##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937856_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1371494 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.284961509128 33.0 31.0 34.0 31.0 34.0 2 32.49575061939753 34.0 31.0 34.0 31.0 34.0 3 32.60755716029381 34.0 31.0 34.0 31.0 34.0 4 36.21111794874786 37.0 35.0 37.0 35.0 37.0 5 36.06693284841202 37.0 35.0 37.0 35.0 37.0 6 36.163959886080434 37.0 35.0 37.0 35.0 37.0 7 36.1299611956013 37.0 35.0 37.0 35.0 37.0 8 36.12004427288782 37.0 35.0 37.0 35.0 37.0 9 37.79345443727789 39.0 38.0 39.0 35.0 39.0 10-11 37.74599487857767 39.0 37.0 39.0 35.0 39.0 12-13 37.671078400634634 39.0 37.0 39.0 35.0 39.0 14-15 38.922037573624095 40.0 38.0 41.0 35.5 41.0 16-17 38.891904375812075 40.0 38.0 41.0 35.5 41.0 18-19 38.87410881855845 40.0 38.0 41.0 35.0 41.0 20-21 38.79170561446131 40.0 38.0 41.0 35.0 41.0 22-23 38.647415883700546 40.0 38.0 41.0 34.0 41.0 24-25 38.59364459487246 40.0 38.0 41.0 34.0 41.0 26-27 38.5239497948952 40.0 38.0 41.0 34.0 41.0 28-29 38.406345561847154 40.0 38.0 41.0 34.0 41.0 30-31 38.4671529733269 40.0 38.0 41.0 34.0 41.0 32-33 38.384362600200944 40.0 38.0 41.0 34.0 41.0 34-35 38.25277872159849 40.0 38.0 41.0 34.0 41.0 36-37 38.0857010675949 40.0 38.0 41.0 34.0 41.0 38-39 37.94149227047293 40.0 37.0 41.0 33.0 41.0 40-41 37.750669707632696 40.0 37.0 41.0 33.0 41.0 42-43 37.544738803086275 40.0 37.0 41.0 33.0 41.0 44-45 37.22816140646623 40.0 37.0 41.0 32.5 41.0 46-47 36.849618372373484 40.0 36.5 41.0 31.0 41.0 48-49 36.49672984351372 39.5 36.0 41.0 30.5 41.0 50-51 36.23084862201365 39.0 36.0 41.0 30.0 41.0 52-53 35.9942938138993 39.0 35.0 40.5 29.5 41.0 54-55 35.775703357069006 39.0 35.0 40.0 29.0 41.0 56-57 35.49720159184072 38.5 35.0 40.0 28.0 41.0 58-59 35.23050483633176 38.0 35.0 40.0 28.0 41.0 60-61 34.87529693895854 38.0 34.0 40.0 27.0 41.0 62-63 34.505785661475734 37.5 34.0 40.0 26.0 41.0 64-65 34.09794100448124 37.0 34.0 39.5 26.0 41.0 66-67 33.6678122543737 36.0 33.0 39.0 25.5 40.5 68-69 33.22348949393873 36.0 33.0 39.0 24.5 40.0 70-71 32.79642382686326 35.0 32.0 38.5 23.5 40.0 72-73 32.34958629057072 35.0 32.0 37.5 22.0 39.5 74-75 31.858037293637448 35.0 31.5 37.0 20.5 39.0 76-77 30.654180404726525 34.0 30.0 35.5 20.0 38.0 78-79 31.20694366872914 34.0 31.0 36.0 20.0 38.5 80-81 30.997484495010553 34.0 31.0 36.0 19.5 37.0 82-83 30.641070613506148 34.0 31.0 35.0 18.0 37.0 84-85 30.2460608650129 34.0 30.5 35.0 15.0 36.5 86-87 29.827120643619295 34.0 30.0 35.0 8.5 36.0 88-89 29.408208129237167 34.0 29.5 35.0 4.5 36.0 90-91 28.90432477283896 34.0 29.0 35.0 2.0 35.0 92-93 28.459144188745995 34.0 29.0 35.0 2.0 35.0 94-95 27.99454645809606 33.0 28.5 35.0 2.0 35.0 96-97 27.440903861044962 33.0 27.0 35.0 2.0 35.0 98-99 26.62064471299182 33.0 25.5 34.5 2.0 35.0 100-101 24.183173240276663 30.5 19.