##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937862_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2771916 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.765147284405444 31.0 31.0 34.0 30.0 34.0 2 31.989000748940445 31.0 31.0 34.0 30.0 34.0 3 32.08093607454194 33.0 31.0 34.0 30.0 34.0 4 35.815082058763686 37.0 35.0 37.0 35.0 37.0 5 35.68784443684441 37.0 35.0 37.0 33.0 37.0 6 35.72003588853342 37.0 35.0 37.0 33.0 37.0 7 35.64568839748391 37.0 35.0 37.0 33.0 37.0 8 35.617923486858906 37.0 35.0 37.0 33.0 37.0 9 37.213002125605534 39.0 37.0 39.0 33.0 39.0 10-11 37.12278077690666 39.0 37.0 39.0 33.0 39.0 12-13 36.99930589527244 39.0 37.0 39.0 33.0 39.0 14-15 38.086468529349375 40.0 37.0 41.0 33.0 41.0 16-17 38.02989773138869 40.0 37.0 41.0 33.0 41.0 18-19 38.004362686315176 40.0 37.0 41.0 33.0 41.0 20-21 37.87376800018471 40.0 37.0 41.0 33.0 41.0 22-23 37.688497234403926 39.0 37.0 41.0 32.0 41.0 24-25 37.77170736775573 39.0 37.0 41.0 32.0 41.0 26-27 37.604305830335406 39.0 37.0 41.0 32.0 41.0 28-29 37.43405031032687 39.0 36.0 41.0 32.0 41.0 30-31 37.471388382620546 39.0 36.5 41.0 32.0 41.0 32-33 37.381482339291665 39.0 36.0 41.0 32.0 41.0 34-35 37.23070468224867 39.0 36.0 41.0 31.0 41.0 36-37 37.02736157949953 39.0 36.0 41.0 31.0 41.0 38-39 36.86144475518017 39.0 35.0 40.5 31.0 41.0 40-41 36.65262096686912 39.0 35.0 40.0 30.0 41.0 42-43 36.40521862855873 39.0 35.0 40.0 30.0 41.0 44-45 36.07516732108765 38.5 35.0 40.0 29.5 41.0 46-47 35.72480425092247 38.0 35.0 40.0 28.5 41.0 48-49 35.37161497678862 38.0 34.5 40.0 27.0 41.0 50-51 35.08470693195609 38.0 34.0 40.0 26.5 41.0 52-53 34.787205312137885 38.0 34.0 40.0 26.0 41.0 54-55 34.51919682991837 38.0 33.5 40.0 25.5 41.0 56-57 34.19149930950289 38.0 33.0 40.0 24.5 41.0 58-59 33.8726314217314 37.0 33.0 40.0 23.5 41.0 60-61 33.45155498940083 36.5 32.0 39.5 22.5 41.0 62-63 33.039850774698806 36.0 32.0 39.0 22.0 40.0 64-65 32.58520929205647 36.0 31.0 39.0 20.5 40.0 66-67 32.075466752960764 35.0 31.0 38.5 19.5 40.0 68-69 31.57056400699011 35.0 30.0 38.0 18.0 40.0 70-71 31.109248079667637 34.5 30.0 37.0 15.5 39.5 72-73 30.547625000180382 34.0 29.0 36.5 12.0 39.0 74-75 29.978002760545415 34.0 29.0 36.0 8.0 39.0 76-77 28.843341933882556 32.5 27.0 35.0 8.0 37.0 78-79 29.465971191046194 34.0 29.0 35.0 7.0 37.0 80-81 29.246263054147384 34.0 29.0 35.0 4.5 37.0 82-83 28.81534252841717 33.5 28.5 35.0 2.0 36.5 84-85 28.334854844086184 33.0 27.5 35.0 2.0 36.0 86-87 27.811751510507534 33.0 26.5 35.0 2.0 35.5 88-89 27.292357524542588 32.5 26.0 35.0 2.0 35.0 90-91 26.586458428033175 32.0 25.0 34.0 2.0 35.0 92-93 25.92865819166237 32.0 24.0 34.0 2.0 35.0 94-95 25.250484682797026 31.0 21.0 34.0 2.0 35.0 96-97 24.44708064746551 31.0 17.5 34.0 2.0 35.0 98-99 23.27939320672055 31.0 2.0 34.0 2.0 35.0 100-101 20.55151382653731 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31869386262689403 1101 2 0.274158739986067 1101 3 0.23195756699725223 1101 4 0.14769982020957428 1101 5 0.08397959281317213 1101 6 0.13820513227620523 1101 7 0.1766094996515548 1101 8 0.1448207759878315 1101 9 0.20817242246548773 1101 10-11 0.3220086062848395 1101 12-13 0.3010814213730555 1101 14-15 0.38358749257354674 1101 16-17 0.30517483982111315 1101 18-19 0.3450413996781734 1101 20-21 0.402580986812076 1101 22-23 0.30933051065763806 1101 24-25 0.24640468658608228 