##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937862_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2771916 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42913674151742 31.0 31.0 34.0 30.0 34.0 2 31.546980139369303 33.0 31.0 34.0 30.0 34.0 3 31.593879468208993 33.0 31.0 34.0 30.0 34.0 4 35.213003207889415 37.0 35.0 37.0 33.0 37.0 5 35.183965170661736 37.0 35.0 37.0 33.0 37.0 6 35.267835677560214 37.0 35.0 37.0 33.0 37.0 7 35.19126517542379 37.0 35.0 37.0 32.0 37.0 8 35.17541873563268 37.0 35.0 37.0 32.0 37.0 9 36.742359797338736 39.0 37.0 39.0 33.0 39.0 10-11 36.61996864262842 39.0 37.0 39.0 32.0 39.0 12-13 36.47957261331152 39.0 37.0 39.0 32.0 39.0 14-15 37.550800962222525 40.0 37.0 41.0 32.0 41.0 16-17 37.46827320885626 40.0 37.0 41.0 32.0 41.0 18-19 37.41220206528625 40.0 37.0 41.0 32.0 41.0 20-21 37.2365905027425 40.0 37.0 41.0 31.5 41.0 22-23 37.06122822625217 39.0 36.0 41.0 31.0 41.0 24-25 36.893138717046256 39.0 36.0 41.0 31.0 41.0 26-27 36.98912196473486 39.0 36.0 41.0 31.0 41.0 28-29 36.927162475341966 39.0 36.0 41.0 31.0 41.0 30-31 36.704550751177166 39.0 36.0 41.0 30.0 41.0 32-33 36.4482617799385 39.0 35.5 40.0 30.0 41.0 34-35 36.16452482687066 39.0 35.0 40.0 30.0 41.0 36-37 36.145821879162284 39.0 35.0 40.0 30.0 41.0 38-39 36.08142779218418 39.0 35.0 40.0 29.5 41.0 40-41 35.873431049137125 39.0 35.0 40.0 28.5 41.0 42-43 35.606999815290216 38.5 35.0 40.0 27.0 41.0 44-45 35.36770576741864 38.0 35.0 40.0 26.5 41.0 46-47 35.00195496544629 38.0 34.0 40.0 25.0 41.0 48-49 34.647369905870164 38.0 34.0 40.0 24.0 41.0 50-51 33.743518021469626 37.5 33.0 39.5 23.0 40.5 52-53 33.85556037773151 38.0 33.0 39.5 23.0 40.5 54-55 34.133217240349275 38.0 33.5 40.0 23.0 41.0 56-57 33.82195113416135 38.0 33.0 40.0 21.5 41.0 58-59 33.556751900129726 37.5 33.0 40.0 20.5 41.0 60-61 33.21107115078523 37.0 32.5 40.0 18.5 41.0 62-63 32.80964268036983 36.5 32.0 39.5 17.5 41.0 64-65 32.40660575572997 36.0 31.0 39.0 14.0 41.0 66-67 31.95701601347227 35.5 31.0 39.0 10.0 40.0 68-69 31.433725084021304 35.0 30.5 38.5 7.5 40.0 70-71 30.96201634537266 35.0 30.0 38.0 7.0 40.0 72-73 30.46281976077197 35.0 29.5 37.0 2.0 39.0 74-75 29.943796998177433 34.0 29.0 36.5 2.0 39.0 76-77 29.447486684300678 34.0 29.0 36.0 2.0 39.0 78-79 28.878538887902806 34.0 28.0 35.5 2.0 37.5 80-81 28.33198841523336 34.0 26.5 35.0 2.0 37.0 82-83 27.7787290451803 33.0 26.0 35.0 2.0 36.5 84-85 27.2580938599871 33.0 25.5 35.0 2.0 36.0 86-87 26.67886653130903 32.5 25.0 35.0 2.0 36.0 88-89 26.128975950209167 32.0 23.5 35.0 2.0 35.0 90-91 25.549382268438148 32.0 21.5 34.5 2.0 35.0 92-93 24.915440799793355 31.5 18.5 34.0 2.0 35.0 94-95 24.183149489378465 31.0 11.5 34.0 2.0 35.0 96-97 23.303718799559583 31.0 2.0 34.0 2.0 35.0 98-99 22.26025463975099 30.5 2.0 34.0 2.0 35.0 100-101 19.759569373675106 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3471334964801329 1101 2 0.4025749540156873 1101 3 0.35221278597223105 1101 4 0.28997098758161854 1101 5 0.38250618450469176 1101 6 0.27647289008809395 1101 7 0.29242648577051966 1101 8 0.4049366653987647 1101 9 0.4458647703279226 1101 10-11 0.3466492804481831 1101 12-13 0.5064918022062628 1101 14-15 0.7750839531708351 1101 16-17 0.7544304654945435 1101 18-19 0.6991007552669828 1101 20-21 0.8038773837244122 1101 22-23 0.7835484744834602 1101 24-25 0.7411331777958594 1101 26-27 0.6809982088368756 