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15287751589463738 1101 2 0.10824082676577262 1101 3 0.147936090877252 1101 4 0.03088734030906437 1101 5 -0.06744379276197776 1101 6 0.015572251650262103 1101 7 0.03088313221745409 1101 8 -0.0826416741009055 1101 9 0.15306563783360616 1101 10-11 0.12295730701261931 1101 12-13 0.14045945007116956 1101 14-15 0.2754834653788123 1101 16-17 0.08205643596039636 1101 18-19 0.21371209812629388 1101 20-21 0.19730374395603434 1101 22-23 0.24160701366886173 1101 24-25 0.17584735881450797 1101 26-27 0.04117493761359725 1101 28-29 0.1491793254230629 1101 30-31 0.10376037275928951 1101 32-33 0.11557304117390998 1101 34-35 0.10896001296357838 1101 36-37 0.013896216519086124 1101 38-39 0.18407173581467617 1101 40-41 0.15609621248965055 1101 42-43 0.18443027683155577 1101 44-45 0.113576164909297 1101 46-47 -0.0639091474238569 1101 48-49 0.025393626447105078 1101 50-51 0.17019722676321436 1101 52-53 0.15241268984487988 1101 54-55 0.07239341497045615 1101 56-57 0.24621642463336002 1101 58-59 0.26423319731448913 1101 60-61 0.2977513016477715 1101 62-63 0.39170763182599444 1101 64-65 0.18260567037255981 1101 66-67 0.32180074527377656 1101 68-69 0.3849943814859955 1101 70-71 0.45076380534854366 1101 72-73 0.24654097730361002 1101 74-75 0.26157828660225135 1101 76-77 0.20432864467626288 1101 78-79 0.3145801176512144 1101 80-81 0.3827871279032067 1101 82-83 0.16548739096782583 1101 84-85 0.2951011695772543 1101 86-87 0.5199862238712996 1101 88-89 0.7861647902223936 1101 90-91 0.5187406068441618 1101 92-93 0.8730786255221368 1101 94-95 0.8216882924795037 1101 96-97 0.9323614409899861 1101 98-99 1.28489009643328 1101 100-101 1.928923754976573 1102 1 0.12646857318387816 1102 2 0.21286131689511478 1102 3 0.14102035872863894 1102 4 0.08085867777457878 1102 5 -0.030487485212979948 1102 6 -0.02673454495322858 1102 7 0.01990851647455827 1102 8 0.055437846518202605 1102 9 0.17285756574733568 1102 10-11 0.09474172892513621 1102 12-13 0.13542111393129375 1102 14-15 0.199948506043782 1102 16-17 0.2441629978893829 1102 18-19 0.30484350688985984 1102 20-21 0.1233458957958149 1102 22-23 0.206769046559117 1102 24-25 0.09662971165030854 1102 26-27 0.10516158266393205 1102 28-29 0.02738236365171076 1102 30-31 0.20208070345328366 1102 32-33 0.2589981244743953 1102 34-35 0.19577054465359112 1102 36-37 0.3226344827940295 1102 38-39 0.22994567440519376 1102 40-41 0.20912570802904185 1102 42-43 0.2798762927392602 1102 44-45 0.27734649739469575 1102 46-47 0.44543730200468445 1102 48-49 0.4496333975051101 1102 50-51 0.4621419349427853 1102 52-53 0.29312110229875543 1102 54-55 0.3691605945770675 1102 56-57 0.4069261358583205 1102 58-59 0.5627380430742264 1102 60-61 0.5249813811863078 1102 62-63 0.43545807967809225 1102 64-65 0.6037306300658685 1102 66-67 0.5378909516440231 1102 68-69 0.355850405992463 1102 70-71 0.43231677729508533 1102 72-73 0.3593188695324798 1102 74-75 0.5040292915823699 1102 76-77 0.4255250367797352 1102 78-79 0.593048732175955 1102 80-81 0.5124358327149281 1102 82-83 0.45647302387241595 1102 84-85 0.5157643937541003 1102 86-87 0.6364864388403078 1102 88-89 0.8422493088709544 1102 90-91 0.8048395015451781 1102 92-93 1.0732635884399855 1102 94-95 1.126231528121366 1102 96-97 1.250770401614826 1102 98-99 1.4396848010300332 1102 100-101 2.2693688303948676 1103 1 0.3011714997365047 1103 2 0.22979447803253805 1103 3 0.25269136580077856 1103 4 0.11942369610167702 1103 5 0.037643861171666515 1103 6 0.1075265225734725 1103 7 0.1605452625806194 1103 8 0.18258010799964808 1103 9 0.2717680850843749 1103 10-11 0.2425568834499927 1103 12-13 0.2900103952433426 1103 14-15 0.34985253354101786 1103 16-17 0.222700309989726 1103 18-19 