1101 26-27 0.2823191334775146 1101 28-29 0.3765132674114966 1101 30-31 0.5341953286398677 1101 32-33 0.4269446929719223 1101 34-35 0.3597418702960269 1101 36-37 0.30502394278690304 1101 38-39 0.14729492450844361 1101 40-41 0.1692548063518302 1101 42-43 0.3865512148023882 1101 44-45 0.5379015810330614 1101 46-47 0.5498010786705336 1101 48-49 0.4515544526108002 1101 50-51 0.44803405183586875 1101 52-53 0.3740093079368805 1101 54-55 0.3323205416896471 1101 56-57 0.44488190113262505 1101 58-59 0.40833803607940666 1101 60-61 0.2977138812247233 1101 62-63 0.40566484286350857 1101 64-65 0.43021846200421265 1101 66-67 0.5142782894811759 1101 68-69 0.4617456828410553 1101 70-71 0.5326047306829658 1101 72-73 0.4389143686459107 1101 74-75 0.3810733125893613 1101 76-77 0.540453663948913 1101 78-79 0.589632193412367 1101 80-81 0.597905865260973 1101 82-83 0.44092102633828034 1101 84-85 0.34881826798674 1101 86-87 0.2340907892572055 1101 88-89 0.5237140861171632 1101 90-91 0.7173272504979593 1101 92-93 0.7455706305263696 1101 94-95 0.5888535042715262 1101 96-97 0.9799401455642958 1101 98-99 1.1793775641701068 1101 100-101 1.3759158928567707 1102 1 0.3000029029622979 1102 2 0.3087475523115053 1102 3 0.2528627923805189 1102 4 0.1390070281964526 1102 5 0.12107240547317843 1102 6 0.18644475272511585 1102 7 0.22543976678385746 1102 8 0.19873364385942693 1102 9 0.2743575042348283 1102 10-11 0.2767661755231856 1102 12-13 0.33352403740411063 1102 14-15 0.3648480554222857 1102 16-17 0.3518830238130164 1102 18-19 0.21892558545545882 1102 20-21 0.2682347377450398 1102 22-23 0.30837716194965736 1102 24-25 0.17074131425349037 1102 26-27 0.20933872629425565 1102 28-29 0.2396125310937336 1102 30-31 0.22944876730749542 1102 32-33 0.16795020395791482 1102 34-35 0.13914437648161737 1102 36-37 0.20122899702956687 1102 38-39 0.2112206539847321 1102 40-41 -0.051511102772479944 1102 42-43 0.2521937254891 1102 44-45 0.3437834700324274 1102 46-47 0.2523343036453838 1102 48-49 0.205590862719788 1102 50-51 0.09757811856199083 1102 52-53 0.33288627044306196 1102 54-55 0.29412587092274123 1102 56-57 0.20332599484789426 1102 58-59 0.0760409404208886 1102 60-61 0.0740249429206088 1102 62-63 0.21359967806956348 1102 64-65 0.21545911442076005 1102 66-67 0.12137824440716116 1102 68-69 0.03332181277779611 1102 70-71 -0.0774077667126285 1102 72-73 0.1508947380267749 1102 74-75 0.03510531451238208 1102 76-77 -0.06619630435861978 1102 78-79 0.11717870887951065 1102 80-81 0.3322528804229954 1102 82-83 0.2674167734435393 1102 84-85 0.33372983864600414 1102 86-87 0.4732053014951987 1102 88-89 0.640317826243173 1102 90-91 0.7710816874124902 1102 92-93 0.5729012327938356 1102 94-95 0.532397514984801 1102 96-97 0.9248692183544982 1102 98-99 0.8079147409392426 1102 100-101 1.2818240425879033 1103 1 0.35105907746687137 1103 2 0.34284271741991645 1103 3 0.2442154496117297 1103 4 0.1403865513435676 1103 5 0.21056850403368088 1103 6 0.20331912888470782 1103 7 0.22278681916909449 1103 8 0.072954079906971 1103 9 0.2493545033123823 1103 10-11 0.22751367856946558 1103 12-13 0.27830057592207424 1103 14-15 0.357817005277262 1103 16-17 0.34891033827519635 1103 18-19 0.24968843496078819 1103 20-21 0.15033621494386296 1103 22-23 0.24650775368350253 1103 24-25 0.2714625449597179 1103 26-27 0.2044238221213064 1103 28-29 0.18052091362236666 