##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937858_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1636031 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23754928849148 33.0 31.0 34.0 31.0 34.0 2 32.43660358513989 34.0 31.0 34.0 31.0 34.0 3 32.52772899780016 34.0 31.0 34.0 31.0 34.0 4 36.15092317932851 37.0 35.0 37.0 35.0 37.0 5 36.067837345380376 37.0 35.0 37.0 35.0 37.0 6 36.10918680636247 37.0 35.0 37.0 35.0 37.0 7 36.05836625345119 37.0 35.0 37.0 35.0 37.0 8 36.03537096790953 37.0 35.0 37.0 35.0 37.0 9 37.71107699059492 39.0 37.0 39.0 35.0 39.0 10-11 37.63875776192505 39.0 37.0 39.0 35.0 39.0 12-13 37.54882792563221 39.0 37.0 39.0 35.0 39.0 14-15 38.76776815353743 40.0 38.0 41.0 35.0 41.0 16-17 38.72742509157834 40.0 38.0 41.0 35.0 41.0 18-19 38.7151667663999 40.0 38.0 41.0 34.5 41.0 20-21 38.59765615688211 40.0 38.0 41.0 34.0 41.0 22-23 38.44704073455821 40.0 38.0 41.0 34.0 41.0 24-25 38.5247904837989 40.0 38.0 41.0 34.0 41.0 26-27 38.4038477877253 40.0 38.0 41.0 34.0 41.0 28-29 38.25954153680462 40.0 38.0 41.0 34.0 41.0 30-31 38.2927857112732 40.0 38.0 41.0 34.0 41.0 32-33 38.21441647499344 40.0 38.0 41.0 34.0 41.0 34-35 38.08552221809978 40.0 37.0 41.0 34.0 41.0 36-37 37.91582188846055 40.0 37.0 41.0 33.0 41.0 38-39 37.76546899172449 40.0 37.0 41.0 33.0 41.0 40-41 37.5859302177037 40.0 37.0 41.0 33.0 41.0 42-43 37.36273609729889 40.0 37.0 41.0 32.5 41.0 44-45 37.035464486919864 40.0 36.0 41.0 31.5 41.0 46-47 36.66772420571493 39.5 36.0 41.0 31.0 41.0 48-49 36.325309544868034 39.0 36.0 41.0 30.0 41.0 50-51 36.04318316706713 39.0 35.0 41.0 29.5 41.0 52-53 35.78873994441426 39.0 35.0 40.0 29.0 41.0 54-55 35.55448307519845 39.0 35.0 40.0 28.0 41.0 56-57 35.27977159357005 38.0 35.0 40.0 28.0 41.0 58-59 35.000622848833544 38.0 34.0 40.0 27.0 41.0 60-61 34.63637852827972 38.0 34.0 40.0 26.0 41.0 62-63 34.27381907800036 37.0 34.0 40.0 26.0 41.0 64-65 33.87454547010417 37.0 33.0 39.5 25.5 41.0 66-67 33.42275940981558 36.0 33.0 39.0 24.5 40.0 68-69 32.97617649054327 35.5 32.0 39.0 23.0 40.0 70-71 32.54363150820492 35.0 32.0 38.0 22.0 40.0 72-73 32.04679587367232 35.0 31.0 37.5 20.5 39.5 74-75 31.535109053557054 35.0 31.0 37.0 19.5 39.0 76-77 30.326978523023094 33.5 29.5 35.5 18.5 38.0 78-79 30.927479980513816 34.0 31.0 36.0 18.5 38.0 80-81 30.718732102264568 34.0 31.0 35.5 18.0 37.0 82-83 30.357194637509927 34.0 30.5 35.0 16.0 37.0 84-85 29.96053314393187 34.0 30.0 35.0 9.5 36.5 86-87 29.53423284766609 34.0 29.5 35.0 7.0 36.0 88-89 29.154155086303376 34.0 29.0 35.0 2.0 36.0 90-91 28.606107708228024 34.0 29.0 35.0 2.0 35.0 92-93 28.106094872285425 33.0 28.0 35.0 2.0 35.0 94-95 27.612140601247773 33.0 27.0 35.0 2.0 35.0 96-97 27.031734117507554 33.0 26.0 35.0 2.0 35.0 98-99 26.121695738039193 32.5 24.5 34.0 2.0 35.0 100-101 23.435416260449834 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24540493159349808 1101 2 0.28836731849792585 1101 3 0.1807128466827379 1101 4 0.0969833857079081 1101 5 0.13013140293590908 1101 6 0.07743661505170962 1101 7 0.120467963821838 1101 8 0.12795455817781942 1101 9 0.2480881067239764 1101 10-11 0.20571025144548116 1101 12-13 0.30825802851920514 1101 14-15 0.2653780430636914 1101 16-17 0.22807165900344017 1101 18-19 0.2594052059364955 1101 20-21 