FastQCFastQC Report
Thu 26 May 2016
SRR937861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711527
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121970.4498203410845623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107930.3980413988132886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71990.26549615770007085No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27470.10130822964329693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92800.035.7975161
GTATCAA134400.031.8248861
ATCAACG166700.025.3901633
TATCAAC167850.025.3294072
TCAACGC167350.025.2915464
CAACGCA169600.024.9280055
AACGCAG175750.024.0525996
GTACATG99000.023.4265481
TACATGG96650.023.3952922
GAGTACT120200.021.47723812-13
CGCAGAG199400.020.9862148
ACGCAGA200500.020.9184577
ACATGGG99700.020.916683
GTACTTT123300.020.8016614-15
AGTACTT123900.020.54834412-13
AGAGTAC168450.020.34457610-11
GTGTAAG10600.019.2790781
GGACCGA7400.019.255596
GCAGAGT213200.019.0708439
CATGGGG59650.018.7942474
Produced by FastQC (version 0.11.5)