FastQCFastQC Report
Thu 26 May 2016
SRR937862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771916
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115650.41722043525128466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92820.33485863207976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60760.21919856157257292No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38710.1396506964857521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128700.036.436721
GGTATCA80950.035.956341
ATCAACG156800.029.7428863
TCAACGC157200.029.730384
TATCAAC157150.029.5864242
CAACGCA160050.029.1696825
AACGCAG164050.028.6436796
ACGCAGA185600.024.9590237
CGCAGAG184750.024.8175858
GTACATG103250.022.7089881
TACATGG100200.022.6317772
GCAGAGT195650.022.3902959
GAGTACT106550.021.97900212-13
AGAGTAC154450.021.69839310-11
ACATGGG102300.021.1908973
GTACTTT112950.020.73324814-15
AGTACTT111400.020.29720712-13
CAGAGTA187600.018.83903110-11
CATGGGG60200.018.085834
GTATAGG7900.018.0607221
Produced by FastQC (version 0.11.5)