0.3251944021142492 1101 22-23 0.32405551487928363 1101 24-25 0.28488127319744194 1101 26-27 0.1486107899647635 1101 28-29 0.34224569867079424 1101 30-31 0.3729928475588409 1101 32-33 0.4397303128960175 1101 34-35 0.36114358709260586 1101 36-37 0.40222855313322015 1101 38-39 0.3645986981004299 1101 40-41 0.27453949155587054 1101 42-43 0.41582188161944345 1101 44-45 0.405961449508915 1101 46-47 0.5435373475475131 1101 48-49 0.6357903634740509 1101 50-51 0.710197498057866 1101 52-53 0.4900966268445828 1101 54-55 0.5764315603126775 1101 56-57 0.6204484811201567 1101 58-59 0.6694835395747774 1101 60-61 0.5400686341109378 1101 62-63 0.5787816638150929 1101 64-65 0.5641762223417643 1101 66-67 0.4705692566946027 1101 68-69 0.4109219848009147 1101 70-71 0.4371301110851107 1101 72-73 0.38128677565005376 1101 74-75 0.3321744457052951 1101 76-77 0.3517902554297301 1101 78-79 0.49225656428335185 1101 80-81 0.6490750535710248 1101 82-83 0.7311177839915324 1101 84-85 0.7793578876739637 1101 86-87 0.5220434080277236 1101 88-89 0.6860035907959947 1101 90-91 0.7014863343627233 1101 92-93 0.7714461902503906 1101 94-95 0.5724722969919753 1101 96-97 0.699622542382091 1101 98-99 0.8864121858855043 1101 100-101 1.3506333126137342 1102 1 0.2621446532380318 1102 2 0.29314287440897857 1102 3 0.19382946802332413 1102 4 0.08709056855865072 1102 5 0.10325954243358382 1102 6 0.17540813960778223 1102 7 0.1977413600784388 1102 8 0.2117510707358079 1102 9 0.23789709728549013 1102 10-11 0.22727896045203977 1102 12-13 0.22507901716102197 1102 14-15 0.35725582261896704 1102 16-17 0.20307533841473457 1102 18-19 0.25800095061735107 1102 20-21 0.3149125272142328 1102 22-23 0.320692532956393 1102 24-25 0.15243398476359005 1102 26-27 0.28100112751075557 1102 28-29 0.24590079385556862 1102 30-31 0.31262810591750423 1102 32-33 0.28700274930618974 1102 34-35 0.21838466851957605 1102 36-37 0.27873983132870706 1102 38-39 0.31943920361954525 1102 40-41 0.39373833974016037 1102 42-43 0.3598852314834602 1102 44-45 0.45420141111505785 1102 46-47 0.5059062485233525 1102 48-49 0.3942226143139109 1102 50-51 0.34820325713641864 1102 52-53 0.420052793857856 1102 54-55 0.4128791686953335 1102 56-57 0.38205846352297357 1102 58-59 0.2670173741612345 1102 60-61 0.35604943718244186 1102 62-63 0.2733319757651742 1102 64-65 0.4180290458900018 1102 66-67 0.3431803589182394 1102 68-69 0.43559067781003336 1102 70-71 0.2596877818577852 1102 72-73 0.353179912748125 1102 74-75 0.4247637914099833 1102 76-77 0.2787283453353453 1102 78-79 0.42356675465288873 1102 80-81 0.449778301051424 1102 82-83 0.38033966849001644 1102 84-85 0.4334420342105183 1102 86-87 0.37138527332927396 1102 88-89 0.4584432004584471 1102 90-91 0.552019369077172 1102 92-93 0.6658925188378184 1102 94-95 0.6510254884813378 1102 96-97 0.8250037613870482 1102 98-99 0.8646585464678118 1102 100-101 1.1553807130296683 1103 1 0.2679533899558635 1103 2 0.2847083997926845 1103 3 0.17880703590998337 1103 4 0.10359588142760146 1103 5 0.11644962507821788 1103 6 0.19755689993836256 1103 7 0.16174136007843742 1103 8 0.14296240485623457 1103 9 0.18983098158300749 1103 10-11 0.19968509976726523 1103 12-13 0.1655678011043591 1103 14-15 0.2196336266331329 1103 16-17 0.30188761704519607 1103 18-19 0.3503456967802805 1103 20-21 