##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937857_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1542172 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29411310800611 33.0 31.0 34.0 31.0 34.0 2 32.49333796749001 34.0 31.0 34.0 31.0 34.0 3 32.55907641949147 34.0 31.0 34.0 31.0 34.0 4 36.15366119991804 37.0 35.0 37.0 35.0 37.0 5 36.07689868574971 37.0 35.0 37.0 35.0 37.0 6 36.12971704842261 37.0 35.0 37.0 35.0 37.0 7 36.08755897526346 37.0 35.0 37.0 35.0 37.0 8 36.05241179323707 37.0 35.0 37.0 35.0 37.0 9 37.75267155673946 39.0 37.0 39.0 35.0 39.0 10-11 37.67040284741261 39.0 37.0 39.0 35.0 39.0 12-13 37.56403598301616 39.0 37.0 39.0 35.0 39.0 14-15 38.802090493148626 40.0 38.0 41.0 35.0 41.0 16-17 38.760128247692215 40.0 38.0 41.0 35.0 41.0 18-19 38.732947103176556 40.0 38.0 41.0 35.0 41.0 20-21 38.62703349561528 40.0 38.0 41.0 34.0 41.0 22-23 38.57769464106468 40.0 38.0 41.0 34.0 41.0 24-25 38.594491405627906 40.0 38.0 41.0 34.0 41.0 26-27 38.417527681737184 40.0 38.0 41.0 34.0 41.0 28-29 38.37695568328306 40.0 38.0 41.0 34.0 41.0 30-31 38.38058984341565 40.0 38.0 41.0 34.0 41.0 32-33 38.24031722790973 40.0 38.0 41.0 34.0 41.0 34-35 38.09110689339451 40.0 37.5 41.0 34.0 41.0 36-37 37.91462884814405 40.0 37.0 41.0 33.0 41.0 38-39 37.75864624698153 40.0 37.0 41.0 33.0 41.0 40-41 37.57340134563459 40.0 37.0 41.0 33.0 41.0 42-43 37.33973480260308 40.0 37.0 41.0 32.5 41.0 44-45 37.01330785411744 40.0 36.0 41.0 31.5 41.0 46-47 36.64395702943641 39.5 36.0 41.0 31.0 41.0 48-49 36.27533666802406 39.0 36.0 41.0 30.0 41.0 50-51 36.008452040369036 39.0 35.5 41.0 29.5 41.0 52-53 35.73272468959364 39.0 35.0 40.0 28.5 41.0 54-55 35.48936921432888 39.0 35.0 40.0 28.0 41.0 56-57 35.20231757547148 38.0 35.0 40.0 27.0 41.0 58-59 34.930677317445785 38.0 34.0 40.0 26.5 41.0 60-61 34.57330472865543 38.0 34.0 40.0 26.0 41.0 62-63 34.15917485209172 37.0 34.0 40.0 25.5 41.0 64-65 33.75916694117129 37.0 33.0 39.5 24.5 41.0 66-67 33.32606868753939 36.0 33.0 39.0 23.0 40.0 68-69 32.8581957784216 35.5 32.0 39.0 22.0 40.0 70-71 32.41757923240728 35.0 32.0 38.0 21.5 40.0 72-73 31.940919041455818 35.0 31.0 37.5 20.0 39.5 74-75 31.45189933418581 35.0 31.0 37.0 18.0 39.0 76-77 30.22393157183505 33.5 29.5 35.5 18.0 38.0 78-79 30.81611973242933 34.0 31.0 36.0 18.0 38.0 80-81 30.641724463937877 34.0 31.0 35.5 16.0 37.0 82-83 30.249776613762926 34.0 30.5 35.0 11.5 37.0 84-85 29.845751965409825 34.0 30.0 35.0 7.0 36.5 86-87 29.506046666649375 34.0 30.0 35.0 4.5 36.0 88-89 29.10546845617739 34.0 29.0 35.0 2.0 36.0 90-91 28.604596633838508 34.0 29.0 35.0 2.0 35.0 92-93 28.139216313096075 33.5 28.5 35.0 2.0 35.0 94-95 27.641100992625983 33.0 27.0 35.0 2.0 35.0 96-97 27.105603006668517 33.0 26.5 35.0 2.0 35.0 98-99 26.27885670340273 32.5 25.0 34.5 2.0 35.0 100-101 23.80993235514586 30.5 12.