##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937856_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1371494 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75859901683857 33.0 31.0 34.0 30.0 34.0 2 31.863141216804447 33.0 31.0 34.0 30.0 34.0 3 31.90944182037982 33.0 31.0 34.0 30.0 34.0 4 35.50296902501943 37.0 35.0 37.0 33.0 37.0 5 35.50423625622861 37.0 35.0 37.0 33.0 37.0 6 35.56833351075542 37.0 35.0 37.0 33.0 37.0 7 35.49749324459312 37.0 35.0 37.0 33.0 37.0 8 35.4932307396168 37.0 35.0 37.0 33.0 37.0 9 37.06287522949426 39.0 37.0 39.0 33.0 39.0 10-11 36.985403873440205 39.0 37.0 39.0 33.0 39.0 12-13 36.88084526800701 39.0 37.0 39.0 33.0 39.0 14-15 38.012759078785614 40.0 38.0 41.0 33.0 41.0 16-17 37.912076902997754 40.0 38.0 41.0 33.0 41.0 18-19 37.829073987928496 40.0 38.0 41.0 33.0 41.0 20-21 37.83498943487904 40.0 38.0 41.0 33.0 41.0 22-23 37.84471095024841 40.0 38.0 41.0 33.0 41.0 24-25 37.76911601509011 40.0 38.0 41.0 33.0 41.0 26-27 37.65307139513553 40.0 37.5 41.0 32.5 41.0 28-29 37.500656218692896 40.0 37.0 41.0 32.0 41.0 30-31 37.24636710040292 40.0 37.0 41.0 31.5 41.0 32-33 37.0902235080868 39.5 36.0 41.0 31.0 41.0 34-35 37.04777162714529 40.0 36.0 41.0 31.0 41.0 36-37 36.87726778243288 40.0 36.0 41.0 31.0 41.0 38-39 36.69404459662237 39.0 36.0 41.0 30.0 41.0 40-41 36.44750432739771 39.0 35.5 41.0 30.0 41.0 42-43 36.163895722474905 39.0 35.0 41.0 30.0 41.0 44-45 35.891387421308444 39.0 35.0 41.0 28.5 41.0 46-47 35.49090918370769 39.0 35.0 40.0 26.5 41.0 48-49 35.09719546713292 39.0 35.0 40.0 25.5 41.0 50-51 34.22369729652481 37.5 33.5 39.5 23.5 40.5 52-53 34.319250758661724 38.0 34.0 39.5 24.0 40.5 54-55 34.55523575021108 38.0 34.0 40.0 24.0 41.0 56-57 34.29253974133317 38.0 34.0 40.0 23.0 41.0 58-59 33.988426125086946 38.0 34.0 40.0 22.0 41.0 60-61 33.611614414645636 37.5 33.0 40.0 20.5 41.0 62-63 33.21697397145011 37.0 33.0 40.0 18.5 41.0 64-65 32.76781013989124 36.5 32.0 39.0 17.0 41.0 66-67 32.24046149673276 36.0 31.5 39.0 12.0 40.5 68-69 31.74718482180746 35.0 31.0 39.0 8.0 40.0 70-71 31.247590948265177 35.0 30.5 38.0 7.0 40.0 72-73 30.73965726426802 35.0 30.0 37.5 2.0 39.5 74-75 30.173286576536242 34.5 29.5 37.0 2.0 39.0 76-77 29.61628742087096 34.0 29.0 36.0 2.0 39.0 78-79 29.0510753236981 34.0 28.5 36.0 2.0 38.0 80-81 28.481077569424293 34.0 27.5 35.0 2.0 37.0 82-83 27.90473162842856 33.5 26.0 35.0 2.0 37.0 84-85 27.325642328730567 33.0 26.0 35.0 2.0 36.0 86-87 26.715474511736836 32.5 25.0 35.0 2.0 36.0 88-89 26.132825225629862 32.0 24.0 35.0 2.0 35.0 90-91 25.520800309735222 32.0 21.0 34.0 2.0 35.0 92-93 24.80709139084823 31.0 18.5 34.0 2.0 35.0 94-95 23.994547187227944 31.0 9.0 34.0 2.0 35.0 96-97 23.065418441495186 31.0 2.0 34.0 2.0 35.0 98-99 21.83026174376264 30.0 2.0 34.0 2.0 35.0 100-101 18.616332627047584 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18849676093202916 1101 2 8.962803480869752E-4 1101 3 0.01958097619791843 1101 4 0.10356846127316288 1101 5 0.06804503621234659 1101 6 0.163793183009453 1101 7 0.09075976322822044 1101 8 0.08660365213832222 1101 9 0.35194947322866454 1101 10-11 0.3634337348302168 1101 12-13 0.34938786742066696 1101 14-15 0.4968546484504799 1101 16-17 0.46300687789729267 1101 18-19 0.5341597698418852 1101 20-21 0.41440855706127877 1101 22-23 0.35331399451812473 1101 24-25 0.3499492391707406 