FastQCFastQC Report
Thu 26 May 2016
SRR937860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508313
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111910.4461564406037045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98610.39313275496319644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68320.2723743009743999No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25490.10162208623883862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86350.037.858941
GTATCAA125400.032.5111351
ATCAACG156800.025.992843
TCAACGC157100.025.94324
TATCAAC158300.025.746542
CAACGCA159800.025.5048625
AACGCAG164100.024.9787926
GAGTACT110600.022.31177912-13
ACGCAGA186950.021.6734187
CGCAGAG187450.021.5649248
GTACATG96850.021.3418461
GTACTTT116200.021.01000614-15
TACATGG95800.020.8751242
AGAGTAC159050.020.56241210-11
AGTACTT114400.020.34575812-13
ACATGGG95300.020.0375963
GCAGAGT200500.019.6401129
TAAGGTG11050.019.344735
GTAAGGT9500.019.0008244
GGACCGA6400.018.5536426
Produced by FastQC (version 0.11.5)