0.26105656499463237 1103 22-23 0.39965829446760637 1103 24-25 0.3918271370658388 1103 26-27 0.25475083235136964 1103 28-29 0.1655513251424594 1103 30-31 0.2768902074523325 1103 32-33 0.32436992758245964 1103 34-35 0.24635633321851458 1103 36-37 0.24599602967581546 1103 38-39 0.09904250940466852 1103 40-41 0.14773597846507158 1103 42-43 0.20790176040907937 1103 44-45 0.20578818797455511 1103 46-47 0.2432191174725844 1103 48-49 0.18304197676964407 1103 50-51 0.22998838110336095 1103 52-53 0.06422988948950348 1103 54-55 0.0247469372903808 1103 56-57 0.10764524038248169 1103 58-59 0.14411536707741135 1103 60-61 0.03195026362872966 1103 62-63 0.13962713515124392 1103 64-65 0.28125454766095714 1103 66-67 0.1594448217281581 1103 68-69 0.08312550661758422 1103 70-71 0.20818128835129102 1103 72-73 0.20030151841518062 1103 74-75 0.2234391160853093 1103 76-77 0.28536352833416245 1103 78-79 0.3410519966835892 1103 80-81 0.2307558689380862 1103 82-83 0.2935722540862358 1103 84-85 0.36442196337226207 1103 86-87 0.3901078235063693 1103 88-89 0.4219142748386133 1103 90-91 0.4399721435753996 1103 92-93 0.41341554126993074 1103 94-95 0.6356866455061301 1103 96-97 0.6416790860623749 1103 98-99 0.7646042371407766 1103 100-101 0.9580111734783081 1104 1 0.2730231864695085 1104 2 0.3226363809024875 1104 3 0.18607316550231445 1104 4 0.0956352591621652 1104 5 0.11354143700960151 1104 6 0.2231315926253572 1104 7 0.1546748971296239 1104 8 0.14831867959753708 1104 9 0.23821107742299574 1104 10-11 0.2639603966628883 1104 12-13 0.2298387574207652 1104 14-15 0.25925009305441193 1104 16-17 0.2902071183994579 1104 18-19 0.31441004152644325 1104 20-21 0.17939724837542315 1104 22-23 0.19267343440787954 1104 24-25 0.23899968377046577 1104 26-27 0.29537431314864193 1104 28-29 0.30037405588764443 1104 30-31 0.4673721853674664 1104 32-33 0.2382441549593608 1104 34-35 0.19620094048291037 1104 36-37 0.28241782980082775 1104 38-39 0.42241131973872115 1104 40-41 0.43996981414810676 1104 42-43 0.33587071658659085 1104 44-45 0.436520356875171 1104 46-47 0.4931106029924095 1104 48-49 0.3844231490732781 1104 50-51 0.5424710187865287 1104 52-53 0.5368278129564104 1104 54-55 0.43204952774804184 1104 56-57 0.550801015089057 1104 58-59 0.609850834818225 1104 60-61 0.6016078787408858 1104 62-63 0.7421299131219428 1104 64-65 0.7675947448968756 1104 66-67 0.7729336056714828 1104 68-69 0.6423049635243174 1104 70-71 0.5375835037829191 1104 72-73 0.6836814406320002 1104 74-75 0.5169838066888239 1104 76-77 0.5182356027837791 1104 78-79 0.6805721022464724 1104 80-81 0.7788451459712107 1104 82-83 0.8422865745251542 1104 84-85 0.8342766407804199 1104 86-87 0.7041400479664013 1104 88-89 0.89948216149741 1104 90-91 0.9686156257616609 1104 92-93 1.0107790734596662 1104 94-95 1.0435014243102145 1104 96-97 1.2934648003480866 1104 98-99 1.5110970491416076 1104 100-101 1.7113230354131623 1105 1 0.24751428717297586 1105 2 0.2570906668021635 1105 3 0.274838543173729 1105 4 0.15473408849339165 1105 5 0.17480354161784106 1105 6 0.16164858010946404 1105 7 0.15944708048223077 1105 8 0.1967328184670194 1105 9 0.3796966282327716 1105 10-11 0.18212427502326278 1105 12-13 0.3303878781786693 1105 14-15 0.287438753178769 1105 16-17 0.28649677208456836 1105 18-19 0.28814227824047123 