##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937861_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2711527 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.843955085086744 31.0 31.0 34.0 30.0 34.0 2 32.073826666671586 33.0 31.0 34.0 30.0 34.0 3 32.13428485130334 33.0 31.0 34.0 30.0 34.0 4 35.83664591943949 37.0 35.0 37.0 35.0 37.0 5 35.71677803687738 37.0 35.0 37.0 33.0 37.0 6 35.75931311028804 37.0 35.0 37.0 33.0 37.0 7 35.698874471838195 37.0 35.0 37.0 33.0 37.0 8 35.65645778190665 37.0 35.0 37.0 33.0 37.0 9 37.277095894674844 39.0 37.0 39.0 34.0 39.0 10-11 37.180694863079 39.0 37.0 39.0 33.0 39.0 12-13 37.049442251543134 39.0 37.0 39.0 33.0 39.0 14-15 38.15235271490935 40.0 37.0 41.0 33.0 41.0 16-17 38.10050222623636 40.0 37.0 41.0 33.0 41.0 18-19 38.063328523005666 40.0 37.0 41.0 33.0 41.0 20-21 37.93519942821886 40.0 37.0 41.0 33.0 41.0 22-23 37.877440460670314 40.0 37.0 41.0 33.0 41.0 24-25 37.893996445545255 40.0 37.0 41.0 33.0 41.0 26-27 37.66654656214008 39.5 37.0 41.0 32.0 41.0 28-29 37.61948857599427 39.5 37.0 41.0 32.0 41.0 30-31 37.62624307263029 39.5 37.0 41.0 32.0 41.0 32-33 37.4838705275662 39.5 36.0 41.0 32.0 41.0 34-35 37.31361443201561 39.0 36.0 41.0 31.5 41.0 36-37 37.105083961915184 39.0 36.0 41.0 31.0 41.0 38-39 36.938883145917416 39.0 36.0 41.0 31.0 41.0 40-41 36.74774748693264 39.0 35.0 40.5 30.5 41.0 42-43 36.49424420262089 39.0 35.0 40.0 30.0 41.0 44-45 36.168944841781034 39.0 35.0 40.0 30.0 41.0 46-47 35.822066311712916 38.0 35.0 40.0 28.5 41.0 48-49 35.45701241403829 38.0 35.0 40.0 27.5 41.0 50-51 35.18111289321479 38.0 34.0 40.0 27.0 41.0 52-53 34.87292086709813 38.0 34.0 40.0 26.0 41.0 54-55 34.61492435811998 38.0 34.0 40.0 26.0 41.0 56-57 34.2818926383547 38.0 33.0 40.0 25.0 41.0 58-59 33.985262547634605 37.0 33.0 40.0 24.0 41.0 60-61 33.58040819803749 37.0 32.5 39.5 23.0 41.0 62-63 33.11030371447528 36.0 32.0 39.0 22.0 40.0 64-65 32.66344277597088 36.0 31.0 39.0 21.5 40.0 66-67 32.186276220004444 35.0 31.0 38.5 19.5 40.0 68-69 31.65476943434456 35.0 30.5 38.0 18.0 40.0 70-71 31.19404379893691 34.5 30.0 37.5 17.0 39.5 72-73 30.669227339429035 34.0 29.0 37.0 13.0 39.0 74-75 30.149888605202896 34.0 29.0 36.0 9.0 39.0 76-77 28.975008731242582 32.5 28.0 35.0 8.0 37.0 78-79 29.600065756306318 34.0 29.0 35.0 7.0 37.0 80-81 29.419508454092473 34.0 29.0 35.0 6.5 37.0 82-83 28.967540983364724 34.0 29.0 35.0 2.0 36.5 84-85 28.479669204842878 33.0 27.5 35.0 2.0 36.0 86-87 28.05065559000519 33.0 27.0 35.0 2.0 36.0 88-89 27.52812787776039 33.0 26.0 35.0 2.0 35.0 90-91 26.88582577271036 32.0 25.0 34.0 2.0 35.0 92-93 26.271641587931818 32.0 24.5 34.0 2.0 35.0 94-95 25.594451392149146 32.0 23.5 34.0 2.0 35.0 96-97 24.855715985863316 31.0 19.0 34.0 2.0 35.0 98-99 23.7858118322259 31.0 4.5 34.0 2.0 35.0 100-101 21.24181282354924 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20907386778841186 1101 2 0.18655010812084072 1101 3 0.28956345720038 1101 4 0.20645148910580957 1101 5 0.1707673194941819 1101 6 0.2219736458106638 1101 7 0.15010462066138786 1101 8 0.17882413650581697 1101 9 0.25578662529916585 1101 10-11 0.236750200004316 1101 12-13 0.2850029364948199 1101 14-15 0.33865002373627817 1101 16-17 0.384050993563406 1101 18-19 0.433468353703617 1101 20-21 0.38306771319924593 1101 22-23 0.311293702317343 1101 24-25 0.3478142856926283 1101 26-27 0.11455984821242282 1101 28-29 0.26943318197071875 