##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937860_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2508313 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59426714289644 31.0 31.0 34.0 30.0 34.0 2 31.69030420047259 33.0 31.0 34.0 30.0 34.0 3 31.73407624965465 33.0 31.0 34.0 30.0 34.0 4 35.37466097731822 37.0 35.0 37.0 33.0 37.0 5 35.37255238879677 37.0 35.0 37.0 33.0 37.0 6 35.431011600226924 37.0 35.0 37.0 33.0 37.0 7 35.34444465264104 37.0 35.0 37.0 33.0 37.0 8 35.34114442655282 37.0 35.0 37.0 33.0 37.0 9 36.887594969208386 39.0 37.0 39.0 33.0 39.0 10-11 36.7920584472512 39.0 37.0 39.0 33.0 39.0 12-13 36.674414237776546 39.0 37.0 39.0 32.0 39.0 14-15 37.74527560954314 40.0 37.5 41.0 32.5 41.0 16-17 37.63587957324305 40.0 37.0 41.0 32.0 41.0 18-19 37.54340985355496 40.0 37.0 41.0 32.0 41.0 20-21 37.54978824413062 40.0 37.0 41.0 32.0 41.0 22-23 37.55549427045189 40.0 37.0 41.0 32.0 41.0 24-25 37.466776873540105 40.0 37.0 41.0 32.0 41.0 26-27 37.349017247847456 40.0 37.0 41.0 32.0 41.0 28-29 37.18995256971519 39.5 37.0 41.0 31.0 41.0 30-31 36.93002069518437 39.0 36.0 41.0 31.0 41.0 32-33 36.77078658046264 39.0 36.0 41.0 30.0 41.0 34-35 36.72500740537565 39.0 36.0 41.0 30.0 41.0 36-37 36.5294935281203 39.0 35.5 41.0 30.0 41.0 38-39 36.33350742112329 39.0 35.0 41.0 30.0 41.0 40-41 36.07620918920406 39.0 35.0 40.5 29.5 41.0 42-43 35.783072726569614 39.0 35.0 40.0 28.5 41.0 44-45 35.50764956367088 38.5 35.0 40.0 27.0 41.0 46-47 35.1214517486454 38.0 34.5 40.0 25.5 41.0 48-49 34.72387497094661 38.0 34.0 40.0 24.0 41.0 50-51 33.83975086043887 37.5 33.0 39.5 23.5 40.5 52-53 33.956872009195024 38.0 33.5 39.5 23.0 40.5 54-55 34.18965316529476 38.0 34.0 40.0 23.0 41.0 56-57 33.89880170457196 38.0 33.0 40.0 22.0 41.0 58-59 33.571894536287935 37.5 33.0 40.0 20.5 41.0 60-61 33.174941883249815 37.0 32.5 40.0 19.0 41.0 62-63 32.753077068133045 36.5 32.0 39.5 17.5 41.0 64-65 32.27406747084594 36.0 31.0 39.0 13.5 40.5 66-67 31.73838791251331 35.5 31.0 39.0 8.0 40.0 68-69 31.211191545871667 35.0 30.0 38.0 7.5 40.0 70-71 30.695570489009945 35.0 29.5 37.5 4.0 40.0 72-73 30.167899101906343 34.5 29.0 37.0 2.0 39.0 74-75 29.578188009231702 34.0 29.0 36.5 2.0 39.0 76-77 28.990799393855553 34.0 28.0 36.0 2.0 38.5 78-79 28.4030585497105 34.0 26.5 35.0 2.0 37.5 80-81 27.79823610530265 33.0 26.0 35.0 2.0 37.0 82-83 27.18598336810438 33.0 25.5 35.0 2.0 36.5 84-85 26.567730781605007 32.0 24.5 35.0 2.0 36.0 86-87 25.921624215159753 32.0 22.5 35.0 2.0 35.5 88-89 25.280458419662935 31.5 20.0 34.0 2.0 35.0 90-91 24.618454515046565 31.0 17.5 34.0 2.0 35.0 92-93 23.845866125957965 31.0 8.5 34.0 2.0 35.0 94-95 22.957696268368423 30.5 2.0 34.0 2.0 35.0 96-97 21.943550705195086 29.5 2.0 34.0 2.0 35.0 98-99 20.64692105809761 29.0 2.0 34.0 2.0 35.0 100-101 17.532756876833155 22.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10926462930256164 1101 2 0.07205225925321557 1101 3 0.02368175023115171 1101 4 0.08912964502008691 1101 5 -0.010910056193530693 1101 6 0.04200934003662837 1101 7 -0.03158967617896735 1101 8 0.038342946534953626 1101 9 0.28430371694545187 1101 10-11 0.2675109706611778 1101 12-13 0.3370554792728768 1101 14-15 0.5225615310599636 1101 16-17 0.5105021683188156 1101 18-19 0.5519676412407577 1101 20-21 0.5375612533753582 1101 22-23 0.5254425245554728 1101 24-25 0.49436697463092116 1101 26-27 0.5458559562707279 1101 28-29 0.5171814036647433 1101 30-31 0.5233485795275286 1101 32-33 0.5030257991713185 1101 