1105 20-21 0.248455302782709 1105 22-23 0.2693265698688023 1105 24-25 0.29020761584343546 1105 26-27 0.18929581497889103 1105 28-29 0.1640321286164479 1105 30-31 0.33790392930895763 1105 32-33 0.36945915799386597 1105 34-35 0.31803308291745935 1105 36-37 0.34834786640569604 1105 38-39 0.40918571121616054 1105 40-41 0.3714191799563338 1105 42-43 0.5246267426489553 1105 44-45 0.41660214528452144 1105 46-47 0.36610549396028347 1105 48-49 0.6160570183110607 1105 50-51 0.6405028390676932 1105 52-53 0.5320501426903306 1105 54-55 0.4064156222489217 1105 56-57 0.32620876432852697 1105 58-59 0.5958143559089706 1105 60-61 0.6544921821605243 1105 62-63 0.5523277950779146 1105 64-65 0.48234657418611704 1105 66-67 0.5536989680749684 1105 68-69 0.5283684895771401 1105 70-71 0.5980118361047531 1105 72-73 0.7189820948629304 1105 74-75 0.4833972165145006 1105 76-77 0.523473935220121 1105 78-79 0.7148801022617413 1105 80-81 0.5471401854197353 1105 82-83 0.661816980614006 1105 84-85 0.6390849423373659 1105 86-87 0.661310632766412 1105 88-89 0.7963253254711944 1105 90-91 0.873588248449046 1105 92-93 1.0549780699747586 1105 94-95 0.9032124977604212 1105 96-97 1.0918839490081353 1105 98-99 1.1924829576027136 1105 100-101 1.3113272818070705 1106 1 0.11232114341452615 1106 2 0.12479255805865819 1106 3 0.1029283691222247 1106 4 0.1144032558713377 1106 5 0.11511257828662025 1106 6 0.09251547671512128 1106 7 0.013500711837785673 1106 8 0.05354172452646111 1106 9 0.1366467920351795 1106 10-11 -0.09519678602370618 1106 12-13 -0.09975525967325183 1106 14-15 0.1833239766871202 1106 16-17 0.15373778707718344 1106 18-19 0.17333583795223717 1106 20-21 0.1049222952240001 1106 22-23 0.14439083465469338 1106 24-25 0.23164388597349017 1106 26-27 0.2063648748745095 1106 28-29 0.17369721965199147 1106 30-31 0.21628666957607123 1106 32-33 0.15497366727711182 1106 34-35 0.1791154932504 1106 36-37 0.090272964861839 1106 38-39 0.029354621534956493 1106 40-41 0.024828582830402013 1106 42-43 -0.07618536458713976 1106 44-45 0.12759113250477583 1106 46-47 0.18615244676954745 1106 48-49 0.3586592887505944 1106 50-51 0.3425904255235892 1106 52-53 0.44473322453828956 1106 54-55 0.6987093385255037 1106 56-57 0.4646796201441319 1106 58-59 0.2077814989253639 1106 60-61 0.3766661537991638 1106 62-63 0.4150840015172008 1106 64-65 0.4940190558800097 1106 66-67 0.576255283993163 1106 68-69 0.37006331628267475 1106 70-71 0.47227830244830216 1106 72-73 0.5329461465143552 1106 74-75 0.5478924405386358 1106 76-77 0.5313246379316361 1106 78-79 0.776954478040615 1106 80-81 0.6803517276248527 1106 82-83 0.4606677848181313 1106 84-85 0.6576489384174202 1106 86-87 0.6409630289070307 1106 88-89 0.27652178853703546 1106 90-91 0.4240209230787251 1106 92-93 0.37436116130646013 1106 94-95 0.8016999251557735 1106 96-97 0.8239135182968091 1106 98-99 0.9635971634064298 1106 100-101 1.0256455592945137 1107 1 0.24823481415192816 1107 2 0.3045503182119518 1107 3 0.25194616130424663 1107 4 0.07922175637341411 1107 5 0.14362509949045688 1107 6 0.16653699355693163 1107 7 0.2380833328090617 1107 8 0.2575081030414239 1107 9 0.32741484694768985 1107 10-11 0.2800100745657872 1107 12-13 0.2972730965124555 1107 14-15 0.36220017834921947 1107 16-17 0.3650679683127791 1107 18-19 0.3522090331624952 