1101 26-27 0.396958922520227 1101 28-29 0.4003689455633719 1101 30-31 0.40608013177264723 1101 32-33 0.3760342739289584 1101 34-35 0.47892435778111775 1101 36-37 0.5537730060261836 1101 38-39 0.45949578092412935 1101 40-41 0.4472971175410194 1101 42-43 0.43221588473253547 1101 44-45 0.7639907155708414 1101 46-47 0.5506985429468685 1101 48-49 0.6596140262947614 1101 50-51 0.5974406569104573 1101 52-53 0.7564421527722516 1101 54-55 0.9754052337985257 1101 56-57 1.1042665142261825 1101 58-59 0.8534391346809755 1101 60-61 1.118255479402272 1101 62-63 1.1490522477323282 1101 64-65 1.0501052215456994 1101 66-67 1.1254930394260185 1101 68-69 1.339225948444792 1101 70-71 1.218331426876194 1101 72-73 1.4071314850207806 1101 74-75 1.418913675341532 1101 76-77 1.2239447469729043 1101 78-79 1.485519391363404 1101 80-81 1.5547584578786378 1101 82-83 1.6022518278311928 1101 84-85 1.8912354773824944 1101 86-87 2.0699855556903763 1101 88-89 1.9880242416511038 1101 90-91 2.0707913317236297 1101 92-93 2.117052583841339 1101 94-95 2.2574674880864087 1101 96-97 2.4466613595529907 1101 98-99 3.0027834995699187 1101 100-101 3.8172262522370914 1102 1 0.2002524307396314 1102 2 0.24752888039824583 1102 3 0.2216867767783306 1102 4 0.2858841253482609 1102 5 0.1623586580818639 1102 6 0.1865969163045662 1102 7 0.20758780593539683 1102 8 0.13088547650864513 1102 9 0.3492475815013947 1102 10-11 0.4840846430742758 1102 12-13 0.3935776581841637 1102 14-15 0.4789295920211174 1102 16-17 0.5599576768654373 1102 18-19 0.5994884037138348 1102 20-21 0.43069738583847084 1102 22-23 0.3442969403101088 1102 24-25 0.5276276186626987 1102 26-27 0.5574570236273146 1102 28-29 0.5773287463587593 1102 30-31 0.6018024724268614 1102 32-33 0.5580330826423676 1102 34-35 0.627532657376257 1102 36-37 0.6109690935902208 1102 38-39 0.7182593508427999 1102 40-41 0.45465823860439514 1102 42-43 0.6850084665103324 1102 44-45 0.6516371632079796 1102 46-47 0.7235804264336636 1102 48-49 0.755311511157359 1102 50-51 0.5728305121646571 1102 52-53 0.6826762092374494 1102 54-55 0.804545626475246 1102 56-57 0.8286587789246838 1102 58-59 0.9105581916835916 1102 60-61 0.8911429610403374 1102 62-63 0.972398105996092 1102 64-65 1.003722970820185 1102 66-67 0.850022570793584 1102 68-69 1.0817369656064812 1102 70-71 1.1277555786443152 1102 72-73 1.1251311088250127 1102 74-75 1.1542906324552185 1102 76-77 1.1727951106288117 1102 78-79 1.487352002162016 1102 80-81 1.6568875467683242 1102 82-83 1.7192133304645623 1102 84-85 1.9515123395515346 1102 86-87 2.1295655599897607 1102 88-89 2.1768716046979293 1102 90-91 2.322558780041497 1102 92-93 2.7079526769945765 1102 94-95 2.826402227078205 1102 96-97 2.8678320001606394 1102 98-99 3.107876921518251 1102 100-101 3.456109219525974 1103 1 0.265611632686678 1103 2 0.16867316480961492 1103 3 0.3367842862614516 1103 4 0.2686902355299736 1103 5 0.21271226501460916 1103 6 0.23152594636344048 1103 7 0.19052935299996676 1103 8 0.2832504479654503 1103 9 0.31239048821023374 1103 10-11 0.3764161256059637 1103 12-13 0.3651141008507395 1103 14-15 0.4770045621692063 1103 16-17 0.5178253930749506 1103 18-19 0.6880237983931465 1103 20-21 0.6527890182739213 1103 22-23 0.5480292663041411 1103 24-25 0.5671579418140809 1103 26-27 0.5565665388038568 1103 28-29 0.6000022501362778 1103 30-31 0.6709886540096832 1103 32-33 