FastQCFastQC Report
Thu 26 May 2016
SRR937857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542172
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68890.4467076305366717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53840.3491179972143185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33930.2200143693440161No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21800.1413590701945049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46150.044.8216741
GTATCAA73650.035.7044941
ATCAACG90000.029.0436153
TATCAAC90850.028.9335212
TCAACGC90750.028.7530864
CAACGCA92700.028.0951465
AACGCAG95200.027.3957046
GTACATG70200.025.3340721
TACATGG70400.024.8471322
ACGCAGA107450.024.183997
CGCAGAG109300.023.818128
ACATGGG69850.023.7459473
GAGTACT62350.023.19676412-13
GCAGAGT113650.022.1937059
GTACTTT66050.021.93327714-15
GTATAGG5150.020.3135571
CATGGGA37550.020.2520394
AGTACTT65850.019.90810212-13
CAGAGTA113000.019.7561810-11
AGAGTAC103600.019.60017610-11
Produced by FastQC (version 0.11.5)