##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937861_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2711527 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.523686468915855 31.0 31.0 34.0 30.0 34.0 2 31.658058724843972 33.0 31.0 34.0 30.0 34.0 3 31.67380889070992 33.0 31.0 34.0 30.0 34.0 4 35.300535454745614 37.0 35.0 37.0 33.0 37.0 5 35.28354281554268 37.0 35.0 37.0 33.0 37.0 6 35.33476598241508 37.0 35.0 37.0 33.0 37.0 7 35.265864953585194 37.0 35.0 37.0 33.0 37.0 8 35.24376043461857 37.0 35.0 37.0 32.0 37.0 9 36.836351620323164 39.0 37.0 39.0 33.0 39.0 10-11 36.709097678171744 39.0 37.0 39.0 32.0 39.0 12-13 36.57208576569586 39.0 37.0 39.0 32.0 39.0 14-15 37.66459175955099 40.0 37.0 41.0 32.0 41.0 16-17 37.577219957610595 40.0 37.0 41.0 32.0 41.0 18-19 37.50015083751701 40.0 37.0 41.0 32.0 41.0 20-21 37.401312618314336 40.0 37.0 41.0 32.0 41.0 22-23 37.18856699564489 39.5 36.5 41.0 31.5 41.0 24-25 37.00308553077288 39.0 36.0 41.0 31.0 41.0 26-27 36.9382475262094 39.0 36.0 41.0 31.0 41.0 28-29 36.98243591157308 39.0 36.0 41.0 31.0 41.0 30-31 36.795673987387914 39.0 36.0 41.0 30.0 41.0 32-33 36.53988804094519 39.0 35.5 40.5 30.0 41.0 34-35 36.53884951173269 39.0 36.0 41.0 30.0 41.0 36-37 36.423463421164534 39.0 35.0 41.0 30.0 41.0 38-39 36.27587407390743 39.0 35.0 41.0 30.0 41.0 40-41 36.03027002865913 39.0 35.0 40.5 29.5 41.0 42-43 35.759935453344184 39.0 35.0 40.0 28.0 41.0 44-45 35.5142167125756 38.5 35.0 40.0 27.0 41.0 46-47 35.164797547654885 38.0 34.5 40.0 25.5 41.0 48-49 34.788705589138516 38.0 34.0 40.0 24.0 41.0 50-51 33.848901375498016 37.5 33.0 39.5 23.5 40.5 52-53 33.95629436844995 38.0 33.5 39.5 23.0 40.5 54-55 34.287096901487615 38.0 34.0 40.0 23.0 41.0 56-57 34.05057832726725 38.0 33.5 40.0 22.5 41.0 58-59 33.776319948132546 38.0 33.0 40.0 22.0 41.0 60-61 33.4201722866857 37.0 33.0 40.0 20.5 41.0 62-63 33.02913837848563 37.0 32.0 40.0 18.5 41.0 64-65 32.628304641628134 36.0 32.0 39.0 17.5 41.0 66-67 32.19300563852029 36.0 31.0 39.0 13.5 40.5 68-69 31.669498773200488 35.0 31.0 38.5 9.5 40.0 70-71 31.196974066642156 35.0 30.5 38.0 7.5 40.0 72-73 30.717083215472314 35.0 30.0 37.0 6.0 39.5 74-75 30.23779147321786 34.5 29.5 37.0 2.0 39.0 76-77 29.723563512367754 34.0 29.0 36.0 2.0 39.0 78-79 29.162555453071278 34.0 28.5 36.0 2.0 38.0 80-81 28.609263525681286 34.0 27.5 35.0 2.0 37.0 82-83 28.048384360546656 33.5 26.5 35.0 2.0 37.0 84-85 27.54519612011977 33.0 26.0 35.0 2.0 36.0 86-87 27.03738022892636 33.0 25.5 35.0 2.0 36.0 88-89 26.490359859960826 32.0 24.5 35.0 2.0 35.0 90-91 25.927503580086054 32.0 23.5 35.0 2.0 35.0 92-93 25.292646541966942 32.0 20.0 34.0 2.0 35.0 94-95 24.57880024797835 31.0 17.0 34.0 2.0 35.0 96-97 23.758356638159974 31.0 4.5 34.0 2.0 35.0 98-99 22.721457872261645 31.0 2.0 34.0 2.0 35.0 100-101 20.354381866748884 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1845002018367694 1101 2 0.24942585512898674 1101 3 0.18647039517971464 1101 4 0.1467642033363461 1101 5 0.028803459965402567 1101 6 0.0846133707786052 1101 7 0.12392517417129056 1101 8 0.014150683837506506 1101 9 0.2921468259178184 1101 10-11 0.25510116205485645 1101 12-13 0.30451729800713423 1101 14-15 0.4687464221482003 1101 16-17 0.49956383825002604 1101 18-19 0.45023916742325554 1101 20-21 0.5449297362356162 1101 22-23 0.5433465149392944 1101 24-25 0.4897772194588157 1101 26-27 