0.84513822325831 1103 34-35 0.7919543013060988 1103 36-37 0.7927015494413538 1103 38-39 0.7513343564986812 1103 40-41 0.8446160249063013 1103 42-43 0.8455762463295216 1103 44-45 0.8569901665081687 1103 46-47 0.8866910717726597 1103 48-49 1.0256417539596754 1103 50-51 0.859639162675613 1103 52-53 0.8007794537370359 1103 54-55 0.8141268982427903 1103 56-57 0.7997318448403519 1103 58-59 0.9043450880890873 1103 60-61 1.0824830658796358 1103 62-63 1.3412021222322892 1103 64-65 1.2419300705534724 1103 66-67 1.468110597665877 1103 68-69 1.5511464912324584 1103 70-71 1.5738740184520061 1103 72-73 1.5184078596383976 1103 74-75 1.491724778173129 1103 76-77 1.4938198352151204 1103 78-79 1.6460888477913613 1103 80-81 1.9009993395164138 1103 82-83 1.818483493059837 1103 84-85 1.9759176093144113 1103 86-87 2.0854818691267276 1103 88-89 2.1938800930904314 1103 90-91 2.2855959242406882 1103 92-93 2.282860686437619 1103 94-95 2.800075731870237 1103 96-97 2.784458931911608 1103 98-99 3.058159411406141 1103 100-101 3.6506749540960612 1104 1 0.1960163658181706 1104 2 0.1831482031349978 1104 3 0.11711151457825153 1104 4 0.11078645252636932 1104 5 0.2771971248867544 1104 6 0.144674648851705 1104 7 0.2036443206102163 1104 8 0.2758586664649698 1104 9 0.313054510176336 1104 10-11 0.36003869637448105 1104 12-13 0.4089198007640533 1104 14-15 0.4627072724890837 1104 16-17 0.5455349656668531 1104 18-19 0.5406840497393404 1104 20-21 0.644296583000056 1104 22-23 0.42284724742115287 1104 24-25 0.3666030244218348 1104 26-27 0.4452227590456417 1104 28-29 0.4428964678073797 1104 30-31 0.5466217167522842 1104 32-33 0.36129573524700476 1104 34-35 0.6342827124787505 1104 36-37 0.5670477760457899 1104 38-39 0.5786293957635138 1104 40-41 0.7986704021134798 1104 42-43 0.7859813424312208 1104 44-45 1.1201360933419195 1104 46-47 0.8308683891239141 1104 48-49 1.054107692408465 1104 50-51 0.9999789241401302 1104 52-53 0.9659495800079654 1104 54-55 1.0322047361360305 1104 56-57 1.114146245790053 1104 58-59 1.2357721161317343 1104 60-61 1.510645390426248 1104 62-63 1.4335540197124175 1104 64-65 1.3061827362493617 1104 66-67 1.4941921421254634 1104 68-69 1.6460386463787415 1104 70-71 1.494618940071792 1104 72-73 1.6105830760326931 1104 74-75 1.8347983458286414 1104 76-77 1.7943319434741838 1104 78-79 1.8921548912880048 1104 80-81 1.9444281909505747 1104 82-83 1.9112191781554273 1104 84-85 2.2655021792374193 1104 86-87 2.3819954335368614 1104 88-89 2.365274090844288 1104 90-91 2.613595018950292 1104 92-93 2.8046543784424536 1104 94-95 3.0672303443675126 1104 96-97 3.129954791916134 1104 98-99 3.252276411649543 1104 100-101 3.8766799545444357 1105 1 0.010346641773811172 1105 2 0.062094628290083165 1105 3 -0.04947726995468571 1105 4 -0.24897677845701338 1105 5 -0.24875576760071283 1105 6 -0.07178362355030998 1105 7 -0.06827556011467095 1105 8 -0.23239097564371747 1105 9 -0.13828106762846204 1105 10-11 -0.11894852069347195 1105 12-13 -0.03841987382423184 1105 14-15 -0.17960125291075002 1105 16-17 0.0015649119886589347 1105 18-19 -0.007809673143938767 1105 20-21 -0.09202548179641212 1105 22-23 -0.05526640698447949 1105 24-25 -0.04793242056185676 1105 26-27 -0.006123539005805867 1105 28-29 -0.0244048557857397 1105 30-31 0.22906024850730233 1105 32-33 0.14416071291591237 1105 34-35 0.08812655311981388 1105 36-37 -0.0027847352187180263 