34-35 0.621838933560781 1101 36-37 0.7065377416982628 1101 38-39 0.6782984089389288 1101 40-41 0.7347610906977238 1101 42-43 0.6743228960513434 1101 44-45 0.7790252418568642 1101 46-47 0.7389720295863142 1101 48-49 0.8234534414182306 1101 50-51 0.7584127073633908 1101 52-53 0.777837350532657 1101 54-55 0.7675124682231598 1101 56-57 1.0336626189059785 1101 58-59 1.0355360280890338 1101 60-61 1.0060400429204819 1101 62-63 1.0022203223343027 1101 64-65 0.9648876450351906 1101 66-67 1.018921769257883 1101 68-69 1.2376560509609362 1101 70-71 1.2433073783002726 1101 72-73 1.1214932874401633 1101 74-75 1.250722235206073 1101 76-77 1.2283125776394215 1101 78-79 1.4618460663140986 1101 80-81 1.6856464758244165 1101 82-83 1.840341267197946 1101 84-85 2.0420401611792087 1101 86-87 2.3058567311645426 1101 88-89 2.3737304783314244 1101 90-91 2.4873484355971556 1101 92-93 2.47823301707432 1101 94-95 2.819606788135026 1101 96-97 3.102262514981245 1101 98-99 3.2486429085003046 1101 100-101 3.761361648728922 1102 1 0.19493522865884572 1102 2 0.14672532713642283 1102 3 0.08108501916701272 1102 4 0.05865977723140503 1102 5 0.09016687399673629 1102 6 0.004845808476218849 1102 7 0.1432734759834986 1102 8 0.034053720460526904 1102 9 0.24044549413071792 1102 10-11 0.3596299223206927 1102 12-13 0.21981143160795114 1102 14-15 0.2998062594320956 1102 16-17 0.39661914464304715 1102 18-19 0.29011081323363896 1102 20-21 0.37612877567815417 1102 22-23 0.17999324494920899 1102 24-25 0.21543075304402493 1102 26-27 0.2746318263558294 1102 28-29 0.3458052705440977 1102 30-31 0.4619162944833093 1102 32-33 0.4182615036776056 1102 34-35 0.46598209014142355 1102 36-37 0.5009599240199094 1102 38-39 0.5379668916810729 1102 40-41 0.525698437378054 1102 42-43 0.5758854402009916 1102 44-45 0.6697125891793689 1102 46-47 0.6624288791705695 1102 48-49 0.7053736651011988 1102 50-51 0.5672195817356567 1102 52-53 0.6935005520142923 1102 54-55 0.7015476363835234 1102 56-57 0.8192685510767177 1102 58-59 0.8385243969861094 1102 60-61 0.7922714551182395 1102 62-63 0.8799129688982745 1102 64-65 0.8610253379771606 1102 66-67 0.9098160258870784 1102 68-69 0.8525604565494156 1102 70-71 0.9609494470337197 1102 72-73 0.8667246765195635 1102 74-75 0.7619618030468978 1102 76-77 0.8487676368352624 1102 78-79 1.1683089960265818 1102 80-81 1.2504327209149757 1102 82-83 1.436543755760777 1102 84-85 1.6277944361489638 1102 86-87 1.5646665595492628 1102 88-89 1.7734759195196084 1102 90-91 1.944927211403023 1102 92-93 2.033686830447518 1102 94-95 2.2125545971568386 1102 96-97 2.3145543534301574 1102 98-99 2.8184302966652446 1102 100-101 3.25058226465336 1103 1 0.04069153606496201 1103 2 -0.0325910314265343 1103 3 0.016540198797287076 1103 4 0.04330691128558328 1103 5 -0.044974388647140984 1103 6 0.036097988455289 1103 7 0.012660756532277162 1103 8 -0.04107707828380569 1103 9 -0.011961202764993573 1103 10-11 0.06629193836533176 1103 12-13 0.1641463926589921 1103 14-15 0.13440107398854195 1103 16-17 0.2088822535838588 1103 18-19 0.15386212122108134 1103 20-21 0.1853747935830441 1103 22-23 0.0449798744108989 1103 24-25 0.08627716625179005 1103 26-27 0.09689958591866343 1103 28-29 0.10721673432737333 1103 30-31 0.059887460626775635 1103 32-33 0.1598409711813744 1103 34-35 0.1377906007797236 1103 36-37 0.2153710390065413 1103 38-39 0.2470356046214306 1103 40-41 0.3514512568176542 1103 42-43 0.2837449914159649 1103 44-45 0.3687621183038914 1103 46-47 0.40842643759400943 1103 