FastQCFastQC Report
Thu 26 May 2016
SRR937860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508313
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105430.42032234414126146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84320.3361621934742594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54650.2178755203198325No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35360.14097124242468942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74200.036.8559461
GTATCAA113050.035.2967721
TATCAAC138050.028.6498262
ATCAACG138650.028.4212843
TCAACGC139300.028.220984
CAACGCA140100.028.0943245
AACGCAG145100.027.0439326
GTACATG89950.023.9519371
TACATGG86900.023.9054412
ACGCAGA164400.023.695217
CGCAGAG165050.023.5731128
GAGTACT94950.022.76235612-13
ACATGGG90700.021.697123
GCAGAGT177400.021.288489
AGAGTAC138750.021.20888110-11
GTACTTT102500.020.9452214-15
AGTACTT99400.020.88314212-13
GTATAGG5850.020.3248671
GGACCGA6450.019.8866336
CAGAGTA168750.018.55030610-11
Produced by FastQC (version 0.11.5)