1105 38-39 0.06112158372737042 1105 40-41 -0.01776049242788247 1105 42-43 0.012798396749339247 1105 44-45 -0.09052719855445446 1105 46-47 0.0833769268382909 1105 48-49 0.06108987151026213 1105 50-51 0.003734583880607545 1105 52-53 -0.14142688426777994 1105 54-55 -0.059121869646972414 1105 56-57 0.05659666327493085 1105 58-59 0.03574110987990764 1105 60-61 0.1263011836475343 1105 62-63 0.21431535513629285 1105 64-65 0.24808862018043243 1105 66-67 0.5814461536967386 1105 68-69 0.43576733068011464 1105 70-71 0.5111905172344784 1105 72-73 0.29453151329560967 1105 74-75 0.5183045041980172 1105 76-77 0.6037075413340958 1105 78-79 0.8494184906080626 1105 80-81 0.9162996381508854 1105 82-83 1.1696403442104 1105 84-85 1.4155705139378156 1105 86-87 1.494766930655036 1105 88-89 1.4115632912128433 1105 90-91 1.2783465181355531 1105 92-93 1.6325446668885562 1105 94-95 1.7010597044292624 1105 96-97 1.7628822768693055 1105 98-99 1.8609079694168251 1105 100-101 2.28163177390773 1106 1 -0.1361129625291646 1106 2 -0.09816705595878261 1106 3 -0.043944893561228326 1106 4 -0.188258032928438 1106 5 -0.17017976307400318 1106 6 -0.008954363823733047 1106 7 -0.213009193060806 1106 8 -0.15725228006506597 1106 9 0.008974450927148325 1106 10-11 -0.4424705916639837 1106 12-13 -0.3121810442653228 1106 14-15 -0.4272309893728874 1106 16-17 -0.3941853611749693 1106 18-19 0.040721991072928176 1106 20-21 -0.01887088094049716 1106 22-23 -0.06594336498232423 1106 24-25 -0.006741215780195375 1106 26-27 -0.12240994456274024 1106 28-29 0.18789124707987526 1106 30-31 0.20867825844525356 1106 32-33 0.33916986714929465 1106 34-35 0.2531438047035053 1106 36-37 -0.054617939558120554 1106 38-39 -0.1263873591293958 1106 40-41 0.055141675970986626 1106 42-43 0.40041392219908545 1106 44-45 0.5810959787287402 1106 46-47 0.6097083556141314 1106 48-49 0.8396541690244703 1106 50-51 0.7496875346981469 1106 52-53 0.8713451411754605 1106 54-55 0.9313430125674813 1106 56-57 0.6449500557069996 1106 58-59 0.7065557716122939 1106 60-61 0.9702849174040509 1106 62-63 0.7512489471078823 1106 64-65 1.1723621350158098 1106 66-67 1.412513556821203 1106 68-69 0.9477284016116059 1106 70-71 1.1805861101946569 1106 72-73 1.1799292102660957 1106 74-75 1.3975153702567873 1106 76-77 1.7051166916651432 1106 78-79 1.5731866527371814 1106 80-81 1.5595755854433122 1106 82-83 1.5203885361273564 1106 84-85 1.6752564408080062 1106 86-87 1.7810972862880625 1106 88-89 1.7783732603843774 1106 90-91 2.0910745609832198 1106 92-93 1.9987318880340261 1106 94-95 1.9066702088714251 1106 96-97 2.1589829474209665 1106 98-99 2.5980221257965006 1106 100-101 2.853315681318268 1107 1 -0.009750275797888008 1107 2 -0.05642348454142976 1107 3 -0.02101582395817303 1107 4 -0.07416738088087271 1107 5 -0.061521204458841794 1107 6 0.0766376783999192 1107 7 0.05506211433304742 1107 8 -0.017613215331557797 1107 9 0.16956629679825141 1107 10-11 0.16980232660622363 1107 12-13 0.035424829032301375 1107 14-15 0.2955903808537812 1107 16-17 0.21571070941769932 1107 18-19 0.38425305797417053 1107 20-21 0.3472417115415567 1107 22-23 0.11964612465811086 1107 24-25 0.44927335750643493 1107 26-27 0.3456827652596033 1107 28-29 0.3364879153697231 1107 30-31 0.3883550817750816 1107 32-33 0.10695989950819751 1107 34-35 0.30130466849767146 1107 36-37 0.16929907792511045 1107 38-39 0.27352192599022374 