1101 28-29 0.7265528301332083 1101 30-31 0.7289341894176218 1101 32-33 0.5908736173577367 1101 34-35 0.6833432035880165 1101 36-37 0.6898128876900316 1101 38-39 0.7493291082191078 1101 40-41 0.7829958007179769 1101 42-43 0.754743707498946 1101 44-45 0.8489790847145287 1101 46-47 0.9169221732432575 1101 48-49 0.8408807306950763 1101 50-51 0.8922784675914244 1101 52-53 0.825507923335465 1101 54-55 0.965723487158435 1101 56-57 1.1140150131466058 1101 58-59 1.141178099401138 1101 60-61 1.248016271880914 1101 62-63 1.196536831584197 1101 64-65 1.1553790045500492 1101 66-67 1.2408702732466246 1101 68-69 1.3743987582154773 1101 70-71 1.397641977500328 1101 72-73 1.4113256933445761 1101 74-75 1.4799152548052597 1101 76-77 1.5091342750742278 1101 78-79 1.4404293774345476 1101 80-81 1.4506566696210221 1101 82-83 1.5490053198968958 1101 84-85 1.7536964761024016 1101 86-87 1.8168544366010444 1101 88-89 1.831143155427359 1101 90-91 1.8616251528738808 1101 92-93 2.0445303014484395 1101 94-95 2.1223480897554623 1101 96-97 2.198469213222399 1101 98-99 2.6399213636457617 1101 100-101 2.956843784848296 1102 1 0.11901137993297084 1102 2 0.1827985005356787 1102 3 0.2015463900761887 1102 4 0.046329670244851684 1102 5 0.1338525964610966 1102 6 0.15626856627304875 1102 7 0.15689988525678444 1102 8 0.2191351435322133 1102 9 0.25982927551407187 1102 10-11 0.08480755542328211 1102 12-13 0.23885036128958603 1102 14-15 0.38711956930399083 1102 16-17 0.3628814477853055 1102 18-19 0.3743934004159257 1102 20-21 0.20553238753667102 1102 22-23 0.4119198105242461 1102 24-25 0.3646727150167166 1102 26-27 0.3742742432880348 1102 28-29 0.30969724569629165 1102 30-31 0.31632609937910416 1102 32-33 0.2819340046074572 1102 34-35 0.4220255628943619 1102 36-37 0.5690896287068412 1102 38-39 0.5041147215579045 1102 40-41 0.5737948491024056 1102 42-43 0.5476763304487164 1102 44-45 0.5305933322729786 1102 46-47 0.6153119545909576 1102 48-49 0.8089208801011907 1102 50-51 0.6345725880383881 1102 52-53 0.6643124308374837 1102 54-55 0.6065545419871015 1102 56-57 0.7173066596090862 1102 58-59 0.7524907604773219 1102 60-61 0.7774385897854685 1102 62-63 0.7758493278871583 1102 64-65 0.84742577050514 1102 66-67 0.8288216209396033 1102 68-69 0.843659950015649 1102 70-71 0.937179826298042 1102 72-73 0.8269864835602583 1102 74-75 0.7553479446313567 1102 76-77 0.8292664729291879 1102 78-79 0.8636808503777686 1102 80-81 0.9103220192322397 1102 82-83 0.9031618687720631 1102 84-85 0.8995378550030644 1102 86-87 1.0557853372185555 1102 88-89 1.0280425052387692 1102 90-91 1.285981686452267 1102 92-93 1.5056261136843325 1102 94-95 1.491451214807661 1102 96-97 1.7512487628565019 1102 98-99 1.9840183944750542 1102 100-101 1.8925803456268149 1103 1 0.2565256020223785 1103 2 0.22206606638564708 1103 3 0.21942287607350508 1103 4 0.14704279034987877 1103 5 0.09032426144496242 1103 6 0.1905261837196619 1103 7 0.2031854642113302 1103 8 0.08612344660338778 1103 9 0.20154409211655633 1103 10-11 0.2879650184801008 1103 12-13 0.20069953510769523 1103 14-15 0.2912371833549656 1103 16-17 0.19971711895438204 1103 18-19 0.22236198319109945 1103 20-21 0.30300234857335084 1103 22-23 0.28322661822646467 1103 24-25 0.26015632373199793 1103 26-27 0.2237175135977978 1103 28-29 0.35321897207440855 1103 30-31 0.2995217747825123 1103 32-33 0.2854658410001676 1103 34-35 