##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937860_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2508313 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.828041795421864 31.0 31.0 34.0 30.0 34.0 2 32.07444924138255 33.0 31.0 34.0 30.0 34.0 3 32.17610680963659 34.0 31.0 34.0 30.0 34.0 4 35.89539901918142 37.0 35.0 37.0 35.0 37.0 5 35.67979235446294 37.0 35.0 37.0 33.0 37.0 6 35.78497619714924 37.0 35.0 37.0 35.0 37.0 7 35.73477711912349 37.0 35.0 37.0 33.0 37.0 8 35.72525478279625 37.0 35.0 37.0 33.0 37.0 9 37.331414779574956 39.0 37.0 39.0 34.0 39.0 10-11 37.25686706563336 39.0 37.0 39.0 33.5 39.0 12-13 37.15545488142827 39.0 37.0 39.0 33.0 39.0 14-15 38.26981461245067 40.0 38.0 41.0 33.0 41.0 16-17 38.22766576579558 40.0 38.0 41.0 33.0 41.0 18-19 38.19944281275902 40.0 38.0 41.0 33.0 41.0 20-21 38.099027912385736 40.0 37.0 41.0 33.0 41.0 22-23 37.92691841090007 40.0 37.0 41.0 33.0 41.0 24-25 37.85816961439821 40.0 37.0 41.0 33.0 41.0 26-27 37.74382303962862 40.0 37.0 41.0 32.5 41.0 28-29 37.620022700516245 39.0 37.0 41.0 32.0 41.0 30-31 37.689913499631025 39.5 37.0 41.0 33.0 41.0 32-33 37.600443604924905 40.0 36.5 41.0 33.0 41.0 34-35 37.445190851380985 39.5 36.0 41.0 32.0 41.0 36-37 37.25792315392856 39.0 36.0 41.0 31.5 41.0 38-39 37.09056026899354 39.0 36.0 41.0 31.0 41.0 40-41 36.88804048777007 39.0 36.0 41.0 31.0 41.0 42-43 36.66010023469958 39.0 35.0 40.0 30.5 41.0 44-45 36.34101445872186 39.0 35.0 40.0 30.0 41.0 46-47 35.97980694594335 39.0 35.0 40.0 29.5 41.0 48-49 35.62480260637329 38.0 35.0 40.0 28.0 41.0 50-51 35.344271827319794 38.0 34.5 40.0 27.0 41.0 52-53 35.08329761875811 38.0 34.0 40.0 27.0 41.0 54-55 34.838076627597914 38.0 34.0 40.0 26.0 41.0 56-57 34.49827453750788 38.0 34.0 40.0 26.0 41.0 58-59 34.19913962093248 37.5 33.0 40.0 25.0 41.0 60-61 33.789283275253126 37.0 33.0 40.0 24.0 41.0 62-63 33.36871674308589 36.0 32.0 39.0 23.0 40.5 64-65 32.90829573502191 36.0 32.0 39.0 22.0 40.0 66-67 32.420627330002276 35.0 31.0 38.5 20.5 40.0 68-69 31.908435071699586 35.0 31.0 38.0 19.5 40.0 70-71 31.45641353371768 35.0 30.5 37.5 18.0 39.5 72-73 30.971610600431447 34.0 30.0 37.0 17.0 39.0 74-75 30.424996401964187 34.0 29.0 36.0 12.5 39.0 76-77 29.28181650376169 32.5 28.5 35.0 11.5 37.0 78-79 29.869963796384262 34.0 29.0 35.0 9.5 37.0 80-81 29.65126521291402 34.0 29.0 35.0 7.0 37.0 82-83 29.24324017776091 34.0 29.0 35.0 4.5 36.5 84-85 28.76863892185704 33.5 29.0 35.0 2.0 36.0 86-87 28.255148380604815 33.0 27.5 35.0 2.0 36.0 88-89 27.70336357543895 33.0 26.5 35.0 2.0 35.0 90-91 27.044260624571177 32.0 25.5 34.0 2.0 35.0 92-93 26.44771924396995 32.0 25.0 34.0 2.0 35.0 94-95 25.795862199015833 32.0 23.5 34.0 2.0 35.0 96-97 25.02334736534077 31.0 19.5 34.0 2.0 35.0 98-99 23.961576565604055 31.0 9.0 34.0 2.0 35.0 100-101 21.455868147236806 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2440463957869774 1101 2 0.20987415440507107 1101 3 0.14911010839013272 1101 4 0.12664585174886867 1101 5 -0.014271389674782142 1101 6 0.05483443234910368 1101 7 -0.011960465835528566 1101 8 0.06602498379136534 1101 9 0.24037235631712406 1101 10-11 0.2375539392400441 1101 12-13 0.3320783550237394 1101 14-15 0.3162788222251933 1101 16-17 