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28735229640711424 1101 2 0.26356828394185783 1101 3 0.2456095217171992 1101 4 0.1636495115319292 1101 5 0.19161276252921056 1101 6 0.19412058830317136 1101 7 0.1648294283701901 1101 8 0.1416089874795503 1101 9 0.18207659956236455 1101 10-11 0.24538033181286067 1101 12-13 0.30936610249484175 1101 14-15 0.4977646343095685 1101 16-17 0.5118251975654076 1101 18-19 0.4467571372427628 1101 20-21 0.5101067042686722 1101 22-23 0.4577921079544467 1101 24-25 0.3908673343511282 1101 26-27 0.2972761543513016 1101 28-29 0.4010398237144912 1101 30-31 0.44648777402644413 1101 32-33 0.36422102085608543 1101 34-35 0.38707034178951716 1101 36-37 0.34538240456961233 1101 38-39 0.29081108346503015 1101 40-41 0.3667192281260512 1101 42-43 0.39659307865830584 1101 44-45 0.186194543544012 1101 46-47 0.31837556366045305 1101 48-49 0.3134378238233353 1101 50-51 0.32900495976702615 1101 52-53 0.3443891308352178 1101 54-55 0.3394904670608625 1101 56-57 0.3945055372590147 1101 58-59 0.3986302697804902 1101 60-61 0.43508040683266813 1101 62-63 0.41270479151820894 1101 64-65 0.47430161003251925 1101 66-67 0.4382166000449885 1101 68-69 0.6316613444137076 1101 70-71 0.6536152952441299 1101 72-73 0.6527446286422958 1101 74-75 0.5040318369891104 1101 76-77 0.49391360663008044 1101 78-79 0.7501642883133961 1101 80-81 0.7033624571964125 1101 82-83 0.4359043929350115 1101 84-85 0.45860484338664165 1101 86-87 0.5020872743096305 1101 88-89 0.8219140817987771 1101 90-91 0.8319978989583383 1101 92-93 0.8649675125564364 1101 94-95 0.7777287531676613 1101 96-97 0.9312534031481547 1101 98-99 1.1466662091468258 1101 100-101 1.532218270623833 1102 1 0.11450992646696534 1102 2 0.09142287189384035 1102 3 0.11583404981862344 1102 4 0.1345982667113006 1102 5 0.08067179854578654 1102 6 0.14059259311954264 1102 7 0.11012177529089939 1102 8 0.11283326767928514 1102 9 0.19232202223474815 1102 10-11 0.18146065196018668 1102 12-13 0.19892147310786612 1102 14-15 0.13405248358534294 1102 16-17 0.2865367462426818 1102 18-19 0.30061802898055845 1102 20-21 0.2627254314705709 1102 22-23 0.19686973659771922 1102 24-25 0.12353617310878207 1102 26-27 0.08222498035972592 1102 28-29 0.18575420555392697 1102 30-31 0.252693815744756 1102 32-33 0.24884651223264598 1102 34-35 0.24776110213941394 1102 36-37 0.3737600650859534 1102 38-39 0.3190991452279661 1102 40-41 0.4220974552748302 1102 42-43 0.4357438382999135 1102 44-45 0.41732072550385624 1102 46-47 0.16367393105075223 1102 48-49 0.3078816386761858 1102 50-51 0.1683056999306345 1102 52-53 0.3087720327015617 1102 54-55 0.2783402032226334 1102 56-57 0.39498239386559675 1102 58-59 0.4462668813185431 1102 60-61 0.3674025373706229 1102 62-63 0.25353685583099406 1102 64-65 0.3390216581962733 1102 66-67 0.3415521172741549 1102 68-69 0.2946820264974974 1102 70-71 0.2782669933706998 1102 72-73 0.23539682257224825 1102 74-75 0.28929691472400165 1102 76-77 0.21373777350333256 1102 78-79 0.07258557952704692 1102 80-81 0.18632612189167475 1102 82-83 0.4100284146086999 1102 84-85 0.45198126439454356 1102 86-87 0.35502083665690165 1102 88-89 0.5681411951606812 1102 90-91 0.5237827911211035 1102 92-93 0.7161006003490478 1102 94-95 0.8904066226297118 1102 96-97 0.8351638752237989 1102 98-99 0.8823964566943694 1102 100-101 1.319389889421867 1103 1 0.19663006156723384 1103 2 0.31235396037459395 1103 3 0.25524007664186854 1103 4 0.11922979770044861 1103 5 0.18093973656206686 1103 6 0.21552341970600253 1103 7 0.20291255351213522 1103 8 0.1473792956027964 1103 9 0.23602466527513855 1103 10-11 0.23975537912908607 1103 12-13 0.34611188188767983 1103 14-15 0.34281497877167766 1103 16-17 