0.2648907150774704 1103 36-37 0.47105853422386446 1103 38-39 0.4402870536897794 1103 40-41 0.5484483191600518 1103 42-43 0.4654664355392768 1103 44-45 0.23880724451338864 1103 46-47 0.5410345280522719 1103 48-49 0.5738856302267763 1103 50-51 0.3676378029194538 1103 52-53 0.3948646209941913 1103 54-55 0.3241641952126386 1103 56-57 0.6387275504270562 1103 58-59 0.6298277701359112 1103 60-61 0.7551248239083677 1103 62-63 0.6856794486413946 1103 64-65 0.8127149807260352 1103 66-67 0.9517177159674866 1103 68-69 0.976019975157314 1103 70-71 1.0594776593496675 1103 72-73 1.0324301148218105 1103 74-75 1.0687721551484373 1103 76-77 1.0110264277309078 1103 78-79 1.0424249231804446 1103 80-81 1.0729618625186035 1103 82-83 0.9171774682818672 1103 84-85 1.0850057363614312 1103 86-87 1.142884663005301 1103 88-89 1.2828680426021464 1103 90-91 1.4236560178173043 1103 92-93 1.433349842816206 1103 94-95 1.4907826008757645 1103 96-97 1.6936371198301359 1103 98-99 1.8241484116178626 1103 100-101 1.876349050024963 1104 1 0.20107030893671762 1104 2 0.2591784791526166 1104 3 0.29744366447606296 1104 4 0.23928851668649287 1104 5 0.2636283894799405 1104 6 0.2681097824811971 1104 7 0.2969629934599496 1104 8 0.3621279173182401 1104 9 0.3152957084537249 1104 10-11 0.377896297513864 1104 12-13 0.3393241861404377 1104 14-15 0.4891379120355879 1104 16-17 0.3968731968270589 1104 18-19 0.44746904430726175 1104 20-21 0.5666385908686706 1104 22-23 0.5602842546673301 1104 24-25 0.41895234748400867 1104 26-27 0.3280455505437132 1104 28-29 0.391086163368648 1104 30-31 0.4766486192567996 1104 32-33 0.4367717430837388 1104 34-35 0.3708078672892512 1104 36-37 0.35200631546887706 1104 38-39 0.48270838180025777 1104 40-41 0.5520484599411972 1104 42-43 0.6046839386736025 1104 44-45 0.5033906334613647 1104 46-47 0.4126512843814538 1104 48-49 0.4508502906917684 1104 50-51 0.2929750852437394 1104 52-53 0.34426571567771447 1104 54-55 0.38666422448350346 1104 56-57 0.41243449239907903 1104 58-59 0.5274353569568078 1104 60-61 0.5479024369887426 1104 62-63 0.4910890206357763 1104 64-65 0.34808390368464615 1104 66-67 0.40745653836077267 1104 68-69 0.4668928510877137 1104 70-71 0.46396261154917084 1104 72-73 0.5603888255736784 1104 74-75 0.3559366115135383 1104 76-77 0.4594464215582654 1104 78-79 0.50993713147286 1104 80-81 0.7352784102987258 1104 82-83 0.7974445262882917 1104 84-85 0.8022103078617242 1104 86-87 0.7616907723265989 1104 88-89 0.7343954900094296 1104 90-91 0.9128995603132175 1104 92-93 1.0201118482029834 1104 94-95 1.0621697916642 1104 96-97 0.9841577735867126 1104 98-99 1.3238273102278022 1104 100-101 1.2025215589221325 1105 1 0.021392402436994473 1105 2 0.08184960461619184 1105 3 0.09365733823170785 1105 4 0.14534038678805672 1105 5 0.07994861061480663 1105 6 0.11429169371168513 1105 7 0.10698947823794214 1105 8 0.10137050571936612 1105 9 0.056253793354109405 1105 10-11 0.19058858198717843 1105 12-13 0.1903924154288248 1105 14-15 0.1681916014506868 1105 16-17 0.24385871823783845 1105 18-19 0.2765633915839416 1105 20-21 0.20229487232534638 1105 22-23 0.2566692722682049 1105 24-25 0.27243283651256434 1105 26-27 0.17400221996511078 1105 28-29 0.1965568753019724 1105 30-31 0.14941703317487054 1105 32-33 0.13854598539086993 1105 34-35 0.23579489701980094 1105 36-37 0.2641884215278125 1105 38-39 0.22910885762072297 1105 40-41 0.30715807609714574 