 78-79 -0.10305403176737826 1103 80-81 -0.002735196692146502 1103 82-83 -0.15427693863901837 1103 84-85 -0.14444604252818039 1103 86-87 -0.19369168162531025 1103 88-89 -0.07415571698358647 1103 90-91 0.1203670797307339 1103 92-93 0.03809947272348069 1103 94-95 -0.17388569882958294 1103 96-97 -0.4501907930471205 1103 98-99 -0.4050062794656206 1103 100-101 -0.44125414580502564 1104 1 0.2666705095496269 1104 2 0.20418810520832764 1104 3 0.17654507793550067 1104 4 0.05835122900897716 1104 5 0.09167935789969306 1104 6 0.0871294688191071 1104 7 0.03776065805558915 1104 8 0.08188039847904349 1104 9 0.04300194225903198 1104 10-11 0.05270235206292284 1104 12-13 0.11647635932519051 1104 14-15 0.021881241987223632 1104 16-17 0.06707546883919235 1104 18-19 0.15134612422145466 1104 20-21 0.05643551962351978 1104 22-23 0.1649842586101613 1104 24-25 0.13666862182795825 1104 26-27 0.05740373246148778 1104 28-29 0.017537394169906406 1104 30-31 0.02330846330782066 1104 32-33 0.19630724586009052 1104 34-35 0.1487865607348411 1104 36-37 0.17567118128210524 1104 38-39 3.690358599612864E-4 1104 40-41 -0.05726945350755841 1104 42-43 0.1384361149718032 1104 44-45 0.04584967001131446 1104 46-47 -0.08519379981039776 1104 48-49 -0.1142244687111642 1104 50-51 -0.09045462412373695 1104 52-53 -0.004568474304647907 1104 54-55 -0.23234196908569515 1104 56-57 -0.10810171531733204 1104 58-59 -0.1484965705867154 1104 60-61 -0.014381702073769986 1104 62-63 -0.22024946425526082 1104 64-65 -0.20871772671767985 1104 66-67 -0.09215190096967518 1104 68-69 -0.1566675361786558 1104 70-71 -0.12571450277827978 1104 72-73 -0.30060059836887376 1104 74-75 -0.3292876705130183 1104 76-77 -0.25534820565151506 1104 78-79 -0.2018562909011763 1104 80-81 -0.15092934403275748 1104 82-83 -0.3001019648481211 1104 84-85 -0.16811695315059438 1104 86-87 -0.28767873766128105 1104 88-89 -0.2700689148577702 1104 90-91 -0.20411131366639168 1104 92-93 -0.2366443446551223 1104 94-95 -0.1733733335867953 1104 96-97 -0.19611035382497022 1104 98-99 -0.047130216709209805 1104 100-101 -0.1746972644762117 1105 1 0.2342571613589044 1105 2 0.19991269614141416 1105 3 0.1553252081331422 1105 4 0.10250816995085898 1105 5 0.010003209873190144 1105 6 0.07135209505803886 1105 7 0.16362072491383373 1105 8 0.004775243356178294 1105 9 0.12888194929674057 1105 10-11 0.22428173157145181 1105 12-13 0.24411989929352274 1105 14-15 0.17196686746373757 1105 16-17 0.24361115589566396 1105 18-19 0.26370082482552704 1105 20-21 -0.10165256905162323 1105 22-23 -0.005791062485371867 1105 24-25 0.1355854009025066 1105 26-27 0.13996311314297571 1105 28-29 0.02934315336291604 1105 30-31 0.1307103752191452 1105 32-33 0.1768221716708922 1105 34-35 0.21102337968973472 1105 36-37 0.08433988106510526 1105 38-39 0.18731936135406357 1105 40-41 0.30435215226336254 1105 42-43 0.1362080931548988 1105 44-45 0.15048517526027183 1105 46-47 0.2116761784900021 1105 48-49 0.1817564121755808 1105 50-51 0.27769621746912776 1105 52-53 0.3351142422515636 1105 54-55 0.09985438303441185 1105 56-57 0.1168610434651427 1105 58-59 0.20523693477954197 1105 60-61 0.15680767098353243 1105 62-63 0.04647506803023305 1105 64-65 0.10037030330988017 1105 66-67 0.10259914002499215 1105 68-69 0.17094022876181825 1105 70-71 -0.12402897113790701 1105 72-73 -0.030968905213047293 1105 74-75 -0.056726530403928166 1105 76-77 -0.07843600108873261 1105 78-79 0.22603884721389278 1105 80-81 0.14167930062151513 1105 82-83 0.16609825801270617 1105 84-85 0.4216425917441313 1105 86-87 0.2541616236078532 1105 88-89 0.35017388615096934 1105 90-91 0.4919598845038031 1105 92-93 0.48685574331625503 1105 94-95 0.3395590457457942 1105 96-97 0.3353083312961367 1105 98-99 0.32978667966050423 1105 100-101 0.013135120721138094 1106 1 0.15757782610561222 1106 2 0.1212785428908596 1106 3 0.04878727319145071 1106 4 0.002125987806486762 1106 5 0.03763457760842215 1106 6 -0.08722186273432442 1106 7 -0.010563213521557202 1106 8 -0.21532083331631213 1106 9 -0.03753559472855983 1106 10-11 -0.17485605701265428 1106 12-13 -0.3802209014240958 1106 14-15 -0.1512997394009119 1106 16-17 -0.11439531216532828 1106 18-19 0.023096155546447505 1106 20-21 0.21040765001262685 1106 22-23 0.14951982935165375 1106 24-25 0.1120894611451746 1106 26-27 0.154386653108034 1106 28-29 0.11748582840186828 1106 30-31 0.08573858446327876 1106 32-33 0.12866946405556945 1106 34-35 -0.1883233225433969 1106 36-37 0.07371373026958139 1106 38-39 0.07338399475192148 1106 40-41 -0.03024477422735572 1106 42-43 -0.2577958490574659 1106 44-45 -0.046560644106136806 1106 46-47 -0.040305196327054205 1106 48-49 -0.021464398928706885 1106 50-51 0.15309917294809594 1106 52-53 0.2040773459766001 1106 54-55 0.0615668848048756 1106 56-57 -0.0438579971844959 1106 58-59 -3.086013141242461E-4 1106 60-61 0.010739260615743262 1106 62-63 -0.1476349834309758 1106 64-65 -0.4888858696753431 1106 66-67 -0.5279648087970656 1106 68-69 -0.14027926040300187 1106 70-71 -0.2913801349167642 1106 72-73 -0.2904940692751765 1106 74-75 -0.18068621124997009 1106 76-77 -0.22467874222465767 1106 78-79 -0.25247857405989294 1106 80-81 -0.5818863950491355 1106 82-83 -0.6606128273940222 1106 84-85 -0.7378200162536004 1106 86-87 -0.45050210803843527 1106 88-89 -0.4681590537479998 1106 90-91 -0.6490879885900043 1106 92-93 -0.589463835551598 1106 94-95 -0.35018223181428 1106 96-97 -0.3631387439127778 1106 98-99 -0.2878758480766166 1106 100-101 -0.443179736934308 1107 1 0.13487768803770805 1107 2 0.10768963217786975 1107 3 0.10867399432959246 1107 4 0.012278299189560471 1107 5 -0.012290070392751318 1107 6 -0.04781978223869032 1107 7 -0.018371111451649824 1107 8 0.05498634454227869 1107 9 0.08930709148192761 1107 10-11 0.10800138648264124 1107 12-13 0.14419056787484408 1107 14-15 0.07683610232086835 1107 16-17 -2.679273349599498E-4 1107 18-19 0.052114456512185825 1107 20-21 0.08670644995539334 1107 22-23 0.03389121157303521 1107 24-25 0.010267053077399169 1107 26-27 0.03481877963249502 1107 28-29 -0.019257608727059505 1107 30-31 -0.026323240411613824 1107 32-33 0.017532814924585693 1107 34-35 0.03323536281973105 1107 36-37 0.040724815654265 1107 38-39 0.07585476914005085 1107 40-41 0.028724784865893582 1107 42-43 0.013309098344798542 1107 44-45 0.017969260695757328 1107 46-47 0.012030878233332487 1107 48-49 0.021718986094917625 1107 50-51 0.21310807396103115 1107 52-53 0.18442926582660846 1107 54-55 0.10767225031317196 1107 56-57 0.07400377138545622 1107 58-59 0.03510079882449446 1107 60-61 0.12841868849803006 1107 62-63 -0.01887946210850089 