1107 40-41 0.4297744966932555 1107 42-43 0.46098917627929126 1107 44-45 0.2760681752069587 1107 46-47 0.46426952728909754 1107 48-49 0.3354414249582476 1107 50-51 0.08460805118389914 1107 52-53 0.24522593161230333 1107 54-55 0.42533511526772116 1107 56-57 0.3599192872616399 1107 58-59 0.3799866201694826 1107 60-61 0.5559505918358099 1107 62-63 0.6219179006273095 1107 64-65 0.7881021485248709 1107 66-67 0.8877500634736251 1107 68-69 0.8505412501373897 1107 70-71 0.8216830926485983 1107 72-73 1.1784334946788455 1107 74-75 1.3426451396603483 1107 76-77 1.1937748772104513 1107 78-79 1.2973407659304996 1107 80-81 1.3609276691366254 1107 82-83 1.747349018212148 1107 84-85 1.7134764656269716 1107 86-87 2.088951093956805 1107 88-89 2.1113282877951924 1107 90-91 2.2808124892281434 1107 92-93 2.4801196293513144 1107 94-95 2.9112849847938875 1107 96-97 2.993755708609136 1107 98-99 3.1576485061549597 1107 100-101 3.3221766205118115 1108 1 0.10730727766101111 1108 2 0.15733022723716772 1108 3 0.15024678005181258 1108 4 0.07668824844601829 1108 5 0.27472584342162776 1108 6 0.2655547590807785 1108 7 0.30917482410445984 1108 8 0.17868015487056255 1108 9 0.28796591460532994 1108 10-11 0.28848766062832 1108 12-13 0.4295701366182527 1108 14-15 0.46971858767011554 1108 16-17 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-2.4929370699075157 2316 86-87 -2.66762225573763 2316 88-89 -2.613422024153074 2316 90-91 -2.7610908352860726 2316 92-93 -2.7910173465712838 2316 94-95 -2.5745194275266456 2316 96-97 -2.9289812210605533 2316 98-99 -2.950351607751461 2316 100-101 -2.8167331224566254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6044.0 3 1292.0 4 1194.0 5 1320.0 6 1589.0 7 1797.0 8 2186.0 9 2813.0 10 3427.0 11 4419.0 12 5718.0 13 7154.0 14 8523.0 15 10249.0 16 12857.0 17 16369.0 18 14672.0 19 10665.0 20 10600.0 21 11218.0 22 12215.0 23 13662.0 24 15293.0 25 17272.0 26 20068.0 27 23124.0 28 27086.0 29 32305.0 30 38503.0 31 47426.0 32 59327.0 33 77211.0 34 103034.0 35 141143.0 36 197524.0 37 246581.0 38 154088.0 39 11526.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.828707809102376 18.60862592225139 14.848722968998304 22.713943299647926 2 14.961153867246768 19.44946346635468 41.825055298612504 23.764327367786045 3 19.1543661951232 24.418517069532246 29.77846354100015 26.6486531943444 4 12.44222479772738 16.361469305381384 37.304372305926144 33.891933590965095 5 13.45036338203045 37.306344323394285 34.32645811655573 14.916834178019537 6 31.62117947402762 35.90751899531428 17.738215014025005 14.733086516633092 7 28.48072042163895 32.7611827983699 20.58061565482183 18.177481125169315 8 26.176952381230723 35.19393392963347 19.603379066562816 19.025734622572994 9 26.28242504482624 17.112517883003147 20.596908706446943 36.00814836572367 10-11 24.77289250159832 26.76269978993515 27.172381039364325 21.292026669102203 12-13 25.69959476186231 24.09946674577554 28.061462335358307 22.13947615700384 14-15 22.539011154812016 25.438991938754114 26.548226094822112 25.47377081161176 16-17 21.64017257899368 28.843237825060484 27.24851499290053 22.268074603045303 18-19 21.627713723721147 26.97650038992514 30.014738622313214 21.3810472640405 20-21 22.199141251522718 26.050432752981056 30.4067092692602 21.343716726236025 22-23 21.970220284851038 25.91951564583903 30.28532227020191 21.824941799108018 