1105 42-43 0.16213351995251202 1105 44-45 0.2091672626456571 1105 46-47 0.14918462542865285 1105 48-49 0.006128460895915566 1105 50-51 0.07647425905220473 1105 52-53 0.06100927707771575 1105 54-55 0.12277749527355297 1105 56-57 0.15712324480642792 1105 58-59 0.2300003466505629 1105 60-61 0.24726762096213406 1105 62-63 0.3284403667585778 1105 64-65 0.1984555412360507 1105 66-67 0.25393618038865995 1105 68-69 0.3144597136042293 1105 70-71 0.2909999980307312 1105 72-73 0.2738198460128274 1105 74-75 0.16588620536940013 1105 76-77 0.20797085903685897 1105 78-79 0.21945803414141452 1105 80-81 0.3241192679280651 1105 82-83 0.46178129320623995 1105 84-85 0.44570877736078174 1105 86-87 0.3642401665976358 1105 88-89 0.3611183611770592 1105 90-91 0.38076109857071216 1105 92-93 0.4699349476308896 1105 94-95 0.7505000707033531 1105 96-97 0.6997146993950345 1105 98-99 1.0382581413266294 1105 100-101 1.0315733160597844 1106 1 -0.07303155554713925 1106 2 0.09402253822018025 1106 3 0.041219883634983745 1106 4 0.046421290367781864 1106 5 0.04588487155815102 1106 6 0.045869658461263896 1106 7 0.02943434360888375 1106 8 -0.047228771118874135 1106 9 0.1373585814272218 1106 10-11 -0.28500436564311826 1106 12-13 -0.4454742349674987 1106 14-15 -0.2220257652633535 1106 16-17 -0.3692152882716684 1106 18-19 -0.275318711979061 1106 20-21 -0.22599326168375455 1106 22-23 -0.2797645777169748 1106 24-25 -0.30874747610190667 1106 26-27 -0.2853111899713596 1106 28-29 0.046471558849582095 1106 30-31 0.03891211790975291 1106 32-33 0.07984942014646634 1106 34-35 -0.10449846269342089 1106 36-37 -0.0583477588407888 1106 38-39 -0.019199438355961718 1106 40-41 -0.030549853476962596 1106 42-43 -0.022247479306876983 1106 44-45 -0.01610919031055147 1106 46-47 0.23871125679873728 1106 48-49 0.42317469265547913 1106 50-51 0.38821985629137146 1106 52-53 0.2539760227423642 1106 54-55 0.2082231734759361 1106 56-57 0.13125049074746897 1106 58-59 -0.05306437753861104 1106 60-61 -0.1175852684285772 1106 62-63 0.16368106609983357 1106 64-65 0.252643142825697 1106 66-67 0.5027931433057624 1106 68-69 0.4084909096958782 1106 70-71 0.33089529428002606 1106 72-73 0.3529548832072358 1106 74-75 0.40597643720117915 1106 76-77 0.6300774064547312 1106 78-79 0.6115717980948894 1106 80-81 0.56855176098518 1106 82-83 0.29090479034213246 1106 84-85 0.3461315548312065 1106 86-87 0.13255441084605835 1106 88-89 0.427406628182478 1106 90-91 0.7173040535249484 1106 92-93 0.5400949042676935 1106 94-95 0.5105410070614198 1106 96-97 0.5587832772782022 1106 98-99 0.9033599989104246 1106 100-101 0.725227780657054 1107 1 0.2115612974112686 1107 2 0.3172408992812734 1107 3 0.4023944289631949 1107 4 0.3886562593335299 1107 5 0.29153258942383786 1107 6 0.3516896730050121 1107 7 0.2865637146696969 1107 8 0.150452974801631 1107 9 0.22107341856481355 1107 10-11 0.37081325289620537 1107 12-13 0.33353778458869243 1107 14-15 0.42845565192534707 1107 16-17 0.38249965189411483 1107 18-19 0.5018376021514968 1107 20-21 0.6280834799419139 1107 22-23 0.5184011011968721 1107 24-25 0.5229420783970085 1107 26-27 0.42878630105261095 1107 28-29 0.49286730929613753 1107 30-31 0.5048988584212779 1107 32-33 0.5748698640542003 1107 34-35 0.6843831183819731 1107 36-37 0.5887655949304573 1107 38-39 0.753619868168478 1107 40-41 0.6774228512406424 1107 42-43 0.6456786470847575 1107 44-45 