FastQCFastQC Report
Thu 26 May 2016
SRR937859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647485
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82260.49930651872399445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72320.43897213024701287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47190.2864365988157707No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.11981899683456906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52750.042.528961
GTATCAA83200.035.3616561
ATCAACG104000.028.1479423
TATCAAC105700.027.8301142
TCAACGC105600.027.721464
CAACGCA106400.027.5130255
AACGCAG109900.026.7519196
GTACATG71050.025.420541
TACATGG73050.024.2654252
ACGCAGA124600.023.520277
CGCAGAG125250.023.3982078
ACATGGG72650.023.2215943
GAGTACT76300.022.72183812-13
GCAGAGT133850.021.7529059
TAAGGTG7650.021.7423215
GTACTTT79700.021.66247614-15
GGACCGA4850.021.5448236
GTAAGGT6700.021.2787344
AGTACTT79650.020.93131412-13
AGAGTAC115950.020.74836310-11
Produced by FastQC (version 0.11.5)