0.41927163560008296 1103 18-19 0.37754599332573946 1103 20-21 0.33392871956127124 1103 22-23 0.35243553289198815 1103 24-25 0.20923662801454412 1103 26-27 0.21568027275541368 1103 28-29 0.1317999115334061 1103 30-31 0.23378280014500064 1103 32-33 0.28883227076145346 1103 34-35 0.22195753991200462 1103 36-37 0.11344584616246323 1103 38-39 0.09402404432505307 1103 40-41 0.19407216930252247 1103 42-43 0.2637480487544579 1103 44-45 0.23732786173615494 1103 46-47 0.19895547129918612 1103 48-49 0.06536983987300005 1103 50-51 0.12579843391343815 1103 52-53 0.055302901748987665 1103 54-55 0.022025102536666452 1103 56-57 -0.05123183877247328 1103 58-59 -0.05285697376557863 1103 60-61 0.13775358421173678 1103 62-63 -0.020407101887805368 1103 64-65 0.09540843113934017 1103 66-67 0.17910500946280905 1103 68-69 0.1746437806314347 1103 70-71 0.08027741955331891 1103 72-73 0.18309893252601483 1103 74-75 0.08912230560899559 1103 76-77 0.07728290029349338 1103 78-79 0.22516012448570066 1103 80-81 0.3979733285719931 1103 82-83 0.31574995278057116 1103 84-85 0.311832945438276 1103 86-87 0.278602907589967 1103 88-89 0.3567959805042058 1103 90-91 0.4137800172110495 1103 92-93 0.5033099306047326 1103 94-95 0.48652919226221414 1103 96-97 0.647283382707549 1103 98-99 0.7679248206995517 1103 100-101 1.0416288997403456 1104 1 0.1844279627509593 1104 2 0.11598354353174045 1104 3 0.02336058491985682 1104 4 0.10513588990575329 1104 5 0.15766270147400974 1104 6 0.1203410607441171 1104 7 0.141878567281303 1104 8 0.19240775795409348 1104 9 0.19197596374612402 1104 10-11 0.22174565245794042 1104 12-13 0.13196389608615533 1104 14-15 0.250079258083197 1104 16-17 0.2416795072205531 1104 18-19 0.3158754159071364 1104 20-21 0.20963152636644367 1104 22-23 0.20705712963956557 1104 24-25 0.3193270589711119 1104 26-27 0.08761258956962337 1104 28-29 0.2353258989176581 1104 30-31 0.3993775799130077 1104 32-33 0.34407659372231336 1104 34-35 0.2816985366608762 1104 36-37 0.2332555781769159 1104 38-39 0.32457208392821 1104 40-41 0.2382740304335158 1104 42-43 0.2998732167693845 1104 44-45 0.2540024512453911 1104 46-47 0.3641934928364208 1104 48-49 0.47746451968421155 1104 50-51 0.5132146106996984 1104 52-53 0.38388805710579277 1104 54-55 0.38507554432727176 1104 56-57 0.2632075878716762 1104 58-59 0.2717135574978684 1104 60-61 0.19799505587052124 1104 62-63 0.21584492373430209 1104 64-65 0.3122400495457569 1104 66-67 0.3096151852485747 1104 68-69 0.43043413484698334 1104 70-71 0.4172402899807466 1104 72-73 0.2002813670312804 1104 74-75 0.5781111666450975 1104 76-77 0.38780282301813784 1104 78-79 0.47053861986419676 1104 80-81 0.43820455581109385 1104 82-83 0.3908363900717333 1104 84-85 0.41008448463469804 1104 86-87 0.5293703178061975 1104 88-89 0.5188088984226376 1104 90-91 0.5458641670117323 1104 92-93 0.6713073584446825 1104 94-95 0.7416322859479862 1104 96-97 0.9612310864190299 1104 98-99 1.0805267927503444 1104 100-101 1.6864607650446537 1105 1 0.1359243172279747 1105 2 0.11811754922354112 1105 3 0.20660311884281413 1105 4 0.12950514744329666 1105 5 0.043219008786522295 1105 6 0.05483319597740888 1105 7 0.08732885257213496 1105 8 0.08846144498565423 1105 9 0.07687138513517056 1105 10-11 0.11005396947465584 1105 12-13 0.11156219628082908 