0.5453916908200753 1107 46-47 0.5671475164920778 1107 48-49 0.67166185546278 1107 50-51 0.6120454247733704 1107 52-53 0.7218872340064593 1107 54-55 0.7121485475221832 1107 56-57 0.7611103963688564 1107 58-59 0.8457484022697841 1107 60-61 0.9175088885552398 1107 62-63 0.8040063596403257 1107 64-65 0.8283154916168556 1107 66-67 0.881985342394568 1107 68-69 1.0697158310170778 1107 70-71 0.9834700406330121 1107 72-73 1.0264122457095262 1107 74-75 1.0208635314827532 1107 76-77 0.9219378200290542 1107 78-79 0.9775888480623642 1107 80-81 1.1920993330539957 1107 82-83 1.333704617350616 1107 84-85 1.3118325119151741 1107 86-87 1.2481739636797506 1107 88-89 1.306339759293035 1107 90-91 1.2960837205522147 1107 92-93 1.5147805784374455 1107 94-95 1.6062225684895814 1107 96-97 1.3957119350485065 1107 98-99 1.4593536638747295 1107 100-101 1.6230057860552556 1108 1 0.18455914926487438 1108 2 0.20971105958026826 1108 3 0.1725008075767427 1108 4 0.19241311713340536 1108 5 0.10517993625787625 1108 6 0.12428783208343219 1108 7 0.046836818801921254 1108 8 0.13609616751238462 1108 9 0.20787038029867944 1108 10-11 0.19085100294258694 1108 12-13 0.0722666621605299 1108 14-15 0.12808710929559197 1108 16-17 0.12349370562608897 1108 18-19 0.1480837784567015 1108 20-21 0.09895982773473122 1108 22-23 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88-89 0.12452258445799913 2316 90-91 -0.14355177424207355 2316 92-93 0.08481124249248495 2316 94-95 0.212445398116472 2316 96-97 0.3629008501213171 2316 98-99 0.5536522245908877 2316 100-101 0.6247325990450818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12612.0 3 2828.0 4 2714.0 5 3175.0 6 3637.0 7 4451.0 8 5317.0 9 6721.0 10 8423.0 11 10350.0 12 13165.0 13 16071.0 14 18313.0 15 21387.0 16 25644.0 17 30779.0 18 28480.0 19 22909.0 20 23475.0 21 25106.0 22 27347.0 23 30092.0 24 33949.0 25 38351.0 26 44192.0 27 51277.0 28 60480.0 29 71913.0 30 86113.0 31 104253.0 32 130511.0 33 163992.0 34 213583.0 35 285127.0 36 377064.0 37 449138.0 38 289798.0 39 29176.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50443559348845 18.808277457802404 14.752913895514236 22.934373053194907 2 15.290983046039603 19.796348411158686 40.67485142037992 24.23781712242179 3 19.483092400905484 23.852798770501142 29.63293395604209 27.031174872551283 4 12.807996734963249 16.3054285577829 36.90540800657639 33.98116670067747 5 13.840606217835926 37.0653881181391 34.205623572822375 14.888382091202597 6 32.338091884238644 35.85172324526326 17.183787278167696 14.626397592330399 7 28.699411663055084 32.74720165778413 20.277307199806806 18.276079479353978 8 26.361396250452806 35.041686941947745 19.494239892064407 19.102676915535046 9 26.1192521530303 17.053222033927106 20.45016312013401 36.37736269290859 10-11 25.05719357306035 26.712105766576467 26.856691842384183 21.374008817979 12-13 25.787910400787872 23.917912882765513 27.716802916332767 22.577373800113847 14-15 22.76082324428068 25.376417638965542 26.098512587446336 25.76424652930744 16-17 22.01177697314186 28.69624580287858 26.82781395787669 22.464163266102872 18-19 21.940286852739433 27.147129701195666 29.318878386556374 21.59370505950853 20-21 22.65898642830962 26.1639280620164 29.618264127809425 21.558821381864554 22-23 22.377624147215418 26.012984449605092 29.55211276566824 22.057278637511242 24-25 22.18707229942673 26.243779475867296 29.64870080245936 