0.39928963969313 1101 28-29 0.3077802526404483 1101 30-31 0.4485812713159234 1101 32-33 0.38537070002266915 1101 34-35 0.5886623650965106 1101 36-37 0.5150582505146488 1101 38-39 0.553811173645002 1101 40-41 0.5593178858781442 1101 42-43 0.47880519775117847 1101 44-45 0.5448381018169215 1101 46-47 0.7392324247420987 1101 48-49 0.621962742018944 1101 50-51 0.5965725113708515 1101 52-53 0.6053297948028202 1101 54-55 0.6774772444314223 1101 56-57 0.6909069840229236 1101 58-59 0.5689768564002833 1101 60-61 0.7635135834412949 1101 62-63 0.888950899447245 1101 64-65 0.8812067143152333 1101 66-67 0.9076008359266723 1101 68-69 0.9051512636239494 1101 70-71 0.9603546673950412 1101 72-73 0.8966661775209666 1101 74-75 0.9772870767776496 1101 76-77 0.9920226789535782 1101 78-79 1.1211477056388937 1101 80-81 1.0932685472211645 1101 82-83 1.0821765743357403 1101 84-85 1.2579586770500413 1101 86-87 1.163149911547361 1101 88-89 1.2577892148308045 1101 90-91 1.3950285162914398 1101 92-93 1.5341913777143041 1101 94-95 1.7652575980255492 1101 96-97 1.8666638505576003 1101 98-99 2.158657674432032 1101 100-101 2.340969262856177 1102 1 0.20439694475007641 1102 2 0.13006195244028973 1102 3 0.14450972538033113 1102 4 0.16846193326372827 1102 5 0.10463635266745541 1102 6 0.08414834296157636 1102 7 0.030836712337922734 1102 8 0.22385034756364774 1102 9 0.3016011053188734 1102 10-11 0.15474676505253626 1102 12-13 0.211418567224392 1102 14-15 0.3478281739388649 1102 16-17 0.2936587691480952 1102 18-19 0.39411213964805114 1102 20-21 0.45896854763647355 1102 22-23 0.5403568192387596 1102 24-25 0.5482361078133025 1102 26-27 0.5404553309963163 1102 28-29 0.38450498803762656 1102 30-31 0.5718315668127119 1102 32-33 0.5657958295483496 1102 34-35 0.6288486849141677 1102 36-37 0.6734187685576316 1102 38-39 0.5657232171684186 1102 40-41 0.48085688896604495 1102 42-43 0.4851140668373759 1102 44-45 0.5741836473582822 1102 46-47 0.4893026270142613 1102 48-49 0.6606244029150901 1102 50-51 0.5339067767513725 1102 52-53 0.47909540485061086 1102 54-55 0.4649404255801741 1102 56-57 0.5172625973667309 1102 58-59 0.40933744962441665 1102 60-61 0.4827153860962312 1102 62-63 0.7062250273707278 1102 64-65 0.6019604352846031 1102 66-67 0.6999071647859765 1102 68-69 0.6993318313362806 1102 70-71 0.757755888814998 1102 72-73 0.7878496061003624 1102 74-75 0.8818287281342485 1102 76-77 0.9522901222266604 1102 78-79 0.9480330100628294 1102 80-81 0.9893720472774241 1102 82-83 0.928485440932505 1102 84-85 0.9744450583071327 1102 86-87 1.1079442885943038 1102 88-89 1.2402617986030258 1102 90-91 1.1615046410463528 1102 92-93 1.338213893226019 1102 94-95 1.536680306817562 1102 96-97 1.8035974402039088 1102 98-99 1.969065920560201 1102 100-101 2.3184261183321055 1103 1 0.10477601253260715 1103 2 0.23222336915421593 1103 3 0.24588923423909037 1103 4 0.16770723465626958 1103 5 0.15158232942216188 1103 6 0.1883712182057451 1103 7 0.1684224214682004 1103 8 0.2966221965895528 1103 9 0.17624165683600523 1103 10-11 0.18567795143302135 1103 12-13 0.18498299299469778 1103 14-15 0.29405876228872074 1103 16-17 0.2319686022837999 1103 18-19 0.21888064623268377 1103 20-21 0.24094657402084607 1103 22-23 0.3073747716139863 1103 24-25 0.28571806378587183 1103 26-27 0.2045636123456589 1103 28-29 0.1558305939446143 1103 30-31 0.29209472320001595 1103 32-33 0.22186342018378724 1103 