1105 14-15 0.12105543535982122 1105 16-17 0.2363730809317559 1105 18-19 0.23487681111074465 1105 20-21 0.26961266953653507 1105 22-23 0.19983895488428516 1105 24-25 0.21101519929193557 1105 26-27 0.09140301417901497 1105 28-29 0.13478435363295915 1105 30-31 0.1417929469961834 1105 32-33 0.18303764069117534 1105 34-35 0.17444078722689227 1105 36-37 -0.013266082233094778 1105 38-39 0.05290929158147861 1105 40-41 0.07132353970189342 1105 42-43 0.16956742109692868 1105 44-45 0.09881926140296571 1105 46-47 0.029340140564045214 1105 48-49 -0.058814698172156454 1105 50-51 -0.1236140001113668 1105 52-53 -0.09031721215617239 1105 54-55 -0.0274725436720189 1105 56-57 -0.023892131910450587 1105 58-59 0.005132411749258381 1105 60-61 -0.06216221143624523 1105 62-63 -0.15697713763560017 1105 64-65 -0.14778139701525816 1105 66-67 -0.03563548895036206 1105 68-69 -0.006817857760502477 1105 70-71 -0.10470936273181763 1105 72-73 -0.14869947288241292 1105 74-75 0.08142876913707298 1105 76-77 0.0015360212968076326 1105 78-79 -0.021452552628151267 1105 80-81 0.15457462726412885 1105 82-83 0.046037517445583376 1105 84-85 0.199117533975798 1105 86-87 0.17502223216121138 1105 88-89 0.1937071169454896 1105 90-91 0.2378899253068596 1105 92-93 0.40630359436582 1105 94-95 0.1570896173253793 1105 96-97 0.31678659214811233 1105 98-99 0.6291829072904527 1105 100-101 0.8146036868106705 1106 1 -0.42441240947523795 1106 2 -0.34283694481631244 1106 3 -0.37545490405310744 1106 4 -0.26054283207218276 1106 5 -0.2935132878909599 1106 6 -0.22619687201429173 1106 7 -0.23833583773625122 1106 8 -0.301827053603553 1106 9 -0.5978020185515476 1106 10-11 -0.5529623385886353 1106 12-13 -0.7179915072157073 1106 14-15 -0.6247096887529935 1106 16-17 -0.45482746349902214 1106 18-19 -0.406688240908494 1106 20-21 -0.5522789166463582 1106 22-23 -0.6609707305142791 1106 24-25 -0.6844034406255304 1106 26-27 -0.6539143218391743 1106 28-29 -0.8018873471538583 1106 30-31 -0.765048173685976 1106 32-33 -0.7368434049142252 1106 34-35 -1.1755113575568927 1106 36-37 -0.8727081649915434 1106 38-39 -0.6884512881502829 1106 40-41 -0.7253722751419218 1106 42-43 -1.0711940361876273 1106 44-45 -0.9279231035148143 1106 46-47 -0.7656627183190068 1106 48-49 -0.6690038195006593 1106 50-51 -1.06720885909386 1106 52-53 -1.0168013453552547 1106 54-55 -1.1560744255628563 1106 56-57 -1.4061760687167038 1106 58-59 -1.2443949403035433 1106 60-61 -1.4340932626351162 1106 62-63 -1.4766276081083092 1106 64-65 -1.7391904534595497 1106 66-67 -1.7816200012622012 1106 68-69 -1.7160790850914296 1106 70-71 -1.7434248541489588 1106 72-73 -1.7530443536172697 1106 74-75 -1.7657253992387112 1106 76-77 -1.7945317715211786 1106 78-79 -1.6509981766725979 1106 80-81 -1.695087589488928 1106 82-83 -1.8202860832554641 1106 84-85 -1.6974549138495831 1106 86-87 -1.3827119787249167 1106 88-89 -1.7392250405168141 1106 90-91 -2.1146734362424056 1106 92-93 -2.2782537759589694 1106 94-95 -2.135875355142332 1106 96-97 -2.3204879600637938 1106 98-99 -2.4170172642425314 1106 100-101 -1.8021934940820472 1107 1 0.23000526120749498 1107 2 0.22269183697753192 1107 3 0.1686357442544093 1107 4 0.13877826182722686 1107 5 0.1799520018393892 1107 6 0.1451647650099943 1107 7 0.09296077729530339 1107 8 