21.92044742224661 26-27 21.851802848204454 26.42422875288208 29.611377779320186 22.11259061959328 28-29 21.851926773443793 26.76133180541493 29.585016131542712 21.80172528959857 30-31 21.987392937586545 26.403896005293014 29.490384719676342 22.118326337444095 32-33 21.81893091456171 26.428266552026543 29.485079398710624 22.26772313470112 34-35 22.364287596879326 26.50381913424762 29.342346781800476 21.789546487072574 36-37 22.339318414970318 26.216023949965912 29.373319587599056 22.071338047464714 38-39 21.88686282950271 26.735092705138065 29.322267051662525 22.0557774136967 40-41 22.28630174588692 26.567106675781645 29.25846474063063 21.888126837700806 42-43 22.02769491314049 26.49137821187702 29.08826205970411 22.392664815278383 44-45 22.06995150641008 26.66064789460461 29.10000379562202 22.169396803363284 46-47 22.3967891131183 26.97392238874599 28.52038895456937 22.108899543566345 48-49 22.296068881126402 27.010793904911896 28.370187064343128 22.322950149618574 50-51 22.342935524153226 27.22191812914548 28.34478081075472 22.090365535946574 52-53 22.493065536443883 27.181506023911222 28.139737599645905 22.185690839998994 54-55 22.392409273136753 27.17444428779548 28.156199503302282 22.27694693576549 56-57 22.36866833785827 27.418963109457266 28.082958182622498 22.12941037006196 58-59 22.494908442317666 27.00784470342467 28.177430168405415 22.319816685852246 60-61 22.223840397632678 27.19034597353187 28.40748893186088 22.17832469697457 62-63 22.14590521934211 27.249705679593273 28.56011138547887 22.044277715585743 64-65 22.13693172495694 27.250639136628873 28.329166386581345 22.28326275183284 66-67 22.1023088361278 27.34741972795151 28.125488450037018 22.42478298588367 68-69 22.22453523752318 27.168966851960874 28.329666842672673 22.27683106784327 70-71 22.31750435229816 27.120844083820007 28.115864194563724 22.445787369318115 72-73 22.2835886110054 27.270529279682197 28.04536125395602 22.400520855356383 74-75 22.144217931986258 27.661123771445983 27.838296166530125 22.356362130037635 76-77 22.33849895011193 27.39534687871773 27.865740830263668 22.40041334090667 78-79 22.38447940799441 27.378360877114126 27.900211650022566 22.336948064868896 80-81 22.385636003888198 27.407114520221576 27.777682402037417 22.42956707385281 82-83 22.46748542185164 27.439024720745465 27.803552957862266 22.289936899540635 84-85 22.3908126802444 27.495977304517595 27.725974670297965 22.38723534494004 86-87 22.452279821543048 27.50587400326241 27.710045756154607 22.331800419039936 88-89 22.50826680736803 27.48515588578615 27.544080604534006 22.462496702311814 90-91 22.386242933044493 27.4162093904176 27.660975515436437 22.536572161101468 92-93 22.506169908194945 27.29054566599411 27.713286923047175 22.489997502763764 94-95 22.474921464407586 27.157476850408784 27.775371491195973 22.592230193987657 96-97 22.487388944556532 27.08842963333746 27.779162190415867 22.64501923169014 98-99 22.3901426090351 27.130542116743868 27.630586394039007 22.848728880182026 100-101 22.806775042777666 26.551352171768368 28.028956911551866 22.6129158739021 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3896.0 1 2276.5 2 700.5 3 737.5 4 614.0 5 613.0 6 1085.5 7 2140.0 8 3385.0 9 3975.0 10 3588.0 11 3016.0 12 3004.5 13 3257.0 14 3419.0 15 3578.5 16 3858.5 17 4099.0 18 4286.5 19 4760.0 20 5438.0 21 6368.5 22 7617.0 23 9320.5 24 