34-35 0.25355296335355604 1103 36-37 0.23486369417613417 1103 38-39 0.45349187268788427 1103 40-41 0.5407129377250186 1103 42-43 0.5542692340167292 1103 44-45 0.4965241582047426 1103 46-47 0.732152323129931 1103 48-49 0.8214962158529389 1103 50-51 0.7223451377808487 1103 52-53 0.5658957691681152 1103 54-55 0.6473522962589087 1103 56-57 0.5648204241784427 1103 58-59 0.6495474906960652 1103 60-61 0.80390516057453 1103 62-63 0.9873269875822146 1103 64-65 0.9076266179130741 1103 66-67 0.845486664479516 1103 68-69 0.9322691269089063 1103 70-71 0.9319881584099541 1103 72-73 1.000093552758777 1103 74-75 0.9316900325742239 1103 76-77 1.0310984860629908 1103 78-79 1.040871679272847 1103 80-81 0.8993144495962255 1103 82-83 1.0616199319477246 1103 84-85 1.212394225704152 1103 86-87 1.3540533661721632 1103 88-89 1.4260565664078975 1103 90-91 1.5104575146293229 1103 92-93 1.634708053262223 1103 94-95 1.7529533702351578 1103 96-97 1.8735459717637752 1103 98-99 1.9298061982663732 1103 100-101 2.36746004683193 1104 1 0.15756591725710933 1104 2 0.28113139661139996 1104 3 0.26717108500615083 1104 4 0.2013324585230123 1104 5 0.21852125088904018 1104 6 0.05884641891326936 1104 7 0.09548388268147079 1104 8 0.25623051274447306 1104 9 0.2812872420686574 1104 10-11 0.4012125644870679 1104 12-13 0.36121250119141735 1104 14-15 0.4043880929884409 1104 16-17 0.4957014714749235 1104 18-19 0.40156389084032895 1104 20-21 0.3853997758654657 1104 22-23 0.5299501975737044 1104 24-25 0.40341957464266187 1104 26-27 0.41161609038203295 1104 28-29 0.41529189296353053 1104 30-31 0.48524283295851944 1104 32-33 0.4106243347209144 1104 34-35 0.45721445745269307 1104 36-37 0.5079730820863162 1104 38-39 0.626338036866791 1104 40-41 0.6290161526715323 1104 42-43 0.6846077987563817 1104 44-45 0.7042848681744758 1104 46-47 0.6735714164972677 1104 48-49 0.710481230892178 1104 50-51 0.5950295865310196 1104 52-53 0.6256409082978891 1104 54-55 0.6621426671325352 1104 56-57 0.6436881675948243 1104 58-59 0.5353325197836369 1104 60-61 0.7327231760974797 1104 62-63 0.87487404435457 1104 64-65 0.6476307525918585 1104 66-67 0.7426357316225491 1104 68-69 0.9108242151750687 1104 70-71 0.8392245418499833 1104 72-73 0.8804337709138288 1104 74-75 0.9957484763614097 1104 76-77 1.134815181430671 1104 78-79 1.1591969838490677 1104 80-81 1.1465554124267605 1104 82-83 1.2510378225408871 1104 84-85 1.319153433460471 1104 86-87 1.3653997297871747 1104 88-89 1.3207972914887058 1104 90-91 1.3031826237148287 1104 92-93 1.5262883033179264 1104 94-95 1.631536691994583 1104 96-97 1.8758665117669615 1104 98-99 1.7935248024736232 1104 100-101 2.386061369153744 1105 1 0.28129552333044217 1105 2 0.09070844507989761 1105 3 0.1442284958672495 1105 4 0.15031983599929077 1105 5 0.12958010676484122 1105 6 0.19760675824258556 1105 7 0.25675061513308606 1105 8 0.14170153059004775 1105 9 0.2407279528664077 1105 10-11 0.34870526600611385 1105 12-13 0.3026691632074616 1105 14-15 0.2914765774492025 1105 16-17 0.28932485473126235 1105 18-19 0.3707784710742388 1105 20-21 0.32553026540868757 1105 22-23 0.3557613055013533 1105 24-25 0.39887949016624447 1105 26-27 0.3831698104051 1105 28-29 0.486764508867239 1105 30-31 0.35604937627692124 1105 32-33 0.2832891433632341 1105 34-35 0.3884603088190488 1105 36-37 0.3961516116948829 1105 38-39 0.5395511792042953 1105 40-41 0.5472647686072634 1105 