48-49 0.4276821176067145 1103 50-51 0.29918970148917623 1103 52-53 0.2859781708958167 1103 54-55 0.3034166051080973 1103 56-57 0.36993998463095323 1103 58-59 0.5155764839527421 1103 60-61 0.5591386798595508 1103 62-63 0.6856187007899166 1103 64-65 0.5250992828672878 1103 66-67 0.8256949934489874 1103 68-69 0.8991945688619367 1103 70-71 0.9992269729028038 1103 72-73 0.9038918663812048 1103 74-75 0.9353050189520253 1103 76-77 0.9837199679595514 1103 78-79 1.1203232966892998 1103 80-81 1.3116058403196966 1103 82-83 1.4777337336703233 1103 84-85 1.680262172458697 1103 86-87 1.625191498509853 1103 88-89 1.7489671414705406 1103 90-91 1.9527315364815045 1103 92-93 1.8653662863247398 1103 94-95 1.9216029636258511 1103 96-97 2.051221601424576 1103 98-99 2.4233832091172864 1103 100-101 2.880616562990763 1104 1 0.12325519630500636 1104 2 0.021761577247392694 1104 3 0.11128506059571208 1104 4 0.05558270998868409 1104 5 0.062156635189886345 1104 6 0.11391869339321659 1104 7 0.10649712190623006 1104 8 0.06891744963520807 1104 9 0.311122853347122 1104 10-11 0.1472708538745593 1104 12-13 0.0568920893378575 1104 14-15 0.17464991541607588 1104 16-17 0.24349547011478734 1104 18-19 0.15449840829770523 1104 20-21 0.2048807854632173 1104 22-23 0.14553221258550764 1104 24-25 0.09958304296600318 1104 26-27 0.08060298409066036 1104 28-29 0.10810170274405806 1104 30-31 0.2470379666244824 1104 32-33 0.1913843890877942 1104 34-35 0.31113271305149226 1104 36-37 0.26499118295913604 1104 38-39 0.3213495448932804 1104 40-41 0.4672637516936504 1104 42-43 0.41912047901573857 1104 44-45 0.4383355288414279 1104 46-47 0.39567540951709645 1104 48-49 0.5454556840597533 1104 50-51 0.38922981067854323 1104 52-53 0.5914118551441305 1104 54-55 0.6503629728930633 1104 56-57 0.5826507604100044 1104 58-59 0.6746041077743357 1104 60-61 0.713860738894958 1104 62-63 0.8493050520713226 1104 64-65 0.8875907509325032 1104 66-67 1.024567601657207 1104 68-69 0.9686029801489795 1104 70-71 1.0249681294038346 1104 72-73 1.062813856724535 1104 74-75 1.0436961859072547 1104 76-77 1.1244228514260541 1104 78-79 1.256935338760286 1104 80-81 1.3276674046265917 1104 82-83 1.2865469615529044 1104 84-85 1.571616381522265 1104 86-87 1.7687909344198012 1104 88-89 1.8838136127302327 1104 90-91 1.9376254033362628 1104 92-93 2.101728288737693 1104 94-95 2.402021597225886 1104 96-97 2.606925554566484 1104 98-99 2.8128625755395724 1104 100-101 3.243803987100808 1105 1 0.1191821484976181 1105 2 0.056129075514956384 1105 3 0.11338980975580881 1105 4 0.11735629375419165 1105 5 0.05915179996849673 1105 6 0.05048090069468003 1105 7 -0.03796425431415429 1105 8 0.08850808835030932 1105 9 0.24602109537719485 1105 10-11 0.20223604437409648 1105 12-13 0.18824605366384617 1105 14-15 0.3026513393746413 1105 16-17 0.24839331253978258 1105 18-19 0.22776270904243034 1105 20-21 0.23609152548308288 1105 22-23 0.22986849779826457 1105 24-25 0.18923285734457806 1105 26-27 0.25810792311190056 1105 28-29 0.18116412982831065 1105 30-31 0.06256661977020173 1105 32-33 0.11462780803083206 1105 34-35 0.40611128544775 1105 36-37 0.3105319071020105 1105 38-39 0.21581433237647474 1105 40-41 0.11141787059857222 1105 42-43 0.15470899007748784 1105 44-45 0.2703734757290377 1105 46-47 0.28860378859221214 1105 48-49 0.2681275702834185 1105 50-51 0.24217733668454144 1105 52-53 0.35720161609257417 1105 54-55 0.4321197586257952 1105 56-57 0.5558260515612901 1105 58-59 0.40404783801946564 1105 60-61 0.4230821376491676 