1103 30-31 0.4286719279162625 1103 32-33 0.30809640625601276 1103 34-35 0.3360396143090796 1103 36-37 0.21642940517904918 1103 38-39 0.2512580060068075 1103 40-41 0.22795347979410252 1103 42-43 0.3137579348358557 1103 44-45 0.3932837308333106 1103 46-47 0.45095019049877294 1103 48-49 0.4505615389913302 1103 50-51 0.4031131172480755 1103 52-53 0.3941674498470391 1103 54-55 0.4696291877981622 1103 56-57 0.44861502723635027 1103 58-59 0.4002463628444346 1103 60-61 0.2552343981753893 1103 62-63 0.5193571475427632 1103 64-65 0.513149659778783 1103 66-67 0.45443353431549127 1103 68-69 0.4568666597764093 1103 70-71 0.5845270550542061 1103 72-73 0.4084063863520697 1103 74-75 0.5407797915077772 1103 76-77 0.515716922782314 1103 78-79 0.5494539271718359 1103 80-81 0.5425515776441614 1103 82-83 0.6154617160270917 1103 84-85 0.630076847894852 1103 86-87 0.7038368553866583 1103 88-89 0.7620067033406492 1103 90-91 0.7445972701603836 1103 92-93 0.9088834884806012 1103 94-95 0.8252316125770811 1103 96-97 0.8355159264537235 1103 98-99 0.9715901578031207 1103 100-101 1.2250828172671149 1104 1 0.23438763379501992 1104 2 0.2347362801244337 1104 3 0.12518161296002006 1104 4 0.11071327039805823 1104 5 0.11334157895913677 1104 6 0.10660303894774614 1104 7 0.14520076165488405 1104 8 0.19436869164182724 1104 9 0.2491398893295269 1104 10-11 0.24535514896913213 1104 12-13 0.3184261900833647 1104 14-15 0.24804815846973582 1104 16-17 0.3417640321881308 1104 18-19 0.23952898602112072 1104 20-21 0.24209838658915572 1104 22-23 0.31071942606150316 1104 24-25 0.07264622361901019 1104 26-27 0.17556251497345698 1104 28-29 0.11225832914079348 1104 30-31 0.30489872841400967 1104 32-33 0.34717295261239656 1104 34-35 0.29960095831480515 1104 36-37 0.2655647086991877 1104 38-39 0.27715358910351995 1104 40-41 0.33312106369260164 1104 42-43 0.4282604074043732 1104 44-45 0.44284779398532237 1104 46-47 0.35383502158714464 1104 48-49 0.2999314426410038 1104 50-51 0.2956679087950249 1104 52-53 0.3144042839290009 1104 54-55 0.3521255734317421 1104 56-57 0.4619928077929032 1104 58-59 0.36091035953986506 1104 60-61 0.4305997805301658 1104 62-63 0.4369897527574764 1104 64-65 0.36278627955118026 1104 66-67 0.32448956811795426 1104 68-69 0.28656777146045087 1104 70-71 0.43274930662535027 1104 72-73 0.47943301862429877 1104 74-75 0.3796484795969697 1104 76-77 0.32957201748175535 1104 78-79 0.48561522415684166 1104 80-81 0.5555128923321853 1104 82-83 0.7281738068613777 1104 84-85 0.644932046096077 1104 86-87 0.7320165026282446 1104 88-89 0.7157446279379691 1104 90-91 0.6221512702231422 1104 92-93 0.5816362705007023 1104 94-95 0.9050431339642486 1104 96-97 1.042359958171165 1104 98-99 1.2926276788371638 1104 100-101 1.5920216210207165 1105 1 0.17953187773487045 1105 2 0.18998061832283142 1105 3 0.13153297463250624 1105 4 0.0430605781753286 1105 5 -0.04383547116437114 1105 6 0.002267374228303254 1105 7 0.06263786202217858 1105 8 0.02972904880902405 1105 9 0.1625988992076941 1105 10-11 0.03889719189633212 1105 12-13 0.09569354044848666 1105 14-15 0.16011850114370674 1105 16-17 0.21142585848073026 1105 18-19 0.2003628654991374 1105 20-21 0.19520041221046114 1105 22-23 0.216227457246859 1105 24-25 0.21298976425560312 1105 26-27 0.06746359700309057 1105 28-29 0.14468251640661123 1105 30-31 0.1360891965465214 1105 32-33 0.2591095222322366 