0.12477459107038413 1107 9 0.16457128418320366 1107 10-11 0.19853412344999555 1107 12-13 0.21252580010882838 1107 14-15 0.24625034184559524 1107 16-17 0.16466479123420186 1107 18-19 0.3243215123904193 1107 20-21 0.2761012707699706 1107 22-23 0.3687098967567124 1107 24-25 0.28449596652689024 1107 26-27 0.28321992945169683 1107 28-29 0.23166113247896902 1107 30-31 0.13558770315471946 1107 32-33 0.10082154058204651 1107 34-35 0.06572023548192618 1107 36-37 -0.04103384867867277 1107 38-39 0.008890459793875038 1107 40-41 0.05812839420212157 1107 42-43 0.17906413937175358 1107 44-45 0.1738841254008534 1107 46-47 -0.29289067777587974 1107 48-49 -0.2937934542300553 1107 50-51 -0.13290975792728688 1107 52-53 -0.06059078884350555 1107 54-55 -0.036673010378315496 1107 56-57 -0.026050805760114315 1107 58-59 -0.00582638034943983 1107 60-61 -0.11256428960011533 1107 62-63 0.07596370954325238 1107 64-65 0.07349839718111184 1107 66-67 0.12908950342320225 1107 68-69 0.1028011979454746 1107 70-71 0.034104310127197834 1107 72-73 -0.031061182838918455 1107 74-75 0.27548706968460124 1107 76-77 0.18507235928776922 1107 78-79 0.237971989707205 1107 80-81 0.4435479048694617 1107 82-83 0.5506835094008729 1107 84-85 0.6332871018321917 1107 86-87 0.7719112761995426 1107 88-89 0.7052911193607905 1107 90-91 0.837329814857231 1107 92-93 0.8558971133123983 1107 94-95 0.8920601965883996 1107 96-97 0.9723070294179408 1107 98-99 1.085535805178008 1107 100-101 1.6196940561187567 1108 1 0.19433759052476063 1108 2 0.12024785050532927 1108 3 0.14183049694939598 1108 4 0.07299935673316327 1108 5 0.037595734665430314 1108 6 0.15385743040842925 1108 7 0.16097493920610617 1108 8 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0.028021583303257103 2316 62-63 0.1185006392690724 2316 64-65 0.1130559352920315 2316 66-67 0.30342421250173857 2316 68-69 0.2456924862822163 2316 70-71 0.18416892846212107 2316 72-73 0.08544359058001305 2316 74-75 0.1428207643247532 2316 76-77 0.1779101464224695 2316 78-79 0.10265563779435993 2316 80-81 0.18282612501648288 2316 82-83 0.24310162476892216 2316 84-85 0.13538685030706077 2316 86-87 0.303627066697171 2316 88-89 0.46950923750812024 2316 90-91 0.536530770930657 2316 92-93 0.6534277201688887 2316 94-95 0.48688100230260645 2316 96-97 0.7341599775426175 2316 98-99 0.6260952748907691 2316 100-101 0.9692597931151816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 20.0 5 25.0 6 76.0 7 185.0 8 417.0 9 816.0 10 1364.0 11 2198.0 12 3421.0 13 5072.0 14 6709.0 15 9162.0 16 12721.0 17 17438.0 18 15727.0 19 10049.0 20 8340.0 21 8285.0 22 8310.0 23 9009.0 24 10187.0 25 11784.0 26 14279.0 27 17458.0 28 22011.0 29 27115.0 30 34208.0 31 43230.0 32 55563.0 33 73924.0 34 103136.0 35 149822.0 36 233979.0 37 350156.0 38 256941.0 39 19025.