1101 30-31 0.449702373039905 1101 32-33 0.4099097261650684 1101 34-35 0.2895053053348988 1101 36-37 0.29538729600918145 1101 38-39 0.35078911389134504 1101 40-41 0.30406148277432266 1101 42-43 0.2992278898284866 1101 44-45 0.37947067211985797 1101 46-47 0.42410613678200093 1101 48-49 0.26002358998950825 1101 50-51 0.4020682700700249 1101 52-53 0.3435558804514969 1101 54-55 0.4070805464358074 1101 56-57 0.4045173202999379 1101 58-59 0.419304844496871 1101 60-61 0.5168252453358946 1101 62-63 0.4561618774636642 1101 64-65 0.556625938918252 1101 66-67 0.4809565148954036 1101 68-69 0.4918504500700216 1101 70-71 0.551050758459386 1101 72-73 0.490172023599726 1101 74-75 0.6063718209532034 1101 76-77 0.544837927875772 1101 78-79 0.6318143658968722 1101 80-81 0.7649479757756765 1101 82-83 0.7850199236748168 1101 84-85 0.7887051380623262 1101 86-87 0.7332430868087272 1101 88-89 0.913724452950639 1101 90-91 0.8722165968388929 1101 92-93 0.9584803289140496 1101 94-95 0.9869202858480968 1101 96-97 1.1799176772366842 1101 98-99 1.2898819167951956 1101 100-101 1.6648209488517018 1102 1 0.18915783169747868 1102 2 0.22089383139044827 1102 3 0.21431296951863743 1102 4 0.14913469463117224 1102 5 0.07456929531473122 1102 6 0.1933080914268075 1102 7 0.1731018394564785 1102 8 0.12875448191095273 1102 9 0.1448651681987343 1102 10-11 0.27799888931296124 1102 12-13 0.22798400378842842 1102 14-15 0.20574666687274146 1102 16-17 0.27554547795509876 1102 18-19 0.36630033825712616 1102 20-21 0.33397130606191894 1102 22-23 0.22582928192423424 1102 24-25 0.2977452534583307 1102 26-27 0.25519584843547705 1102 28-29 0.3470139120942619 1102 30-31 0.2814453884117114 1102 32-33 0.3443821909419498 1102 34-35 0.20024460994405047 1102 36-37 0.28101879532059826 1102 38-39 0.20682280002844067 1102 40-41 0.31024158545884006 1102 42-43 0.352971836749596 1102 44-45 0.3444294693607546 1102 46-47 0.02761521727163796 1102 48-49 -0.02125855493387263 1102 50-51 -0.054678479465188445 1102 52-53 0.04723221412040601 1102 54-55 0.16534518360954564 1102 56-57 0.14914217877514346 1102 58-59 0.23257843868392314 1102 60-61 0.20708379632116447 1102 62-63 0.09809506382032396 1102 64-65 0.24440189359447118 1102 66-67 0.188739494777721 1102 68-69 0.1761503964972171 1102 70-71 0.11358697984449506 1102 72-73 0.21700819888036094 1102 74-75 0.27121540680560585 1102 76-77 0.17055426489049452 1102 78-79 0.1688347375009016 1102 80-81 0.3432418066389893 1102 82-83 0.31179636478805506 1102 84-85 0.38176957677773515 1102 86-87 0.5186544073981096 1102 88-89 0.5407730772350483 1102 90-91 0.4916390014210599 1102 92-93 0.7228894697477699 1102 94-95 0.8945821419165121 1102 96-97 0.9839186590727174 1102 98-99 1.01677628278901 1102 100-101 1.4132845273154402 1103 1 0.1581908436752748 1103 2 0.13995419582252566 1103 3 0.18546901885043354 1103 4 0.09303137395730232 1103 5 0.080036922038218 1103 6 0.1225132090566774 1103 7 0.2042277867679161 1103 8 0.1797446121143622 1103 9 0.15758119530487136 1103 10-11 0.17087106885962555 1103 12-13 0.2230161679833529 1103 14-15 0.19846747707821066 1103 16-17 0.25218956094617084 1103 18-19 0.2461722256623915 1103 20-21 0.2407345225959716 1103 22-23 0.36539911803863845 1103 24-25 0.2037680920416136 1103 26-27 0.23274857709339614 1103 28-29 0.20190126580875756 1103 30-31 0.2008748677699188 1103 32-33 0.22932251676553506 1103 34-35 0.2721042331389327 1103 36-37 0.3180731364844718 