11290.0 25 13659.5 26 17474.0 27 21778.5 28 24532.0 29 27641.0 30 32322.0 31 37614.0 32 44169.0 33 49047.0 34 52875.5 35 58315.0 36 64639.5 37 70504.5 38 74507.5 39 77841.0 40 81011.0 41 85032.0 42 88683.5 43 91853.0 44 95330.5 45 98720.0 46 101292.0 47 104723.5 48 108331.5 49 107100.5 50 101795.5 51 97016.0 52 95017.0 53 93823.0 54 92502.5 55 85916.0 56 77023.0 57 69814.5 58 63843.5 59 57251.0 60 47553.0 61 39323.5 62 32003.0 63 24174.5 64 18021.5 65 13748.5 66 9993.0 67 7582.5 68 5991.5 69 4563.0 70 3514.5 71 2426.5 72 1668.0 73 1214.5 74 849.0 75 604.0 76 416.5 77 271.0 78 171.0 79 120.5 80 88.0 81 52.5 82 35.5 83 28.5 84 23.0 85 17.5 86 15.5 87 13.0 88 12.0 89 9.5 90 5.5 91 6.0 92 5.0 93 6.0 94 5.5 95 4.0 96 3.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33828586436241215 2 0.29142297241330545 3 0.2836666046157243 4 0.2917837337062162 5 0.2942729866273004 6 0.21963147512406583 7 0.20974661569831118 8 0.21003522473263977 9 0.22233718482089646 10-11 0.21968558931800242 12-13 0.2156089867081109 14-15 0.2166551944575521 16-17 0.2015032201553005 18-19 0.207726352458011 20-21 0.19560477301620974 22-23 0.20505671889047145 24-25 0.20455165308039636 26-27 0.20498456663188927 28-29 0.2186754576978523 30-31 0.21667323252219764 32-33 0.2222109183683777 34-35 0.21555487251417432 36-37 0.19776934077367422 38-39 0.185178771651089 40-41 0.1818778058209556 42-43 0.19926650013925387 44-45 0.20098011628057996 46-47 0.20121461112097191 48-49 0.2182425441463594 50-51 0.21465296928189745 52-53 0.23671352234338994 54-55 0.20368582597741058 56-57 0.18139077807552612 58-59 0.18330281292795308 60-61 0.17096477671040536 62-63 0.17852272579688563 64-65 0.17684518578485062 66-67 0.1757809399707639 68-69 0.17911798193018835 70-71 0.179911656774592 72-73 0.17781924127570967 74-75 0.1948652123657427 76-77 0.18732530134390799 78-79 0.19194304589316558 80-81 0.18332085099259862 82-83 0.16246884826235716 84-85 0.16209004890480086 86-87 0.16732108765200676 88-89 0.17410339995872892 90-91 0.17998380903317415 92-93 0.17522175996675224 94-95 0.17527587416068885 96-97 0.18032653226143938 98-99 0.18979651620034663 100-101 0.16959388379734452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2771916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.64652149523927 #Duplication Level Percentage of deduplicated Percentage of total 1 75.32108823354038 39.653932907318975 2 13.321676812573472 14.026798893315595 3 4.597783543667483 7.2617193048644255 4 2.1555597417696926 4.539308891174021 5 1.1883058062742526 3.1280083586467535 6 0.7393627265175188 2.335492540462996 7 0.5066434361010136 1.867111018437973 8 0.34942458004350174 1.4716790931380135 9 0.24262951158360793 1.1496239817269238 >10 1.4271615952881895 13.824735103451161 >50 0.09908907193881197 3.6148889294055992 >100 0.04743181047963202 4.440840370417307 >500 0.002882347666783261 1.0236323159715797 >1k 7.549005793677165E-4 0.6895030042865093 >5k 1.372546507941303E-4 0.5548829983144105 >10k+ 6.862732539706515E-5 0.4178422890679878 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11565 0.41722043525128466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9282 0.33485863207976 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6076 0.21919856157257292 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3871 0.1396506964857521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.60761292910752E-5 