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04597656412172 19.571630962759993 15.363383256528419 22.01900921658986 2 15.616416327102295 20.413676295510488 40.35542079612391 23.614486581263307 3 19.62803111455791 24.62384221734022 29.896081630323984 25.852045037777888 4 13.244056592945919 17.152767105593348 36.37540843395447 33.227767867506266 5 13.58891185984317 37.68199869783223 33.97925091631627 14.749838526008338 6 31.94351862178797 36.532047009023636 16.89882840565125 14.625605963537142 7 28.68966626290712 33.165301924817726 20.074284839823314 18.07074697245184 8 26.63593944125558 35.57514985358313 18.949961482895553 18.83894922226574 9 26.586269235856957 17.093877985075594 20.152162015650653 36.16769076341679 10-11 25.198421447153756 27.151284033168803 26.50459870883403 21.145695810843407 12-13 26.058825512973982 24.401849183508574 27.605691251979465 21.933634051537982 14-15 22.76934738796969 25.801531865446915 26.165499049392675 25.263621697190715 16-17 21.78790692607569 29.18841089061402 26.924363819340513 22.09931836396978 18-19 21.81073187685939 27.128588769605464 29.94879948540111 21.111879868134036 20-21 22.362054001162647 26.102074965122206 30.47736939592607 21.058501637789075 22-23 22.262090842743095 25.9573899660117 30.288522797901756 21.49199639334344 24-25 22.04861066080826 26.344013508220872 30.36794858161087 21.239427249359995 26-27 21.63838477764528 26.66411828138972 30.270018603860965 21.427478337104038 28-29 21.463659303886754 26.900457381374103 30.322079877521524 21.313803437217622 30-31 21.57481239110319 26.59727747441756 30.240680183974412 21.58722995050484 32-33 21.593181564702252 26.59550945030775 30.198025901131654 21.613283083858352 34-35 22.079735593695126 26.597389915002996 29.961249458555855 21.361625032746023 36-37 22.10053911187252 26.35277265871508 29.926544872601262 21.620143356811134 38-39 21.71742192180898 26.618788306362713 30.011730209081737 21.65205956274657 40-41 21.994685403924862 26.707823012797565 29.85362165470947 21.443869928568095 42-43 21.88942983865659 26.733526371652488 29.61732947516126 21.759714314529663 44-45 21.94761673795141 26.912497438677395 29.59562876255048 21.544257060820712 46-47 22.297253484047175 27.203904622830656 28.836245243721194 21.662596649400975 48-49 22.28973162526618 27.326394202462502 28.606666441875483 21.77720773039583 50-51 22.395945458742606 27.47008764262352 28.492087782685722 21.641879115948157 52-53 22.56062312135842 27.612752537574455 28.18104212145018 21.645582219616948 54-55 22.258087943497873 27.751022583732553 28.27272184944351 21.718167623326064 56-57 22.293816772707583 27.86744928581248 28.2639031184589 21.57483082302104 58-59 22.434235610554467 27.323930145275625 28.306862010203794 21.93497223396612 60-61 22.16733931104961 27.52854415720166 28.732333358406198 21.57178317334253 62-63 22.13102688934827 27.6346931470679 28.789330891755267 21.444949071828564 64-65 22.06530789042986 27.515089108089107 28.629102330998098 21.790500670482928 66-67 21.95254485232516 27.773426051050077 28.349593949313046 21.924435147311712 68-69 22.10735897163222 27.652946623333847 28.47866515537826 21.76102924965568 70-71 22.146103028715345 27.732671842051342 28.226488355384483 21.89473677384883 72-73 22.2351657272989 27.67907859823677 28.102312841887933 21.98344283257639 74-75 22.13316672848424 28.210212609229064 27.848093468173456 21.808527194113235 76-77 22.247550856843464 27.681121171957475 28.132011215350815 21.939316755848246 78-79 22.257698881836784 27.751087427342735 28.093623798123684 21.897589892696796 80-81 22.259514502921853 28.168874807738696 27.826889607644283 21.744721081695168 82-83 22.260281967051537 28.32652256631258 27.639631531235935 21.77356393539995 84-85 