1105 62-63 0.42499402969635725 1105 64-65 0.4859794896716778 1105 66-67 0.7036071216242981 1105 68-69 0.6837253762589697 1105 70-71 0.5885398141680653 1105 72-73 0.6612197736086323 1105 74-75 0.7758590581125766 1105 76-77 0.8436787234276792 1105 78-79 1.1165410406373582 1105 80-81 1.3460210950769067 1105 82-83 1.066735175542398 1105 84-85 1.23278295543507 1105 86-87 1.4389851717247844 1105 88-89 1.4636117000838986 1105 90-91 1.6750428385929332 1105 92-93 1.7212954872039923 1105 94-95 1.80374140974288 1105 96-97 1.943666536405896 1105 98-99 2.055776164353748 1105 100-101 2.573626318786669 1106 1 -0.29867578227996816 1106 2 -0.3036533241437809 1106 3 -0.1500545408762619 1106 4 -0.12597178906261775 1106 5 -0.20177326303896592 1106 6 -0.15007665816640525 1106 7 -0.24422502143205094 1106 8 -0.2734111190634536 1106 9 -0.19567062559521275 1106 10-11 -0.4890898757319633 1106 12-13 -0.5230332612637909 1106 14-15 -0.6534152816185141 1106 16-17 -0.4826662261948016 1106 18-19 -0.18586286148688203 1106 20-21 -0.1004699741715882 1106 22-23 -0.32610724789849144 1106 24-25 -0.4889387625227499 1106 26-27 -0.4498332535816658 1106 28-29 -0.25443514296714653 1106 30-31 -0.16660275828746052 1106 32-33 -0.08015477231710832 1106 34-35 -0.14493568843772664 1106 36-37 -0.486316190897746 1106 38-39 -0.5546764548750005 1106 40-41 -0.2979342107767735 1106 42-43 -0.3160847485236147 1106 44-45 -0.33792354497136756 1106 46-47 -0.23128731267940594 1106 48-49 -0.04863222502983433 1106 50-51 0.15825516293532615 1106 52-53 0.031894589759211556 1106 54-55 0.049344847586795026 1106 56-57 -0.1725363533589217 1106 58-59 -0.35570868391303634 1106 60-61 -0.1543958125822087 1106 62-63 -0.11610518232182443 1106 64-65 -0.0244416652684194 1106 66-67 0.17854123324372395 1106 68-69 0.021979857919774304 1106 70-71 -0.0961157050278274 1106 72-73 0.013295778815511028 1106 74-75 0.15793187156474886 1106 76-77 0.487639496433772 1106 78-79 0.6328149462609858 1106 80-81 0.6824978729765228 1106 82-83 0.4538204717122163 1106 84-85 0.5939231775842302 1106 86-87 0.7619168300144636 1106 88-89 1.0072540178487337 1106 90-91 1.0160144815076002 1106 92-93 1.0780701697911859 1106 94-95 1.184663624684184 1106 96-97 1.4537447609310554 1106 98-99 1.6392380860630134 1106 100-101 2.2149499771188026 1107 1 0.08154012456367354 1107 2 0.16450157971020474 1107 3 0.12772194435575557 1107 4 0.11561470745332514 1107 5 0.13618609156708317 1107 6 0.06531252089900619 1107 7 0.12167658739965503 1107 8 0.2139439057728154 1107 9 0.12846882975185991 1107 10-11 0.1659099497743597 1107 12-13 0.20173204473995554 1107 14-15 0.22999385952360996 1107 16-17 0.22847112359522725 1107 18-19 0.33549516464064055 1107 20-21 0.2226341520222448 1107 22-23 0.27001841081514044 1107 24-25 0.279924004016344 1107 26-27 0.28488691672416167 1107 28-29 0.2872717541862855 1107 30-31 0.18026449855560145 1107 32-33 0.2810674977293033 1107 34-35 0.30954763578314726 1107 36-37 0.37293430059277455 1107 38-39 0.5514535080160101 1107 40-41 0.6507384739829547 1107 42-43 0.5993468906699917 1107 44-45 0.6881923420963219 1107 46-47 0.6986505675713985 1107 48-49 0.5930595493382143 1107 50-51 0.39859112142909225 1107 52-53 0.5618144389966702 1107 54-55 0.717545119796938 1107 56-57 0.8158540006305941 1107 58-59 0.6750778172560672 1107 60-61 0.7894288561706304 1107 62-63 0.8258349547138764 1107 64-65 0.8570102384576046 1107 66-67 0.88841932032771 1107 68-69 0.9743518086427585 1107 70-71 1.0820830549037694 1107 72-73 