1103 38-39 0.33673335320676046 1103 40-41 0.21912461719914234 1103 42-43 0.37138605315190176 1103 44-45 0.39650116716052963 1103 46-47 0.3469454544790054 1103 48-49 0.34691299794085495 1103 50-51 0.2631974950554934 1103 52-53 0.28447036091286293 1103 54-55 0.28325610053255446 1103 56-57 0.2874850926278043 1103 58-59 0.22641661812748026 1103 60-61 0.4096756203718499 1103 62-63 0.08254068255862279 1103 64-65 0.24980901554345536 1103 66-67 0.310479755299113 1103 68-69 0.33982082376833134 1103 70-71 0.4833839272533176 1103 72-73 0.3872697083921395 1103 74-75 0.6727268805157429 1103 76-77 0.48677011728462105 1103 78-79 0.644844057384276 1103 80-81 0.824224690590114 1103 82-83 0.7669898941425366 1103 84-85 0.5729138258884205 1103 86-87 0.6865555146712836 1103 88-89 0.7533707441136634 1103 90-91 0.6245153625994782 1103 92-93 0.8989017969690494 1103 94-95 0.9178832824136087 1103 96-97 1.1351018317282815 1103 98-99 1.0921222698545954 1103 100-101 1.6287169681854472 1104 1 0.19010575184914558 1104 2 0.11339099418999865 1104 3 0.13221045638223217 1104 4 0.07732974314238561 1104 5 0.13414066484615006 1104 6 0.05390068912161894 1104 7 0.0956856270698836 1104 8 0.13354328267715943 1104 9 0.14796230869427518 1104 10-11 0.17387937265383613 1104 12-13 0.20246067970469284 1104 14-15 0.2254857100309806 1104 16-17 0.19118919445809723 1104 18-19 0.23011881646807808 1104 20-21 0.116706531299549 1104 22-23 0.21122753958795215 1104 24-25 0.015790668345594838 1104 26-27 0.19267206843166917 1104 28-29 0.19957952850476346 1104 30-31 0.30937090287979885 1104 32-33 0.35549852272056626 1104 34-35 0.23829097603147176 1104 36-37 0.14843830410772085 1104 38-39 0.25182522722882794 1104 40-41 0.36074580353950836 1104 42-43 0.393692223264857 1104 44-45 0.3820096653735874 1104 46-47 0.476670659120515 1104 48-49 0.5436981654592046 1104 50-51 0.5637930894902539 1104 52-53 0.5570139956854874 1104 54-55 0.543738754152514 1104 56-57 0.6015743811642977 1104 58-59 0.5883810056123338 1104 60-61 0.574961685181627 1104 62-63 0.4858837858665197 1104 64-65 0.606955521603048 1104 66-67 0.5895863089132121 1104 68-69 0.5808471112486906 1104 70-71 0.5985871274445174 1104 72-73 0.4825943513741464 1104 74-75 0.727572949822811 1104 76-77 0.6655096240833522 1104 78-79 0.6243374303813312 1104 80-81 0.694315842810834 1104 82-83 0.7642953971878974 1104 84-85 0.7760494198625096 1104 86-87 0.7605036060939767 1104 88-89 0.7754403047818066 1104 90-91 0.8027167111934936 1104 92-93 0.7914480407171425 1104 94-95 0.9386866689732329 1104 96-97 1.3655122801276995 1104 98-99 1.458222533420411 1104 100-101 1.6127401292283352 1105 1 0.06827383980284552 1105 2 0.13385888443294647 1105 3 0.043471627662746926 1105 4 0.10615407153087375 1105 5 0.032418392400373364 1105 6 0.05679071852019746 1105 7 0.053127652973373074 1105 8 0.06870080618534047 1105 9 0.08744964879948469 1105 10-11 0.14125848836534516 1105 12-13 0.05060873187115078 1105 14-15 0.11273279909158163 1105 16-17 0.21068855114182128 1105 18-19 0.3191939292041468 1105 20-21 0.1826968335069381 1105 22-23 0.20900952210541135 1105 24-25 0.19843398639830667 1105 26-27 0.10675508915304022 1105 28-29 0.09231449243278433 1105 30-31 0.1798237557160789 1105 32-33 0.09658174097472738 1105 34-35 0.14063047173557663 1105 36-37 0.06474957486646105 1105 38-39 0.08360842119179779 1105 40-41 0.0654019607791767 1105 42-43 0.18405114347228135 1105 44-45 0.26714117372335267 