0.07144271948151015 1101 18-19 0.27549129224263424 1101 20-21 0.31821128089988804 1101 22-23 0.23767222458275938 1101 24-25 0.25272835418543593 1101 26-27 0.2265771803888299 1101 28-29 0.19512862573297696 1101 30-31 0.18972021552209384 1101 32-33 0.2972704462286657 1101 34-35 0.26868484658472624 1101 36-37 0.28428446493065707 1101 38-39 0.4935655591721897 1101 40-41 0.35040315404359035 1101 42-43 0.18064687777751232 1101 44-45 0.43071343028559284 1101 46-47 0.22158562155202333 1101 48-49 0.254830837220581 1101 50-51 0.4236183924248067 1101 52-53 0.4519136598973006 1101 54-55 0.5054264574299978 1101 56-57 0.44578028530237646 1101 58-59 0.38890496443092815 1101 60-61 0.532822121064136 1101 62-63 0.5834391387857139 1101 64-65 0.39425916482814927 1101 66-67 0.3295966972340736 1101 68-69 0.30162902495901633 1101 70-71 0.3135502798412304 1101 72-73 0.4585115685783627 1101 74-75 0.39167191008791136 1101 76-77 0.19633527550378105 1101 78-79 0.3747827592945008 1101 80-81 0.4772309174598064 1101 82-83 0.4119619732408175 1101 84-85 0.5594479966448915 1101 86-87 0.770586281871644 1101 88-89 0.847676250809414 1101 90-91 0.85934097260483 1101 92-93 1.1397154223191102 1101 94-95 1.1308130668988419 1101 96-97 1.2318900428858512 1101 98-99 1.7103582591354396 1101 100-101 2.2289558494787656 1102 1 0.12616729966341111 1102 2 0.14817921912363374 1102 3 0.08056687320707567 1102 4 0.05434935109769867 1102 5 -0.02831129709859681 1102 6 -0.04846288771943108 1102 7 0.009545386962180658 1102 8 0.0850348245058754 1102 9 0.099873230961677 1102 10-11 0.07228830930330332 1102 12-13 0.12632355346915602 1102 14-15 0.09787461828017285 1102 16-17 0.11409397134060839 1102 18-19 0.24378709677434784 1102 20-21 0.22948090260648968 1102 22-23 0.3098320700493744 1102 24-25 0.2747087112870119 1102 26-27 0.10442850879538668 1102 28-29 0.10968458628791211 1102 30-31 0.18512184562322176 1102 32-33 0.26410626221681355 1102 34-35 0.24557201101533366 1102 36-37 0.1952439795478753 1102 38-39 0.08940939209324483 1102 40-41 0.08878174798503835 1102 42-43 0.21294617831873808 1102 44-45 0.20926896957267616 1102 46-47 0.12352204092931629 1102 48-49 0.1432380080215765 1102 50-51 0.3109429818812899 1102 52-53 -0.05598023197557467 1102 54-55 0.07628506041329075 1102 56-57 0.3119658974636579 1102 58-59 0.22250486527885727 1102 60-61 0.1974561060782989 1102 62-63 0.18327705340704625 1102 64-65 0.3117164151306113 1102 66-67 0.30982452865500676 1102 68-69 0.21499702298624968 1102 70-71 0.30615837743152596 1102 72-73 0.2723672355301545 1102 74-75 0.4234340298872432 1102 76-77 0.27372892463388965 1102 78-79 0.4914119228466127 1102 80-81 0.557029325375531 1102 82-83 0.567274058234176 1102 84-85 0.6793965711412326 1102 86-87 0.7635508517032115 1102 88-89 0.61525814759365 1102 90-91 0.868456412842594 1102 92-93 1.0860479516473127 1102 94-95 1.0283286139947911 1102 96-97 1.3095961751588234 1102 98-99 1.555316915804699 1102 100-101 2.163824049149458 1103 1 0.10622194447215705 1103 2 0.16490983184308305 1103 3 0.13869383518824208 1103 4 0.07691649445312265 1103 5 -0.0991460837513145 1103 6 -0.050003406263783745 1103 7 0.026026028748020735 1103 8 0.006259100752821212 1103 9 0.07620737815386747 1103 10-11 0.037417364064381786 