FastQCFastQC Report
Thu 26 May 2016
SRR937856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371494
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61930.4515513739032034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50020.36471176687612195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31300.22821827875295114No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19160.1397016683995701No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.10069311276607845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.043.8310171
GTATCAA65050.039.7791
ATCAACG81250.031.831373
TATCAAC81250.031.7143422
TCAACGC81600.031.6377334
CAACGCA82900.031.1978155
AACGCAG84100.030.7844246
ACGCAGA93850.027.4344067
CGCAGAG94050.027.2750458
GTACATG60900.025.5406931
GCAGAGT100050.024.972819
TACATGG60950.024.7266542
ACATGGG61050.023.2844163
GGACCGA2452.9649527E-1023.2672846
GAGTACT56850.023.18332912-13
GTACTTT59200.021.98305114-15
TTAGTCG550.002880002821.588838-39
AGTACTT60250.021.48091112-13
AGAGTAC92350.021.18943810-11
CATGGGA33250.020.7334754
Produced by FastQC (version 0.11.5)