42-43 0.5658176248563294 1105 44-45 0.5778633772289581 1105 46-47 0.5909000478975059 1105 48-49 0.6480710438054871 1105 50-51 0.5304905831462676 1105 52-53 0.5291379617669918 1105 54-55 0.5624660714768268 1105 56-57 0.5112115067177072 1105 58-59 0.5538707948774189 1105 60-61 0.45505990901177995 1105 62-63 0.5379706245457712 1105 64-65 0.5330047280016927 1105 66-67 0.3700594012124654 1105 68-69 0.44777435994346604 1105 70-71 0.5561781167487254 1105 72-73 0.5138836367338904 1105 74-75 0.6452951519378125 1105 76-77 0.8397824861935241 1105 78-79 0.8056438070411964 1105 80-81 0.8384207092954234 1105 82-83 0.9406611888912622 1105 84-85 1.0171248961997357 1105 86-87 1.0508477132620406 1105 88-89 1.0719138078109651 1105 90-91 1.0674128820023085 1105 92-93 1.2101618080302359 1105 94-95 1.3463880329931364 1105 96-97 1.396093547689457 1105 98-99 1.7444487465629415 1105 100-101 2.019997837445281 1106 1 0.20586667374596956 1106 2 0.18559760228304967 1106 3 0.15059271072170688 1106 4 0.14548501243404388 1106 5 0.17148689656599458 1106 6 0.05674204301828922 1106 7 -0.005197210433635746 1106 8 -0.055388710022576504 1106 9 0.04829346324363826 1106 10-11 -0.026762403031867166 1106 12-13 -0.02411721150428292 1106 14-15 0.004624542793152386 1106 16-17 -0.12099663289002649 1106 18-19 0.080366672135888 1106 20-21 0.18702701028532687 1106 22-23 0.015997811832114905 1106 24-25 -0.023279891224348148 1106 26-27 0.21141432809208283 1106 28-29 0.30262677663036897 1106 30-31 0.3398111512845432 1106 32-33 0.37424924158801787 1106 34-35 0.24172610582117926 1106 36-37 0.14471372825929762 1106 38-39 0.21685247255155105 1106 40-41 0.28454388523694973 1106 42-43 0.27632104042435657 1106 44-45 0.3846973006723644 1106 46-47 0.5317774083378382 1106 48-49 0.562327845028129 1106 50-51 0.29477185898058167 1106 52-53 0.2958548693726186 1106 54-55 0.3428874019167196 1106 56-57 0.226885863851237 1106 58-59 0.3260301082829784 1106 60-61 0.19166930508714586 1106 62-63 0.5470747721766287 1106 64-65 0.544402350701084 1106 66-67 0.6166863918142127 1106 68-69 0.6604218194787776 1106 70-71 0.7196159702708975 1106 72-73 0.6075577654377149 1106 74-75 0.7469592895612713 1106 76-77 0.958501349048678 1106 78-79 1.106822599518246 1106 80-81 0.9546995893371921 1106 82-83 0.7942716020227074 1106 84-85 0.8741529169106954 1106 86-87 1.18101314803992 1106 88-89 1.203834344185676 1106 90-91 1.3369742153791542 1106 92-93 1.3527380464455874 1106 94-95 1.5035327840047543 1106 96-97 1.6595377918046736 1106 98-99 2.0319534375301807 1106 100-101 2.4549515260411994 1107 1 0.01077696840730269 1107 2 0.03615878076467993 1107 3 0.04120813122699474 1107 4 -0.022975215311511477 1107 5 0.003539075864807728 1107 6 -0.0661049538207763 1107 7 0.14275773157950766 1107 8 0.11063294585980543 1107 9 0.1121916857805445 1107 10-11 0.16007851750001834 1107 12-13 0.16747131854506137 1107 14-15 0.2832943717588847 1107 16-17 0.27134966204961586 1107 18-19 0.3561665578129194 1107 20-21 0.40753229601773455 1107 22-23 0.35087218615937843 1107 24-25 0.4400684077779857 1107 26-27 0.36504824354491916 1107 28-29 0.3540652270765392 1107 30-31 0.3538477755643967 1107 32-33 0.3377298032712588 1107 34-35 0.2731213372706378 1107 36-37 0.20228278247540032 1107 38-39 0.3341170156484523 1107 40-41 0.3076760756133581 1107 42-43 0.3627460736094079 1107 44-45 0.3227676706940983 1107 46-47 0.24781250194489246 