1103 12-13 0.08732227454810015 1103 14-15 0.16960755631063051 1103 16-17 0.1304089347169537 1103 18-19 0.14740004875667978 1103 20-21 0.09598770414119429 1103 22-23 0.2060766927676596 1103 24-25 0.21303274337630285 1103 26-27 0.1197290552434751 1103 28-29 0.02625824051416714 1103 30-31 0.04009801118536416 1103 32-33 0.09567817245980592 1103 34-35 0.17259793823310332 1103 36-37 0.08851801745044696 1103 38-39 0.12625394505219845 1103 40-41 0.16702555684447873 1103 42-43 0.1336356330333004 1103 44-45 0.033016208265848945 1103 46-47 0.15697745309069688 1103 48-49 0.09791769642990289 1103 50-51 0.07639467354505314 1103 52-53 0.06134333505151801 1103 54-55 0.049389931939856524 1103 56-57 0.24052943658701764 1103 58-59 0.10155671971438807 1103 60-61 0.0655906253202474 1103 62-63 0.18025414909359228 1103 64-65 0.26020786961264974 1103 66-67 0.2778311557913895 1103 68-69 -0.08532561391643867 1103 70-71 0.005748541365953486 1103 72-73 0.28000297556038234 1103 74-75 0.2725184386769186 1103 76-77 0.3045480154372804 1103 78-79 0.19907963590323519 1103 80-81 0.35116954162945646 1103 82-83 0.23630672885387227 1103 84-85 0.4136600288787058 1103 86-87 0.6254157511335094 1103 88-89 0.6545675302235772 1103 90-91 0.7579285672432441 1103 92-93 0.9248225127448642 1103 94-95 0.8422804800568748 1103 96-97 1.1419325313964706 1103 98-99 1.494713497597516 1103 100-101 2.050973799178525 1104 1 0.13463601606424547 1104 2 0.17607799500424193 1104 3 0.10611506366071666 1104 4 0.04051660467092688 1104 5 -0.08875774064213005 1104 6 -0.05472404634202377 1104 7 0.024703851732994053 1104 8 0.05648897181448831 1104 9 0.059313055777465706 1104 10-11 0.05965181653853335 1104 12-13 0.06990111884265104 1104 14-15 0.019129513278151933 1104 16-17 0.04081992050167571 1104 18-19 0.022564259697638533 1104 20-21 0.10881168117009565 1104 22-23 0.06728455034608061 1104 24-25 0.08453471075435459 1104 26-27 0.085053440980559 1104 28-29 -0.027912450573765568 1104 30-31 -0.006322141790342073 1104 32-33 -0.06587307275394494 1104 34-35 -0.034953048415118815 1104 36-37 -0.008685581801117337 1104 38-39 -0.09396093114924042 1104 40-41 -0.03792940521236687 1104 42-43 0.08404768849342048 1104 44-45 -0.04760178275275706 1104 46-47 0.020093322950252457 1104 48-49 0.0332539383424546 1104 50-51 0.07565608814590519 1104 52-53 0.04198409002405867 1104 54-55 0.08543956972980737 1104 56-57 0.2013788922653461 1104 58-59 0.14109402082389266 1104 60-61 0.14513560577744755 1104 62-63 0.25014864417041593 1104 64-65 0.1092758209247151 1104 66-67 0.13375552028049498 1104 68-69 -0.011802166194943453 1104 70-71 0.10166410346946009 1104 72-73 0.1893576872016034 1104 74-75 0.2456620060953334 1104 76-77 0.021417548510626716 1104 78-79 0.18094797074607882 1104 80-81 0.16879655527521464 1104 82-83 0.3862123985216108 1104 84-85 0.45114269213275904 1104 86-87 0.44302687731009627 1104 88-89 0.6219584997375875 1104 90-91 0.7218656494695068 1104 92-93 1.109843067006512 1104 94-95 1.269655220096645 1104 96-97 1.689850715053673 1104 98-99 1.8785125480362268 1104 100-101 2.37766024796775 1105 1 0.08413421930338671 1105 2 0.07300936916811196 1105 3 0.02478178620712157 1105 4 0.04417177278283191 1105 5 -0.18344879231496947 1105 6 -0.002175979212275081 1105 7 -3.138624675145252E-4 1105 8 -0.001293434451319797 1105 9 -0.007120313000513079 1105 10-11 0.022416908208910513 1105 12-13 -0.05842474057627811 1105 14-15 0.04572968793480214 1105 16-17 0.11080892161569977 1105 18-19 0.1911538633381369 1105 20-21 0.061242595788137066 1105 22-23 0.12572788878330954 1105 24-25 0.02060435607074851 1105 26-27 0.01922496219909675 1105 28-29 -0.04701771532187138 1105 30-31 -0.02546078818176767 1105 32-33 0.009077610132138147 1105 34-35 -0.018774159843530924 1105 36-37 0.20621824982674752 1105 38-39 0.06393960227480022 1105 40-41 0.12310498226615607 1105 42-43 0.17157435725883374 1105 44-45 0.2712255615401489 1105 46-47 0.30298398807828164 1105 48-49 0.16986928824542957 1105 50-51 0.27549590499032917 1105 52-53 0.3156181716584996 1105 54-55 0.3930470269452684 1105 56-57 0.42466712058213574 1105 58-59 0.2406706100094098 1105 60-61 0.29637674502227185 1105 62-63 0.19661654954783359 1105 64-65 0.25662799872363706 1105 66-67 0.2633563391785003 1105 68-69 0.1429156590177989 1105 70-71 0.2501388303978729 1105 72-73 0.315977671548783 1105 74-75 0.4557742317688884 1105 76-77 0.47383018850641534 1105 78-79 0.513877841030375 1105 80-81 0.6936736767151963 1105 82-83 0.677727223382508 1105 84-85 0.6834411620351872 1105 86-87 0.8208005994754046 1105 88-89 0.9908474660567279 1105 90-91 0.8293807345610205 1105 92-93 1.2010606003126192 1105 94-95 1.3254547080173644 1105 96-97 1.4509504769475292 1105 98-99 1.6425964339129138 1105 100-101 2.1853211889109936 1106 1 -0.21274963746845543 1106 2 -0.06493533791447348 1106 3 -0.16777427356537444 1106 4 -0.04723737807648121 1106 5 -0.20750951803861284 1106 6 -0.2394413707101748 1106 7 -0.267861753319103 1106 8 -0.1649291134674371 1106 9 -0.19156203770042168 1106 10-11 -0.18051551327152282 1106 12-13 -0.3557115469034855 1106 14-15 -0.28948864653531103 1106 16-17 -0.2078587874044331 1106 18-19 -0.053060481059532094 1106 20-21 -0.1887574673899124 1106 22-23 -0.15538009487429605 1106 24-25 -0.1328552988103553 1106 26-27 -0.34179098874037095 1106 28-29 -0.1825320262857133 1106 30-31 -0.268616584476554 1106 32-33 -0.2867832677747728 1106 34-35 -0.7864450583439648 1106 36-37 -0.4331428457916999 1106 38-39 -0.49015545945141525 1106 40-41 -0.5123091283222152 1106 42-43 -0.5142866621669029 1106 44-45 -0.43630742182052273 1106 46-47 -0.519974773591656 1106 48-49 -0.4381059035062478 1106 50-51 -0.5496953159910447 1106 52-53 -0.3707536422412261 1106 54-55 -0.500127368965245 1106 56-57 -0.6516805744680667 1106 58-59 -0.557603921834982 1106 60-61 -0.6050832616341637 1106 62-63 -0.4823678558368485 1106 64-65 -0.7336586299469161 1106 66-67 -0.4861635708761831 1106 68-69 -0.8329564569993302 1106 70-71 -0.6136847527523912 1106 72-73 -0.16502608097423277 1106 74-75 -0.386095360664509 1106 76-77 -0.43015074840373657 1106 78-79 -0.2097721136962427 1106 80-81 -0.31848455850474267 1106 82-83 -0.35126783382015603 1106 84-85 -0.25123211019066005 1106 86-87 -0.03593225924762322 1106 88-89 0.030620570961843896 1106 90-91 0.06477207578284805 1106 92-93 0.43160631202716715 1106 94-95 0.13197087869006552 1106 96-97 0.48546767413706604 1106 98-99 0.5790276983506786 1106 100-101 1.3998650396531254 1107 1 