0.3525117098605435 1103 20-21 0.37410094373640845 1103 22-23 0.305463982842916 1103 24-25 0.2390616305220945 1103 26-27 0.2803461471202269 1103 28-29 0.21233918109801664 1103 30-31 0.32618222937549746 1103 32-33 0.2861638718272914 1103 34-35 0.36318828576928297 1103 36-37 0.40070547606885043 1103 38-39 0.21561244488989928 1103 40-41 0.20078746278379356 1103 42-43 0.16777259779790654 1103 44-45 0.15708006717613188 1103 46-47 0.16089328806128123 1103 48-49 0.14423971241463107 1103 50-51 0.30347483677184073 1103 52-53 0.29186970608145657 1103 54-55 0.22137029551010556 1103 56-57 0.3339139891482219 1103 58-59 0.29667152581084366 1103 60-61 0.32288317473586403 1103 62-63 0.27640496102828394 1103 64-65 0.43751634964151975 1103 66-67 0.5165086635418774 1103 68-69 0.28151077492861987 1103 70-71 0.26991628789619426 1103 72-73 0.4351150811548976 1103 74-75 0.3856625285035733 1103 76-77 0.14965278774630875 1103 78-79 0.19199501293711663 1103 80-81 0.2500806411199292 1103 82-83 0.21110273334761942 1103 84-85 0.13063287794653888 1103 86-87 0.1830470521715597 1103 88-89 0.45812925802378857 1103 90-91 0.5802410186411677 1103 92-93 0.667485049555399 1103 94-95 0.6234193105985746 1103 96-97 0.6539974516574887 1103 98-99 0.9016463126070988 1103 100-101 1.8107252688207964 1104 1 0.11172987602131457 1104 2 0.18327664338468708 1104 3 0.06811781309109932 1104 4 0.0249681727626907 1104 5 0.0062234519974850855 1104 6 -0.07533786748712146 1104 7 0.0930061151762871 1104 8 0.03199978269294235 1104 9 0.21213101258855005 1104 10-11 0.09279217354859526 1104 12-13 0.1126732941369184 1104 14-15 0.16822117462326247 1104 16-17 0.15207698338914355 1104 18-19 0.02151917850632401 1104 20-21 0.0665749700154521 1104 22-23 0.1271842267588923 1104 24-25 0.009651649268434426 1104 26-27 0.03426515779154471 1104 28-29 0.0073869864093296655 1104 30-31 0.07576214473937881 1104 32-33 -0.0027518721121779777 1104 34-35 -0.0513711965070911 1104 36-37 0.02205015257699472 1104 38-39 -0.04462491627399601 1104 40-41 -0.02368719122019769 1104 42-43 0.06651725466295488 1104 44-45 0.10816502782589765 1104 46-47 0.19700455337541456 1104 48-49 0.18861655662355048 1104 50-51 0.16048432524006273 1104 52-53 0.16111235806988589 1104 54-55 0.09679678550281068 1104 56-57 0.2315419788484121 1104 58-59 0.24786217484427198 1104 60-61 0.11040682117725709 1104 62-63 0.27238079575300134 1104 64-65 0.10467907559047518 1104 66-67 0.2863300394978765 1104 68-69 0.2189843528326989 1104 70-71 0.36322044838880885 1104 72-73 0.32692006793850936 1104 74-75 0.395973551268213 1104 76-77 0.3273587306361456 1104 78-79 0.38219246872948176 1104 80-81 0.349383418055524 1104 82-83 0.2231069053698782 1104 84-85 0.31271634828333106 1104 86-87 0.3808311190605629 1104 88-89 0.42326703724396 1104 90-91 0.5008973328281776 1104 92-93 0.5772880207868063 1104 94-95 0.7846058388526025 1104 96-97 1.1072954630077803 1104 98-99 1.2144406921202062 1104 100-101 2.2420609137761502 1105 1 0.08935204835010069 1105 2 0.15894113374965713 1105 3 0.10476951172792326 1105 4 0.053557696168013535 1105 5 -0.0193094267240852 1105 6 0.022023962277557985 1105 7 0.12625262367441792 1105 8 0.04442592294235226 1105 9 0.09036165512164729 1105 10-11 0.08712198770899704 1105 12-13 0.0193876319995141 1105 14-15 0.20666782737743716 