1107 48-49 0.37563398913783175 1107 50-51 0.3150587305282073 1107 52-53 0.2445179180853927 1107 54-55 0.2567242338332889 1107 56-57 0.3219226368328165 1107 58-59 0.3406302302456794 1107 60-61 0.3858972225399455 1107 62-63 0.5391476060085907 1107 64-65 0.5090356235987556 1107 66-67 0.32172553856644726 1107 68-69 0.45664097580794305 1107 70-71 0.6353582306189658 1107 72-73 0.6620382575449248 1107 74-75 0.44439016031219225 1107 76-77 0.7193518001333139 1107 78-79 0.9757959164261756 1107 80-81 0.856259729635223 1107 82-83 0.878903483399661 1107 84-85 0.8667462749912715 1107 86-87 1.0254616624551218 1107 88-89 1.0870840498517573 1107 90-91 1.0515190857835535 1107 92-93 1.1249211378316666 1107 94-95 1.4804351770111346 1107 96-97 1.6399563666964525 1107 98-99 1.7433055864065246 1107 100-101 2.0815413043664925 1108 1 -0.016433956269459316 1108 2 -0.06984177318566509 1108 3 0.0709292915913089 1108 4 0.032770726247804305 1108 5 0.029120589011427 1108 6 0.06645557926482581 1108 7 -0.045888872888085075 1108 8 -0.020027235779870978 1108 9 0.03147433086111562 1108 10-11 0.007566665225851921 1108 12-13 0.10509607861666836 1108 14-15 0.0963596786247436 1108 16-17 0.10114707141568147 1108 18-19 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-0.62503338233984 2316 88-89 -0.5289771050121139 2316 90-91 -0.43314714584290215 2316 92-93 -0.5946684879143582 2316 94-95 -0.2671202402469035 2316 96-97 -0.23377824832653715 2316 98-99 -0.1465566707582049 2316 100-101 0.17471947460078496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12268.0 3 2515.0 4 2558.0 5 3023.0 6 3348.0 7 3953.0 8 4757.0 9 6068.0 10 7585.0 11 9591.0 12 12101.0 13 15096.0 14 16937.0 15 20570.0 16 23951.0 17 29547.0 18 27059.0 19 21366.0 20 21432.0 21 22909.0 22 25268.0 23 28052.0 24 31574.0 25 35600.0 26 40706.0 27 47995.0 28 55991.0 29 67112.0 30 81241.0 31 99282.0 32 123901.0 33 157180.0 34 206798.0 35 277867.0 36 374602.0 37 456350.0 38 304013.0 39 31358.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43359450296928 18.819531004598495 14.7810766523077 22.965797840124523 2 15.289831858976683 19.767353252992795 40.700233712101316 24.242581175929207 3 19.4966652956998 23.839659013909888 29.617840520060007 27.045835170330307 4 12.757034852158272 16.30530289470687 37.00332974866388 33.93433250447097 5 13.830964290178263 37.15191928365874 34.178935916390365 14.838180509772627 6 32.38251250590459 35.79032068488077 17.196792338040495 14.63037447117414 7 28.689947778266443 32.73079382209131 20.284429201299446 18.294829198342804 8 26.429224892020116 34.960753909361586 19.48779554699643 19.122225651621868 9 26.101526636575773 16.99216259283888 20.446011046962713 36.46029972362263 10-11 25.042161648267708 26.69720848766422 26.876814799057353 21.38381506501072 12-13 25.7922606954755 23.9100403381776 27.73690940478133 22.56078956156557 14-15 22.747528979191753 25.331502290466823 26.127640024512466 25.793328705828955 16-17 21.988117907261426 28.733519269776874 26.85407290995216 22.424289913009538 18-19 21.959276528192817 27.122634274171936 29.30951575733719 21.608573440298052 20-21 22.549198951053086 26.136199016094892 29.707160363524554 21.607441669327468 22-23 22.31558495091682 26.004209267594458 29.605385320183885 22.074820461304835 24-25 22.221253238448472 26.20265564784305 29.64369768642396 21.93239342728452 26-27 21.832720725129036 26.42084234105464 