FastQCFastQC Report
Thu 26 May 2016
SRR937856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371494
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71220.5192877256480889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60090.43813534729280623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.29537132499303675No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.12067132630547417No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15400.11228630967397596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46150.040.0499731
GTATCAA71500.035.1539341
TATCAAC88450.028.301562
ATCAACG88000.028.284353
TCAACGC88850.027.9603044
CAACGCA90250.027.526575
AACGCAG91900.027.0313646
GTACATG60100.026.4056151
TACATGG62250.025.3335672
ACATGGG61600.025.0611083
ACGCAGA105850.023.2004977
CATGGGG25850.022.9692634
CGCAGAG107300.022.7984398
GAGTACT66300.022.3889712-13
ACCGTGC2554.9112714E-1022.3531488
GCAGAGT112900.021.331029
GTAAGGT6900.020.6523634
GTACTTT71350.020.60385314-15
AGTACTT69350.020.00018512-13
ACCCGTC1200.004763704719.791858
Produced by FastQC (version 0.11.5)