1105 46-47 0.2882921609214435 1105 48-49 0.2611666491903364 1105 50-51 0.2179940203858095 1105 52-53 0.2702043817108475 1105 54-55 0.4487155796403002 1105 56-57 0.2084392353637412 1105 58-59 0.3592620908682065 1105 60-61 0.32321202952933703 1105 62-63 0.352217590490568 1105 64-65 0.2708970661315746 1105 66-67 0.36899923165119475 1105 68-69 0.18582022187417735 1105 70-71 0.13920074928844528 1105 72-73 0.26838054303078707 1105 74-75 0.3339253977168646 1105 76-77 0.3268219581380052 1105 78-79 0.4622656071892024 1105 80-81 0.39752003388733215 1105 82-83 0.19927056989562786 1105 84-85 0.3142838058736288 1105 86-87 0.4097589875280292 1105 88-89 0.5518322881534594 1105 90-91 0.646297540368554 1105 92-93 0.7661964867034889 1105 94-95 0.8726941979091194 1105 96-97 0.9731220531963238 1105 98-99 1.0238936244065115 1105 100-101 1.3852821694574011 1106 1 -0.23301570029200747 1106 2 -0.20428574964441637 1106 3 -0.19333125381486838 1106 4 -0.16961347948510053 1106 5 -0.032096683960418204 1106 6 -0.09691419380630606 1106 7 -0.07082333797645646 1106 8 -0.06613132517261988 1106 9 -0.2750704747701107 1106 10-11 -0.4174724894225861 1106 12-13 -0.46948553617479405 1106 14-15 -0.45119169129261394 1106 16-17 -0.36011446086705234 1106 18-19 -0.3245844205543733 1106 20-21 -0.3049028325505816 1106 22-23 -0.27430776676027335 1106 24-25 -0.19592515949212697 1106 26-27 -0.2862016614887395 1106 28-29 -0.39400875464114193 1106 30-31 -0.24496698635414305 1106 32-33 -0.5064648042978632 1106 34-35 -0.6908132856068079 1106 36-37 -0.33843674763278386 1106 38-39 -0.42400284311225533 1106 40-41 -0.4698324380155796 1106 42-43 -0.639929556936714 1106 44-45 -0.5433420502900432 1106 46-47 -0.4669945591542657 1106 48-49 -0.5975996128294199 1106 50-51 -0.6117854633630415 1106 52-53 -0.6928302525840664 1106 54-55 -0.7895114522963524 1106 56-57 -0.8049484452045164 1106 58-59 -0.7405567970054463 1106 60-61 -0.8733850476581395 1106 62-63 -0.8535533869710363 1106 64-65 -1.354212797680809 1106 66-67 -1.0970867068685806 1106 68-69 -0.9698247113373277 1106 70-71 -0.8687292288475739 1106 72-73 -1.0389061607285122 1106 74-75 -1.1606746191434247 1106 76-77 -0.9994943680596187 1106 78-79 -0.7825928887643272 1106 80-81 -0.9776158002074311 1106 82-83 -0.9545506648213369 1106 84-85 -0.5840391393467534 1106 86-87 -0.4183865439517547 1106 88-89 -0.6156817439572322 1106 90-91 -0.677461460849738 1106 92-93 -0.47853514917955664 1106 94-95 -0.4320826344234767 1106 96-97 -0.47522536663572623 1106 98-99 -0.4335764349852127 1106 100-101 0.051859435806445475 1107 1 0.08262649334492522 1107 2 0.13052709763891102 1107 3 0.05838338621099837 1107 4 0.09761225109738803 1107 5 0.05523147346150381 1107 6 0.03013464993546222 1107 7 -0.004084912495805781 1107 8 -0.07263654815017162 1107 9 0.06464011620328591 1107 10-11 0.015227770191522438 1107 12-13 0.08229397327322374 1107 14-15 0.007543102591782258 1107 16-17 -0.0358062308404854 1107 18-19 0.061628269296122085 1107 20-21 0.09853633821220598 1107 22-23 0.07837500334822778 1107 24-25 0.011753238239968766 1107 26-27 0.07649910289269712 1107 28-29 0.04333713784888005 1107 30-31 0.06658447740287698 1107 32-33 0.11500670158676485 1107 34-35 0.18446798575201484 1107 36-37 0.10899760191995256 1107 38-39 0.22308737790762478 1107 40-41 0.11194750798795639 1107 42-43 0.12105602172444918 1107 44-45 0.008844751817967733 1107 46-47 -0.1252092380608545 1107 48-49 -0.056433382036154 1107 50-51 -0.1193197295609636 1107 52-53 0.0708037174382028 1107 54-55 -0.03117903982231951 1107 56-57 0.03182692378722152 1107 58-59 0.13180105301318434 1107 60-61 0.12363682513775842 1107 62-63 0.04141885764739328 1107 64-65 0.06690646246671861 1107 66-67 0.04584252611701345 1107 68-69 0.24253090133424493 1107 70-71 0.33764987051110396 1107 72-73 -0.005769754862843257 1107 74-75 0.27122790346537684 1107 76-77 0.1558110093494527 1107 78-79 0.3257505058095589 1107 80-81 0.43077425123790647 1107 82-83 0.4623598590316469 1107 84-85 0.4581283724641203 1107 86-87 0.5468197762808167 1107 88-89 0.6603106354204336 1107 90-91 0.654730689063431 1107 92-93 0.5084416492586712 1107 94-95 0.5206615263053571 1107 96-97 0.6636972416198113 1107 98-99 0.7162778646091965 1107 100-101 1.136198893195214 1108 1 0.01833699932575783 1108 2 0.05098381568788568 1108 3 -0.07666970788188365 1108 4 0.05422931729445679 1108 5 -0.07560243735248662 1108 6 -0.00761652720901651 1108 7 0.036311000342159616 1108 8 1.210576203973801E-4 1108 9 -0.019325176759927842 1108 10-11 -0.032405870329185404 1108 12-13 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52-53 -0.0634026438496349 2316 54-55 0.08135739386277407 2316 56-57 -0.08526190164897685 2316 58-59 0.19344030020705816 2316 60-61 0.09868548733314242 2316 62-63 0.10585219351784048 2316 64-65 0.19481360190196284 2316 66-67 0.30901377898804583 2316 68-69 0.4678511371961811 2316 70-71 0.5312347436480032 2316 72-73 0.5788579723467961 2316 74-75 0.49367947632603304 2316 76-77 0.5385638009196718 2316 78-79 0.46738729610823526 2316 80-81 0.4714459406415834 2316 82-83 0.29762685470214123 2316 84-85 0.3158991849968622 2316 86-87 0.6939764657516498 2316 88-89 0.7367351869111332 2316 90-91 0.6593566757391116 2316 92-93 0.9229340009830835 2316 94-95 0.6211914937109206 2316 96-97 1.0549976779285828 2316 98-99 0.8374088143251157 2316 100-101 1.076304097559646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 20.0 4 25.0 5 84.0 6 187.0 7 474.0 8 1154.0 9 2053.0 10 3350.0 11 5263.0 12 7484.0 13 10247.0 14 13568.0 15 17664.0 16 23096.0 17 29012.0 18 27013.0 19 20522.0 20 18901.0 21 19877.0 22 21472.0 23 23939.0 24 27874.0 25 33319.0 26 40083.0 27 49114.0 28 59623.0 29 73231.0 30 89033.0 31 108797.0 32 134155.0 33 169389.0 34 220018.0 35 297250.0 36 408538.0 37 486977.0 38 256320.0 39 12398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.63654669220147 20.177240846780485 15.709695711090262 22.476516749927782 2 15.93585459410878 21.001339835450654 39.16140241273644 23.901403157704127 3 19.74337707129599 24.184638397478615 30.19357727214223 25.878407259083165 4 13.570587189648927 17.315010071819337 36.269023420760114 32.84537931777162 5 13.630021410669926 37.52259554119571 34.198612086977846 14.648770961156506 6 32.246774603387685 36.63415485075384 16.60721062338675 14.511859922471729 7 28.422582552192914 33.01811119712251 20.163951898690293 18.395354351994282 8 26.43525216603043 35.595957554543986 19.10126655570828 18.8675237237173 9 25.941950790089862 16.96320929129601 20.30461064927622 36.79022926933791 10-11 25.116438080830466 27.073010890173695 26.568203082617288 21.242347946378555 12-13 25.784583882175543 24.13777142679058 27.65808822376866 22.419556467265217 14-15 22.620187812992455 25.581876927649994 26.12918846096683 25.668746798390725 16-17 21.804153157980725 28.98164023445092 26.89309381761642 