1107 20-21 0.5166815973530419 1107 22-23 0.3901682730374674 1107 24-25 0.3907459857462712 1107 26-27 0.42516007604287864 1107 28-29 0.5092937431246938 1107 30-31 0.48888900752664455 1107 32-33 0.4094631350081883 1107 34-35 0.4111958710745469 1107 36-37 0.1335608606862806 1107 38-39 0.3634966904147703 1107 40-41 0.4099781137237031 1107 42-43 0.36136662197602476 1107 44-45 0.3710091742891066 1107 46-47 0.09870344788460983 1107 48-49 0.056105675362928764 1107 50-51 0.03429415506050759 1107 52-53 -0.01112838781515535 1107 54-55 -0.10832756266444932 1107 56-57 -0.12923425235959485 1107 58-59 -0.10921945178152015 1107 60-61 0.10004452378114337 1107 62-63 0.1540414709131923 1107 64-65 0.10925936157602933 1107 66-67 0.17132530425665493 1107 68-69 -0.12413957645848228 1107 70-71 0.06346766147945004 1107 72-73 0.2176174511340676 1107 74-75 0.16150792603718145 1107 76-77 0.15311232764709715 1107 78-79 0.3003423350484198 1107 80-81 0.27914373527326575 1107 82-83 0.276657565554256 1107 84-85 0.2855019777063994 1107 86-87 0.3364355128575909 1107 88-89 0.31668297444198856 1107 90-91 0.43794714207803054 1107 92-93 0.4895389996141368 1107 94-95 0.8177340009490912 1107 96-97 0.9366143743338604 1107 98-99 1.1540532154273997 1107 100-101 1.229558823826867 1108 1 0.22316589032750755 1108 2 0.19975956235141723 1108 3 0.1918491964670963 1108 4 0.1383589914436456 1108 5 0.13623993003947987 1108 6 0.14765602170132297 1108 7 0.08555475221027109 1108 8 0.16370604346413842 1108 9 0.19018536466392533 1108 10-11 0.3074818152739027 1108 12-13 0.24995820945532898 1108 14-15 0.29593796257486815 1108 16-17 0.20623583510731436 1108 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2316 84-85 -0.18071683018090567 2316 86-87 -0.1362094110561074 2316 88-89 0.05434474077859264 2316 90-91 -0.10214412634213943 2316 92-93 0.16544733611356222 2316 94-95 0.0782037830549811 2316 96-97 0.009770297176839193 2316 98-99 0.2955662279336302 2316 100-101 1.090787136063998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 11.0 5 35.0 6 56.0 7 149.0 8 394.0 9 752.0 10 1304.0 11 2132.0 12 3342.0 13 4770.0 14 6485.0 15 9075.0 16 12529.0 17 17655.0 18 16003.0 19 10015.0 20 8197.0 21 8253.0 22 8694.0 23 9190.0 24 10670.0 25 12933.0 26 15484.0 27 19274.0 28 24033.0 29 30466.0 30 38943.0 31 48222.0 32 62374.0 33 82111.0 34 114113.0 35 164552.0 36 250596.0 37 363433.0 38 260208.0 39 19572.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98934577416612 19.53818849426755 15.325223804769733 22.1472419267966 2 15.571037468116437 20.30884500354822 40.471665879191775 23.648451649143567 3 19.621571962878452 24.57881299315233 29.89894445765392 25.900670586315293 4 13.137244594835785 17.11920578376409 36.45664988618777 33.28689973521236 5 13.57179887852844 37.7045291702431 33.90813030198544 14.81554164924302 6 32.01925880377573 36.45401584688799 16.923579076435594 14.603146272900696 7 28.71241437356627 33.18910216248959 20.021197642342965 18.07728582160118 8 26.671866242143334 35.504583959594896 18.986926286849087 18.836623511412682 9 26.523397172791956 17.089223859450094 20.095401615250566 36.29197735250738 10-11 25.155788612807463 27.10624065191919 26.510133365443565 21.22783736982979 12-13 26.03610264096415 24.41077168378847 27.56607758861767 