29.621822612735954 22.12461432108037 28-29 21.857110988594837 26.769450963004417 29.561294188335935 21.812143860064808 30-31 21.945781595045766 26.398756451480853 29.48450803710316 22.170953916370216 32-33 21.801825112625618 26.434265911978745 29.490234492318358 22.27367448307728 34-35 22.3213310538261 26.477686375311837 29.36437637298514 21.83660619787692 36-37 22.32492145273454 26.208567287530666 29.37322201585062 22.09328924388418 38-39 21.867649509779465 26.72713539074074 29.306599675465172 22.098615424014618 40-41 22.234806605335354 26.550508909989595 29.260476776085515 21.95420770858954 42-43 22.018385073150757 26.483711548084806 29.08837445145248 22.409528927311957 44-45 22.064812376715587 26.681615551274767 29.068909923269164 22.18466214874048 46-47 22.41811893819229 26.978379644891344 28.52933763409705 22.074163782819316 48-49 22.268271385340388 27.02746656194414 28.39537064843263 22.308891404282843 50-51 22.352373232140092 27.200267325221166 28.324293733936738 22.123065708702004 52-53 22.465796675162505 27.166309161233844 28.128653154661954 22.2392410089417 54-55 22.38154434890209 27.142870078681224 28.213272562063775 22.262313010352912 56-57 22.30530183176222 27.414436110004754 28.09731047442265 22.182951583810382 58-59 22.48870959131002 27.03241673213906 28.13019156979123 22.34868210675969 60-61 22.221362129007822 27.231015084474908 28.374959586908933 22.17266319960834 62-63 22.129297307684134 27.20200729640418 28.57054961379314 22.098145782118543 64-65 22.116986168154686 27.268993847422585 28.361637948234403 22.25238203618833 66-67 22.101475917270594 27.314150559598154 28.136929265862054 22.447444257269197 68-69 22.200217607350094 27.18850305986087 28.32544511308173 22.285834219707304 70-71 22.34922564471105 27.133712305256523 28.141510357683234 22.375551692349188 72-73 22.27485586152656 27.242249999399558 28.048671664686438 22.43422247438744 74-75 22.086099151150744 27.646353702463777 27.91247821710617 22.355068929279312 76-77 22.318644646602813 27.344839839829664 27.907328278778724 22.4291872347888 78-79 22.335480535013392 27.40188966314307 27.910276944479712 22.352352857363826 80-81 22.354565278006955 27.40864926131695 27.75183989389232 22.48494556678377 82-83 22.43561778745994 27.43033786861271 27.818732948818507 22.315311395108843 84-85 22.335245215306585 27.510536782850263 27.771360495803393 22.38285750603976 86-87 22.4254890885713 27.502424587432127 27.785651874512336 22.286434449484236 88-89 22.4920784973425 27.46830475038859 27.53216458986683 22.507452162402075 90-91 22.3613721648212 27.42224993107689 27.687724000097564 22.52865390400434 92-93 22.47897913628349 27.28202962127801 27.718401817549232 22.520589424889273 94-95 22.455254346147864 27.172316360170683 27.799160167877734 22.57326912580372 96-97 22.44000004433972 27.112796923710185 27.773237061946652 22.67396597000344 98-99 22.294170251134464 27.195523480111746 27.592142994397882 22.918163274355905 100-101 22.705668052843446 26.596526491002166 28.06753039458929 22.63027506156509 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4021.0 1 2366.0 2 690.0 3 683.0 4 579.0 5 593.5 6 1065.5 7 2096.0 8 3386.5 9 3980.0 10 3561.0 11 3022.0 12 3017.5 13 3239.0 14 3430.0 15 3587.5 16 3784.0 17 4035.0 18 4249.5 19 4614.5 20 5226.5 21 6169.5 22 7487.0 23 9227.5 24 11051.5 25 13404.0 26 17075.5 27 21319.0 28 24096.0 29 26890.5 