2 0.0 0.0 0.0 0.0 3.60761292910752E-5 3 0.0 0.0 0.0 0.0 3.60761292910752E-5 4 0.0 0.0 0.0 0.0 3.60761292910752E-5 5 0.0 0.0 0.0 0.0 3.60761292910752E-5 6 0.0 0.0 0.0 0.0 3.60761292910752E-5 7 0.0 0.0 0.0 0.0 3.60761292910752E-5 8 0.0 0.0 0.0 0.0 3.60761292910752E-5 9 0.0 0.0 0.0 0.0 3.60761292910752E-5 10-11 0.0 0.0 0.0 5.41141939366128E-5 3.60761292910752E-5 12-13 0.0 0.0 0.0 7.21522585821504E-5 9.0190323227688E-5 14-15 0.0 0.0 0.0 1.082283878732256E-4 1.082283878732256E-4 16-17 0.0 0.0 0.0 1.262664525187632E-4 1.262664525187632E-4 18-19 0.0 0.0 0.0 1.443045171643008E-4 1.443045171643008E-4 20-21 0.0 0.0 0.0 1.80380646455376E-4 1.623425818098384E-4 22-23 0.0 0.0 0.0 3.7879935755628957E-4 2.164567757464512E-4 24-25 0.0 0.0 0.0 5.952561333027408E-4 2.164567757464512E-4 26-27 0.0 0.0 0.0 9.560174262134928E-4 2.344948403919888E-4 28-29 0.0 0.0 0.0 0.0016414638827439215 2.525329050375264E-4 30-31 0.0 0.0 0.0 0.0033550800240699933 2.525329050375264E-4 32-33 0.0 0.0 0.0 0.00631332262593816 2.525329050375264E-4 34-35 0.0 0.0 0.0 0.010552267817639495 2.886090343286016E-4 36-37 0.0 0.0 0.0 0.014791213009340831 2.886090343286016E-4 38-39 0.0 0.0 0.0 0.020689660148431627 2.886090343286016E-4 40-41 0.0 0.0 0.0 0.029023246014669997 2.886090343286016E-4 42-43 0.0 0.0 0.0 0.03959355189695503 2.886090343286016E-4 44-45 0.0 0.0 0.0 0.051570826821591996 3.0664709897413917E-4 46-47 0.0 0.0 0.0 0.06684906757636234 3.427232282652144E-4 48-49 0.0 0.0 0.0 0.08288490704624527 3.968374222018272E-4 50-51 0.0 0.0 0.0 0.10261854976846341 4.329135514929024E-4 52-53 0.0 0.0 0.0 0.1292427331852769 4.689896807839776E-4 54-55 0.0 0.0 0.0 0.16364132246431712 5.411419393661279E-4 56-57 0.0 0.0 0.0 0.1972281988343081 5.411419393661279E-4 58-59 0.0 0.0 0.0 0.23772365396354 5.411419393661279E-4 60-61 0.0 0.0 0.0 0.288590996263956 5.411419393661279E-4 62-63 0.0 0.0 0.0 0.3332893204555982 6.132941979482783E-4 64-65 0.0 0.0 0.0 0.3792863853017191 6.313322625938159E-4 66-67 0.0 0.0 0.0 0.44615349094272694 6.493703272393536E-4 68-69 0.0 0.0 0.0 0.5230677985912993 6.493703272393536E-4 70-71 0.0 0.0 0.0 0.5948232197512479 6.493703272393536E-4 72-73 0.0 0.0 0.0 0.6804679506882604 7.034845211759664E-4 74-75 0.0 0.0 0.0 0.776520644925748 7.756367797581167E-4 76-77 0.0 0.0 0.0 0.8803477450254625 7.936748444036544E-4 78-79 0.0 0.0 0.0 0.9996695426556937 7.936748444036544E-4 80-81 0.0 0.0 0.0 1.1340350862003032 7.936748444036544E-4 82-83 0.0 0.0 0.0 1.2791873924029442 8.477890383402672E-4 84-85 0.0 0.0 0.0 1.4298593463871199 9.019032322768799E-4 86-87 0.0 0.0 0.0 1.5978117663017206 9.019032322768799E-4 88-89 0.0 0.0 0.0 1.7903320302635435 9.019032322768799E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12870 0.0 36.43672 1 GGTATCA 8095 0.0 35.95634 1 ATCAACG 15680 0.0 29.742886 3 TCAACGC 15720 0.0 29.73038 4 TATCAAC 15715 0.0 29.586424 2 CAACGCA 16005 0.0 29.169682 5 AACGCAG 16405 0.0 28.643679 6 ACGCAGA 18560 0.0 24.959023 7 CGCAGAG 18475 0.0 24.817585 8 GTACATG 10325 0.0 22.708988 1 TACATGG 10020 0.0 22.631777 2 GCAGAGT 19565 0.0 22.390295 9 GAGTACT 10655 0.0 21.979002 12-13 AGAGTAC 15445 0.0 21.698393 10-11 ACATGGG 10230 0.0 21.190897 3 GTACTTT 11295 0.0 20.733248 14-15 AGTACTT 11140 0.0 20.297207 12-13 CAGAGTA 18760 0.0 18.839031 10-11 CATGGGG 6020 0.0 18.08583 4 GTATAGG 790 0.0 18.060722 1 >>END_MODULE