21.987048086629713 14-15 22.718395922815642 25.77448104589705 26.161393029838674 25.34573000144863 16-17 21.83898104620267 29.129490822606662 26.89832894364471 22.13319918754596 18-19 21.808602648727316 27.127206024824712 29.83999080702016 21.22420051942781 20-21 22.40969150340061 26.094615566575452 30.374668939647233 21.12102399037671 22-23 22.295754330631652 25.934982457427168 30.204153983447636 21.56510922849354 24-25 22.043622645292174 26.291952903092913 30.293130142399505 21.371294309215415 26-27 21.675075274048883 26.608352454918087 30.243725328876998 21.472846942156036 28-29 21.518930473046364 26.839823399936307 30.23343946533432 21.407806661683008 30-31 21.61905856307124 26.541061874744425 30.207801685909374 21.63207787627496 32-33 21.517043381207323 26.598212381061238 30.137051192795244 21.747693044936188 34-35 22.18979958203726 26.53537738588083 29.89582715730937 21.378995874772542 36-37 22.106335393400247 26.283120552116678 29.867343589455214 21.74320046502786 38-39 21.757567181745305 26.548590076517613 29.968334903701248 21.725507838035828 40-41 22.048810453401465 26.64180963281373 29.768630932643042 21.540748981141764 42-43 21.85180690815425 26.673112068377907 29.596712050404957 21.878368973062887 44-45 21.98916829484536 26.850966409244815 29.488081059613496 21.67178423629633 46-47 22.248294806149758 27.159509813689347 28.870449276327893 21.721746103832995 48-49 22.323201699723295 27.24401310244121 28.602300323160136 21.830484874675356 50-51 22.425186319819122 27.39052621863522 28.464008322580685 21.72027913896497 52-53 22.57714633070716 27.539187075057587 28.173649574381404 21.710017019853847 54-55 22.229010330488848 27.660417192583758 28.293504218440845 21.817068258486543 56-57 22.32460754105515 27.794766725080393 28.233236411779483 21.647389322084972 58-59 22.43533282682296 27.27964812402699 28.24127415678554 22.043744892364508 60-61 22.138944799945723 27.46622771817893 28.709358196757883 21.68546928511746 62-63 22.153064336800465 27.545474382820373 28.740897941420425 21.560563338958737 64-65 22.077851825546094 27.419468213010635 28.58680550674162 21.915874454701655 66-67 22.029992096726776 27.667904825764307 28.339224623494296 21.962878454014625 68-69 22.05428510130322 27.572646939632488 28.44698963160188 21.926078327462413 70-71 22.116923283802866 27.62082602397267 28.259353434839213 22.002897257385257 72-73 22.259663783876956 27.576708509802078 28.13708908938767 22.026538616933298 74-75 22.1989986742305 28.12755381774551 27.80344626721621 21.870001240807785 76-77 22.25058693875605 27.571788065140574 28.131251791683653 22.04637320441972 78-79 22.36993064312351 27.668088196372807 27.990148108440486 21.971833052063193 80-81 22.244029754955594 28.068586761856444 27.82390909702145 21.863474386166512 82-83 22.27720729743499 28.141726204684048 27.721188178847246 21.859878319033715 84-85 22.16985615444258 28.16340576598592 27.67478257521047 21.991955504361027 86-87 22.081580361252325 28.128501232556108 27.720532190404708 22.069386215786864 88-89 22.16097983473418 28.110347542314294 27.544404720937436 22.18426790201408 90-91 22.23070572596768 28.07236218135537 27.60758979481366 22.089342297863286 92-93 22.33461410569245 27.681675705015675 27.922783240993844 22.060926948298036 94-95 22.129378966535477 