1105 34-35 0.04955356606614458 1105 36-37 0.06194045365503342 1105 38-39 0.14661784288540503 1105 40-41 0.1324666847860243 1105 42-43 0.18376671211698437 1105 44-45 0.1460587245930114 1105 46-47 0.13802930076533215 1105 48-49 0.18366630856382216 1105 50-51 0.1890306043609371 1105 52-53 0.11981291144491024 1105 54-55 0.13616161166385155 1105 56-57 0.14157964571347037 1105 58-59 0.22414633429266217 1105 60-61 0.23106217795366746 1105 62-63 0.22780882801750835 1105 64-65 0.19254200309418934 1105 66-67 0.19736806468262102 1105 68-69 -0.07302454543853898 1105 70-71 0.09728593277288766 1105 72-73 0.06571898242370722 1105 74-75 0.058175023143643756 1105 76-77 0.04673690683429044 1105 78-79 0.251603733851848 1105 80-81 0.1730927182580011 1105 82-83 0.27975286627902207 1105 84-85 0.28988383977549503 1105 86-87 0.3420426672655985 1105 88-89 0.28126798471321734 1105 90-91 0.4404294943938538 1105 92-93 0.614465664350984 1105 94-95 0.6105681097364624 1105 96-97 0.9494187277609143 1105 98-99 0.9041331790820699 1105 100-101 0.9831243523234718 1106 1 0.07863246157042525 1106 2 0.10439817328836298 1106 3 0.0166627751540247 1106 4 0.05419125025691329 1106 5 -0.013312244911354298 1106 6 0.02117147542798392 1106 7 0.1201224354776187 1106 8 0.046579819399283906 1106 9 0.045013129722306644 1106 10-11 -0.07902786673516005 1106 12-13 -0.18929146090318483 1106 14-15 -0.017684150601780857 1106 16-17 0.06203751585936601 1106 18-19 0.14222713567270517 1106 20-21 0.06223088779113084 1106 22-23 0.11235716156313202 1106 24-25 0.20328691733460147 1106 26-27 0.20296993478037706 1106 28-29 0.21089862948181093 1106 30-31 0.13447210300052603 1106 32-33 0.2134173460948432 1106 34-35 0.12628284383443145 1106 36-37 0.14676268305264983 1106 38-39 0.09982907554417864 1106 40-41 0.22457886055322263 1106 42-43 0.08089183217358453 1106 44-45 0.060941652413255554 1106 46-47 0.3663956900821219 1106 48-49 0.2526083891243971 1106 50-51 0.40923702972840914 1106 52-53 0.2736085280965028 1106 54-55 0.2845677924734815 1106 56-57 0.09908675084751906 1106 58-59 0.1922225334132932 1106 60-61 0.22669597967627197 1106 62-63 0.2601183639220537 1106 64-65 0.24540535440487332 1106 66-67 0.1607907637720487 1106 68-69 0.2599172257113622 1106 70-71 0.3623450235853767 1106 72-73 0.25667804355197177 1106 74-75 -0.003181696975204318 1106 76-77 -0.056195216369662404 1106 78-79 0.10185810644857085 1106 80-81 0.16162835567256195 1106 82-83 0.08481214218948097 1106 84-85 0.31925928723840613 1106 86-87 0.5048015387712752 1106 88-89 0.06506973016420403 1106 90-91 0.3326171261476176 1106 92-93 0.5030469135611746 1106 94-95 0.8018156723275673 1106 96-97 0.6294258163275508 1106 98-99 0.7828487637140036 1106 100-101 0.8056115731368934 1107 1 0.20394013848839876 1107 2 0.29868082753413816 1107 3 0.20818714910802072 1107 4 0.14862149837514949 1107 5 0.05629443898258302 1107 6 0.17726912698424968 1107 7 0.1324491594327526 1107 8 0.13290857756217633 1107 9 0.22962994272494086 1107 10-11 0.1875976485555384 1107 12-13 0.18134221038657472 1107 14-15 0.21980523210361724 1107 16-17 0.17997688017542401 1107 18-19 0.2599289361502244 1107 20-21 0.35143371315954397 1107 22-23 0.3343670378933652 1107 24-25 0.2636686846993399 1107 26-27 0.31686951583900935 1107 28-29 0.3464892984821901 1107 30-31 0.3509900376926325 1107 32-33 0.21100223353654712 1107 34-35 