30 31366.5 31 36849.5 32 43046.5 33 47653.0 34 51733.5 35 56690.0 36 62960.5 37 69072.5 38 72929.5 39 76240.0 40 79361.5 41 83554.0 42 87044.5 43 89953.5 44 93134.5 45 96143.5 46 99178.0 47 102514.5 48 105674.0 49 104973.5 50 100102.0 51 94957.0 52 92572.5 53 91798.0 54 90668.5 55 84066.5 56 75481.0 57 68467.0 58 62280.5 59 55741.0 60 46487.5 61 38370.5 62 31413.0 63 23851.5 64 17520.0 65 13235.0 66 9762.5 67 7444.5 68 5863.0 69 4492.0 70 3398.0 71 2307.0 72 1569.5 73 1120.5 74 780.0 75 555.5 76 392.0 77 234.0 78 159.5 79 128.0 80 80.5 81 49.0 82 33.0 83 26.0 84 22.0 85 19.0 86 16.0 87 11.0 88 8.0 89 6.0 90 3.0 91 5.5 92 7.5 93 3.5 94 3.0 95 4.0 96 1.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3203361058178657 2 0.3024495053893987 3 0.2890990943479449 4 0.2736096671727775 5 0.2707330592688179 6 0.22146192901638082 7 0.21231579106532958 8 0.2272520244128124 9 0.2274733019438862 10-11 0.22738110297260547 12-13 0.222217960580883 14-15 0.21998674547588867 16-17 0.21917539452861798 18-19 0.21987610671035177 20-21 0.2056037059560904 22-23 0.19750863627763987 24-25 0.19804339031106827 26-27 0.2119285553859504 28-29 0.22929884157524527 30-31 0.22288179317410448 32-33 0.2291882028097083 34-35 0.22022646280121863 36-37 0.20331717146832765 38-39 0.20283773681766767 40-41 0.1973426781293345 42-43 0.21187323600318198 44-45 0.20798243941513397 46-47 0.20840655468302546 48-49 0.2199683056816325 50-51 0.22894848548437835 52-53 0.24609749414259935 54-55 0.2162065876533776 56-57 0.18266460190143782 58-59 0.18128161733222647 60-61 0.188159660589771 62-63 0.1991313381721812 64-65 0.18509865474324985 66-67 0.19214265614909976 68-69 0.19459514878516793 70-71 0.18742206881952495 72-73 0.19134974499608523 74-75 0.19453982940239947 76-77 0.21325622057239332 78-79 0.21805056707899276 80-81 0.20639661710910492 82-83 0.2035568887936576 84-85 0.19520366199562092 86-87 0.1990206994066443 88-89 0.2065625752574103 90-91 0.20700513031955797 92-93 0.20156539101399323 94-95 0.20320653270279074 96-97 0.18992988083836154 98-99 0.19940793508602347 100-101 0.1859468852790328 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2711527.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.606558853302424 #Duplication Level Percentage of deduplicated Percentage of total 1 75.08628179372806 39.50030902257405 2 13.443529549806343 14.144356569159951 3 4.7779967154259655 7.540618962328251 4 2.1614979847254068 4.548358837790068 5 1.1859760874979497 3.1195060422785117 6 0.7152484348046969 2.257605532817144 7 0.47154299796277316 1.7364378131933904 8 0.3429636876134555 1.44337115335863 9 0.2548956681235175 1.20682655699325 >10 1.4077928412219882 13.685518278154344 >50 0.10061282962156247 3.6589646065209203 >100 0.04780040763074371 4.440904189648493 >500 0.002878201369953238 1.0315976447459403 >1k 7.722003675329997E-4 0.7013711773722857 >5k 1.4040006682418174E-4 0.5584896964722555 >10k+ 7.020003341209087E-5 0.425763916592517 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11529 0.42518477595834375 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9154 0.3375957532416236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5969 0.2201342638299379 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3921 0.1446048665567409 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.687958851230322E-5 2 0.0 0.0 0.0 0.0 3.687958851230322E-5 