27.732359594653154 27.89354235952742 22.24471907928395 96-97 22.204805410166433 27.72691959993423 27.765702483632644 22.302572506266692 98-99 21.9917004839245 27.853562257678643 27.597602443522273 22.557134814874583 100-101 21.881616943777814 27.925241278205164 27.934472712400172 22.25866906561685 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 627.0 1 408.0 2 259.5 3 336.5 4 299.0 5 286.5 6 517.0 7 1131.5 8 1820.0 9 2229.5 10 2258.5 11 2128.0 12 2256.5 13 2509.5 14 2709.0 15 2879.0 16 2944.0 17 3015.0 18 3170.5 19 3364.0 20 3575.5 21 3929.0 22 4510.5 23 5326.5 24 6465.5 25 7931.5 26 9952.5 27 12533.0 28 14774.5 29 17522.0 30 20665.5 31 23156.0 32 26067.0 33 29079.0 34 32363.5 35 35627.5 36 39859.5 37 43690.0 38 45992.5 39 47522.0 40 48515.0 41 51616.0 42 54543.0 43 56682.0 44 59229.0 45 61639.5 46 62418.0 47 63722.5 48 65937.0 49 64096.5 50 60929.0 51 57383.0 52 54107.0 53 53045.5 54 52233.0 55 48917.5 56 43146.0 57 38381.0 58 34842.5 59 30796.0 60 25307.0 61 20287.0 62 16439.5 63 12338.0 64 8827.5 65 6565.5 66 4905.5 67 3832.5 68 2843.0 69 1884.5 70 1357.5 71 966.0 72 754.5 73 615.0 74 494.5 75 384.0 76 249.0 77 149.0 78 94.0 79 69.0 80 43.0 81 23.5 82 16.5 83 10.0 84 7.0 85 6.5 86 2.0 87 0.0 88 0.5 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020904249369357916 2 0.0 3 0.0 4 1.8337060850313961E-4 5 0.019742902182171364 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003453479793475796 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.723588978448452E-4 24-25 0.0 26-27 5.501118255094188E-4 28-29 0.0011002236510188376 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.52808840419283E-4 40-41 5.195500574255622E-4 42-43 0.014639086912167313 44-45 3.05617680838566E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.05617680838566E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.222470723354264E-4 70-71 6.11235361677132E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.11235361677132E-5 82-83 0.001925391389282966 84-85 3.361794489224226E-4 86-87 0.0 88-89 0.0 90-91 0.01078830413360138 92-93 0.002383817910540815 94-95 0.0 96-97 0.0 98-99 0.0019559531573668226 100-101 0.019284475660913517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1636031.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.33188284203233 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98256247765136 39.76310557617852 2 13.129020337093515 13.74132708222875 3 4.444520932512368 6.977704461875925 4 2.024663421455917 4.238177958647175 5 1.0917796924256198 2.856744347666381 6 0.6597653228011248 2.071605694563846 7 0.4638077960268872 1.69903546700302 8 0.33359823782896475 1.3966259118699054 9 0.24732620721859677 1.1648741489935035 >10 1.4502175930002086 14.111514192542367 >50 0.11392627331867441 4.138289358634345 >100 0.05481268437032553 5.129018943750848 >500 0.0030580774031242685 1.0362878426777469 >1k 7.057101699517544E-4 0.7205265791390891 >5k 2.3523672331725144E-4 0.9551624342286337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8279 0.5060417559324977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7239 0.44247327831807587 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4673 0.2856302845117238 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1926 0.11772393065901564 