0.003529459352272113 1107 2 0.01603554490720427 1107 3 7.110049847014466E-4 1107 4 -0.019295123850824325 1107 5 -0.24902738037473426 1107 6 -0.06522106662585969 1107 7 -0.09941137350453033 1107 8 -0.022961057443339428 1107 9 0.05055957275989442 1107 10-11 0.0512004575531364 1107 12-13 -0.0013817771188087136 1107 14-15 -0.0029089552727938894 1107 16-17 -0.0015374631137490269 1107 18-19 -0.09155378631876943 1107 20-21 -0.003171808443617863 1107 22-23 -0.049788154155613995 1107 24-25 -0.07040110340069816 1107 26-27 -0.14308922170106086 1107 28-29 -0.14936368641855324 1107 30-31 -0.04585504753624292 1107 32-33 -0.05856589518424471 1107 34-35 -0.010582187108660435 1107 36-37 0.03550936504318969 1107 38-39 -0.055660385989568795 1107 40-41 -0.083781738631302 1107 42-43 0.0019761485772562537 1107 44-45 -0.008175551131969883 1107 46-47 -0.01191607391854177 1107 48-49 0.013736749728288089 1107 50-51 0.015163983940318815 1107 52-53 0.07656380828981924 1107 54-55 0.015641043025958368 1107 56-57 0.1408551676535481 1107 58-59 0.1385938609320263 1107 60-61 0.3333117455200707 1107 62-63 0.15622374752738466 1107 64-65 0.06588594155477523 1107 66-67 0.22267627882659013 1107 68-69 0.22441706416675444 1107 70-71 0.3394287961000231 1107 72-73 0.34384401429931444 1107 74-75 0.40203732432787476 1107 76-77 0.3637140538949275 1107 78-79 0.5465823643931706 1107 80-81 0.32422218742082975 1107 82-83 0.3086032735167201 1107 84-85 0.5238600806356253 1107 86-87 0.6504531623872651 1107 88-89 0.6145374887055226 1107 90-91 0.8719681950434612 1107 92-93 1.2341376336422805 1107 94-95 1.2658715103571794 1107 96-97 1.4433012769661886 1107 98-99 1.5872318094217235 1107 100-101 2.1751200912450877 1108 1 0.04583574667622514 1108 2 0.07154991054002835 1108 3 -0.035751020730991456 1108 4 -0.020919889774376088 1108 5 -0.1711257033093787 1108 6 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27.0 5 70.0 6 131.0 7 330.0 8 794.0 9 1615.0 10 2773.0 11 4225.0 12 6449.0 13 8859.0 14 11485.0 15 15738.0 16 20057.0 17 26404.0 18 24369.0 19 17307.0 20 16469.0 21 17209.0 22 18826.0 23 20706.0 24 24343.0 25 28949.0 26 35288.0 27 43586.0 28 53397.0 29 65558.0 30 79763.0 31 98274.0 32 121858.0 33 155330.0 34 204372.0 35 277894.0 36 386970.0 37 464899.0 38 242402.0 39 11577.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.7557883032264 20.15162676317262 15.697545726728034 22.395039206872937 2 15.980109332491333 21.048577688899943 39.24444008560339 23.726872893005336 3 19.800479445747 24.1873721501264 30.245188698539614 25.766959705586984 4 13.507843718068676 17.350147290230524 36.355470788533964 32.786538203166835 5 13.632180534832367 37.53567688998798 34.20049076408155 14.631651811098095 6 32.21727113003839 36.63211090481929 16.667377635885156 14.483240329257155 7 28.42755270175612 33.081636940844305 20.201864759302367 18.288945598097207 8 26.428599620541775 35.60648132828718 19.113284506359456 18.851634544811592 9 26.0091942273552 17.006250814790658 20.247712307036643 36.7368426508175 10-11 25.073824518710385 27.112963972199644 26.5725808541438 21.240630654946173 12-13 25.849837434191247 24.16862190840897 27.684329293940113 22.29721136345967 14-15 22.661645496395387 25.627224353579475 26.092018819022982 