1107 64-65 0.26204859731379315 1107 66-67 0.058257714667973914 1107 68-69 0.16608003948308792 1107 70-71 0.203254867805569 1107 72-73 0.13813999224401385 1107 74-75 -0.1343911881983928 1107 76-77 -0.005180158210432495 1107 78-79 0.087777859662868 1107 80-81 0.036612985224810046 1107 82-83 -0.028980155845118816 1107 84-85 0.1242857097550143 1107 86-87 0.02995712569398634 1107 88-89 -0.018404714271913036 1107 90-91 -0.03938881123685789 1107 92-93 -0.09128561646031841 1107 94-95 0.10273438297626569 1107 96-97 0.11250646338419656 1107 98-99 -0.0037583955874005426 1107 100-101 0.0439451522675931 1108 1 0.10556050440591846 1108 2 0.03234098493648574 1108 3 -0.008528849912210035 1108 4 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64-65 -0.48415213662982737 2316 66-67 -0.4125366685203389 2316 68-69 -0.3519475869199944 2316 70-71 -0.35899498055068335 2316 72-73 -0.31568421481961906 2316 74-75 -0.09965956433299539 2316 76-77 -0.2071194425972358 2316 78-79 -0.34628604743067015 2316 80-81 -0.5130218590573428 2316 82-83 -0.41631030576963113 2316 84-85 -0.5655412650339855 2316 86-87 -0.5162505699385527 2316 88-89 -0.41539072672546595 2316 90-91 -0.1005895633414049 2316 92-93 -0.029922687272193826 2316 94-95 -0.0845216703635856 2316 96-97 0.1258346470856111 2316 98-99 -0.006081541447496619 2316 100-101 0.3306158183740955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 24.0 5 64.0 6 185.0 7 513.0 8 1168.0 9 2137.0 10 3489.0 11 5405.0 12 7737.0 13 10493.0 14 13979.0 15 18742.0 16 24042.0 17 30733.0 18 28448.0 19 21293.0 20 20445.0 21 21962.0 22 23288.0 23 26403.0 24 30983.0 25 37132.0 26 44770.0 27 54628.0 28 66407.0 29 80627.0 30 97452.0 31 118462.0 32 145187.0 33 182354.0 34 236361.0 35 315293.0 36 421433.0 37 474668.0 38 220186.0 39 8964.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.46595749705009 20.235376954468077 15.832559930103907 22.46610561837792 2 15.996583045725373 21.085634197015747 39.03026036390277 23.887522393356114 3 19.76861152606787 24.143006976997086 30.254676133404306 25.83370536353074 4 13.558073040285517 17.389913900018993 36.26662555254553 32.78538750714996 5 13.625700921034126 37.50387834920281 34.21990517324367 14.650515556519403 6 32.209480187961105 36.680281541006316 16.543324347373183 14.566913923659403 7 28.34866527012254 33.059604961165775 20.151874173663696 18.43985559504799 8 26.39396853196884 35.64027827858766 19.074560575101447 18.89119261434205 9 25.857064567271642 16.953594956415234 20.26580842161896 36.923532054694164 10-11 25.122054522456317 27.058811535225537 26.573580418619823 21.245553523698323 12-13 25.787165631350362 24.139829499690826 27.652076484562016 22.42092838439679 14-15 22.560465638718615 25.61542379963026 26.098776879181763 25.725333682469365 16-17 21.803616705872926 28.952105404740237 26.90416859836192 22.340109291024916 18-19 21.807086602906413 27.22415034026454 29.522975011017817 21.445788045811227 20-21 22.412243799973673 26.171752033288115 29.988477795520758 21.427526371217454 22-23 22.192499356544477 26.046095919678148 29.879534751695925 21.881869972081454 24-25 22.151214421201463 26.268379081495315 29.94552789470813 21.63487860259509 26-27 21.776406974142358 26.491799771806985 29.87659726615537 21.85519598789529 28-29 21.784229470688356 