0.3232099681619047 1107 36-37 0.1633094296979536 1107 38-39 0.42205704817702383 1107 40-41 0.4629568613990145 1107 42-43 0.41668005173863065 1107 44-45 0.3767841534585088 1107 46-47 0.28754132761697093 1107 48-49 0.31699435908090123 1107 50-51 0.23671770295773342 1107 52-53 0.23056299083444287 1107 54-55 0.2827343698889848 1107 56-57 0.22089865731229708 1107 58-59 0.09349684836567462 1107 60-61 0.2836009050227588 1107 62-63 0.4455727002707661 1107 64-65 0.4082907067912416 1107 66-67 0.35980229214732873 1107 68-69 0.4683366654536485 1107 70-71 0.42550088487109505 1107 72-73 0.6763555892767208 1107 74-75 0.5900114341593152 1107 76-77 0.4599186920766165 1107 78-79 0.5752592227470323 1107 80-81 0.6796216899882559 1107 82-83 0.6935010126291381 1107 84-85 0.7929245031243966 1107 86-87 0.9379808689203202 1107 88-89 0.8558101913710949 1107 90-91 0.9434002242532138 1107 92-93 1.0730355804602532 1107 94-95 1.2975098127789515 1107 96-97 1.3216645590002685 1107 98-99 1.4554829189966547 1107 100-101 1.3205727638552496 1108 1 0.09324350218397015 1108 2 0.032679621983060514 1108 3 0.04346910993743336 1108 4 0.0011964711115481919 1108 5 -0.04756812125884835 1108 6 -0.08421027953323801 1108 7 -0.01563435532079893 1108 8 -0.0295326691602682 1108 9 0.03194776783219311 1108 10-11 0.02706598087846146 1108 12-13 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52-53 -0.03152700126357644 2316 54-55 -0.04308849312435825 2316 56-57 -0.030047591274822594 2316 58-59 -0.19057086997433004 2316 60-61 0.023763874607546143 2316 62-63 0.06985807021687407 2316 64-65 0.060552756142350006 2316 66-67 -0.18528268340177334 2316 68-69 0.08979549753615856 2316 70-71 0.3652675073085092 2316 72-73 0.24104925684903478 2316 74-75 0.4278992884321191 2316 76-77 0.22983445368172184 2316 78-79 0.24583655100375168 2316 80-81 0.19383059091083155 2316 82-83 0.23385540829139373 2316 84-85 0.462555387779652 2316 86-87 0.3052455275633186 2316 88-89 0.44752933639702164 2316 90-91 0.5437820623335838 2316 92-93 0.5190789344885083 2316 94-95 0.45870578113046534 2316 96-97 0.6271616232543735 2316 98-99 0.8973586605386892 2316 100-101 1.5250193248553465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 32.0 5 67.0 6 167.0 7 469.0 8 1078.0 9 2148.0 10 3520.0 11 5357.0 12 7660.0 13 10615.0 14 13890.0 15 18137.0 16 23466.0 17 29955.0 18 28079.0 19 21114.0 20 20290.0 21 21148.0 22 23079.0 23 25982.0 24 30054.0 25 35945.0 26 43375.0 27 52992.0 28 64688.0 29 78755.0 30 95658.0 31 115940.0 32 142359.0 33 179021.0 34 231336.0 35 307286.0 36 409746.0 37 474117.0 38 242413.0 39 11959.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.526342947249155 20.221915109351826 15.781303561827714 22.470438381571302 2 15.912964173517524 21.02754917537184 39.197255616692566 23.862231034418073 3 19.736023746751343 24.23287718675458 30.18486130171333 25.84623776478075 4 13.528469539054297 17.366653318469936 36.27617779798045 32.82869934449531 5 13.595483845125969 37.5255258809609 34.213250760408734 14.665739513504406 6 32.265696363093255 36.62398139048947 16.610820818524083 14.499501427893197 7 28.387981453983453 33.03188119697711 20.192386782283446 18.387750566755994 8 26.40617536750753 35.60169211476827 19.12088966620922 18.871242851514978 9 25.9467097848564 16.95293075259135 20.270780211232953 36.8295792513193 10-11 25.07582303209153 27.09181197836153 