3 0.0 0.0 0.0 0.0 3.687958851230322E-5 4 0.0 0.0 0.0 0.0 7.375917702460644E-5 5 0.0 0.0 0.0 0.0 1.1063876553690964E-4 6 0.0 0.0 0.0 0.0 1.1063876553690964E-4 7 0.0 0.0 0.0 0.0 1.1063876553690964E-4 8 0.0 0.0 0.0 0.0 1.1063876553690964E-4 9 0.0 0.0 0.0 0.0 1.1063876553690964E-4 10-11 0.0 0.0 0.0 5.531938276845483E-5 1.1063876553690964E-4 12-13 0.0 0.0 0.0 7.375917702460644E-5 1.4751835404921287E-4 14-15 0.0 0.0 0.0 7.375917702460644E-5 2.0283773681766768E-4 16-17 0.0 0.0 0.0 1.1063876553690964E-4 2.5815711958612254E-4 18-19 0.0 0.0 0.0 1.1063876553690964E-4 2.9503670809842575E-4 20-21 0.0 0.0 0.0 1.8439794256151608E-4 3.6879588512303216E-4 22-23 0.0 0.0 0.0 3.3191629661072895E-4 3.6879588512303216E-4 24-25 0.0 0.0 0.0 4.0567547363533536E-4 4.0567547363533536E-4 26-27 0.0 0.0 0.0 5.347540334283967E-4 4.0567547363533536E-4 28-29 0.0 0.0 0.0 0.0012539060094183094 4.0567547363533536E-4 30-31 0.0 0.0 0.0 0.003061005846521167 4.0567547363533536E-4 32-33 0.0 0.0 0.0 0.0059929331332492725 4.0567547363533536E-4 34-35 0.0 0.0 0.0 0.009238336922331955 4.4255506214763856E-4 36-37 0.0 0.0 0.0 0.014124882400212133 4.4255506214763856E-4 38-39 0.0 0.0 0.0 0.020357532858791376 4.4255506214763856E-4 40-41 0.0 0.0 0.0 0.028968916776414173 4.4255506214763856E-4 42-43 0.0 0.0 0.0 0.03960867806221365 4.4255506214763856E-4 44-45 0.0 0.0 0.0 0.0516683035057368 4.6099485640379017E-4 46-47 0.0 0.0 0.0 0.06583006549446124 4.794346506599418E-4 48-49 0.0 0.0 0.0 0.08187268649731314 4.794346506599418E-4 50-51 0.0 0.0 0.0 0.10211958059056761 5.16314239172245E-4 52-53 0.0 0.0 0.0 0.12933671691264736 5.16314239172245E-4 54-55 0.0 0.0 0.0 0.16260210575074488 5.16314239172245E-4 56-57 0.0 0.0 0.0 0.1954064997324386 5.16314239172245E-4 58-59 0.0 0.0 0.0 0.2392563304735671 5.16314239172245E-4 60-61 0.0 0.0 0.0 0.2906480370654616 5.16314239172245E-4 62-63 0.0 0.0 0.0 0.3370794390024514 5.16314239172245E-4 64-65 0.0 0.0 0.0 0.38419311332691874 5.16314239172245E-4 66-67 0.0 0.0 0.0 0.45433809067731945 5.16314239172245E-4 68-69 0.0 0.0 0.0 0.5304391215724571 5.16314239172245E-4 70-71 0.0 0.0 0.0 0.600842256042444 5.347540334283966E-4 72-73 0.0 0.0 0.0 0.6836369322525647 5.531938276845482E-4 74-75 0.0 0.0 0.0 0.779265705264967 5.716336219406999E-4 76-77 0.0 0.0 0.0 0.885700197711474 5.900734161968515E-4 78-79 0.0 0.0 0.0 1.007679436715917 5.900734161968515E-4 80-81 0.0 0.0 0.0 1.1438573172975965 6.085132104530031E-4 82-83 0.0 0.0 0.0 1.294012561925439 6.638325932214579E-4 84-85 1.843979425615161E-5 0.0 0.0 1.445311073797163 6.638325932214579E-4 86-87 3.687958851230322E-5 0.0 0.0 1.6151046993078069 6.638325932214579E-4 88-89 3.687958851230322E-5 0.0 0.0 1.8094785705619012 6.638325932214579E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8415 0.0 33.904396 1 GTATCAA 12275 0.0 33.469646 1 ATCAACG 14780 0.0 27.559061 3 TCAACGC 14815 0.0 27.49141 4 CAACGCA 15050 0.0 27.062143 5 TATCAAC 15100 0.0 27.0095 2 AACGCAG 15555 0.0 26.205387 6 GTACATG 10335 0.0 24.155039 1 TACATGG 10115 0.0 24.154799 2 ACATGGG 10160 0.0 23.156319 3 ACGCAGA 17765 0.0 22.811672 7 GAGTACT 9950 0.0 22.698462 12-13 CGCAGAG 17795 0.0 22.693121 8 GTACTTT 10565 0.0 21.220396 14-15 AGTACTT 10355 0.0 20.824505 12-13 GTGTAGC 1600 0.0 20.506332 1 GCAGAGT 19065 0.0 20.455751 9 AGAGTAC 15095 0.0 20.310848 10-11 CATGGGG 5570 0.0 19.45349 4 GAGTACA 8525 0.0 19.243479 1 >>END_MODULE