1105 16-17 0.0754832950305584 1105 18-19 0.08696436011280184 1105 20-21 0.18579538268446782 1105 22-23 0.17720957052146247 1105 24-25 0.15444204920510884 1105 26-27 0.11830034658883193 1105 28-29 0.02588793308957804 1105 30-31 0.14930935890462393 1105 32-33 0.14161466576492643 1105 34-35 0.09231446826915146 1105 36-37 0.2544334888057094 1105 38-39 -0.1018691091988515 1105 40-41 -0.11699346921653841 1105 42-43 -0.16922399266798038 1105 44-45 0.09057585186651096 1105 46-47 0.012865350697886413 1105 48-49 -0.21405102557050526 1105 50-51 -0.209678919377275 1105 52-53 -0.3931527058007873 1105 54-55 -0.29158015816037164 1105 56-57 -0.21663827302790395 1105 58-59 -0.22194823621946824 1105 60-61 -0.2523696237470645 1105 62-63 -0.22821981293710536 1105 64-65 -0.13493053150974532 1105 66-67 -0.1078077997841902 1105 68-69 -0.298764252435717 1105 70-71 -0.13000627987987912 1105 72-73 -0.2108718251361026 1105 74-75 -0.258220154242764 1105 76-77 -0.2731751926222792 1105 78-79 -0.17127792249904772 1105 80-81 -0.0024624984650927217 1105 82-83 0.05527209832332147 1105 84-85 0.06431366503308311 1105 86-87 0.14137461907265703 1105 88-89 0.2310541144539222 1105 90-91 0.302822634149571 1105 92-93 0.4132808915479096 1105 94-95 0.6171852731314438 1105 96-97 0.931393532923579 1105 98-99 1.2047036845093757 1105 100-101 1.78924154388476 1106 1 -0.17392890694211616 1106 2 -0.01569985120033124 1106 3 -0.02464419458215872 1106 4 0.021186180630209606 1106 5 -0.08791658491184506 1106 6 -0.07057850660129361 1106 7 -0.12305308544534199 1106 8 -0.053538195064334104 1106 9 -0.19580813558298615 1106 10-11 -0.07791802751012256 1106 12-13 -0.17201267303320122 1106 14-15 -0.10434481294411313 1106 16-17 -0.03946452746512108 1106 18-19 3.479143617965974E-4 1106 20-21 -0.16134924444717313 1106 22-23 -0.07079820488599609 1106 24-25 -0.2420663343166254 1106 26-27 -0.4720164093980017 1106 28-29 -0.3916101482611509 1106 30-31 -0.3221149617634609 1106 32-33 -0.41173516578417235 1106 34-35 -0.5407946703548916 1106 36-37 -0.4974441046227511 1106 38-39 -0.4819519037928117 1106 40-41 -0.45389486690375236 1106 42-43 -0.27211521826210827 1106 44-45 -0.4376392677401313 1106 46-47 -0.4308304230705957 1106 48-49 -0.6267138668624384 1106 50-51 -0.8951740934865668 1106 52-53 -0.7482898079249622 1106 54-55 -0.8608637215148249 1106 56-57 -0.9890868759910845 1106 58-59 -0.8200330827925555 1106 60-61 -0.6375185467019122 1106 62-63 -0.6857957134014825 1106 64-65 -0.8087357658451069 1106 66-67 -0.8390099148511112 1106 68-69 -1.0842852795309526 1106 70-71 -0.8829505122250509 1106 72-73 -0.6832148210906794 1106 74-75 -0.5975430336475256 1106 76-77 -0.945035762103398 1106 78-79 -0.6891888386378149 1106 80-81 -0.5606227739701204 1106 82-83 -0.49220105684662485 1106 84-85 -0.4894438794860818 1106 86-87 -0.4452958903124653 1106 88-89 -0.43498262280169087 1106 90-91 -0.28540189984629194 1106 92-93 -0.15762498732353336 1106 94-95 -0.08859270662843599 1106 96-97 0.12102111984814812 1106 98-99 0.2340760910809152 1106 100-101 1.0304848611317894 1107 1 0.18657831097780075 1107 2 0.12868800007439063 1107 3 0.11787536213231675 1107 4 0.14455252737310786 1107 5 0.029130154850335543 1107 6 0.1424404532014094 1107 7 0.025764479468811885 1107 8 0.058464247561452964 1107 9 0.09750920156220388 