25.619111331002152 16-17 21.768874139710633 28.9982550024658 26.92341426289303 22.309456594930538 18-19 21.784043697895758 27.26916457395867 29.56082434688175 21.38596738126382 20-21 22.402870455734682 26.19659631724516 30.038581715794983 21.36195151122518 22-23 22.216964141372564 26.075321277267133 29.921857698126047 21.78585688323426 24-25 22.192102220627515 26.270023521907266 29.94424510624726 21.593629151217954 26-27 21.753272317973046 26.517083151352395 29.93269678709536 21.796947743579203 28-29 21.735843038756908 26.83951540159772 29.859334898161578 21.565306661483795 30-31 21.89693400584578 26.390525396527654 29.842297917857298 21.87024267976927 32-33 21.771485456559848 26.42232049987382 29.773895841547688 22.032298202018648 34-35 22.259801707362676 26.547125498293077 29.655668969542475 21.537403824801768 36-37 22.32787979966611 26.30820521765131 29.649056885854534 21.714858096828046 38-39 21.89778548370957 26.739346325598124 29.57978131118405 21.78308687950826 40-41 22.172033554026154 26.56634160090866 29.5195615539209 21.742063291144287 42-43 22.053118995624917 26.54877242115143 29.303044934605015 22.095063648618638 44-45 22.091541207177894 26.716203280850515 29.302264111376854 21.88999140059474 46-47 22.42449407231075 27.0990103707153 28.695900392016465 21.780595164957482 48-49 22.385543590453025 27.16746275285421 28.471327142984148 21.975666513708617 50-51 22.40595571605298 27.305723009847654 28.414755255823337 21.873566018276026 52-53 22.61014128024319 27.329509381307155 28.127516009269183 21.932833329180475 54-55 22.511524678140248 27.309610084546865 28.152467415350475 22.02639782196241 56-57 22.41925150489592 27.506555202640183 28.126772854902875 21.94742043756102 58-59 22.59353198743538 27.192479566944 28.128168215051314 22.08582023056931 60-61 22.30750309072273 27.31178285963514 28.463991535346665 21.916722514295465 62-63 22.185010403406594 27.305603407549217 28.587779914229206 21.921606274814987 64-65 22.21381462361356 27.365564026499083 28.372734981639052 22.0478863682483 66-67 22.19557527310188 27.445577964153596 28.151012254052826 22.207834508691697 68-69 22.321955035117227 27.246260733807944 28.33292734997586 22.098856881098968 70-71 22.44600255231305 27.29575615164455 28.061908541717084 22.19633275432532 72-73 22.37266431475204 27.356077456104927 27.98287373455684 22.288384494586197 74-75 22.217909893187134 27.7886881695738 27.749199461629974 22.244202475609097 76-77 22.376413948338982 27.476016749105874 27.936744736402513 22.210824566152628 78-79 22.39535097892488 27.515605109888597 27.904870723868992 22.184173187317533 80-81 22.45014876532554 27.50336580801519 27.827747175093382 22.21873825156589 82-83 22.469316485864287 27.538467641253998 27.823679653567474 22.168536219314245 84-85 22.30270595029194 27.636107324247384 27.783298326300365 22.277888399160307 86-87 22.447118840431795 27.678443639210897 27.671566507050755 22.202871013306552 88-89 22.4034440677858 27.60502776168684 27.614775349009474 22.37675282151789 90-91 22.365333742666394 27.571771169529676 27.650335207892685 22.41255987991125 92-93 22.42201497625026 27.440637610944417 27.745512985609817 22.391834427195505 94-95 22.348785817399982 27.342999059527262 27.813753706176225 