26.574985163686478 21.25737982586046 12-13 25.79782273411922 24.13857296185726 27.662604962434497 22.400999341589024 14-15 22.608224780260297 25.59668114040974 26.127523344863263 25.6675707344667 16-17 21.775713982674798 28.96987138138385 26.906118367223247 22.348296268718098 18-19 21.779321595603907 27.281472454432244 29.54544798615831 21.39375796380554 20-21 22.399812981345754 26.154724746348734 30.0275152638103 21.417947008495204 22-23 22.19970247721003 26.03881793946618 29.908544301114564 21.852935282209224 24-25 22.108985265065755 26.278718402722163 29.968115916932547 21.64418041527954 26-27 21.782289482921648 26.51766783878645 29.90114004793101 21.798902630360896 28-29 21.742423600151596 26.794084377014087 29.866475124017793 21.597016898816527 30-31 21.88210248795418 26.429318204447753 29.807901826750882 21.880677480847183 32-33 21.75805471738682 26.39726456357263 29.78407354335413 22.06060717568642 34-35 22.225078249124433 26.523386711574233 29.635656347450645 21.61587869185069 36-37 22.28103232565489 26.22799536493891 29.68199613552503 21.80897617388117 38-39 21.878182029063307 26.686699752102744 29.612836182356993 21.822282036476956 40-41 22.202410328784836 26.52290627669645 29.500372307266055 21.77431108725266 42-43 22.030227177398622 26.490878175725346 29.352185855196396 22.126708791679633 44-45 22.116182452859324 26.667564962286015 29.294917306296437 21.921335278558225 46-47 22.472975371548056 27.000475483384058 28.67426357797278 21.852285567095105 48-49 22.328833196965565 27.0908461872582 28.518613118146437 22.061707497629797 50-51 22.384895501883896 27.24941881355712 28.417491727743556 21.94819395681543 52-53 22.60101363118753 27.247367389085277 28.15704519131265 21.994573788414545 54-55 22.517763886062923 27.201112876436373 28.207420426881626 22.073702810619082 56-57 22.445990426838332 27.442299838811856 28.12226272369004 21.989447010659774 58-59 22.571246747736947 27.08492970205446 28.181680108632438 22.162143441576152 60-61 22.31660338913589 27.29700322809205 28.382570034589794 22.00382334818227 62-63 22.23745236147127 27.289769964169185 28.527289427240945 21.9454882471186 64-65 22.2098541225636 27.27488856083662 28.385798848161343 22.129458468438436 66-67 22.16926847711114 27.38912362423681 28.14194225221832 22.29966564643373 68-69 22.27772743146247 27.201530928831797 28.34922248613953 22.1715191535662 70-71 22.406847826557517 27.183922600829174 28.101013883537597 22.308215689075713 72-73 22.378239456029693 27.27422115244473 28.01645143647932 22.331087955046254 74-75 22.183392281728594 27.70092960969957 27.766624241138622 22.349053867433213 76-77 22.324900898872837 27.40766675469242 27.945146966935507 22.322285379499235 78-79 22.372972341153194 27.466272426725773 27.912696488638183 22.248058743482847 80-81 22.41918990676303 27.45370484778486 27.84271382010022 22.28439142535189 82-83 22.47338602493928 27.475979958525926 27.835090040882417 22.21554397565238 84-85 22.364388816425674 27.572685133993613 27.732845774448577 22.330080275132133 86-87 22.40565731429091 27.572444475229407 27.703671395525692 22.318226814953988 88-89 22.44099388293152 27.54203229823703 27.60411931674697 22.41285450208448 90-91 22.351263677406063 27.496515592071535 27.69639775540268 22.455822975119723 92-93 22.443845245538267 27.337275587522985 27.787985564870237 22.43089360206851 