24-25 21.689975591563055 26.220622754858493 30.365381369955486 21.72402028362297 26-27 21.42167743368457 26.476050752327414 30.302311852389813 21.799959961598205 28-29 21.356672665537037 26.697274788450308 30.32498387027242 21.621068675740233 30-31 21.45638215280632 26.279143261634015 30.340813810110973 21.923660775448692 32-33 21.197475675944247 26.490095016527793 30.202089136083192 22.110340171444772 34-35 21.85302281809168 26.464500035437492 29.999298556853795 21.683178589617032 36-37 21.80393890190735 26.15214615908663 30.05915841267696 21.984756526329054 38-39 21.424874525761986 26.500823832745336 30.064544218323164 22.009757423169514 40-41 21.73446806210401 26.47904999519709 29.993246705623207 21.793235237075695 42-43 21.56384808979899 26.412168011484216 29.832266124112838 22.19171777460395 44-45 21.64830508474576 26.57250876680304 29.85615137346581 21.92303477498539 46-47 21.839059044951227 26.855737346792875 29.21031683841792 22.094886769837974 48-49 21.84061196634156 26.98623090345265 28.99215386548899 22.1810032647168 50-51 21.957468323724722 27.0908460560238 28.920093186682795 22.031592433568687 52-53 22.09411722581477 27.1618722896787 28.69359088137301 22.050419603133513 54-55 21.801325645214558 27.127140364669334 28.863250461024105 22.20828352909201 56-57 21.851693183548832 27.394362519251718 28.721064897243203 22.032879399956247 58-59 21.967329488397638 26.95039682394776 28.85801466061273 22.22425902704187 60-61 21.812406546954243 26.988655247651028 29.214727798259027 21.984210407135706 62-63 21.753517688231792 27.09838790197912 29.231186681337928 21.916907728451157 64-65 21.598675233528805 27.074366908303602 29.068784715221955 22.258173142945633 66-67 21.596109014503543 27.25647465815638 28.816068247833016 22.33134807950706 68-69 21.627770146712454 27.133130295459186 29.03997951847227 22.19912003935609 70-71 21.687669739942702 27.099844704844244 28.83179474742989 22.380690807783164 72-73 21.74548913224123 27.179605935764673 28.733457838238834 22.341447093755264 74-75 21.598585347268973 27.594385368674324 28.562740148149878 22.244289135906822 76-77 21.722967654221506 27.14202065503936 28.67732281526002 22.45768887547911 78-79 21.81199877999558 27.19901230779908 28.64595946590106 22.343029446304282 80-81 21.72703730353823 27.546469623531216 28.46556189070827 22.260931182222286 82-83 21.830092142655484 27.49166647492346 28.489352043087802 22.18888933933325 84-85 21.77955414731523 27.382130175310582 28.413439784030864 22.424875893343327 86-87 21.777938315874206 27.374659150756713 28.50463876371706 22.34276376965202 88-89 21.795071279078687 27.291285434084656 28.30044232110832 22.613200965728332 90-91 21.77498117185223 27.21768552939054 28.481344610986 22.525988687771235 92-93 21.866207265300634 26.87614862515595 28.718258501652265 22.539385607891145 94-95 21.639570226128725 26.91254705080135 28.76430150789007 22.683581215179853 96-97 21.637725296712656 26.850860936053827 28.689942487382364 22.821471279851153 98-99 21.528689639806636 27.044361694550624 28.37592477267622 23.051023892966523 100-101 21.806517629942125 26.26085711698078 29.208274050723514 22.724351202353578 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1900.0 1 1123.0 2 353.5 3 352.0 4 303.0 5 295.0 6 479.0 7 949.5 8 1596.0 9 1988.0 10 1852.5 11 1630.0 12 1711.5 13 1870.5 14 2008.5 15 