26.80209426630923 29.819774475735862 21.593901787266557 30-31 21.881260123514025 26.41081720725516 29.789244893170096 21.918677776060715 32-33 21.774873795909958 26.402339211410453 29.743188270174052 22.079598722505537 34-35 22.28696978542443 26.428274008825703 29.651343315189706 21.633412890560162 36-37 22.335906065236927 26.20779245866172 29.64733683614073 21.80896463996062 38-39 21.877343074800965 26.71321694511696 29.569532443894985 21.83990753618709 40-41 22.23164228331549 26.511557610082804 29.5106785037485 21.746121602853204 42-43 21.999247987418276 26.51012498005485 29.307203937082804 22.183423095444063 44-45 22.097842511032127 26.64271006828185 29.325548601452635 21.933898819233388 46-47 22.466241836798936 26.962119456034618 28.700913477223168 21.87072522994328 48-49 22.366330265515092 27.012164672012457 28.549293285713468 22.072211776758987 50-51 22.406663347012596 27.208553689681025 28.419959133913704 21.964823829392675 52-53 22.618827875936514 27.194065975262856 28.1546550993893 22.032451049411332 54-55 22.5100175587867 27.200343842432023 28.19994444576603 22.089694153015248 56-57 22.457674410618313 27.3928874980683 28.125268291007743 22.024169800305636 58-59 22.594842659844204 27.069686264072757 28.17803218919739 22.15743888688565 60-61 22.33808816543324 27.207480525650052 28.437827315029445 22.01660399388726 62-63 22.24375704711047 27.246668898847854 28.560132242426324 21.949441811615355 64-65 22.2406846215388 27.235781155343563 28.36535804425121 22.158176178866427 66-67 22.161016100322236 27.32286354504713 28.17742406291317 22.338696291717465 68-69 22.31522977157346 27.18598147289617 28.314091502257938 22.184697253272432 70-71 22.398095920831974 27.19098957170739 28.10155136592318 22.309363141537457 72-73 22.399151198795767 27.260369626309977 27.972986315012278 22.36749285988198 74-75 22.208074343080526 27.72710654944854 27.74746089390271 22.317358213568227 76-77 22.352450677381135 27.391975308641975 27.94057676127681 22.31499725270008 78-79 22.393159366289485 27.420932851411138 27.919596404471232 22.266311377828146 80-81 22.414837279715197 27.387986715660183 27.8404147850524 22.35676121957222 82-83 22.47056201624567 27.47002006472654 27.81066252947197 22.248755389555825 84-85 22.34742469250273 27.556455582456202 27.76667053005718 22.329449194983887 86-87 22.439609482763558 27.541967868038025 27.729074016838663 22.289348632359758 88-89 22.4503590091405 27.521237916173014 27.61780479289365 22.410598281792844 90-91 22.384142968867952 27.43828006322434 27.695155781886037 22.482421186021668 92-93 22.43810215413894 27.353012458716137 27.723527575786573 22.48535781135835 94-95 22.381408220150302 27.23345109519967 27.80555484011287 22.579585844537164 96-97 22.361304280636688 27.171332783873346 27.79677278112605 22.670590154363914 98-99 22.21329695975869 27.254907642314112 27.564358475744033 22.96743692218316 100-101 22.362697400104047 26.938544335907732 28.01983070944179 22.678927554546437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 882.0 1 611.0 2 467.5 3 624.5 4 522.5 5 487.0 6 1022.0 7 2311.5 8 3629.0 9 4181.5 10 3737.5 11 3060.5 12 3016.0 13 3264.0 14 3564.0 15 3784.0 16 3944.5 17 4264.0 18 4645.5 19 5028.0 20 5683.0 21 6702.5 22 8063.0 23 9839.5 24 11812.0 25 14227.5 26 