FastQCFastQC Report
Thu 26 May 2016
SRR937859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647485
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68400.4151782869039779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55810.338758774738465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.21499437020670903No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22480.13645040774271086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.044.525051
GTATCAA76450.037.5063061
TCAACGC94200.030.2839054
ATCAACG94150.030.2448843
TATCAAC95350.029.9141042
CAACGCA95450.029.6880665
AACGCAG97300.029.0987876
ACGCAGA109400.025.7508777
CGCAGAG110100.025.4569388
GTACATG73500.025.3608131
TACATGG74650.024.7722382
GAGTACT62250.023.81057512-13
GCAGAGT116100.023.5282869
ACATGGG75300.023.1063613
GTACTTT66250.022.30124914-15
CATGGGA41400.020.3275244
GTAAGGT6800.020.2768574
CAGAGTA113600.020.26204710-11
AGTACTT66200.020.1293412-13
AGAGTAC104150.020.07064210-11
Produced by FastQC (version 0.11.5)