FastQCFastQC Report
Thu 26 May 2016
SRR937862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771916
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126010.45459530519683855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108810.3925443628161892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76220.27497225745657516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98200.036.778591
GTATCAA140750.032.1426351
GGACCGA7050.026.2753836
ATCAACG177400.025.3363633
TATCAAC178050.025.297242
TCAACGC178900.025.1504864
CAACGCA181450.024.8232215
AACGCAG185000.024.3650916
AGGACCG9000.023.756195
TACCGTA1452.3363902E-522.930397
GAGTACT120900.021.96174812-13
ACGCAGA210400.021.3563397
CGCAGAG210350.021.2259318
GTACTTT125150.021.12067814-15
AGAGTAC175050.020.8120410-11
AGTACTT126050.020.78184312-13
GTACATG102700.020.4986971
TACATGG100600.020.402932
GTACCGT2601.4932084E-820.0952266
ACCGAGT10000.019.9494428
Produced by FastQC (version 0.11.5)