FastQCFastQC Report
Thu 26 May 2016
SRR937855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732937
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88411.2062428285104996No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68310.9320037056390932No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48790.6656779504923342No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31710.4326429147389203No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27100.36974528506542853No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26040.35528292336176237No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25400.34655093138973747No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25120.34273068490197656No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24550.3349537545518919No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24060.32826832319831034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23950.32676751207811855No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22670.3093035281340688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.2902022956952644No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC17570.23972046710699557No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA17300.23603665799379758No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT15790.21543461443480136No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA15200.2073848093355909No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG15200.2073848093355909No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC12450.1698645313307965No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC12370.1687730323342934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.16481634847196963No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC10600.14462361703666207No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9470.12920619371105566No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG8400.11460739463282656No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG8070.11010496127225122No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT7740.10560252791167589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGT550.002578996134.5455066
GTACATG20050.025.1483671
TACTATA1151.2728122E-424.8063932
TACATGG20400.023.5397242
ACATGGG21050.021.9093673
ACTATAC1100.00286040821.611633
TATAAGC4200.021.5087173
GTTCTAG1553.8608465E-521.4824921
GAGTACT21600.021.21819512-13
CATGGGG14400.020.804044
ATATAGC1150.003701370420.6719953
GTACTTT22700.020.39922314-15
TATAACT2108.5088504E-720.3794694
GTTCCGG1200.004722404819.8201581
CCCTAAT1456.083871E-419.6834661
AGTACTT21800.019.2806512-13
TCTAAAG4500.019.0182343
ATCGGAC2001.2844308E-518.998738
CCTATAA3801.0913936E-1118.776991
GGATCGG2051.588086E-518.5366126
Produced by FastQC (version 0.11.5)