1.1838546239000252 1107 74-75 1.2490757357854 1107 76-77 1.4670986823626038 1107 78-79 1.5229246446195823 1107 80-81 1.6492888179179488 1107 82-83 1.8992010424228702 1107 84-85 2.1629179776726417 1107 86-87 2.194230732507396 1107 88-89 2.0485159561245005 1107 90-91 1.940380196989846 1107 92-93 2.17931140148389 1107 94-95 2.389007353963059 1107 96-97 2.3131988283786775 1107 98-99 2.571107029678288 1107 100-101 2.9264973870509827 1108 1 -0.06945930772150533 1108 2 -0.12207483630519178 1108 3 -0.06523754453143837 1108 4 -0.04795377091090813 1108 5 0.017185815712288388 1108 6 0.04885538148500501 1108 7 0.04797534096905309 1108 8 0.03798361568277642 1108 9 0.1829899298193638 1108 10-11 0.17709336685268795 1108 12-13 0.2018233686829376 1108 14-15 0.20807611321022534 1108 16-17 0.14901458578946603 1108 18-19 0.279516294364889 1108 20-21 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-0.9464062201892425 2316 68-69 -1.0590100341795008 2316 70-71 -1.0589875216634326 2316 72-73 -1.2097677736599088 2316 74-75 -1.3127783128839958 2316 76-77 -1.088499832835513 2316 78-79 -1.3231117223833806 2316 80-81 -1.5137302075641763 2316 82-83 -1.5992116782644494 2316 84-85 -1.70527213936996 2316 86-87 -1.9422308201413614 2316 88-89 -1.8607783073629527 2316 90-91 -1.83633938445465 2316 92-93 -2.2239229705716816 2316 94-95 -1.8335481775764038 2316 96-97 -2.1271460228367793 2316 98-99 -2.469569838926443 2316 100-101 -2.6819372360679132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10465.0 3 2208.0 4 2278.0 5 2458.0 6 2940.0 7 3446.0 8 4459.0 9 5752.0 10 7095.0 11 8758.0 12 11379.0 13 14182.0 14 16510.0 15 19933.0 16 23754.0 17 28892.0 18 26330.0 19 21318.0 20 22165.0 21 23948.0 22 26191.0 23 29305.0 24 32724.0 25 37236.0 26 42991.0 27 49895.0 28 57980.0 29 68961.0 30 81847.0 31 99626.0 32 122806.0 33 154922.0 34 201463.0 35 266600.0 36 348743.0 37 396287.0 38 217635.0 39 14830.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41181030954889 18.803871831295236 14.81563117674661 22.968686682409263 2 15.273092637631835 19.77533184252918 40.73193872642997 24.21963679340901 3 19.4345805135997 23.80323673767231 29.73376383210548 27.028418916622517 4 12.759977178342774 16.360945219626466 36.97108470441196 33.9079928976188 5 13.779834978787614 37.1316519353429 34.23068665280894 14.857826433060536 6 32.23958331252479 35.82710841760958 17.246449131215243 14.686859138650386 7 28.58804142655974 32.75766261522636 20.345913771235107 18.308382186978797 8 26.36193116783757 34.97919534007578 19.5486697202807 19.11020377180594 9 26.10659190906356 17.022016977071324 20.455744913250896 36.41564620061422 10-11 25.028066275563283 26.655111218360016 26.92510679167912 21.39171571439758 12-13 25.718112653857027 23.93690201393765 27.79551585989634 22.54946947230898 14-15 22.702042533419814 25.336315569425782 26.164347523325105 25.7972943738293 16-17 21.93193198791496 28.713825685775475 26.883393301777165 22.4708490245324 18-19 21.92477652959017 27.10247932068591 29.357685474697988 21.61505867502594 20-21 22.484953841938147 26.118780265576124 29.796645686395205 21.599620206090524 22-23 22.274479286408106 25.94360831144322 29.71834117089425 22.06357123125442 24-25 22.11063526865458 26.215690257520187 29.752530630319146 21.921143843506087 26-27 21.80088074681705 26.405225833695216 29.690048711539234 22.103844707948493 28-29 21.785422701620305 26.73978961255173 29.64727470965801 21.827512976169952 30-31 21.915211208856363 26.387482729881363 