22.494461416896534 96-97 22.35478586603825 27.311962263082794 27.77687633082474 22.556375540054212 98-99 22.147415280058254 27.378071716457526 27.591303063399096 22.883209940085123 100-101 22.25699735807319 27.130566846011472 28.068139861835125 22.544295934080218 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 726.0 1 512.5 2 406.5 3 530.0 4 454.0 5 451.0 6 874.5 7 1925.0 8 2997.0 9 3546.5 10 3253.0 11 2750.0 12 2850.0 13 3079.0 14 3322.5 15 3546.0 16 3738.0 17 3977.0 18 4243.5 19 4622.5 20 5277.0 21 6230.5 22 7298.0 23 8879.5 24 10609.0 25 12768.5 26 16390.5 27 20295.5 28 22860.0 29 25681.5 30 29884.0 31 34754.0 32 40622.0 33 44946.5 34 48462.0 35 53406.5 36 59524.5 37 64706.0 38 68072.5 39 71268.5 40 74143.5 41 77889.5 42 80897.5 43 83520.5 44 86767.5 45 89541.0 46 92005.5 47 96171.0 48 99296.0 49 97431.0 50 92808.5 51 87896.0 52 85348.0 53 84760.0 54 84113.0 55 77671.5 56 68779.5 57 61960.0 58 56103.0 59 49970.0 60 41298.5 61 34134.5 62 27839.0 63 20576.5 64 14940.0 65 11100.0 66 8138.0 67 6398.0 68 5013.0 69 3795.5 70 2861.5 71 1883.5 72 1252.0 73 893.5 74 596.0 75 428.5 76 302.5 77 160.5 78 93.0 79 65.0 80 37.0 81 16.0 82 10.5 83 9.5 84 5.0 85 3.0 86 3.5 87 2.5 88 1.0 89 2.0 90 2.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03875114469366463 2 3.986743281241217E-5 3 0.0 4 0.0 5 0.01542869649840351 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003907008415616392 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.9933716406206084E-5 22-23 1.7940344765585476E-4 24-25 5.182766265613582E-4 26-27 0.0014950287304654562 28-29 0.0020133053570268143 30-31 2.9900574609309125E-4 32-33 0.0 34-35 0.0 36-37 1.9933716406206084E-5 38-39 0.0 40-41 0.0 42-43 0.009807388471853393 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.9933716406206084E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 7.973486562482434E-5 74-75 3.986743281241217E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3953601484344258E-4 84-85 2.9900574609309125E-4 86-87 0.0 88-89 0.0 90-91 0.0066977287124852436 92-93 0.0029900574609309123 94-95 0.0 96-97 0.0 98-99 1.7940344765585476E-4 100-101 0.01176089267966159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2508313.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.022978209647405 #Duplication Level Percentage of deduplicated Percentage of total 1 74.09024054177316 38.54394969252199 2 14.168608722070806 14.741864456186192 3 4.906759828361791 7.657927788925162 4 2.1799571197529897 4.536314469555021 5 1.2300687546557223 3.1995920009911383 6 0.7229427110683951 2.2565779742840726 7 0.485490926084133 1.7679678708059476 8 0.3479724584840833 1.4482050900219925 9 0.260854785765906 1.2213398552203774 >10 1.455398989830578 13.93000787424939 >50 0.10193459264284407 3.6391244215009597 >100 0.045552925231296594 4.211403312320545 >500 0.003220746539741621 1.1248379789672585 >1k 7.66844414222617E-4 0.6084960169229726 >5k 1.5336888284452337E-4 0.6659427004848345 >10k+ 7.668444142226169E-5 0.4464484970422203 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11191 0.4461564406037045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9861 0.39313275496319644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6832 