FastQCFastQC Report
Thu 26 May 2016
SRR937858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636031
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68390.41802386385099055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54820.33507922527140377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35490.21692742985921415No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21890.1337994206711242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46600.043.9797361
GTATCAA74400.037.389031
ATCAACG95800.029.0174163
TATCAAC96200.028.9479312
TCAACGC96450.028.8236274
CAACGCA98450.028.382895
AACGCAG101800.027.485456
GTACATG75800.025.092971
ACGCAGA114400.024.2528117
CGCAGAG114950.024.17818
TACATGG76450.023.994122
ACATGGG75800.022.9445883
GAGTACT63600.022.74462712-13
GCAGAGT120250.022.2432659
GTACTTT67000.021.198514-15
AGTACTT66100.020.84227812-13
AGAGTAC105300.020.50473410-11
CAGAGTA117550.019.43887310-11
TAACACG1250.006016067719.0088144
CATGGGG37350.018.8306874
Produced by FastQC (version 0.11.5)