2098.5 16 2071.0 17 2162.0 18 2340.0 19 2561.0 20 2816.5 21 3164.0 22 3812.5 23 4573.5 24 5445.5 25 6655.0 26 8592.5 27 10986.0 28 12801.0 29 14834.5 30 17587.5 31 20166.5 32 22926.0 33 25431.5 34 27806.0 35 30370.0 36 33921.5 37 37147.5 38 38961.0 39 40190.0 40 41103.0 41 43345.5 42 45672.0 43 47882.5 44 50079.0 45 52033.5 46 52745.5 47 53632.5 48 55144.5 49 53810.5 50 50704.5 51 47356.0 52 44983.5 53 43945.0 54 42873.5 55 40130.0 56 35949.5 57 31722.5 58 28258.0 59 24674.5 60 20439.0 61 16553.0 62 13184.5 63 9987.5 64 7290.0 65 5638.5 66 4227.5 67 3042.0 68 2230.0 69 1547.0 70 1142.5 71 844.0 72 624.0 73 514.5 74 389.5 75 287.5 76 207.0 77 134.0 78 77.5 79 51.5 80 34.0 81 29.0 82 23.0 83 15.5 84 11.0 85 5.0 86 6.5 87 7.5 88 4.5 89 3.0 90 3.0 91 2.0 92 2.0 93 4.0 94 2.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3448793797129262 2 0.28574678416383886 3 0.2915798392118376 4 0.28501765228283904 5 0.2861842632924388 6 0.20904211028265526 7 0.19978213539395723 8 0.20649014869915583 9 0.20969832897555513 10-11 0.20918793665885527 12-13 0.21261485649955453 14-15 0.20758384652065556 16-17 0.1990165469189074 18-19 0.1926001863661088 20-21 0.1926001863661088 22-23 0.20003733155230718 24-25 0.1972301738104578 26-27 0.20474023218475618 28-29 0.21166698505425469 30-31 0.21374501091510428 32-33 0.2107191136089549 34-35 0.21050037404465494 36-37 0.19770410953310769 38-39 0.1886628742087096 40-41 0.18465264886321048 42-43 0.1938761671578585 44-45 0.19642812874135795 46-47 0.2041204700859063 48-49 0.21305233562815443 50-51 0.2073286503623056 52-53 0.21863019451780322 54-55 0.20430275305615628 56-57 0.1794028993200116 58-59 0.16383593366066496 60-61 0.18162675155706112 62-63 0.1733511047077129 64-65 0.18213714387376104 66-67 0.18107990264631124 68-69 0.17903833337951167 70-71 0.18140801199276116 72-73 0.18997531159450934 74-75 0.1952250611377082 76-77 0.19507923476150826 78-79 0.1929647523066087 80-81 0.1887722439908596 82-83 0.18049659714151137 84-85 0.1756843267269124 86-87 0.1813350988046612 88-89 0.18804311210985977 90-91 0.18468910545726047 92-93 0.1798039218545615 94-95 0.18191840430946107 96-97 0.1878972857336598 98-99 0.1878608291396098 100-101 0.1710543392825634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1371494.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.8014040154629 #Duplication Level Percentage of deduplicated Percentage of total 1 78.89531462636282 44.81364641019094 2 11.881021072526915 13.497173561136592 3 3.8228226893761463 6.5142508817619875 4 1.684996638112689 3.8284069922454234 5 0.9206293430593167 2.6146519631801217 6 0.5552302424779679 1.8922714394756697 7 0.37910226729059215 1.507347873328565 8 0.27769971014526496 1.2618986744750509 9 0.2000482400371225 1.0226718814437807 >10 1.2444461045271376 13.208918122283444 >50 0.09486399360618464 3.688844264849521 >100 0.04137604879744774 4.1781720913460285 >500 0.0018045437645660208 0.6815559045125802 >1k 3.866879495498616E-4 0.4705510579334357 >5k 2.5779196636657443E-4 0.819638881836753 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6193 0.4515513739032034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5002 0.36471176687612195 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3130 0.22821827875295114 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1916 0.1397016683995701 