29.58274434471037 22.114561716551904 32-33 21.7344758666636 26.37733764696599 29.593624240511325 22.29456224585908 34-35 22.247166500460263 26.44929186483584 29.448058900992123 21.855482733711774 36-37 22.208657848003597 26.221118252603226 29.478671963430518 22.091551935962663 38-39 21.853267417970784 26.686754435316608 29.378411580364954 22.081566566347654 40-41 22.182336473645385 26.533732958578156 29.357237888722953 21.926692679053506 42-43 21.947294584215136 26.435735439920172 29.169472865950357 22.447497109914334 44-45 22.043471964926823 26.578937118946204 29.17553066656014 22.20206024956683 46-47 22.369665273888792 26.93906600121207 28.6000125441893 22.091256180709834 48-49 22.246811098235135 26.972136950228702 28.481123287687655 22.29992866384851 50-51 22.240759817684346 27.186622248350645 28.425864500165098 22.14675343379991 52-53 22.456503254863915 27.098320384734027 28.223478013486147 22.22169834691591 54-55 22.32673949650333 27.09373795241491 28.274895679149214 22.30462687193255 56-57 22.248954697640126 27.40080061633799 28.185461340886587 22.164783345135294 58-59 22.419998742142134 26.98214939957253 28.220700147648554 22.377151710636785 60-61 22.15010376112097 27.212152751706586 28.439573325324265 22.19817016184818 62-63 22.128553134753894 27.14303314917899 28.638179753459358 22.090233962607755 64-65 22.072556739619603 27.209935594051615 28.42041276360868 22.2970949027201 66-67 22.148922365633442 27.259565431587934 28.187437107957543 22.40407509482108 68-69 22.184597971445598 27.097028897874154 28.425561894233365 22.292811236446884 70-71 22.23284374906393 27.03994887821633 28.234720877058102 22.492486495661637 72-73 22.183890140440013 27.202463691703016 28.135375225678633 22.478270942178337 74-75 22.06005832068387 27.633418550770948 27.921147239753935 22.385375888791245 76-77 22.222035805242456 27.340240481898547 27.95987054938615 22.477853163472844 78-79 22.297252359105286 27.310876620296444 27.990701589034444 22.40116943156383 80-81 22.31219765107011 27.299948911993223 27.896408381309058 22.491445055627608 82-83 22.382289390968165 27.313374417387905 27.89313086855632 22.411205323087614 84-85 22.30013784388835 27.3490821090694 27.89029359769753 22.460486449344717 86-87 22.34759589808148 27.35383839949133 27.874026880698327 22.424538821728856 88-89 22.412248055110577 27.347962302308105 27.642484257844412 22.597305384736906 90-91 22.30363756843582 27.276091488215275 27.759451003605573 22.660819939743323 92-93 22.400786966486628 27.170093835688796 27.82773645489251 22.60138274293207 94-95 22.380411194139686 27.02862678539227 27.92074628662971 22.670215733838333 96-97 22.365958232077034 26.939906884150016 27.865473320263884 22.828661563509066 98-99 22.236959203172482 27.04681153955538 27.640513707056602 23.075715550215538 100-101 22.787457990530136 26.18749197549488 28.32529002533298 22.699760008642006 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3501.0 1 2054.5 2 622.0 3 641.0 4 552.0 5 538.5 6 944.0 7 1879.5 8 3026.0 9 3540.5 10 3106.0 11 2615.0 12 2682.0 13 2957.0 14 3137.0 15 3252.0 16 3461.0 17 3711.0 18 3916.0 19 4288.5 20 4855.5 21 5705.0 22 6936.0 23 8516.5 24 10261.5 25 12524.5 26 16125.0 27 20017.5 28 22489.5 29 25207.5 30 29363.0 31 34234.0 32 39953.5 33 44429.5 34 48269.0 35 53011.5 36 58765.0 37 64083.5 38 67508.5 39 70668.0 40 73537.0 41 77124.5 42 80236.0 43 82949.5 44 86402.5 45 89553.5 46 91913.5 47 95130.5 48 98131.0 49 96817.5 50 92183.5 51 87396.5 52 85248.5 53 84587.5 54 83485.0 55 77587.5 56 69690.0 57 63054.0 58 57418.0 59 51329.5 60 42827.5 61 35811.0 62 29009.5 63 21734.0 64 16198.5 65 12280.0 66 8923.5 67 6730.0 68 5290.0 69 4083.5 70 3089.0 71 2110.5 72 1471.5 73 1067.5 74 740.5 75 503.5 76 337.0 77 212.5 78 131.5 79 87.0 80 69.5 81 52.5 82 37.0 83 28.5 84 21.0 85 15.0 86 11.5 87 9.5 88 10.0 89 8.0 90 5.5 91 5.0 92 5.5 93 5.0 94 3.0 95 3.0 96 2.5 97 2.5 98 3.