22.321112789951936 18-19 21.79790206772789 27.31101331463784 29.48336859636655 21.40771602126772 20-21 22.42687775606385 26.14586057050637 30.002570513007278 21.42469116042251 22-23 22.186870501076534 26.04286143149761 29.942241917888445 21.82802614953741 24-25 22.165056811162124 26.25520601491337 29.96466566624636 21.615071507678145 26-27 21.767132805752105 26.481692380658046 29.936706479896323 21.81446833369353 28-29 21.734918075259568 26.78997559453177 29.847583379203073 21.627522951005584 30-31 21.87065108897147 26.405698044994214 29.800427913944418 21.9232229520899 32-33 21.784942909464466 26.3606496193196 29.782799467384883 22.071608003831052 34-35 22.27562181752201 26.452198336951838 29.645509707260892 21.626670138265265 36-37 22.309053452715418 26.22420356420278 29.66259121274504 21.804151770336762 38-39 21.877068138555902 26.691717746442823 29.582985819980003 21.848228295021276 40-41 22.228694595973593 26.54060474098611 29.494349027641093 21.736351635399203 42-43 22.03503523627021 26.477461144196667 29.311338867607162 22.17616475192596 44-45 22.107414014317396 26.666099950323197 29.281526608438714 21.9449594269207 46-47 22.46813326955623 26.987081448940025 28.66925167995746 21.875533601546287 48-49 22.353032073809334 27.093847857683144 28.494866545677027 22.058253522830494 50-51 22.420632359552386 27.24155060967492 28.3909214254551 21.946895605317593 52-53 22.618550842117898 27.228205219458484 28.146230545782974 22.00701339264064 54-55 22.503058239877383 27.23919031601013 28.17742548755738 22.080325956555107 56-57 22.449804114065618 27.451967101931864 28.116426648158033 21.98180213584449 58-59 22.5668046086209 27.11682384132631 28.176614136610112 22.139757413442684 60-61 22.325335502836595 27.272308186494175 28.387805100225815 22.014551210443415 62-63 22.23953514016272 27.263696802576554 28.54670080733107 21.950067249929653 64-65 22.214678297505426 27.299064327959854 28.367816363252146 22.11844101128257 66-67 22.1931590576085 27.35040071516898 28.160442437047468 22.295997790175058 68-69 22.29570275346696 27.22917750772904 28.322048793908376 22.153070944895624 70-71 22.41596709160558 27.190029824523233 28.10243084431761 22.29157223955358 72-73 22.37954849794968 27.28967847268347 28.001509850353695 22.329263179013154 74-75 22.19771368678977 27.755154199091507 27.759026555885303 22.288105558233422 76-77 22.356332796981185 27.402308736000048 27.95395362096708 22.28740484605169 78-79 22.377944235849395 27.498140346749267 27.85721846029931 22.26669695710203 80-81 22.39887340233013 27.472509032733218 27.813350189763923 22.315267375172734 82-83 22.44978831061545 27.504812798725435 27.82171992092996 22.223678969729153 84-85 22.34808238481825 27.5946987647883 27.770393642825297 22.286825207568157 86-87 22.433374257383388 27.58714923362371 27.725503009927614 22.25397349906529 88-89 22.387957044130484 27.578519409911834 27.623457188514074 22.41006635744361 90-91 22.351369937664973 27.509440803116952 27.680820171903115 22.45836908731496 92-93 22.37837030453179 27.365909294171125 27.777772655481414 22.477947745815673 94-95 22.354415058378542 27.245681123588295 27.833025450235237 22.566878367797923 96-97 22.340437694332383 27.23738321617303 27.801604040822752 22.62057504867184 98-99 22.170299629542896 27.284341041816106 27.595569266627695 22.949790062013303 100-101 22.275286633577636 27.029524701600288 28.025552063214025 22.66963660160805 