22.175268137042092 28.213227024154623 27.60936953387389 22.00213530492939 86-87 22.095784387214916 28.162909195602047 27.763051073421124 21.978255343761916 88-89 22.197102528122674 28.186090786241742 27.54527380862835 22.071532877007233 90-91 22.26290898655802 28.13127128935034 27.61625748420474 21.9895622398869 92-93 22.286462041659046 27.76975860785481 27.958587363776783 21.98519198670936 94-95 22.142860848206297 27.810062690802322 27.926651501907703 22.12042495908368 96-97 22.18860801518897 27.798650215410476 27.7743662833977 22.23837548600286 98-99 21.966203994450655 27.9927335897046 27.570242992884353 22.470819422960393 100-101 21.77459077126415 28.086543353553328 27.92006643512086 22.218799440061662 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 599.0 1 396.0 2 261.5 3 336.5 4 294.0 5 282.0 6 485.0 7 1029.5 8 1725.5 9 2168.5 10 2124.5 11 2052.0 12 2220.0 13 2425.0 14 2638.0 15 2757.5 16 2852.0 17 2925.5 18 3103.5 19 3277.0 20 3445.5 21 3826.5 22 4344.5 23 5078.5 24 6138.0 25 7506.5 26 9495.5 27 12001.5 28 14155.0 29 16792.0 30 19801.0 31 22046.0 32 24855.5 33 27992.0 34 30860.5 35 33423.5 36 37354.0 37 41250.5 38 43630.0 39 45260.5 40 45868.5 41 48581.5 42 51459.5 43 53352.5 44 56190.5 45 58407.5 46 58624.0 47 59918.5 48 62266.5 49 60482.5 50 57231.5 51 54178.0 52 51101.5 53 49569.5 54 48882.5 55 45862.0 56 40370.5 57 35726.5 58 32433.0 59 28653.5 60 23400.5 61 18773.0 62 15159.0 63 11420.5 64 8216.5 65 6118.0 66 4447.0 67 3395.0 68 2539.5 69 1718.5 70 1248.0 71 892.5 72 691.0 73 635.5 74 498.0 75 339.5 76 215.0 77 131.5 78 98.0 79 71.0 80 42.5 81 17.5 82 12.0 83 9.0 84 5.0 85 4.0 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03916554054930319 2 0.0 3 0.0 4 6.484361018096554E-5 5 0.00829998210316359 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005576550475563037 14-15 0.0 16-17 0.0 18-19 0.0 20-21 9.726541527144833E-5 22-23 0.0015238248392526904 24-25 0.0 26-27 0.0012320285934383454 28-29 0.0015238248392526904 30-31 6.484361018096554E-5 32-33 0.0 34-35 0.0 36-37 3.890616610857933E-4 38-39 0.0 40-41 2.593744407238622E-4 42-43 0.021819874825894908 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.242180509048277E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.890616610857933E-4 84-85 2.269526356333794E-4 86-87 0.0 88-89 0.0 90-91 0.011315209976578488 92-93 0.0022046827461528287 94-95 0.0 96-97 0.0 98-99 4.863270763572416E-4 100-101 0.014038641604179043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1542172.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.971276844752815 #Duplication Level Percentage of deduplicated Percentage of total 1 76.32399148520713 40.42979282859464 2 13.07826799946784 13.85545109699365 3 4.327530288684148 6.877044149278231 4 1.94681775807357 4.125016897167844 5 1.0742727777551124 2.8452800358623835 6 0.6732225462288163 2.1396874724649617 7 0.4384341111885331 1.6257090277365647 8 0.3165686605019109 1.341523692465543 9 0.23921242237494858 1.140424870529461 >10 1.4159297622802447 13.979343204021472 >50 0.10942500965517989 4.00328530381499 >100 0.05238313809816057 4.927084984164522 >500 0.0029580303633107404 1.0008614967906286 >1k 7.395075908276851E-4 0.7226703136317983 >5k 2.4650253027589503E-4 0.9868246264833889 