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33943132296487716 2 0.28712525111499243 3 0.2925472219774805 4 0.28624816759311933 5 0.28624816759311933 6 0.21265288662140647 7 0.2028454981495531 8 0.21002163605578728 9 0.21089871957766038 10-11 0.21165620080109618 12-13 0.21450672224718365 14-15 0.2100814372050059 16-17 0.20141027056830627 18-19 0.19556969166128788 20-21 0.19335704914019902 22-23 0.2029052992987717 24-25 0.20067272306127665 26-27 0.2077093249526674 28-29 0.21366950615812302 30-31 0.21667949733546016 32-33 0.21510473373936986 34-35 0.21532400461983814 36-37 0.20027404873315252 38-39 0.19134374378317218 40-41 0.18474568365271798 42-43 0.19642684146675476 44-45 0.19836041195815673 46-47 0.2057956881776716 48-49 0.21426751765030919 50-51 0.210958520726879 52-53 0.2231380214510709 54-55 0.2080282644151667 56-57 0.17908450819335545 58-59 0.16168237377073755 60-61 0.17880543616366856 62-63 0.17320406185352466 64-65 0.17968251968554164 66-67 0.18014099516288437 68-69 0.17784861777617067 70-71 0.1789250384621058 72-73 0.18948990815739503 74-75 0.19586869740738097 76-77 0.1979218701972202 78-79 0.1943338012441031 80-81 0.1909650031714543 82-83 0.179523049954292 84-85 0.17515756606133287 86-87 0.17976225455116646 88-89 0.18994838363473776 90-91 0.18697825989021305 92-93 0.18026059746132161 94-95 0.18165595760975603 96-97 0.18996831735114397 98-99 0.19076566600739223 100-101 0.17001466722853167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2508313.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.48984820997437 #Duplication Level Percentage of deduplicated Percentage of total 1 75.4271087939763 40.34584600307015 2 13.48320514949512 14.424291936608775 3 4.582875555157691 7.354119534317608 4 2.086114577400628 4.463438083750977 5 1.1640071078395786 3.1131281756835167 6 0.6982938083039036 2.2410977887284447 7 0.47490430313427573 1.7781791362241242 8 0.33676970380453697 1.4411008270578167 9 0.246523450731232 1.1867851763831425 >10 1.356167970983161 13.320826015850818 >50 0.09628213358449009 3.562975404545093 >100 0.04408613351264067 4.187000819039896 >500 0.0026152229117565088 0.9076325112479897 >1k 8.219272008339441E-4 0.6967615292847933 >5k 1.494413092425353E-4 0.5554348059697569 >10k+ 7.472065462126765E-5 0.4213822522371121 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10543 0.42032234414126146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8432 0.3361621934742594 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5465 0.2178755203198325 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3536 0.14097124242468942 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.986743281241217E-5 2 0.0 0.0 0.0 0.0 3.986743281241217E-5 3 0.0 0.0 0.0 0.0 3.986743281241217E-5 4 0.0 0.0 0.0 0.0 3.986743281241217E-5 5 0.0 0.0 0.0 0.0 3.986743281241217E-5 6 0.0 0.0 0.0 0.0 3.986743281241217E-5 7 0.0 0.0 0.0 0.0 3.986743281241217E-5 8 0.0 0.0 0.0 0.0 3.986743281241217E-5 9 0.0 0.0 0.0 0.0 3.986743281241217E-5 10-11 0.0 0.0 0.0 0.0 3.986743281241217E-5 12-13 0.0 0.0 0.0 0.0 3.986743281241217E-5 