17995.5 27 22424.5 28 25339.5 29 28334.5 30 33111.0 31 38670.5 32 45036.0 33 49789.5 34 53805.0 35 58720.0 36 65295.0 37 71617.5 38 75346.0 39 78881.0 40 82530.5 41 86500.5 42 89305.5 43 92342.5 44 96107.5 45 99320.0 46 102246.5 47 106833.5 48 110399.5 49 108523.0 50 103310.5 51 98042.0 52 95523.5 53 95107.5 54 94681.5 55 87546.0 56 77662.5 57 69743.5 58 63225.5 59 56544.5 60 46814.5 61 38577.5 62 31338.5 63 23478.0 64 17081.0 65 12723.0 66 9374.5 67 7356.5 68 5753.5 69 4308.0 70 3253.5 71 2132.5 72 1400.0 73 1001.5 74 707.5 75 497.5 76 343.5 77 199.0 78 116.0 79 84.0 80 50.0 81 27.0 82 16.0 83 12.5 84 10.5 85 7.0 86 4.0 87 3.0 88 2.0 89 1.5 90 2.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016669814614490863 2 0.0 3 0.0 4 3.5772134365860224E-5 5 0.03981438554920242 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0039528208474275545 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.617844857684141E-4 24-25 1.6097460464637098E-4 26-27 0.001126822232524597 28-29 0.0010731640309758065 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.975566201342743E-4 42-43 0.010946273115953227 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.9674673901223121E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.7886067182930112E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.796705529513442E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012377158490587637 92-93 0.0014129993074514786 94-95 0.0 96-97 0.0 98-99 0.0012877968371709679 100-101 0.018386877064052155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2795472.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.64845280622529 #Duplication Level Percentage of deduplicated Percentage of total 1 73.3986966973033 37.1753042571181 2 14.26408651681365 14.449078255415015 3 5.03003796668691 7.642909217077902 4 2.368393788055739 4.79821924003593 5 1.3012009109747336 3.2951906465460574 6 0.7618073076933435 2.3150616882686315 7 0.5183355281494578 1.8377024774687372 8 0.38621497596775906 1.5648952786688386 9 0.27226334081577824 1.241074527135586 >10 1.5336794353012198 14.297939825134105 >50 0.10967889629491624 3.8027123161659313 >100 0.05143291113841871 4.649909263082754 >500 0.0030404096035221463 1.0595368753315468 >1k 9.19193601044996E-4 0.7347211023438062 >5k 7.07072000803843E-5 0.27625456148762534 >10k+ 1.414144001607686E-4 0.8594904687196018 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12618 0.45137279142842424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11382 0.407158433352221 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7714 0.27594624449824573 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2881 0.1030595191080433 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2812 0.10059124183679895 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.577213436586022E-5 6 0.0 0.0 0.0 0.0 3.577213436586022E-5 7 0.0 0.0 0.0 0.0 3.577213436586022E-5 8 0.0 0.0 0.0 0.0 3.577213436586022E-5 9 0.0 0.0 0.0 0.0 3.577213436586022E-5 10-11 0.0 0.0 0.0 0.0 3.577213436586022E-5 12-13 0.0 0.0 0.0 0.0 1.4308853746344087E-4 14-15 0.0 0.0 0.0 0.0 1.9674673901223121E-4 16-17 0.0 0.0 0.0 0.0 2.1463280619516133E-4 18-19 0.0 0.0 0.0 0.0 2.1463280619516133E-4 20-21 0.0 0.0 0.0 3.577213436586022E-5 2.1463280619516133E-4 22-23 0.0 