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 833.0 1 582.0 2 432.5 3 550.5 4 468.5 5 482.5 6 991.0 7 2122.5 8 3363.5 9 4004.5 10 3614.0 11 2995.5 12 2980.0 13 3229.0 14 3529.0 15 3738.5 16 3964.5 17 4260.5 18 4585.0 19 5002.5 20 5551.0 21 6562.5 22 8014.0 23 9581.0 24 11318.5 25 13824.0 26 17567.0 27 21689.0 28 24540.0 29 27494.5 30 32006.0 31 37467.0 32 43664.0 33 48269.0 34 52248.5 35 57088.0 36 63420.5 37 69574.0 38 73695.5 39 76661.5 40 79469.5 41 83900.5 42 87117.0 43 90237.0 44 93809.0 45 96913.0 46 99608.5 47 103303.0 48 107068.0 49 105415.0 50 100092.0 51 95113.0 52 92561.5 53 91925.5 54 91256.5 55 84791.0 56 75516.0 57 67535.0 58 61012.0 59 54352.5 60 44959.5 61 37233.5 62 30256.5 63 22519.5 64 16301.0 65 12188.0 66 9056.5 67 6997.5 68 5506.0 69 4278.0 70 3207.0 71 2051.0 72 1362.0 73 1011.5 74 695.5 75 481.5 76 329.0 77 196.0 78 124.5 79 87.5 80 57.0 81 38.5 82 16.5 83 9.0 84 8.0 85 3.5 86 1.5 87 2.0 88 1.0 89 1.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036068237565032545 2 0.0 3 0.0 4 3.687958851230321E-5 5 0.009662452190223443 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005070943420441692 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.2127753107381928E-4 22-23 0.0016595814830536449 24-25 0.0 26-27 0.0012170264209060062 28-29 0.0016411416887974928 30-31 1.8439794256151606E-5 32-33 1.8439794256151606E-5 34-35 0.0 36-37 3.3191629661072895E-4 38-39 1.8439794256151606E-5 40-41 9.219897128075804E-5 42-43 0.019988736973668344 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.8439794256151606E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.5815711958612254E-4 84-85 2.950367080984257E-4 86-87 0.0 88-89 0.0 90-91 0.010713520462824084 92-93 0.002563131401605073 94-95 0.0 96-97 0.0 98-99 4.4255506214763856E-4 100-101 0.013147573304636095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2711527.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.997668849484675 #Duplication Level Percentage of deduplicated Percentage of total 1 73.35557327966852 37.40963234380642 2 14.415189970597241 14.70282169045862 3 5.0281825529653785 7.6927676625265455 4 2.3335208803576477 4.760165004393491 5 1.2852933338012247 3.2773481905822512 6 0.768798407784009 2.3524155967307987 7 0.507798730122242 1.8127586036674057 8 0.37216969860378174 1.5183829636166546 9 0.2751929259295275 1.2630777935647328 >10 1.495761040653999 14.077053718375604 >50 0.11044988115488283 3.852846832190162 >100 0.04795107768059599 4.430025976970782 >500 0.002962228910879596 1.0090154806134861 >1k 9.392433131929004E-4 0.7293577300905767 >5k 7.22494856302231E-5 0.2652511391221047 >10k+ 1.444989712604462E-4 0.8470792732903439 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12197 0.4498203410845623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10793 0.3980413988132886 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7199 0.26549615770007085 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2747 0.10130822964329693 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.687958851230322E-5 2 0.0 0.0 0.0 0.0 3.687958851230322E-5 3 0.0 0.0 0.0 0.0 3.687958851230322E-5 4 0.0 0.0 0.0 0.0 3.687958851230322E-5 5 0.0 0.0 0.0 0.0 3.687958851230322E-5 6 0.0 0.0 0.0 0.0 7.375917702460644E-5 7 0.0 0.0 0.0 0.0 7.375917702460644E-5 8 0.0 0.0 0.0 0.0 7.375917702460644E-5 9 0.0 0.0 0.0 0.0 7.375917702460644E-5 10-11 0.0 0.0 0.0 