FastQCFastQC Report
Thu 26 May 2016
SRR937858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636031
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82790.5060417559324977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72390.44247327831807587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46730.2856302845117238No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.11772393065901564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52500.043.6272931
GTATCAA83250.035.675191
TATCAAC104350.028.3635062
ATCAACG104850.028.2282473
TCAACGC105350.028.0942754
GTATAGG4450.027.7641181
CAACGCA107550.027.5195885
AACGCAG109150.027.1103886
GTACATG71950.024.7008171
TACATGG73050.024.3879532
ACATGGG72550.023.5083073
ACGCAGA125950.023.30717
GAGTACT76950.023.27183712-13
CGCAGAG127300.023.0599338
GTACTTT82250.021.74204814-15
GCAGAGT135050.021.3848869
AGTACTT80500.021.15393612-13
GGACCGA4500.021.1100316
TAAGGTG8050.020.6555375
ACGTAAC1656.305019E-520.1597251
Produced by FastQC (version 0.11.5)