1107 10-11 0.1327726818723889 1107 12-13 0.12522238846442235 1107 14-15 0.181265195538316 1107 16-17 0.183118274407299 1107 18-19 0.22868728226364965 1107 20-21 0.24390573898066492 1107 22-23 0.31043353432639265 1107 24-25 0.31614630764627094 1107 26-27 0.13919424154999405 1107 28-29 0.06344082486211278 1107 30-31 0.1603262110110819 1107 32-33 0.0578203705724718 1107 34-35 0.22563267527273467 1107 36-37 0.1503715713798215 1107 38-39 0.05089591512300018 1107 40-41 0.03912234536121417 1107 42-43 -0.00821002120268588 1107 44-45 0.06775797311377119 1107 46-47 0.2393836797553064 1107 48-49 0.02894314842164647 1107 50-51 -0.010066845721063089 1107 52-53 -0.029264423466656808 1107 54-55 -0.09293967853717078 1107 56-57 0.18568309699080743 1107 58-59 0.04480518160793423 1107 60-61 0.164716632607238 1107 62-63 0.16416049997876314 1107 64-65 0.07346251881897103 1107 66-67 0.3191658128314572 1107 68-69 0.14223488194939193 1107 70-71 0.2760101347958823 1107 72-73 0.25156176897231575 1107 74-75 0.388062360549867 1107 76-77 0.1406701646021169 1107 78-79 0.1843041555030247 1107 80-81 0.11686717277906666 1107 82-83 0.09523228146097296 1107 84-85 0.14140104519307783 1107 86-87 0.3518243049405143 1107 88-89 0.32790055920926875 1107 90-91 0.3060738466861217 1107 92-93 0.5737023755831139 1107 94-95 0.7229574378870431 1107 96-97 0.7120423102158746 1107 98-99 1.2948030275098112 1107 100-101 2.1733892688539456 1108 1 0.08863631179148257 1108 2 0.16869164643790668 1108 3 0.06855491936924807 1108 4 0.07769493306143715 1108 5 -0.09946493167483084 1108 6 0.015541094522468768 1108 7 -0.004713886285081514 1108 8 -0.002549880409027594 1108 9 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48-49 -0.6313072213427589 2316 50-51 -0.6750106777746723 2316 52-53 -0.580845910566758 2316 54-55 -0.8539376886265231 2316 56-57 -0.49431148382493717 2316 58-59 -0.48619376044303664 2316 60-61 -0.5591198391931584 2316 62-63 -0.5801100933528787 2316 64-65 -0.576730431754271 2316 66-67 -0.5264724341832405 2316 68-69 -0.27620208033896176 2316 70-71 -0.4705951086963438 2316 72-73 -0.3533395725346722 2316 74-75 -0.34420922687417743 2316 76-77 -0.5327523609103295 2316 78-79 -0.729658096599497 2316 80-81 -0.7290549773599508 2316 82-83 -0.48342959423260723 2316 84-85 -0.6013404407334413 2316 86-87 -0.57625630283653 2316 88-89 -0.6222567524611335 2316 90-91 -0.8229989550493997 2316 92-93 -0.7646786517492501 2316 94-95 -0.8665522985231426 2316 96-97 -1.084941068075974 2316 98-99 -1.5236784618338959 2316 100-101 -2.2698792931465306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 11.0 5 25.0 6 42.0 7 100.0 8 284.0 9 538.0 10 989.0 11 1671.0 12 2676.0 13 3946.0 14 5188.0 15 7233.0 16 10249.0 17 14936.0 18 13283.0 19 7964.0 20 6535.0 21 6458.0 22 6548.0 23 7178.0 24 8237.0 25 9680.0 26 11442.0 27 14458.0 28 17903.0 29 22781.0 30 29000.0 31 36799.0 32 48358.0 33 63962.0 34 90601.0 35 133639.0 36 211577.0 37 319359.0 38 239853.0 39 17986.