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1381 0.10069311276607845 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.29131880999844E-5 7 0.0 0.0 0.0 0.0 7.29131880999844E-5 8 0.0 0.0 0.0 0.0 7.29131880999844E-5 9 0.0 0.0 0.0 0.0 7.29131880999844E-5 10-11 0.0 0.0 0.0 0.0 7.29131880999844E-5 12-13 0.0 0.0 0.0 0.0 7.29131880999844E-5 14-15 0.0 0.0 0.0 0.0 7.29131880999844E-5 16-17 0.0 0.0 0.0 0.0 7.29131880999844E-5 18-19 0.0 0.0 0.0 3.64565940499922E-5 7.29131880999844E-5 20-21 0.0 0.0 0.0 1.093697821499766E-4 7.29131880999844E-5 22-23 0.0 0.0 0.0 1.458263761999688E-4 7.29131880999844E-5 24-25 0.0 0.0 0.0 2.916527523999376E-4 7.29131880999844E-5 26-27 0.0 0.0 0.0 4.7393572264989856E-4 7.29131880999844E-5 28-29 0.0 0.0 0.0 0.0010572412274497739 7.29131880999844E-5 30-31 0.0 0.0 0.0 0.0022603088310995164 7.29131880999844E-5 32-33 0.0 0.0 0.0 0.004228964909799095 7.29131880999844E-5 34-35 0.0 0.0 0.0 0.005905968236098736 7.29131880999844E-5 36-37 0.0 0.0 0.0 0.008020450690998285 7.29131880999844E-5 38-39 0.0 0.0 0.0 0.013889962333047026 7.29131880999844E-5 40-41 0.0 0.0 0.0 0.01877514593574598 7.29131880999844E-5 42-43 0.0 0.0 0.0 0.02307702403364506 7.29131880999844E-5 44-45 0.0 0.0 0.0 0.027415358725594134 7.29131880999844E-5 46-47 0.0 0.0 0.0 0.03233699892234308 7.29131880999844E-5 48-49 0.0 0.0 0.0 0.03744092208934199 7.29131880999844E-5 50-51 0.0 0.0 0.0 0.04225319250394095 7.29131880999844E-5 52-53 0.0 0.0 0.0 0.052132929491488844 7.29131880999844E-5 54-55 0.0 0.0 0.0 0.06813737427943542 7.29131880999844E-5 56-57 0.0 0.0 0.0 0.09617249510387942 7.29131880999844E-5 58-59 0.0 0.0 0.0 0.13434254907422127 7.29131880999844E-5 60-61 0.0 0.0 0.0 0.18107990264631124 7.29131880999844E-5 62-63 0.0 0.0 0.0 0.21119304933160482 7.29131880999844E-5 64-65 0.0 0.0 0.0 0.24619137961959733 7.29131880999844E-5 66-67 0.0 0.0 0.0 0.3157505610669824 7.29131880999844E-5 68-69 0.0 0.0 0.0 0.4042671714203635 7.29131880999844E-5 70-71 3.64565940499922E-5 0.0 0.0 0.4977054219704935 7.29131880999844E-5 72-73 7.29131880999844E-5 0.0 0.0 0.6068929211502201 7.29131880999844E-5 74-75 7.29131880999844E-5 0.0 0.0 0.719653166546846 7.29131880999844E-5 76-77 7.29131880999844E-5 0.0 0.0 0.827054292618123 7.29131880999844E-5 78-79 7.29131880999844E-5 0.0 0.0 0.9404343001135987 7.29131880999844E-5 80-81 7.29131880999844E-5 0.0 0.0 1.0434970914929267 1.093697821499766E-4 82-83 7.29131880999844E-5 0.0 0.0 1.144372487229255 1.458263761999688E-4 84-85 7.29131880999844E-5 0.0 0.0 1.3318687504283648 1.458263761999688E-4 86-87 7.29131880999844E-5 0.0 0.0 1.575034232741813 1.458263761999688E-4 88-89 7.29131880999844E-5 0.0 0.0 1.8896910959872957 1.458263761999688E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3820 0.0 43.831017 1 GTATCAA 6505 0.0 39.779 1 ATCAACG 8125 0.0 31.83137 3 TATCAAC 8125 0.0 31.714342 2 TCAACGC 8160 0.0 31.637733 4 CAACGCA 8290 0.0 31.197815 5 AACGCAG 8410 0.0 30.784424 6 ACGCAGA 9385 0.0 27.434406 7 CGCAGAG 9405 0.0 27.275045 8 GTACATG 6090 0.0 25.540693 1 GCAGAGT 10005 0.0 24.97281 9 TACATGG 6095 0.0 24.726654 2 ACATGGG 6105 0.0 23.284416 3 GGACCGA 245 2.9649527E-10 23.267284 6 GAGTACT 5685 0.0 23.183329 12-13 GTACTTT 5920 0.0 21.983051 14-15 TTAGTCG 55 0.0028800028 21.5888 38-39 AGTACTT 6025 0.0 21.480911 12-13 AGAGTAC 9235 0.0 21.189438 10-11 CATGGGA 3325 0.0 20.733475 4 >>END_MODULE