14-15 0.0 0.0 0.0 0.0 3.986743281241217E-5 16-17 0.0 0.0 0.0 1.196022984372365E-4 3.986743281241217E-5 18-19 0.0 0.0 0.0 1.196022984372365E-4 3.986743281241217E-5 20-21 0.0 0.0 0.0 1.196022984372365E-4 3.986743281241217E-5 22-23 0.0 0.0 0.0 2.192708804682669E-4 3.986743281241217E-5 24-25 0.0 0.0 0.0 3.787406117179156E-4 3.986743281241217E-5 26-27 0.0 0.0 0.0 6.378789249985947E-4 7.973486562482434E-5 28-29 0.0 0.0 0.0 0.0013156252828096016 7.973486562482434E-5 30-31 5.980114921861825E-5 0.0 0.0 0.0029501900281185003 7.973486562482434E-5 32-33 7.973486562482434E-5 0.0 0.0 0.006039916071080443 7.973486562482434E-5 34-35 7.973486562482434E-5 0.0 0.0 0.009647918740603743 1.196022984372365E-4 36-37 7.973486562482434E-5 0.0 0.0 0.013774198036688404 1.196022984372365E-4 38-39 7.973486562482434E-5 0.0 0.0 0.018917096869489572 1.5946973124964867E-4 40-41 7.973486562482434E-5 0.0 0.0 0.02625270450697341 1.5946973124964867E-4 42-43 7.973486562482434E-5 0.0 0.0 0.036239496426482665 1.5946973124964867E-4 44-45 7.973486562482434E-5 0.0 0.0 0.04738244389755186 1.5946973124964867E-4 46-47 1.196022984372365E-4 0.0 0.0 0.06067823274049132 1.5946973124964867E-4 48-49 1.196022984372365E-4 0.0 0.0 0.07564845376155208 1.9933716406206084E-4 50-51 1.196022984372365E-4 0.0 0.0 0.09550243530213334 2.39204596874473E-4 52-53 1.196022984372365E-4 0.0 0.0 0.12133653176457643 2.7907202968688517E-4 54-55 1.196022984372365E-4 0.0 0.0 0.15548298796840745 3.1893946249929734E-4 56-57 1.196022984372365E-4 0.0 0.0 0.18881216179958402 3.1893946249929734E-4 58-59 1.196022984372365E-4 0.0 0.0 0.23115137544636577 3.1893946249929734E-4 60-61 1.196022984372365E-4 0.0 0.0 0.281663412819692 3.1893946249929734E-4 62-63 1.196022984372365E-4 0.0 0.0 0.32685314791256115 3.588068953117095E-4 64-65 1.196022984372365E-4 0.0 0.0 0.37274056307964754 3.588068953117095E-4 66-67 1.196022984372365E-4 0.0 0.0 0.44201022759121367 3.588068953117095E-4 68-69 1.196022984372365E-4 0.0 0.0 0.5220241652457249 3.588068953117095E-4 70-71 1.196022984372365E-4 0.0 0.0 0.5930679305174434 3.588068953117095E-4 72-73 1.196022984372365E-4 0.0 0.0 0.676211461647729 4.385417609365338E-4 74-75 1.196022984372365E-4 0.0 0.0 0.7738268708889202 4.78409193748946E-4 76-77 1.196022984372365E-4 0.0 0.0 0.8817081440793075 4.78409193748946E-4 78-79 1.196022984372365E-4 0.0 0.0 1.010021476586056 4.78409193748946E-4 80-81 1.196022984372365E-4 0.0 0.0 1.1518299350998062 4.78409193748946E-4 82-83 1.196022984372365E-4 3.986743281241217E-5 0.0 1.307552127665088 5.382103429675642E-4 84-85 1.196022984372365E-4 3.986743281241217E-5 0.0 1.465706233631927 5.581440593737703E-4 86-87 1.196022984372365E-4 3.986743281241217E-5 0.0 1.6386710908885773 5.980114921861825E-4 88-89 1.196022984372365E-4 3.986743281241217E-5 0.0 1.837808917786576 6.378789249985947E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7420 0.0 36.855946 1 GTATCAA 11305 0.0 35.296772 1 TATCAAC 13805 0.0 28.649826 2 ATCAACG 13865 0.0 28.421284 3 TCAACGC 13930 0.0 28.22098 4 CAACGCA 14010 0.0 28.094324 5 AACGCAG 14510 0.0 27.043932 6 GTACATG 8995 0.0 23.951937 1 TACATGG 8690 0.0 23.905441 2 ACGCAGA 16440 0.0 23.69521 7 CGCAGAG 16505 0.0 23.573112 8 GAGTACT 9495 0.0 22.762356 12-13 ACATGGG 9070 0.0 21.69712 3 GCAGAGT 17740 0.0 21.28848 9 AGAGTAC 13875 0.0 21.208881 10-11 GTACTTT 10250 0.0 20.94522 14-15 AGTACTT 9940 0.0 20.883142 12-13 GTATAGG 585 0.0 20.324867 1 GGACCGA 645 0.0 19.886633 6 CAGAGTA 16875 0.0 18.550306 10-11 >>END_MODULE