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8086 0.5243254319232874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7029 0.45578573596200683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4284 0.27779002601525643 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1888 0.12242473602166294 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.484361018096554E-5 0.0 20-21 0.0 0.0 0.0 6.484361018096554E-5 0.0 22-23 0.0 0.0 0.0 6.484361018096554E-5 6.484361018096554E-5 24-25 0.0 0.0 0.0 6.484361018096554E-5 1.296872203619311E-4 26-27 0.0 0.0 0.0 3.566398559953105E-4 1.296872203619311E-4 28-29 0.0 0.0 0.0 0.0013617158138002766 1.296872203619311E-4 30-31 0.0 0.0 0.0 0.0029828060683244153 1.296872203619311E-4 32-33 0.0 0.0 0.0 0.005122645204296278 1.296872203619311E-4 34-35 0.0 0.0 0.0 0.007100375314815728 1.296872203619311E-4 36-37 0.0 0.0 0.0 0.010893726510402213 1.296872203619311E-4 38-39 0.0 0.0 0.0 0.016989025867412973 1.296872203619311E-4 40-41 0.0 0.0 0.0 0.021917140241166352 1.296872203619311E-4 42-43 0.0 0.0 0.0 0.027072207250553117 1.296872203619311E-4 44-45 0.0 0.0 0.0 0.031384307327587325 1.296872203619311E-4 46-47 0.0 0.0 0.0 0.037479606684598085 1.296872203619311E-4 48-49 0.0 0.0 0.0 0.04318584438052306 1.296872203619311E-4 50-51 0.0 0.0 0.0 0.0481788023644574 1.296872203619311E-4 52-53 0.0 0.0 0.0 0.05998033941739313 1.296872203619311E-4 54-55 0.0 0.0 0.0 0.07855803373423975 1.296872203619311E-4 56-57 0.0 0.0 0.0 0.10692711318841219 1.296872203619311E-4 58-59 0.0 0.0 0.0 0.1447633597290056 1.296872203619311E-4 60-61 0.0 0.0 0.0 0.19433629971235375 1.296872203619311E-4 62-63 0.0 0.0 0.0 0.2266284175824746 1.296872203619311E-4 64-65 0.0 0.0 0.0 0.2649509911994252 1.296872203619311E-4 66-67 0.0 0.0 0.0 0.33316646910980097 1.296872203619311E-4 68-69 0.0 0.0 0.0 0.4219697932526333 1.296872203619311E-4 70-71 0.0 0.0 0.0 0.5110973354463705 1.296872203619311E-4 72-73 3.242180509048277E-5 0.0 0.0 0.6185756193213209 1.296872203619311E-4 74-75 6.484361018096554E-5 0.0 0.0 0.7318249845023772 1.9453083054289663E-4 76-77 6.484361018096554E-5 0.0 0.0 0.8386548322755178 1.9453083054289663E-4 78-79 6.484361018096554E-5 0.0 0.0 0.947851471820264 1.9453083054289663E-4 80-81 6.484361018096554E-5 0.0 0.0 1.0505637503469134 1.9453083054289663E-4 82-83 6.484361018096554E-5 0.0 0.0 1.1552861807891728 1.9453083054289663E-4 84-85 6.484361018096554E-5 0.0 0.0 1.3304287718879606 1.9453083054289663E-4 86-87 6.484361018096554E-5 0.0 0.0 1.5687614611080996 1.9453083054289663E-4 88-89 6.484361018096554E-5 0.0 0.0 1.8587420858373773 1.9453083054289663E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4685 0.0 45.64909 1 GTATCAA 7580 0.0 37.49395 1 TATCAAC 9845 0.0 28.755459 2 ATCAACG 9850 0.0 28.692638 3 TCAACGC 9905 0.0 28.48536 4 CAACGCA 10050 0.0 28.027117 5 AACGCAG 10245 0.0 27.539127 6 GTACATG 6835 0.0 24.892797 1 ACGCAGA 11790 0.0 23.730415 7 ACATGGG 6760 0.0 23.609259 3 TACATGG 6955 0.0 23.561977 2 CGCAGAG 11845 0.0 23.49992 8 GAGTACT 7155 0.0 22.80452 12-13 GCAGAGT 12460 0.0 21.996906 9 GTACTTT 7485 0.0 21.639053 14-15 AGTACTT 7555 0.0 21.471386 12-13 AGAGTAC 11005 0.0 21.128393 10-11 CATGGGG 2965 0.0 20.986324 4 ACTGCGT 160 4.9898015E-5 20.781738 8 GTGTACG 185 6.523798E-6 20.551687 1 >>END_MODULE