5.531938276845483E-5 1.4751835404921287E-4 12-13 0.0 0.0 0.0 7.375917702460644E-5 2.3971732532997088E-4 14-15 0.0 0.0 0.0 7.375917702460644E-5 2.9503670809842575E-4 16-17 0.0 0.0 0.0 1.1063876553690964E-4 3.3191629661072895E-4 18-19 0.0 0.0 0.0 1.2907855979306127E-4 3.3191629661072895E-4 20-21 0.0 0.0 0.0 2.2127753107381928E-4 3.3191629661072895E-4 22-23 0.0 0.0 0.0 3.6879588512303216E-4 3.5035609086688055E-4 24-25 0.0 0.0 0.0 4.4255506214763856E-4 3.6879588512303216E-4 26-27 0.0 0.0 0.0 5.16314239172245E-4 3.6879588512303216E-4 28-29 0.0 0.0 0.0 0.0012170264209060062 3.6879588512303216E-4 30-31 0.0 0.0 0.0 0.0030794456407773185 3.6879588512303216E-4 32-33 0.0 0.0 0.0 0.006048252516017727 3.6879588512303216E-4 34-35 0.0 0.0 0.0 0.009348975687868865 3.6879588512303216E-4 36-37 0.0 0.0 0.0 0.014198641577236739 3.6879588512303216E-4 38-39 0.0 0.0 0.0 0.020431292035815982 3.6879588512303216E-4 40-41 0.0 0.0 0.0 0.029116435130463386 3.6879588512303216E-4 42-43 0.0 0.0 0.0 0.03986683518179977 3.6879588512303216E-4 44-45 0.0 0.0 0.0 0.05205553918511599 3.8723567937918376E-4 46-47 0.0 3.687958851230322E-5 0.0 0.066530777676195 4.0567547363533536E-4 48-49 0.0 3.687958851230322E-5 0.0 0.08272091703309611 4.2411526789148696E-4 50-51 0.0 3.687958851230322E-5 0.0 0.10298625092060673 4.794346506599418E-4 52-53 0.0 3.687958851230322E-5 0.0 0.13025870662545497 4.794346506599418E-4 54-55 0.0 3.687958851230322E-5 0.0 0.16337657710950323 4.794346506599418E-4 56-57 0.0 3.687958851230322E-5 0.0 0.19614409150268464 4.794346506599418E-4 58-59 0.0 3.687958851230322E-5 0.0 0.23962512635869015 4.794346506599418E-4 60-61 0.0 3.687958851230322E-5 0.0 0.29072179624248623 4.794346506599418E-4 62-63 0.0 3.687958851230322E-5 0.0 0.33726383694501294 5.531938276845482E-4 64-65 0.0 3.687958851230322E-5 0.0 0.38419311332691874 5.531938276845482E-4 66-67 0.0 3.687958851230322E-5 0.0 0.4541352529405018 5.531938276845482E-4 68-69 0.0 3.687958851230322E-5 0.0 0.5306419593092748 5.531938276845482E-4 70-71 0.0 3.687958851230322E-5 0.0 0.6012848111045916 5.531938276845482E-4 72-73 0.0 3.687958851230322E-5 0.0 0.6837660108123578 5.900734161968515E-4 74-75 0.0 3.687958851230322E-5 0.0 0.7799848572409569 6.269530047091547E-4 76-77 0.0 3.687958851230322E-5 0.0 0.8871016220749415 6.453927989653063E-4 78-79 0.0 3.687958851230322E-5 0.0 1.0091914998449214 6.822723874776095E-4 80-81 0.0 3.687958851230322E-5 0.0 1.145977893637054 7.007121817337611E-4 82-83 0.0 3.687958851230322E-5 0.0 1.296575693327044 7.375917702460643E-4 84-85 0.0 3.687958851230322E-5 0.0 1.448703995940295 7.375917702460643E-4 86-87 0.0 3.687958851230322E-5 0.0 1.6189586163073426 7.375917702460643E-4 88-89 0.0 3.687958851230322E-5 0.0 1.8143835558340373 7.375917702460643E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9280 0.0 35.797516 1 GTATCAA 13440 0.0 31.824886 1 ATCAACG 16670 0.0 25.390163 3 TATCAAC 16785 0.0 25.329407 2 TCAACGC 16735 0.0 25.291546 4 CAACGCA 16960 0.0 24.928005 5 AACGCAG 17575 0.0 24.052599 6 GTACATG 9900 0.0 23.426548 1 TACATGG 9665 0.0 23.395292 2 GAGTACT 12020 0.0 21.477238 12-13 CGCAGAG 19940 0.0 20.986214 8 ACGCAGA 20050 0.0 20.918457 7 ACATGGG 9970 0.0 20.91668 3 GTACTTT 12330 0.0 20.80166 14-15 AGTACTT 12390 0.0 20.548344 12-13 AGAGTAC 16845 0.0 20.344576 10-11 GTGTAAG 1060 0.0 19.279078 1 GGACCGA 740 0.0 19.25559 6 GCAGAGT 21320 0.0 19.070843 9 CATGGGG 5965 0.0 18.794247 4 >>END_MODULE