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.11235361677132E-5 0.0 14-15 0.0 0.0 0.0 6.11235361677132E-5 0.0 16-17 0.0 0.0 0.0 9.168530425156981E-5 0.0 18-19 0.0 0.0 0.0 1.222470723354264E-4 0.0 20-21 0.0 0.0 0.0 1.52808840419283E-4 0.0 22-23 0.0 0.0 0.0 2.7505591275470945E-4 0.0 24-25 0.0 0.0 0.0 4.2786475317399243E-4 0.0 26-27 0.0 0.0 0.0 6.417971297609886E-4 0.0 28-29 0.0 0.0 0.0 0.001466964868025117 0.0 30-31 3.05617680838566E-5 0.0 0.0 0.002658873823295524 0.0 32-33 6.11235361677132E-5 0.0 0.0 0.004920444661500913 0.0 34-35 6.11235361677132E-5 0.0 0.0 0.006998644891203162 3.05617680838566E-5 36-37 6.11235361677132E-5 0.0 0.0 0.010146507003840392 6.11235361677132E-5 38-39 6.11235361677132E-5 0.0 0.0 0.015494816418515297 6.11235361677132E-5 40-41 6.11235361677132E-5 0.0 0.0 0.021515484731035046 6.11235361677132E-5 42-43 6.11235361677132E-5 0.0 0.0 0.0273222206669678 6.11235361677132E-5 44-45 6.11235361677132E-5 0.0 0.0 0.03144805935828844 6.11235361677132E-5 46-47 6.11235361677132E-5 0.0 0.0 0.03798827772823375 6.11235361677132E-5 48-49 6.11235361677132E-5 0.0 0.0 0.042664228245063815 6.11235361677132E-5 50-51 6.11235361677132E-5 0.0 0.0 0.04691231400871988 6.11235361677132E-5 52-53 6.11235361677132E-5 0.0 0.0 0.05794511228699212 6.11235361677132E-5 54-55 6.11235361677132E-5 0.0 0.0 0.07539588186287424 6.11235361677132E-5 56-57 6.11235361677132E-5 0.0 0.0 0.10174012595115862 6.11235361677132E-5 58-59 1.222470723354264E-4 0.0 0.0 0.13835312411561884 6.11235361677132E-5 60-61 1.222470723354264E-4 0.0 0.0 0.18602948232643512 6.11235361677132E-5 62-63 1.222470723354264E-4 0.0 0.0 0.21750810345280744 6.11235361677132E-5 64-65 1.222470723354264E-4 0.0 0.0 0.2566271666001439 6.11235361677132E-5 66-67 1.222470723354264E-4 0.0 0.0 0.3197677794613916 6.11235361677132E-5 68-69 1.222470723354264E-4 0.0 0.0 0.4050351124153515 6.11235361677132E-5 70-71 1.222470723354264E-4 0.0 0.0 0.491891657309672 6.11235361677132E-5 72-73 1.222470723354264E-4 0.0 0.0 0.5965657129968809 6.11235361677132E-5 74-75 1.222470723354264E-4 0.0 0.0 0.7068325722434354 6.11235361677132E-5 76-77 1.222470723354264E-4 0.0 0.0 0.8122706721327408 6.11235361677132E-5 78-79 1.222470723354264E-4 0.0 0.0 0.9152943923434214 6.11235361677132E-5 80-81 1.222470723354264E-4 0.0 0.0 1.014253397398949 6.11235361677132E-5 82-83 1.222470723354264E-4 0.0 0.0 1.1121427405715418 6.11235361677132E-5 84-85 1.222470723354264E-4 0.0 0.0 1.2753120203712522 6.11235361677132E-5 86-87 1.222470723354264E-4 0.0 0.0 1.492300573766634 6.11235361677132E-5 88-89 1.222470723354264E-4 0.0 0.0 1.7646976126980478 6.11235361677132E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5250 0.0 43.627293 1 GTATCAA 8325 0.0 35.67519 1 TATCAAC 10435 0.0 28.363506 2 ATCAACG 10485 0.0 28.228247 3 TCAACGC 10535 0.0 28.094275 4 GTATAGG 445 0.0 27.764118 1 CAACGCA 10755 0.0 27.519588 5 AACGCAG 10915 0.0 27.110388 6 GTACATG 7195 0.0 24.700817 1 TACATGG 7305 0.0 24.387953 2 ACATGGG 7255 0.0 23.508307 3 ACGCAGA 12595 0.0 23.3071 7 GAGTACT 7695 0.0 23.271837 12-13 CGCAGAG 12730 0.0 23.059933 8 GTACTTT 8225 0.0 21.742048 14-15 GCAGAGT 13505 0.0 21.384886 9 AGTACTT 8050 0.0 21.153936 12-13 GGACCGA 450 0.0 21.110031 6 TAAGGTG 805 0.0 20.655537 5 ACGTAAC 165 6.305019E-5 20.159725 1 >>END_MODULE