0.2723743009743999 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2549 0.10162208623883862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.9933716406206084E-5 14-15 0.0 0.0 0.0 0.0 5.980114921861825E-5 16-17 0.0 0.0 0.0 1.196022984372365E-4 1.196022984372365E-4 18-19 0.0 0.0 0.0 1.196022984372365E-4 1.5946973124964867E-4 20-21 0.0 0.0 0.0 1.196022984372365E-4 2.591383132806791E-4 22-23 0.0 0.0 0.0 2.192708804682669E-4 3.1893946249929734E-4 24-25 0.0 0.0 0.0 3.787406117179156E-4 3.1893946249929734E-4 26-27 0.0 0.0 0.0 5.980114921861825E-4 3.3887317890550343E-4 28-29 0.0 0.0 0.0 0.0013156252828096016 3.588068953117095E-4 30-31 0.0 0.0 0.0 0.0029701237445247065 3.588068953117095E-4 32-33 0.0 0.0 0.0 0.0060797835038928555 3.588068953117095E-4 34-35 0.0 0.0 0.0 0.009747587322634775 3.588068953117095E-4 36-37 0.0 0.0 0.0 0.013973535200750464 3.787406117179156E-4 38-39 0.0 0.0 0.0 0.018956964302301985 3.986743281241217E-4 40-41 0.0 0.0 0.0 0.026491909103847886 3.986743281241217E-4 42-43 0.0 0.0 0.0 0.036538502172575746 3.986743281241217E-4 44-45 0.0 0.0 0.0 0.047880786807707014 4.385417609365338E-4 46-47 0.0 0.0 0.0 0.061196509367052676 4.983429101551521E-4 48-49 0.0 0.0 0.0 0.0760471280896762 5.182766265613582E-4 50-51 0.0 0.0 0.0 0.09586124219744505 5.980114921861825E-4 52-53 0.0 0.0 0.0 0.12175513980910675 5.980114921861825E-4 54-55 0.0 0.0 0.0 0.15614080060981225 5.980114921861825E-4 56-57 0.0 0.0 0.0 0.18976898018708194 5.980114921861825E-4 58-59 0.0 0.0 0.0 0.2319088566698016 5.980114921861825E-4 60-61 0.0 0.0 0.0 0.2822614243118782 5.980114921861825E-4 62-63 0.0 0.0 0.0 0.32798936974771487 6.378789249985947E-4 64-65 0.0 0.0 0.0 0.37385685119839507 6.578126414048008E-4 66-67 0.0 0.0 0.0 0.44314644942636744 6.777463578110069E-4 68-69 0.0 0.0 0.0 0.5239378020207207 6.777463578110069E-4 70-71 0.0 0.0 0.0 0.595340374187751 6.777463578110069E-4 72-73 0.0 3.986743281241217E-5 0.0 0.6788825796461606 7.17613790623419E-4 74-75 0.0 3.986743281241217E-5 0.0 0.7770361992303194 7.574812234358312E-4 76-77 0.0 3.986743281241217E-5 0.0 0.8858344233753921 7.574812234358312E-4 78-79 0.0 3.986743281241217E-5 0.0 1.014865369672764 7.774149398420372E-4 80-81 0.0 3.986743281241217E-5 0.0 1.1577901163052617 7.973486562482434E-4 82-83 0.0 3.986743281241217E-5 0.0 1.31395085063148 8.770835218730677E-4 84-85 0.0 3.986743281241217E-5 0.0 1.4731614435678482 8.770835218730677E-4 86-87 0.0 3.986743281241217E-5 0.0 1.6466246437346534 9.169509546854799E-4 88-89 0.0 3.986743281241217E-5 0.0 1.8469186261842123 9.56818387497892E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8635 0.0 37.85894 1 GTATCAA 12540 0.0 32.511135 1 ATCAACG 15680 0.0 25.99284 3 TCAACGC 15710 0.0 25.9432 4 TATCAAC 15830 0.0 25.74654 2 CAACGCA 15980 0.0 25.504862 5 AACGCAG 16410 0.0 24.978792 6 GAGTACT 11060 0.0 22.311779 12-13 ACGCAGA 18695 0.0 21.673418 7 CGCAGAG 18745 0.0 21.564924 8 GTACATG 9685 0.0 21.341846 1 GTACTTT 11620 0.0 21.010006 14-15 TACATGG 9580 0.0 20.875124 2 AGAGTAC 15905 0.0 20.562412 10-11 AGTACTT 11440 0.0 20.345758 12-13 ACATGGG 9530 0.0 20.037596 3 GCAGAGT 20050 0.0 19.640112 9 TAAGGTG 1105 0.0 19.34473 5 GTAAGGT 950 0.0 19.000824 4 GGACCGA 640 0.0 18.553642 6 >>END_MODULE