FastQCFastQC Report
Thu 26 May 2016
SRR937861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711527
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115290.42518477595834375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91540.3375957532416236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59690.2201342638299379No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39210.1446048665567409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84150.033.9043961
GTATCAA122750.033.4696461
ATCAACG147800.027.5590613
TCAACGC148150.027.491414
CAACGCA150500.027.0621435
TATCAAC151000.027.00952
AACGCAG155550.026.2053876
GTACATG103350.024.1550391
TACATGG101150.024.1547992
ACATGGG101600.023.1563193
ACGCAGA177650.022.8116727
GAGTACT99500.022.69846212-13
CGCAGAG177950.022.6931218
GTACTTT105650.021.22039614-15
AGTACTT103550.020.82450512-13
GTGTAGC16000.020.5063321
GCAGAGT190650.020.4557519
AGAGTAC150950.020.31084810-11
CATGGGG55700.019.453494
GAGTACA85250.019.2434791
Produced by FastQC (version 0.11.5)