94-95 22.381125546372978 27.24238396834536 27.800193079443964 22.576297405837696 96-97 22.344598465465765 27.210510708116697 27.814497264707878 22.630393561709663 98-99 22.196850329785498 27.268340682443576 27.603258080488768 22.93155090728216 100-101 22.29541783138373 27.003785428176275 28.04682586561315 22.653970874826847 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 860.0 1 587.0 2 434.5 3 581.0 4 490.0 5 463.0 6 1019.5 7 2294.5 8 3552.0 9 4088.5 10 3634.0 11 2984.5 12 2977.0 13 3242.0 14 3563.5 15 3795.5 16 4006.5 17 4313.5 18 4683.5 19 5148.0 20 5702.0 21 6576.5 22 7942.5 23 9777.0 24 11771.5 25 14242.5 26 18099.5 27 22384.5 28 25223.5 29 28341.5 30 32828.5 31 38247.5 32 44629.5 33 49300.0 34 53491.5 35 58756.0 36 65090.5 37 71291.5 38 75501.0 39 78413.5 40 81407.0 41 85753.5 42 89246.5 43 92527.5 44 95923.5 45 98687.0 46 101529.0 47 105759.0 48 109365.0 49 107517.5 50 102211.5 51 97089.5 52 94322.5 53 93918.0 54 93013.5 55 85945.0 56 76716.5 57 69033.0 58 62273.0 59 55474.0 60 46063.5 61 37919.0 62 30821.5 63 23184.5 64 16941.5 65 12567.5 66 9270.5 67 7368.0 68 5746.5 69 4355.0 70 3299.5 71 2133.0 72 1432.5 73 1039.5 74 730.0 75 500.0 76 356.5 77 220.0 78 128.5 79 80.5 80 51.5 81 35.5 82 22.5 83 15.0 84 8.5 85 3.5 86 1.5 87 1.5 88 3.0 89 2.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021140611764570067 2 0.0 3 0.0 4 2.164567757464512E-4 5 0.025577975667372316 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.60761292910752E-5 12-13 0.0032829277654878434 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.92093555504568E-4 24-25 0.0 26-27 5.411419393661279E-4 28-29 0.0012807025898331694 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.41141939366128E-5 40-41 4.329135514929024E-4 42-43 0.01455671816894884 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.80380646455376E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.443045171643008E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.80380646455376E-5 82-83 0.0023088722746288128 84-85 4.1487548684736476E-4 86-87 0.0 88-89 0.0 90-91 0.010209544589374281 92-93 0.002417100662502038 94-95 0.0 96-97 0.0 98-99 0.0013889309777063953 100-101 0.01800198851624652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2771916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.654154657211556 #Duplication Level Percentage of deduplicated Percentage of total 1 73.26559933713747 37.11206999876658 2 14.3834889553376 14.571669481079303 3 5.050593945545353 7.675007004852919 4 2.326326017708695 4.713523115364452 5 1.310241495172403 3.31845876673795 6 0.7987794737689834 2.4276899400780088 7 0.5214130601978609 1.8488216454086683 8 0.37216989089544494 1.5081560969740333 9 0.28316132708260017 1.2908967889484966 >10 1.5246325473988478 14.270684678532724 >50 0.10908801596950257 3.8071746331179948 >100 0.05022015023993308 4.529618591422789 >500 0.0031429079332907315 1.0810856756989256 >1k 9.285864348145351E-4 0.7197348057372779 >5k 7.142972575496423E-5 0.2757801472616897 >10k+ 1.4285945150992847E-4 0.8496286300182364 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12601 0.45459530519683855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10881 0.3925443628161892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7622 0.27497225745657516 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.082283878732256E-4 