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2818041628917 19.605229948193635 15.21897709052765 21.893988798387014 2 15.537873297294775 20.394620756634733 40.642467265624205 23.425038680446285 3 19.71805928425498 24.744184079551204 29.81872323174582 25.719033404447995 4 13.12590503494729 17.122131048331234 36.57996316425737 33.1720007524641 5 13.532505491235899 37.819155297693534 33.937442207112205 14.710897003958356 6 31.83834562892729 36.63442931576806 16.938681467071675 14.588543588232977 7 28.756669733881445 33.218519366471895 20.065928104680005 17.95888279496666 8 26.665738238738196 35.5152118784333 18.995270850619836 18.82377903220867 9 26.647364115337 17.20364799262702 20.17989141768028 35.9690964743557 10-11 25.140248517310322 27.15221502974129 26.546598089382815 21.16093836356557 12-13 26.05810547622676 24.50032338342921 27.583330477448108 21.858240662895927 14-15 22.72456168236974 25.893915686105807 26.162382044689952 25.219140586834506 16-17 21.763748146182195 29.25929679604869 26.987066658694825 21.989888399074296 18-19 21.757696351569894 27.17299528834978 30.01234420274533 21.056964157334992 20-21 22.317783230884043 26.182881920886857 30.49074675271411 21.008588095514995 22-23 22.19445843975644 26.04676224634358 30.331515117488923 21.42726419641106 24-25 22.06213251592431 26.36402943372454 30.408651041788186 21.165187008562963 26-27 21.619262750496546 26.789203748370372 30.256307802688493 21.335225698444596 28-29 21.417454540416802 26.92357190173577 30.373421715675676 21.28555184217175 30-31 21.550487753838706 26.612205256906535 30.313177410569956 21.524129578684803 32-33 21.541107726318888 26.706460254292036 30.209975399090332 21.54245662029874 34-35 22.073592739013076 26.657025112760245 29.984272625326835 21.285109522899845 36-37 22.10852621612862 26.360132220823502 29.943525069568324 21.587816493479554 38-39 21.7302080796562 26.709595521380336 30.029843367889324 21.530353031074142 40-41 21.941182389423506 26.73733898945238 29.886605409866906 21.434873211257212 42-43 21.811852355810583 26.801444838334376 29.682699238605053 21.70400356724999 44-45 21.848254531190076 27.086228594510803 29.57891175608497 21.486605118214154 46-47 22.1946650878531 27.36760058738864 28.857909695558277 21.57982462919998 48-49 22.2429781840016 27.483178009957758 28.62474375562115 21.649100050419488 50-51 22.361964397948515 27.633004592072584 28.420321197176218 21.58470981280268 52-53 22.54364404338921 27.697480045468698 28.20182093528731 21.55705497585478 54-55 22.132761791156213 27.834755383545247 28.40260329246792 21.62987953283062 56-57 22.24559494974094 27.99986729799766 28.282806924419646 21.471730827841757 58-59 22.27745801294063 27.492245682445564 28.338549056722083 21.891747247891715 60-61 22.084238064475674 27.71794845620907 28.754992730555145 21.44282074876011 62-63 21.995757910716343 27.74481696602391 28.87533594751417 21.384089175745576 64-65 21.95988462217115 27.70307416583667 28.665819901508865 21.67122131048331 66-67 21.860649773167072 27.921850186730673 28.391301748312426 21.82619829178983 68-69 21.929443366139406 27.83978639352414 28.527576496871294 21.703193743465153 70-71 22.06065064812533 27.86691009949734 28.277192608935948 21.795246643441384 72-73 22.16755754495284 27.783335387056297 28.224788606285266 21.8243184617056 74-75 22.09417036762127 28.35318882415003 27.854972646597542 21.697668161631157 76-77 22.206258284761 27.759035037703413 28.159765919500924 21.87494075803467 78-79 22.245813689305237 27.959619218166466 28.0394227025419 21.755144389986395 80-81 22.164223831821356 28.336799140207685 