3.577213436586022E-5 0.0 8.943033591465055E-5 2.1463280619516133E-4 24-25 0.0 3.577213436586022E-5 0.0 1.788606718293011E-4 2.1463280619516133E-4 26-27 0.0 3.577213436586022E-5 0.0 2.504049405610215E-4 2.682910077439517E-4 28-29 0.0 3.577213436586022E-5 0.0 8.22759090414785E-4 2.8617707492688174E-4 30-31 0.0 3.577213436586022E-5 0.0 0.002414619069695565 2.8617707492688174E-4 32-33 0.0 3.577213436586022E-5 0.0 0.004811352072208199 2.8617707492688174E-4 34-35 0.0 3.577213436586022E-5 0.0 0.008567426180623523 2.8617707492688174E-4 36-37 0.0 3.577213436586022E-5 0.0 0.013521866790295163 2.8617707492688174E-4 38-39 0.0 3.577213436586022E-5 0.0 0.019764104237137772 2.8617707492688174E-4 40-41 0.0 3.577213436586022E-5 0.0 0.026775442572846374 2.8617707492688174E-4 42-43 0.0 3.577213436586022E-5 0.0 0.03596888110487245 2.8617707492688174E-4 44-45 0.0 3.577213436586022E-5 0.0 0.0469866984895574 3.2194920929274197E-4 46-47 0.0 7.154426873172043E-5 0.0 0.060651653817316006 3.577213436586022E-4 48-49 0.0 7.154426873172043E-5 0.0 0.07540765924323334 3.756074108415323E-4 50-51 0.0 7.154426873172043E-5 0.0 0.0933831567620781 4.2926561239032266E-4 52-53 0.0 7.154426873172043E-5 0.0 0.11704642364509464 4.2926561239032266E-4 54-55 0.0 7.154426873172043E-5 0.0 0.14695192797495377 5.365820154879032E-4 56-57 0.0 7.154426873172043E-5 0.0 0.17769807746241065 5.365820154879032E-4 58-59 0.0 7.154426873172043E-5 0.0 0.21418565451558808 5.365820154879032E-4 60-61 0.0 7.154426873172043E-5 0.0 0.26031382178036483 5.365820154879032E-4 62-63 0.0 7.154426873172043E-5 0.0 0.30227453539151883 5.723541498537635E-4 64-65 0.0 7.154426873172043E-5 0.0 0.34584499504913657 6.081262842196237E-4 66-67 0.0 7.154426873172043E-5 0.0 0.4078917621067212 6.260123514025538E-4 68-69 0.0 7.154426873172043E-5 0.0 0.4787921324198561 6.438984185854839E-4 70-71 0.0 7.154426873172043E-5 0.0 0.5452567580716243 6.438984185854839E-4 72-73 0.0 7.154426873172043E-5 0.0 0.625726174327627 6.617844857684141E-4 74-75 0.0 7.154426873172043E-5 0.0 0.7159792693326923 7.512148216830646E-4 76-77 0.0 7.154426873172043E-5 0.0 0.8157835242134424 7.869869560489249E-4 78-79 0.0 7.154426873172043E-5 0.0 0.9328657199929028 7.869869560489249E-4 80-81 0.0 7.154426873172043E-5 0.0 1.0672079706038908 7.869869560489249E-4 82-83 0.0 7.154426873172043E-5 0.0 1.2086331038193192 8.943033591465054E-4 84-85 0.0 7.154426873172043E-5 0.0 1.3523655397013457 8.943033591465054E-4 86-87 0.0 7.154426873172043E-5 0.0 1.5149498903941803 9.479615606952958E-4 88-89 0.0 7.154426873172043E-5 0.0 1.6958674599495183 0.0010016197622440862 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9715 0.0 37.617695 1 GTATCAA 14010 0.0 30.52903 1 ATCAACG 17595 0.0 24.197206 3 TATCAAC 17660 0.0 24.135052 2 TCAACGC 17790 0.0 23.931974 4 CAACGCA 18025 0.0 23.619963 5 AACGCAG 18340 0.0 23.23021 6 GTACATG 10380 0.0 21.243656 1 GAGTACT 12550 0.0 21.17655 12-13 TACATGG 10085 0.0 20.966724 2 AGAGTAC 17770 0.0 20.662882 10-11 ACGCAGA 20860 0.0 20.311846 7 CGCAGAG 20890 0.0 20.259937 8 GTACTTT 12985 0.0 20.245834 14-15 AGTACTT 13030 0.0 20.086582 12-13 TAACGCC 190 8.233112E-6 20.007042 4 ACATGGG 10435 0.0 19.48937 3 GCAGAGT 22350 0.0 18.468903 9 CCTATAC 710 0.0 18.069738 3 TACTTTT 14630 0.0 17.742134 14-15 >>END_MODULE