FastQCFastQC Report
Thu 26 May 2016
SRR937857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542172
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80860.5243254319232874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70290.45578573596200683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42840.27779002601525643No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18880.12242473602166294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46850.045.649091
GTATCAA75800.037.493951
TATCAAC98450.028.7554592
ATCAACG98500.028.6926383
TCAACGC99050.028.485364
CAACGCA100500.028.0271175
AACGCAG102450.027.5391276
GTACATG68350.024.8927971
ACGCAGA117900.023.7304157
ACATGGG67600.023.6092593
TACATGG69550.023.5619772
CGCAGAG118450.023.499928
GAGTACT71550.022.8045212-13
GCAGAGT124600.021.9969069
GTACTTT74850.021.63905314-15
AGTACTT75550.021.47138612-13
AGAGTAC110050.021.12839310-11
CATGGGG29650.020.9863244
ACTGCGT1604.9898015E-520.7817388
GTGTACG1856.523798E-620.5516871
Produced by FastQC (version 0.11.5)