27.832166965367694 21.666810062603265 82-83 22.142005358403868 28.477229700096355 27.684005102477926 21.696759839021855 84-85 22.10599421067598 28.356094466602016 27.723388431559837 21.814522891162166 86-87 21.998783808022495 28.344600851334384 27.8116783595116 21.844936981131525 88-89 22.05904655798713 28.350616189352635 27.57135649153405 22.01898076112619 90-91 22.139179956102932 28.30358249659105 27.620881003944902 21.936356543361114 92-93 22.17894704605908 27.969398945354428 27.952081583368333 21.89957242521816 94-95 22.004616863070492 27.98980527803986 27.981128608655965 22.024449250233687 96-97 22.072134475251076 28.068952543722393 27.75713929481281 22.101773686213722 98-99 21.829608006763447 28.24208406839129 27.613388499592233 22.314919425253038 100-101 21.585252246454623 28.419177767528183 27.980260003536717 22.01530998248048 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 624.0 1 405.5 2 248.0 3 299.5 4 261.5 5 285.0 6 508.5 7 1005.5 8 1602.5 9 2000.5 10 1991.0 11 1870.0 12 2014.0 13 2281.0 14 2427.0 15 2502.0 16 2669.5 17 2811.5 18 2839.0 19 2891.0 20 3122.5 21 3431.0 22 3923.5 23 4673.0 24 5532.5 25 6724.5 26 8557.0 27 10897.0 28 12826.5 29 15191.5 30 18049.5 31 20132.0 32 22361.5 33 24841.5 34 27853.0 35 30374.5 36 33975.0 37 37494.5 38 39123.5 39 40351.0 40 41279.0 41 43632.5 42 45798.5 43 47636.0 44 50239.5 45 51950.5 46 51804.0 47 52820.5 48 54970.0 49 53473.0 50 50692.0 51 47620.0 52 44486.0 53 43553.5 54 42994.5 55 40185.0 56 35464.5 57 31601.0 58 28712.5 59 25185.0 60 20578.5 61 16500.0 62 13243.5 63 9858.0 64 6941.0 65 5134.0 66 3886.5 67 2978.0 68 2154.5 69 1446.5 70 1047.0 71 745.5 72 552.5 73 473.0 74 390.0 75 296.5 76 196.5 77 119.0 78 70.5 79 52.5 80 33.5 81 15.0 82 6.5 83 4.5 84 3.0 85 2.0 86 2.0 87 2.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04520617662199033 2 0.0 3 0.0 4 0.0 5 0.015895075005796597 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004156051721699111 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.82282970249961E-4 22-23 2.187395642999532E-4 24-25 4.374791285999064E-4 26-27 0.001312437385799719 28-29 0.0020780258608495555 30-31 2.187395642999532E-4 32-33 0.0 34-35 0.0 36-37 3.64565940499922E-5 38-39 0.0 40-41 0.0 42-43 0.00944225785894798 44-45 0.0 46-47 0.0 48-49 3.64565940499922E-5 50-51 0.0 52-53 7.29131880999844E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 7.29131880999844E-5 74-75 7.29131880999844E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.5519615834994537E-4 84-85 2.916527523999376E-4 86-87 0.0 88-89 0.0 90-91 0.007582971562398377 92-93 0.002770701147799407 94-95 0.0 96-97 0.0 98-99 2.5519615834994537E-4 100-101 0.012140045818647402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1371494.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.126655574586216 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14112554228133 41.75391132862976 2 12.787706739684948 13.84311596575484 3 4.09395159982621 6.647757245484582 4 1.8498206597150983 4.004984228926118 5 1.009084968281132 2.730919726182254 6 0.6419137588420972 2.084678696006051 7 0.42820717741518566 1.6224195684562237 8 0.30972884197836703 1.3411669081022808 9 0.22112826350902523 1.0772040021083416 >10 1.3626624239462144 13.690027038047662 >50 0.10046059565516245 