14-15 0.0 0.0 0.0 3.60761292910752E-5 1.443045171643008E-4 16-17 0.0 0.0 0.0 5.41141939366128E-5 1.80380646455376E-4 18-19 0.0 0.0 0.0 7.21522585821504E-5 1.80380646455376E-4 20-21 0.0 0.0 0.0 9.0190323227688E-5 2.164567757464512E-4 22-23 0.0 0.0 0.0 2.70570969683064E-4 2.525329050375264E-4 24-25 0.0 0.0 0.0 4.870277454295152E-4 2.525329050375264E-4 26-27 0.0 0.0 0.0 8.477890383402672E-4 2.886090343286016E-4 28-29 0.0 0.0 0.0 0.0015512735595162336 3.0664709897413917E-4 30-31 0.0 0.0 0.0 0.0033190038947789186 3.246851636196768E-4 32-33 0.0 0.0 0.0 0.006349398755229234 3.60761292910752E-4 34-35 0.0 0.0 0.0 0.010624420076221646 3.60761292910752E-4 36-37 0.0 1.80380646455376E-5 0.0 0.014971593655796207 3.60761292910752E-4 38-39 0.0 3.60761292910752E-5 0.0 0.020996307247405768 3.60761292910752E-4 40-41 0.0 3.60761292910752E-5 0.0 0.029420083436871823 3.60761292910752E-4 42-43 0.0 3.60761292910752E-5 0.0 0.04000842738380239 3.60761292910752E-4 44-45 0.0 3.60761292910752E-5 0.0 0.052003740373084896 3.60761292910752E-4 46-47 0.0 3.60761292910752E-5 0.0 0.06713767661069095 3.60761292910752E-4 48-49 0.0 3.60761292910752E-5 0.0 0.08338997285632033 3.60761292910752E-4 50-51 0.0 3.60761292910752E-5 0.0 0.10308753944924738 3.968374222018272E-4 52-53 0.0 3.60761292910752E-5 0.0 0.13007248415897163 4.329135514929024E-4 54-55 0.0 3.60761292910752E-5 0.0 0.16484987279556812 4.689896807839776E-4 56-57 0.0 3.60761292910752E-5 0.0 0.198364596906977 4.689896807839776E-4 58-59 0.0 3.60761292910752E-5 0.0 0.23905847074730982 4.689896807839776E-4 60-61 0.0 3.60761292910752E-5 0.0 0.2900701175648901 4.689896807839776E-4 62-63 0.0 3.60761292910752E-5 0.0 0.334624137239368 4.689896807839776E-4 64-65 0.0 5.41141939366128E-5 0.0 0.3798636033703763 4.689896807839776E-4 66-67 0.0 7.21522585821504E-5 0.0 0.44633387158918236 4.689896807839776E-4 68-69 0.0 7.21522585821504E-5 0.0 0.5227250753630341 4.689896807839776E-4 70-71 0.0 7.21522585821504E-5 0.0 0.5945526487815649 4.689896807839776E-4 72-73 0.0 7.21522585821504E-5 0.0 0.6805761790761337 4.870277454295152E-4 74-75 0.0 7.21522585821504E-5 0.0 0.777458624287316 5.050658100750528E-4 76-77 0.0 7.21522585821504E-5 0.0 0.8816284476152957 5.231038747205904E-4 78-79 0.0 7.21522585821504E-5 0.0 1.0013290446030831 5.411419393661279E-4 80-81 0.0 7.21522585821504E-5 0.0 1.1363619965395777 5.411419393661279E-4 82-83 0.0 7.21522585821504E-5 0.0 1.2820374066169393 6.854464565304287E-4 84-85 0.0 7.21522585821504E-5 0.0 1.432781512859697 6.854464565304287E-4 86-87 0.0 7.21522585821504E-5 0.0 1.6011307701964994 7.034845211759664E-4 88-89 0.0 7.21522585821504E-5 0.0 1.7941380619037517 7.21522585821504E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9820 0.0 36.77859 1 GTATCAA 14075 0.0 32.142635 1 GGACCGA 705 0.0 26.275383 6 ATCAACG 17740 0.0 25.336363 3 TATCAAC 17805 0.0 25.29724 2 TCAACGC 17890 0.0 25.150486 4 CAACGCA 18145 0.0 24.823221 5 AACGCAG 18500 0.0 24.365091 6 AGGACCG 900 0.0 23.75619 5 TACCGTA 145 2.3363902E-5 22.93039 7 GAGTACT 12090 0.0 21.961748 12-13 ACGCAGA 21040 0.0 21.356339 7 CGCAGAG 21035 0.0 21.225931 8 GTACTTT 12515 0.0 21.120678 14-15 AGAGTAC 17505 0.0 20.81204 10-11 AGTACTT 12605 0.0 20.781843 12-13 GTACATG 10270 0.0 20.498697 1 TACATGG 10060 0.0 20.40293 2 GTACCGT 260 1.4932084E-8 20.095226 6 ACCGAGT 1000 0.0 19.949442 8 >>END_MODULE