3.7385030810530164 >100 0.05094053806722468 4.9676658504043045 >500 0.0023154643152298563 0.8262823699739006 >1k 6.810189162440753E-4 0.7033131521726455 >5k 2.724075664976302E-4 0.9680508386980091 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7122 0.5192877256480889 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6009 0.43813534729280623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4051 0.29537132499303675 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1655 0.12067132630547417 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1540 0.11228630967397596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.64565940499922E-5 0.0 20-21 0.0 0.0 0.0 1.093697821499766E-4 0.0 22-23 0.0 0.0 0.0 1.458263761999688E-4 0.0 24-25 0.0 0.0 0.0 2.916527523999376E-4 0.0 26-27 0.0 0.0 0.0 4.7393572264989856E-4 0.0 28-29 0.0 0.0 0.0 0.0011301544155497582 0.0 30-31 0.0 0.0 0.0 0.0022603088310995164 0.0 32-33 0.0 0.0 0.0 0.004338334691949072 7.29131880999844E-5 34-35 0.0 0.0 0.0 0.006088251206348697 7.29131880999844E-5 36-37 0.0 0.0 0.0 0.008421473225548197 7.29131880999844E-5 38-39 0.0 0.0 0.0 0.01443681124379691 7.29131880999844E-5 40-41 0.0 0.0 0.0 0.019577191004845812 7.29131880999844E-5 42-43 0.0 0.0 0.0 0.02409780866704484 7.29131880999844E-5 44-45 0.0 0.0 0.0 0.02836323017089393 7.29131880999844E-5 46-47 0.0 0.0 0.0 0.03324841377359289 7.29131880999844E-5 48-49 0.0 0.0 0.0 0.038206510564391824 7.29131880999844E-5 50-51 0.0 0.0 0.0 0.043128150761140766 7.29131880999844E-5 52-53 0.0 0.0 0.0 0.053153714124888625 7.29131880999844E-5 54-55 0.0 0.0 0.0 0.0690852457247352 7.29131880999844E-5 56-57 0.0 0.0 0.0 0.09744847589562913 7.29131880999844E-5 58-59 0.0 0.0 0.0 0.13576435624217095 7.29131880999844E-5 60-61 0.0 0.0 0.0 0.18246525322021095 7.29131880999844E-5 62-63 0.0 0.0 0.0 0.21276068287575448 7.29131880999844E-5 64-65 0.0 0.0 0.0 0.24837877526259683 7.29131880999844E-5 66-67 0.0 0.0 0.0 0.3186670885909818 7.29131880999844E-5 68-69 0.0 0.0 0.0 0.40798574401346266 7.29131880999844E-5 70-71 0.0 0.0 0.0 0.5013146247814427 1.458263761999688E-4 72-73 0.0 0.0 0.0 0.6110854294659692 1.458263761999688E-4 74-75 0.0 0.0 0.0 0.724647719931695 1.458263761999688E-4 76-77 0.0 0.0 0.0 0.8332519136066217 1.458263761999688E-4 78-79 0.0 0.0 0.0 0.9470694002306973 1.458263761999688E-4 80-81 0.0 0.0 0.0 1.051553998777975 1.458263761999688E-4 82-83 0.0 0.0 0.0 1.1543615939989529 1.458263761999688E-4 84-85 0.0 0.0 0.0 1.3441910792172624 1.458263761999688E-4 86-87 0.0 0.0 0.0 1.5903460022428098 1.458263761999688E-4 88-89 0.0 0.0 0.0 1.9102890716255412 1.458263761999688E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4615 0.0 40.049973 1 GTATCAA 7150 0.0 35.153934 1 TATCAAC 8845 0.0 28.30156 2 ATCAACG 8800 0.0 28.28435 3 TCAACGC 8885 0.0 27.960304 4 CAACGCA 9025 0.0 27.52657 5 AACGCAG 9190 0.0 27.031364 6 GTACATG 6010 0.0 26.405615 1 TACATGG 6225 0.0 25.333567 2 ACATGGG 6160 0.0 25.061108 3 ACGCAGA 10585 0.0 23.200497 7 CATGGGG 2585 0.0 22.969263 4 CGCAGAG 10730 0.0 22.798439 8 GAGTACT 6630 0.0 22.38897 12-13 ACCGTGC 255 4.9112714E-10 22.353148 8 GCAGAGT 11290 0.0 21.33102 9 GTAAGGT 690 0.0 20.652363 4 GTACTTT 7135 0.0 20.603853 14-15 AGTACTT 6935 0.0 20.000185 12-13 ACCCGTC 120 0.0047637047 19.79185 8 >>END_MODULE